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The map label for this gene is glcF [H]

Identifier: 222109407

GI number: 222109407

Start: 209111

End: 210349

Strand: Direct

Name: glcF [H]

Synonym: Dtpsy_0186

Alternate gene names: 222109407

Gene position: 209111-210349 (Clockwise)

Preceding gene: 222109406

Following gene: 222109408

Centisome position: 5.51

GC content: 69.98

Gene sequence:

>1239_bases
ATGCAGACCCAGCTCGCACCCCAGTACCAGGGCACGCCCGAAGGGCAGGAGGCCGAGGCCATCCTGCGCAAATGCGTGCA
TTGCGGCTTTTGCACTGCCACCTGCCCCACCTACCAGTTGCTGGGCGATGAGCTGGACGGGCCGCGCGGGCGCATCTACC
TCATCAAGCAGGTGCTGGAAGGGGCCGAGCCCACGCGCAAGACGCAGTTGCACCTGGACCGGTGCCTGACCTGCCGCAAC
TGCGAGAGCACCTGCCCGAGCGGCGTGCAGTACGGGCATCTGGTGGACATCGGCCGGCGCATCGTCGAGGAAAAGGTGCC
GCGCCCGCGCGGCGAGGCGGCGGCCCGCTGGATGCTCAAGGAAGGCTTGCCATCGCCGCTCTTTGCCCCGGCAATGAAGG
TGGGCCAGGCGGTGCGCGGCCTGCTGCCCGAGCGCCTGCGCGCCAAGGTGCCGCCGCGCCAGGATGCCGGCGCCTGGCCC
ACGCGCAGCCATGCGCGCAAGGTGCTGATGCTGGAGGGCTGCGTGCAGCCGTCCATGGCGCCCAACATCAACAATGCCAC
GGCGCGCGTGCTGGATGCTGCAGGCATACAGACCGTGGTGGCGCGCGAGGCGGGCTGCTGCGGCGCGGTCAAGTTCCACC
TGAACGATCAGGAAGGGGGCCTCGCCCAGATGCGCGCCAACATCGACGCCTGGTGGCCCTATGTGGAACGCGGCGAGGTG
GAGGCCATCGTCATGAATGCCTCGGGCTGCGGCGTCACCGTGAAGGAATACGGCCACCTGCTGCGCAACGACGCGCAGTA
CGCCGAGAAGGCGGCGCGGGTGAGCGCCCTTACGCGCGATCTGTCGGAACTGCTGCCCGATCTGGTGGAGCGCCTTCGCG
GGCGCATCCAGCCTCCCGCGGCGCCGCTGGCCTACCACCCGCCCTGCACCCTGCAGCACGGGCAGAAGCTGCGCGGCGGC
GTGGAGCGGCACCTGGCGGCGCTGGGCTTCGAGCTGCGCGTGGCGCGCACCGAGTCGCACCTGTGCTGCGGCTCGGCCGG
CACCTACTCGGTGCTCAACCCGGAACTGTCGTACCAGCTGCGCGACCGCAAGCTGGGCGCGCTGGGCGAGGCCTTCACCG
AGCAGCCGCCTGCGGCCATCCTCTCGGCCAACATGGGCTGCATCACCCACCTGCAAAGCGGCACGGGCATTCCCGTGAAG
CACTGGGTGGAGGTGCTGGACGCTGCATTGCTGGCATGA

Upstream 100 bases:

>100_bases
CGCCAGCGCCTGGCCCAGCTGTGGCGCGCGCGCCGCGCCCCCGCACCCGCCGAAGACGCCCCCGCGCGCCGCTAACCCTC
CACGATCTCCACGACACACC

Downstream 100 bases:

>100_bases
TGGCGGGTTTTGACCGCCGCGCGGGGCGGGCTTTCGGGGCCCGGCCGCGCGCTTTCGAGCCATGAACGACACCGTGAACG
CACCCGCTCCGATCCACGAA

Product: glycolate oxidase iron-sulfur subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MQTQLAPQYQGTPEGQEAEAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLEGAEPTRKTQLHLDRCLTCRN
CESTCPSGVQYGHLVDIGRRIVEEKVPRPRGEAAARWMLKEGLPSPLFAPAMKVGQAVRGLLPERLRAKVPPRQDAGAWP
TRSHARKVLMLEGCVQPSMAPNINNATARVLDAAGIQTVVAREAGCCGAVKFHLNDQEGGLAQMRANIDAWWPYVERGEV
EAIVMNASGCGVTVKEYGHLLRNDAQYAEKAARVSALTRDLSELLPDLVERLRGRIQPPAAPLAYHPPCTLQHGQKLRGG
VERHLAALGFELRVARTESHLCCGSAGTYSVLNPELSYQLRDRKLGALGEAFTEQPPAAILSANMGCITHLQSGTGIPVK
HWVEVLDAALLA

Sequences:

>Translated_412_residues
MQTQLAPQYQGTPEGQEAEAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLEGAEPTRKTQLHLDRCLTCRN
CESTCPSGVQYGHLVDIGRRIVEEKVPRPRGEAAARWMLKEGLPSPLFAPAMKVGQAVRGLLPERLRAKVPPRQDAGAWP
TRSHARKVLMLEGCVQPSMAPNINNATARVLDAAGIQTVVAREAGCCGAVKFHLNDQEGGLAQMRANIDAWWPYVERGEV
EAIVMNASGCGVTVKEYGHLLRNDAQYAEKAARVSALTRDLSELLPDLVERLRGRIQPPAAPLAYHPPCTLQHGQKLRGG
VERHLAALGFELRVARTESHLCCGSAGTYSVLNPELSYQLRDRKLGALGEAFTEQPPAAILSANMGCITHLQSGTGIPVK
HWVEVLDAALLA
>Mature_412_residues
MQTQLAPQYQGTPEGQEAEAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLEGAEPTRKTQLHLDRCLTCRN
CESTCPSGVQYGHLVDIGRRIVEEKVPRPRGEAAARWMLKEGLPSPLFAPAMKVGQAVRGLLPERLRAKVPPRQDAGAWP
TRSHARKVLMLEGCVQPSMAPNINNATARVLDAAGIQTVVAREAGCCGAVKFHLNDQEGGLAQMRANIDAWWPYVERGEV
EAIVMNASGCGVTVKEYGHLLRNDAQYAEKAARVSALTRDLSELLPDLVERLRGRIQPPAAPLAYHPPCTLQHGQKLRGG
VERHLAALGFELRVARTESHLCCGSAGTYSVLNPELSYQLRDRKLGALGEAFTEQPPAAILSANMGCITHLQSGTGIPVK
HWVEVLDAALLA

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=413, Percent_Identity=57.8692493946731, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1788576, Length=354, Percent_Identity=22.5988700564972, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786497, Length=249, Percent_Identity=25.7028112449799, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: 1.1.3.15

Molecular weight: Translated: 44860; Mature: 44860

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTQLAPQYQGTPEGQEAEAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLE
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GAEPTRKTQLHLDRCLTCRNCESTCPSGVQYGHLVDIGRRIVEEKVPRPRGEAAARWMLK
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
EGLPSPLFAPAMKVGQAVRGLLPERLRAKVPPRQDAGAWPTRSHARKVLMLEGCVQPSMA
HCCCCCHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
PNINNATARVLDAAGIQTVVAREAGCCGAVKFHLNDQEGGLAQMRANIDAWWPYVERGEV
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCCHHHCCCCCCCCE
EAIVMNASGCGVTVKEYGHLLRNDAQYAEKAARVSALTRDLSELLPDLVERLRGRIQPPA
EEEEEECCCCCEEHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
APLAYHPPCTLQHGQKLRGGVERHLAALGFELRVARTESHLCCGSAGTYSVLNPELSYQL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHEEEECCCCCEEECCCCCCEEECCCHHHHH
RDRKLGALGEAFTEQPPAAILSANMGCITHLQSGTGIPVKHWVEVLDAALLA
HHHHHHHHHHHHHCCCCHHHHHCCCHHHHEECCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTQLAPQYQGTPEGQEAEAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLE
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GAEPTRKTQLHLDRCLTCRNCESTCPSGVQYGHLVDIGRRIVEEKVPRPRGEAAARWMLK
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
EGLPSPLFAPAMKVGQAVRGLLPERLRAKVPPRQDAGAWPTRSHARKVLMLEGCVQPSMA
HCCCCCHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
PNINNATARVLDAAGIQTVVAREAGCCGAVKFHLNDQEGGLAQMRANIDAWWPYVERGEV
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCCHHHCCCCCCCCE
EAIVMNASGCGVTVKEYGHLLRNDAQYAEKAARVSALTRDLSELLPDLVERLRGRIQPPA
EEEEEECCCCCEEHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
APLAYHPPCTLQHGQKLRGGVERHLAALGFELRVARTESHLCCGSAGTYSVLNPELSYQL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHEEEECCCCCEEECCCCCCEEECCCHHHHH
RDRKLGALGEAFTEQPPAAILSANMGCITHLQSGTGIPVKHWVEVLDAALLA
HHHHHHHHHHHHHCCCCHHHHHCCCHHHHEECCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]