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The map label for this gene is amtB [H]

Identifier: 222109403

GI number: 222109403

Start: 204561

End: 205982

Strand: Direct

Name: amtB [H]

Synonym: Dtpsy_0182

Alternate gene names: 222109403

Gene position: 204561-205982 (Clockwise)

Preceding gene: 222109402

Following gene: 222109404

Centisome position: 5.39

GC content: 68.78

Gene sequence:

>1422_bases
ATGAAAAAACTATTTGCTTCCCTACTGATCGGTGCAAGCCTGCTGGCCGGTGGCGCCGGCGCGCTGGCCCAGACACCCGA
CGCGGCCGCGGCGGCTTCGGAGCCCGTGGCCGTCGCCCCCGCGCCCGCTGCCGAGCCCGTCGCAGCACCTGCTGCGGCAC
CCGCACCCGCCCCCGCGGCAGCGGCGCCCGCCGCGCCCAAGGTGGACTCGGGCGACACCGCCTGGATGCTGACCTCCACG
CTGCTCGTGATCCTGATGATCATCCCGGGCCTGGCCCTGTTCTACGGGGGGCTGGGCCGCACCAAGAACATGCTGTCCGT
GCTGATGCAGGTGTTCGTGATCTTTGCCCTGGTGTCGCTGCTGTGGTCCATCTATGGCTACAGCCTCGCGTTCTCCGGCG
AGGGCCAATTCGTGGGCAACCTGTCCAAGCTTTTCCTGAAGGGCGTGAGCCAGGAAACCTTTGGTGCGCTGGCGACCATT
CCCGAATACGTGTTCATCGCCTTCCAGGGCACGTTCGCGGCCATCACCGTGGCGCTGATCGTGGGCTCCTTTGCGGAACG
CATGCGTTTTGCGGCCGTGCTCATCTTCGCGCTGCTGTGGTTCACCTTCAGCTACGTGCCCATGGCCCACATCGTGTGGG
GTGGCGGCCTGCTGGCGGCCGACGGCGCGCTCGATTTTGCGGGCGGCACCGTGGTGCACATCAATGCCGGTATCGCGGGC
CTGGTGGGTGCCTACATGGTGGGCAAGCGCATCGGCTTCGGCAAAGAGGCCTTCACCCCGCACAGCCTGACGCTGACCAT
GGTGGGCGCCTCGCTGCTGTGGGTGGGCTGGTTCGGCTTCAACGCCGGCTCTGCAGGTGCCGCCAACGCCCTTGCGGGCC
TGGCCTTCGTGAACACGGTGCTGGCCACCTCGGCCGCCACGCTGTCGTGGATCGCCGGCGAAGCGCTGCACAAGGGCAAG
GCCTCCATGCTGGGCGCGGCGTCGGGCGCGGTGGCCGGCCTGGTGGCGGTCACGCCCGCCGCGGGCTTCGTGGGCCCCAT
GGGCTCCATCGCGATCGGCCTGATCGCCGGCCTGGTCTGCCTGTGGGGTGTGGGCGGCCTGAAGAAGATGCTGAACGTGG
ACGACGCCTTCGACGTGTTCGGCGTGCATGGCGTGGGCGGCATCGTGGGCGCCATCCTCACCGGCGTGTTCGCGGCCCAG
GGCCTGGGCGGCACCGGCGGCCTGACGCCCGACACCTTCAGCATGGGCGCGCAGGTGTGGGTGCAGGTCAAGAGCGTGCT
GTTCACCATCGTGTGGTCGGGCGTGGTGTCCTTCATCGCCTACAAGGTCGCCGACCTGCTGGTGGGCCTGCGCGTGAGCG
AGGAAGCCGAGCGCGAGGGCCTGGACATCACCTCCCACGGCGAGACGGCCTACCACCGCTGA

Upstream 100 bases:

>100_bases
CTGGAGCAGGTGGTGCGCATCCGCACCGGCGAGACCGGCCAGGAAGCCCTGTGAGCGCGCGCCGCACAGGCATTCGTCCC
TCCCGACCCAGAGATCTGCC

Downstream 100 bases:

>100_bases
GCGGCGTTGAACGGCGGGCGCCGCGTGGTGGCGCGGCGCCCCCGATGACAAGCGAGCAACTCCCTGTCCCTTTTTTGGGC
CCGCCCTTGGCGGGCCTTTT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MKKLFASLLIGASLLAGGAGALAQTPDAAAAASEPVAVAPAPAAEPVAAPAAAPAPAPAAAAPAAPKVDSGDTAWMLTST
LLVILMIIPGLALFYGGLGRTKNMLSVLMQVFVIFALVSLLWSIYGYSLAFSGEGQFVGNLSKLFLKGVSQETFGALATI
PEYVFIAFQGTFAAITVALIVGSFAERMRFAAVLIFALLWFTFSYVPMAHIVWGGGLLAADGALDFAGGTVVHINAGIAG
LVGAYMVGKRIGFGKEAFTPHSLTLTMVGASLLWVGWFGFNAGSAGAANALAGLAFVNTVLATSAATLSWIAGEALHKGK
ASMLGAASGAVAGLVAVTPAAGFVGPMGSIAIGLIAGLVCLWGVGGLKKMLNVDDAFDVFGVHGVGGIVGAILTGVFAAQ
GLGGTGGLTPDTFSMGAQVWVQVKSVLFTIVWSGVVSFIAYKVADLLVGLRVSEEAEREGLDITSHGETAYHR

Sequences:

>Translated_473_residues
MKKLFASLLIGASLLAGGAGALAQTPDAAAAASEPVAVAPAPAAEPVAAPAAAPAPAPAAAAPAAPKVDSGDTAWMLTST
LLVILMIIPGLALFYGGLGRTKNMLSVLMQVFVIFALVSLLWSIYGYSLAFSGEGQFVGNLSKLFLKGVSQETFGALATI
PEYVFIAFQGTFAAITVALIVGSFAERMRFAAVLIFALLWFTFSYVPMAHIVWGGGLLAADGALDFAGGTVVHINAGIAG
LVGAYMVGKRIGFGKEAFTPHSLTLTMVGASLLWVGWFGFNAGSAGAANALAGLAFVNTVLATSAATLSWIAGEALHKGK
ASMLGAASGAVAGLVAVTPAAGFVGPMGSIAIGLIAGLVCLWGVGGLKKMLNVDDAFDVFGVHGVGGIVGAILTGVFAAQ
GLGGTGGLTPDTFSMGAQVWVQVKSVLFTIVWSGVVSFIAYKVADLLVGLRVSEEAEREGLDITSHGETAYHR
>Mature_473_residues
MKKLFASLLIGASLLAGGAGALAQTPDAAAAASEPVAVAPAPAAEPVAAPAAAPAPAPAAAAPAAPKVDSGDTAWMLTST
LLVILMIIPGLALFYGGLGRTKNMLSVLMQVFVIFALVSLLWSIYGYSLAFSGEGQFVGNLSKLFLKGVSQETFGALATI
PEYVFIAFQGTFAAITVALIVGSFAERMRFAAVLIFALLWFTFSYVPMAHIVWGGGLLAADGALDFAGGTVVHINAGIAG
LVGAYMVGKRIGFGKEAFTPHSLTLTMVGASLLWVGWFGFNAGSAGAANALAGLAFVNTVLATSAATLSWIAGEALHKGK
ASMLGAASGAVAGLVAVTPAAGFVGPMGSIAIGLIAGLVCLWGVGGLKKMLNVDDAFDVFGVHGVGGIVGAILTGVFAAQ
GLGGTGGLTPDTFSMGAQVWVQVKSVLFTIVWSGVVSFIAYKVADLLVGLRVSEEAEREGLDITSHGETAYHR

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Homo sapiens, GI156627565, Length=155, Percent_Identity=32.9032258064516, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI1786656, Length=417, Percent_Identity=61.6306954436451, Blast_Score=500, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17550182, Length=431, Percent_Identity=29.9303944315545, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17550180, Length=437, Percent_Identity=29.5194508009153, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32564042, Length=440, Percent_Identity=26.5909090909091, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI133931100, Length=426, Percent_Identity=26.7605633802817, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324187, Length=407, Percent_Identity=35.3808353808354, Blast_Score=225, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6325396, Length=419, Percent_Identity=35.3221957040573, Blast_Score=216, Evalue=8e-57,
Organism=Saccharomyces cerevisiae, GI6321559, Length=415, Percent_Identity=33.4939759036145, Blast_Score=209, Evalue=6e-55,
Organism=Drosophila melanogaster, GI161078314, Length=436, Percent_Identity=27.9816513761468, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 47813; Mature: 47813

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLFASLLIGASLLAGGAGALAQTPDAAAAASEPVAVAPAPAAEPVAAPAAAPAPAPAA
CHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCC
AAPAAPKVDSGDTAWMLTSTLLVILMIIPGLALFYGGLGRTKNMLSVLMQVFVIFALVSL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LWSIYGYSLAFSGEGQFVGNLSKLFLKGVSQETFGALATIPEYVFIAFQGTFAAITVALI
HHHHHCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEECCHHHHHHHHHH
VGSFAERMRFAAVLIFALLWFTFSYVPMAHIVWGGGLLAADGALDFAGGTVVHINAGIAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEECCCCEEEEECCCHHH
LVGAYMVGKRIGFGKEAFTPHSLTLTMVGASLLWVGWFGFNAGSAGAANALAGLAFVNTV
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LATSAATLSWIAGEALHKGKASMLGAASGAVAGLVAVTPAAGFVGPMGSIAIGLIAGLVC
HHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
LWGVGGLKKMLNVDDAFDVFGVHGVGGIVGAILTGVFAAQGLGGTGGLTPDTFSMGAQVW
HHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
VQVKSVLFTIVWSGVVSFIAYKVADLLVGLRVSEEAEREGLDITSHGETAYHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKLFASLLIGASLLAGGAGALAQTPDAAAAASEPVAVAPAPAAEPVAAPAAAPAPAPAA
CHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCC
AAPAAPKVDSGDTAWMLTSTLLVILMIIPGLALFYGGLGRTKNMLSVLMQVFVIFALVSL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LWSIYGYSLAFSGEGQFVGNLSKLFLKGVSQETFGALATIPEYVFIAFQGTFAAITVALI
HHHHHCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEECCHHHHHHHHHH
VGSFAERMRFAAVLIFALLWFTFSYVPMAHIVWGGGLLAADGALDFAGGTVVHINAGIAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEECCCCEEEEECCCHHH
LVGAYMVGKRIGFGKEAFTPHSLTLTMVGASLLWVGWFGFNAGSAGAANALAGLAFVNTV
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LATSAATLSWIAGEALHKGKASMLGAASGAVAGLVAVTPAAGFVGPMGSIAIGLIAGLVC
HHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
LWGVGGLKKMLNVDDAFDVFGVHGVGGIVGAILTGVFAAQGLGGTGGLTPDTFSMGAQVW
HHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
VQVKSVLFTIVWSGVVSFIAYKVADLLVGLRVSEEAEREGLDITSHGETAYHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]