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The map label for this gene is caiD [H]

Identifier: 222109346

GI number: 222109346

Start: 140970

End: 141734

Strand: Direct

Name: caiD [H]

Synonym: Dtpsy_0124

Alternate gene names: 222109346

Gene position: 140970-141734 (Clockwise)

Preceding gene: 222109345

Following gene: 222109347

Centisome position: 3.71

GC content: 71.5

Gene sequence:

>765_bases
ATGGCATCCACGGTACTGGTCGAAGTGCGCGGCAACGTGCTGATCATGACGCTGAGCAACCCCGAGGCGCGCAATGCCGC
CACGCTGGAGATGGCCGAGGCCATGGCGGCAGCGCTGGACGAGCTGGACCGCAACCCCGCCCTGCAGGTGGGCGTCATCA
CCGGCGCGGGCGGCACCTTCTGCGCGGGCATGGACCTGAAGGGCTTTTTGCAGGGCAAGCGCCCCAGCCTGCCGGGGCGG
GGGTTCCTGGGCCTCACGCAGCAGCCGCCGCGCAAGCCGCTGATTGCGGCCGTGGAAGGCTACGCCCTCGCCGGCGGCTT
TGAAACCGTGCTGGCCTGCGACCTGATCGTCGCCGCCAAGACGGCCCAATTCGGCCTTCCCGAGGTCAAGCGTGGCTTGG
CCGCCGCGGCTGGCGGGCTGCTGCGCCTGCCCAGGCGCCTGCCCTACCACCTGGCGATGGAAGCCATCCTCACGGGCGAC
ATGTTCAGCGCCGAGCGCGCGCAGGCCCATGGCCTGGTCAACCGCCTGGTTGAGCCCGGCCAGGCGCTGGACAGCGCCCT
GGAACTGGCCCAGGCTGTGGCCGCCAACGGCCCGCTCGCGCTGATCGCCAGCAAGCGCGTGGCGCAGGAGTCGGCCGACT
GGACGCAGGCCGAGATGTTCGAGCGCCAGGCCGTCATCACCGCGCCGGTGTTCGCCTCGCTGGATGCGCGCGAGGGCGCC
GCGGCCTTTGCCGAGAAGCGCCCGCCGGTCTGGAAGGGCGTTTGA

Upstream 100 bases:

>100_bases
CTGCGGGGCGGCCCTTGCGCGGCCGCCCTGGTCTCAGGCAGCGCCAGTTTCATAGGTCGCGGGCCGCGTAACGCGGCGCA
GAACTGCAAGGACTTTTTTT

Downstream 100 bases:

>100_bases
TCGGAAAAGGGGCGCTAACGCTCACCTATCAGGCGCCATTAGCTAGTTAATTCATAGCAAAAGCGGCCGCAGTGCCGCAA
GGACCCCAGGAGACACAACC

Product: enoyl-CoA hydratase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MASTVLVEVRGNVLIMTLSNPEARNAATLEMAEAMAAALDELDRNPALQVGVITGAGGTFCAGMDLKGFLQGKRPSLPGR
GFLGLTQQPPRKPLIAAVEGYALAGGFETVLACDLIVAAKTAQFGLPEVKRGLAAAAGGLLRLPRRLPYHLAMEAILTGD
MFSAERAQAHGLVNRLVEPGQALDSALELAQAVAANGPLALIASKRVAQESADWTQAEMFERQAVITAPVFASLDAREGA
AAFAEKRPPVWKGV

Sequences:

>Translated_254_residues
MASTVLVEVRGNVLIMTLSNPEARNAATLEMAEAMAAALDELDRNPALQVGVITGAGGTFCAGMDLKGFLQGKRPSLPGR
GFLGLTQQPPRKPLIAAVEGYALAGGFETVLACDLIVAAKTAQFGLPEVKRGLAAAAGGLLRLPRRLPYHLAMEAILTGD
MFSAERAQAHGLVNRLVEPGQALDSALELAQAVAANGPLALIASKRVAQESADWTQAEMFERQAVITAPVFASLDAREGA
AAFAEKRPPVWKGV
>Mature_253_residues
ASTVLVEVRGNVLIMTLSNPEARNAATLEMAEAMAAALDELDRNPALQVGVITGAGGTFCAGMDLKGFLQGKRPSLPGRG
FLGLTQQPPRKPLIAAVEGYALAGGFETVLACDLIVAAKTAQFGLPEVKRGLAAAAGGLLRLPRRLPYHLAMEAILTGDM
FSAERAQAHGLVNRLVEPGQALDSALELAQAVAANGPLALIASKRVAQESADWTQAEMFERQAVITAPVFASLDAREGAA
AFAEKRPPVWKGV

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=255, Percent_Identity=33.7254901960784, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI31542718, Length=249, Percent_Identity=30.9236947791165, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI70995211, Length=253, Percent_Identity=31.2252964426877, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=30.46875, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI20127408, Length=184, Percent_Identity=29.8913043478261, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI68989263, Length=187, Percent_Identity=33.1550802139037, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI150378541, Length=243, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI221142681, Length=262, Percent_Identity=41.6030534351145, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI1787659, Length=260, Percent_Identity=36.9230769230769, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI87082183, Length=238, Percent_Identity=32.7731092436975, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=30.0380228136882, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788682, Length=202, Percent_Identity=32.1782178217822, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788597, Length=242, Percent_Identity=28.5123966942149, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790281, Length=115, Percent_Identity=40.8695652173913, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=34.8178137651822, Blast_Score=141, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI25145438, Length=242, Percent_Identity=34.297520661157, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17560910, Length=254, Percent_Identity=30.3149606299213, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17540714, Length=236, Percent_Identity=30.5084745762712, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=268, Percent_Identity=25.7462686567164, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=252, Percent_Identity=26.5873015873016, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=244, Percent_Identity=27.8688524590164, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17558304, Length=189, Percent_Identity=30.6878306878307, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17508951, Length=244, Percent_Identity=28.2786885245902, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=244, Percent_Identity=28.2786885245902, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=244, Percent_Identity=28.2786885245902, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=218, Percent_Identity=25.2293577981651, Blast_Score=64, Evalue=1e-10,
Organism=Drosophila melanogaster, GI20129971, Length=251, Percent_Identity=34.6613545816733, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24653477, Length=251, Percent_Identity=34.6613545816733, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=35.3383458646617, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24650670, Length=217, Percent_Identity=34.5622119815668, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21357171, Length=256, Percent_Identity=29.6875, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19922422, Length=255, Percent_Identity=29.8039215686275, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19921000, Length=182, Percent_Identity=33.5164835164835, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583077, Length=182, Percent_Identity=33.5164835164835, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583079, Length=182, Percent_Identity=33.5164835164835, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920382, Length=260, Percent_Identity=27.6923076923077, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 26550; Mature: 26419

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASTVLVEVRGNVLIMTLSNPEARNAATLEMAEAMAAALDELDRNPALQVGVITGAGGTF
CCCEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCE
CAGMDLKGFLQGKRPSLPGRGFLGLTQQPPRKPLIAAVEGYALAGGFETVLACDLIVAAK
EECCCHHHHHCCCCCCCCCCCEECCCCCCCCCCHHHHHCCEEECCCHHHHHHHHHHHHHH
TAQFGLPEVKRGLAAAAGGLLRLPRRLPYHLAMEAILTGDMFSAERAQAHGLVNRLVEPG
HHHCCCHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCH
QALDSALELAQAVAANGPLALIASKRVAQESADWTQAEMFERQAVITAPVFASLDAREGA
HHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCHHHHHHHHHHHHEECHHHHCCCCCCCH
AAFAEKRPPVWKGV
HHHHHCCCCCCCCC
>Mature Secondary Structure 
ASTVLVEVRGNVLIMTLSNPEARNAATLEMAEAMAAALDELDRNPALQVGVITGAGGTF
CCEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCE
CAGMDLKGFLQGKRPSLPGRGFLGLTQQPPRKPLIAAVEGYALAGGFETVLACDLIVAAK
EECCCHHHHHCCCCCCCCCCCEECCCCCCCCCCHHHHHCCEEECCCHHHHHHHHHHHHHH
TAQFGLPEVKRGLAAAAGGLLRLPRRLPYHLAMEAILTGDMFSAERAQAHGLVNRLVEPG
HHHCCCHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCH
QALDSALELAQAVAANGPLALIASKRVAQESADWTQAEMFERQAVITAPVFASLDAREGA
HHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCHHHHHHHHHHHHEECHHHHCCCCCCCH
AAFAEKRPPVWKGV
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA