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The map label for this gene is minD [H]

Identifier: 222109308

GI number: 222109308

Start: 94090

End: 94905

Strand: Direct

Name: minD [H]

Synonym: Dtpsy_0085

Alternate gene names: 222109308

Gene position: 94090-94905 (Clockwise)

Preceding gene: 222109307

Following gene: 222109309

Centisome position: 2.48

GC content: 64.83

Gene sequence:

>816_bases
ATGGCCAAAATCGTCGTCGTGACCTCCGGCAAGGGGGGCGTGGGCAAGACCACCACCAGTGCCGCCTTCGCATCGGGCCT
GGCGCTCGCCGGCCACAAGACCGCCGTCATCGACTTTGACGTCGGCCTGCGCAACCTGGACCTCATCATGGGCTGCGAGC
GCCGCGTGGTGTATGACCTGATCAACGTGATCCAGGGCGAAGCCAACCTGAACCAGGCCCTCATCAAGGACAAGCAGTGC
GAGAACCTGTTCGTGCTGGCCGCCAGCCAGACGCGCGACAAGGACGCGCTCACGCAGGACGGCGTGGGCAAGGTGCTCAA
GGACCTCGCCGACATGGGCTTTGACTACATCGTCTGCGACTCGCCCGCCGGCATCGAAAGCGGTGCGCTGATGGCCATGC
ACTTCGCCGACGAGGCGCTGCTGGTCACCAACCCCGAAGTCTCCAGCGTGCGCGACTCCGACCGCATCCTGGGCATGCTC
AGCAGCAAGACCAAGCGCGCCATCGAAGGCGGCGAGCCCGTCAAGGAACACCTGCTGATCACGCGCTACAACCCCCATCG
TGTGGAAGACGGCCAGATGCTGAGCCTGGAGGACATCCAAGACATCCTGCGCATCAAGCTCATCGGCGTGATCCCCGAGT
CCGAGAGCGTGCTGCAGGCCTCCAACCAGGGCCTGCCCGCCATCCACCTGTCGGGCACCGATGTGTCCGAGGCCTACAAG
GACGTGGTTGCGCGCTTCCTGGGCGAGGACAAGCCCCTGCGTTTCATCGAGGCGGCCAAGCCCGGCTTCTTCAAGCGCAT
CTTCGGCGGGAGGTAA

Upstream 100 bases:

>100_bases
CCCAGGTGCGGCTGGACGGTGAGAAGCTGCTCATCGAGCCGCTGTGACTGTCCCCACCCTTTCTTACTCCACAGACCGCA
ACGCAAGGACCTCTCAGAAC

Downstream 100 bases:

>100_bases
GCGAGATGTCCCTGCTGTCCTTCCTGCTCGGCGAGAAGAAAAAGACGGCCTCGGTGGCCAAGGAGCGCCTGCAGATCATC
CTGGCGCACGAGCGCAGCGG

Product: septum site-determining protein mind

Products: NA

Alternate protein names: Cell division inhibitor minD [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MAKIVVVTSGKGGVGKTTTSAAFASGLALAGHKTAVIDFDVGLRNLDLIMGCERRVVYDLINVIQGEANLNQALIKDKQC
ENLFVLAASQTRDKDALTQDGVGKVLKDLADMGFDYIVCDSPAGIESGALMAMHFADEALLVTNPEVSSVRDSDRILGML
SSKTKRAIEGGEPVKEHLLITRYNPHRVEDGQMLSLEDIQDILRIKLIGVIPESESVLQASNQGLPAIHLSGTDVSEAYK
DVVARFLGEDKPLRFIEAAKPGFFKRIFGGR

Sequences:

>Translated_271_residues
MAKIVVVTSGKGGVGKTTTSAAFASGLALAGHKTAVIDFDVGLRNLDLIMGCERRVVYDLINVIQGEANLNQALIKDKQC
ENLFVLAASQTRDKDALTQDGVGKVLKDLADMGFDYIVCDSPAGIESGALMAMHFADEALLVTNPEVSSVRDSDRILGML
SSKTKRAIEGGEPVKEHLLITRYNPHRVEDGQMLSLEDIQDILRIKLIGVIPESESVLQASNQGLPAIHLSGTDVSEAYK
DVVARFLGEDKPLRFIEAAKPGFFKRIFGGR
>Mature_270_residues
AKIVVVTSGKGGVGKTTTSAAFASGLALAGHKTAVIDFDVGLRNLDLIMGCERRVVYDLINVIQGEANLNQALIKDKQCE
NLFVLAASQTRDKDALTQDGVGKVLKDLADMGFDYIVCDSPAGIESGALMAMHFADEALLVTNPEVSSVRDSDRILGMLS
SKTKRAIEGGEPVKEHLLITRYNPHRVEDGQMLSLEDIQDILRIKLIGVIPESESVLQASNQGLPAIHLSGTDVSEAYKD
VVARFLGEDKPLRFIEAAKPGFFKRIFGGR

Specific function: ATPase required for the correct placement of the division site. Cell division inhibitors minC and minD act in concert to form an inhibitor capable of blocking formation of the polar Z ring septums. Rapidly oscillates between the poles of the cell to desta

COG id: COG2894

COG function: function code D; Septum formation inhibitor-activating ATPase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the parA family. MinD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787423, Length=270, Percent_Identity=71.4814814814815, Blast_Score=404, Evalue=1e-114,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR010223 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: NA

Molecular weight: Translated: 29255; Mature: 29124

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIVVVTSGKGGVGKTTTSAAFASGLALAGHKTAVIDFDVGLRNLDLIMGCERRVVYDL
CEEEEEEECCCCCCCCCHHHHHHHHCEEECCCCEEEEEECCCCCHHHHHHCCCHHHHHHH
INVIQGEANLNQALIKDKQCENLFVLAASQTRDKDALTQDGVGKVLKDLADMGFDYIVCD
HHHHCCCCCCHHHHHCCCCCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHCCCCEEEEC
SPAGIESGALMAMHFADEALLVTNPEVSSVRDSDRILGMLSSKTKRAIEGGEPVKEHLLI
CCCCCCCCCEEEEEECCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHCEEE
TRYNPHRVEDGQMLSLEDIQDILRIKLIGVIPESESVLQASNQGLPAIHLSGTDVSEAYK
EECCCCCCCCCCEECHHHHHHHHHEEEEEECCCCHHHHHHCCCCCCEEEECCCCHHHHHH
DVVARFLGEDKPLRFIEAAKPGFFKRIFGGR
HHHHHHHCCCCCEEHHHHCCCCHHHHHCCCC
>Mature Secondary Structure 
AKIVVVTSGKGGVGKTTTSAAFASGLALAGHKTAVIDFDVGLRNLDLIMGCERRVVYDL
EEEEEEECCCCCCCCCHHHHHHHHCEEECCCCEEEEEECCCCCHHHHHHCCCHHHHHHH
INVIQGEANLNQALIKDKQCENLFVLAASQTRDKDALTQDGVGKVLKDLADMGFDYIVCD
HHHHCCCCCCHHHHHCCCCCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHCCCCEEEEC
SPAGIESGALMAMHFADEALLVTNPEVSSVRDSDRILGMLSSKTKRAIEGGEPVKEHLLI
CCCCCCCCCEEEEEECCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHCEEE
TRYNPHRVEDGQMLSLEDIQDILRIKLIGVIPESESVLQASNQGLPAIHLSGTDVSEAYK
EECCCCCCCCCCEECHHHHHHHHHEEEEEECCCCHHHHHHCCCCCCEEEECCCCHHHHHH
DVVARFLGEDKPLRFIEAAKPGFFKRIFGGR
HHHHHHHCCCCCEEHHHHCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]