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The map label for this gene is purA [H]

Identifier: 222102825

GI number: 222102825

Start: 497289

End: 498467

Strand: Reverse

Name: purA [H]

Synonym: Avi_7550

Alternate gene names: 222102825

Gene position: 498467-497289 (Counterclockwise)

Preceding gene: 222102826

Following gene: 222102824

Centisome position: 78.9

GC content: 57.0

Gene sequence:

>1179_bases
ATGAGCACCGCTTATGCAGTTCTCGGCGCTGGTTGGGGCGATGAGGGCAAGGGCTTGATGACTGATGCCCTGGCCAGCGC
TGGAAGCGTTGTCGTGCGGGCCAATGGTGGCGCCCAAGCTGGTCATACGGTCCAGCGTCCGGATGGCACACGCCATGTGT
TCCATCATGTAGGCAGCGGTGCCCTTCGGGGTGCCACTACCTACATGTCGCGCTTCTTTATTCATAACCCCCTGCTGCTG
CTGCGGGAGTTGTCTGAACTCGACAAGATTGGCGTTCGCCCAAGCTTGTTTGCCGACCCAAACGGGCTGGTGACCACTCC
GTGGGATATGATGATCAACCAGATCGCAGAGGAGGCTCGCAATAGCGGGCGACATGGCTCCTGCGGCCTTGGAATCAATG
AGACCATCCGACGGAGCGAAAGAGATGGATTCAAGCTTCAGTTCAGCGATCTGGCAAACCTCACAAAGATGGATGAAATC
GTCAGACGGATCCAGAAGGAGTGGGTGCCTTCTCGATTAACGGAGCTAGGATTTTTGCCATCTGAGGCCTGGCAAGCGCG
GCTTGTTTCTCCGGTTATCGCGGAGCGATTTACACAGGACGTTGAAGATTTCAGTCGTGTCGTCTGGCCCAATGATGGTA
CGGTCGCAGCAGTGGCGTCTGCAAGCGACAGGCTCATTTTCGAGGGCGCCCAAGGCTTGTTACTCGATCAGGATCATGCC
TTCTTTCCGCATGTCACTCCCTCCAAGACCGGAATTGCCAATCCATCTATCCTTGCGGCGGAATGGGGGGTTGGTGAAAT
CAAGGCAATCTACATGACACGCGCTTACGCCACACGGCATGGCGCTGGTCCATTTCCACGGGCTACGCCCGAGATGGTCT
ACCCAGATGCAACGAATATCCCTAACCTTTGGCAGGGCACGTTGAGGTTCGGAGCGCTCGATCTCGATCTGCTGGCCAAA
ACCATTCTTGAGGACGAAGCTCGGTCTTCAGTGCGGGTCGAGACGTCTTTCGCAATGACGTGCCTCGATCAGATCGCCGA
TCAGGCGGTCTGGTGGCGGAGCAATATTCGGGAAACCGGCGCTGGCAAAGATCTGCTGGAGGCAGCACGTCGTCATTTGC
AATGTCCGCTCGCAGGTAAAAGCTTCGGCCCCTGCCGTGAGGACGTCACGTTCGGCTAG

Upstream 100 bases:

>100_bases
CGAGTTGGAGCGGCGATACAGCTTTGGTCGTCCAGAAGGCGACGAAAGGTCTTTCGCCTGCGACCAAAAGCGGAGGCCTG
CGTTGGTTCGGCGGTAAGAA

Downstream 100 bases:

>100_bases
GTTCCGATCTGACCGTGATCCACTGTATCTCCGACATCGCTCTCCCGCTGGAGTCACTGAAATCTGTTTTCCTTGTTATG
CCGGTTCTGGCATTTCTCTG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSTAYAVLGAGWGDEGKGLMTDALASAGSVVVRANGGAQAGHTVQRPDGTRHVFHHVGSGALRGATTYMSRFFIHNPLLL
LRELSELDKIGVRPSLFADPNGLVTTPWDMMINQIAEEARNSGRHGSCGLGINETIRRSERDGFKLQFSDLANLTKMDEI
VRRIQKEWVPSRLTELGFLPSEAWQARLVSPVIAERFTQDVEDFSRVVWPNDGTVAAVASASDRLIFEGAQGLLLDQDHA
FFPHVTPSKTGIANPSILAAEWGVGEIKAIYMTRAYATRHGAGPFPRATPEMVYPDATNIPNLWQGTLRFGALDLDLLAK
TILEDEARSSVRVETSFAMTCLDQIADQAVWWRSNIRETGAGKDLLEAARRHLQCPLAGKSFGPCREDVTFG

Sequences:

>Translated_392_residues
MSTAYAVLGAGWGDEGKGLMTDALASAGSVVVRANGGAQAGHTVQRPDGTRHVFHHVGSGALRGATTYMSRFFIHNPLLL
LRELSELDKIGVRPSLFADPNGLVTTPWDMMINQIAEEARNSGRHGSCGLGINETIRRSERDGFKLQFSDLANLTKMDEI
VRRIQKEWVPSRLTELGFLPSEAWQARLVSPVIAERFTQDVEDFSRVVWPNDGTVAAVASASDRLIFEGAQGLLLDQDHA
FFPHVTPSKTGIANPSILAAEWGVGEIKAIYMTRAYATRHGAGPFPRATPEMVYPDATNIPNLWQGTLRFGALDLDLLAK
TILEDEARSSVRVETSFAMTCLDQIADQAVWWRSNIRETGAGKDLLEAARRHLQCPLAGKSFGPCREDVTFG
>Mature_391_residues
STAYAVLGAGWGDEGKGLMTDALASAGSVVVRANGGAQAGHTVQRPDGTRHVFHHVGSGALRGATTYMSRFFIHNPLLLL
RELSELDKIGVRPSLFADPNGLVTTPWDMMINQIAEEARNSGRHGSCGLGINETIRRSERDGFKLQFSDLANLTKMDEIV
RRIQKEWVPSRLTELGFLPSEAWQARLVSPVIAERFTQDVEDFSRVVWPNDGTVAAVASASDRLIFEGAQGLLLDQDHAF
FPHVTPSKTGIANPSILAAEWGVGEIKAIYMTRAYATRHGAGPFPRATPEMVYPDATNIPNLWQGTLRFGALDLDLLAKT
ILEDEARSSVRVETSFAMTCLDQIADQAVWWRSNIRETGAGKDLLEAARRHLQCPLAGKSFGPCREDVTFG

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790620, Length=296, Percent_Identity=32.0945945945946, Blast_Score=108, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6324109, Length=299, Percent_Identity=28.4280936454849, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 42871; Mature: 42740

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAYAVLGAGWGDEGKGLMTDALASAGSVVVRANGGAQAGHTVQRPDGTRHVFHHVGSG
CCCCEEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHCCCC
ALRGATTYMSRFFIHNPLLLLRELSELDKIGVRPSLFADPNGLVTTPWDMMINQIAEEAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEECCHHHHHHHHHHHHH
NSGRHGSCGLGINETIRRSERDGFKLQFSDLANLTKMDEIVRRIQKEWVPSRLTELGFLP
HCCCCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SEAWQARLVSPVIAERFTQDVEDFSRVVWPNDGTVAAVASASDRLIFEGAQGLLLDQDHA
CHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCEEEECCCCCEECCCCC
FFPHVTPSKTGIANPSILAAEWGVGEIKAIYMTRAYATRHGAGPFPRATPEMVYPDATNI
CCCCCCCCCCCCCCCCEEEECCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
PNLWQGTLRFGALDLDLLAKTILEDEARSSVRVETSFAMTCLDQIADQAVWWRSNIRETG
CHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHCHHCC
AGKDLLEAARRHLQCPLAGKSFGPCREDVTFG
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STAYAVLGAGWGDEGKGLMTDALASAGSVVVRANGGAQAGHTVQRPDGTRHVFHHVGSG
CCCEEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHCCCC
ALRGATTYMSRFFIHNPLLLLRELSELDKIGVRPSLFADPNGLVTTPWDMMINQIAEEAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEECCHHHHHHHHHHHHH
NSGRHGSCGLGINETIRRSERDGFKLQFSDLANLTKMDEIVRRIQKEWVPSRLTELGFLP
HCCCCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SEAWQARLVSPVIAERFTQDVEDFSRVVWPNDGTVAAVASASDRLIFEGAQGLLLDQDHA
CHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCEEEECCCCCEECCCCC
FFPHVTPSKTGIANPSILAAEWGVGEIKAIYMTRAYATRHGAGPFPRATPEMVYPDATNI
CCCCCCCCCCCCCCCCEEEECCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
PNLWQGTLRFGALDLDLLAKTILEDEARSSVRVETSFAMTCLDQIADQAVWWRSNIRETG
CHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHCHHCC
AGKDLLEAARRHLQCPLAGKSFGPCREDVTFG
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA