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The map label for this gene is nrdE [H]

Identifier: 222102711

GI number: 222102711

Start: 360696

End: 362777

Strand: Direct

Name: nrdE [H]

Synonym: Avi_7387

Alternate gene names: 222102711

Gene position: 360696-362777 (Clockwise)

Preceding gene: 222102710

Following gene: 222102712

Centisome position: 57.09

GC content: 54.85

Gene sequence:

>2082_bases
ATGTTAAACCTCTACGATGAGGATGGTAAAATCCAACTCGATAAGGACGCTCTGGCGGCAAAACAGTATTTCTTGCAGCA
CGTCAACCAGAACACCGTCTTCTTCCATAATCTACGGGAGAAGCTCGATTACCTCGTGGACGAAGGCTACTATGAGCAAG
CGGTGTTGGACCAGTATGCGTTCAACTTCGTCCGCGATCTTTTCGATCATGCCTATGTGAAGAAGTTTCGCTTTCCGACC
TTTCTCGGCGCCTTCAAATATTACACTAGCTACACGCTGAAGACCTTCGACGGAAAGCGCTATCTGGAGCGCTACGAAGA
CCGGATCTGCATGGTGGCATTGACGCTGGCGCAGGGCAATGAGGCTTTGGCGCGGGACATGGTCGATGAGATTATCTCCG
GTCGCTTCCAGCCGGCGACGCCGACCTTTCTCAATGCCGGCAAGAAGCAACGCGGCGAACTGGTCTCCTGCTTCCTGCTG
CGCATGGAAGACAATATGGAAAGCATCGCCCGTGGCATCAACTCGGCATTGCAACTTTCCAAGCGTGGGGGTGGGGTGGC
GTTATCCCTGACCAATATCCGCGAGGCTGGTGCACCGATCAAGCAGATCGAGAACCAGTCCTCGGGCGTCATCCCGGTGA
TGAAGTTACTGGAGGACAGTTTTTCCTATGCCAACCAGCTCGGTGCGCGCCAGGGGGCAGGGGCTGTCTACCTGAATGCC
CATCATCCCGATATCATGCGCTTCCTCGATACCAAGCGTGAAAACGCCGACGAGAAGATTAGGATCAAGACGCTGTCACT
CGGTGTGGTGATCCCCGACATCACCTTTGAATTGGCGAAGAACAATGAGGATATGTATCTGTTCTCACCCTACGACGTGG
AGCGTGTCTACGGCGTCGCTTTTTCCGAAATCTCAGTCACGGAAAAATACCGCGAGATGGTCGCCGACAGCCGCATTCGT
AAGAAGAAGATCAAGGCGCGTGATTTCTTCCAGGTGCTCGCCGAAATCCAGTTCGAGAGCGGCTATCCGTACATCATGTT
TGAGGACACGGTGAACCGCGCCAACCCGATAGCCGGACGCATTTCGATGAGCAATCTCTGCTCGGAAATCCTACAGGTGA
GCGACGTCAGCACTTATAATGACGACCTGTCCTACAAGACCATGGGTAAGGACATCTCCTGCAATCTCGGGTCCTTGAAC
ATCGCAGCCGTGATGGATTCCTCGGACTTCGGCAGGACCATTGAGACCGCGATCCGGGCGCTGACCGCCGTGTCCGACAT
GAGCCATATCTCTTCGGTTCCATCGATCGAGAAGGGAAATGATGACAGCCATGCGATCGGCCTTGGCCAGATGAACCTGC
ACGGCTATCTCGCACGCGAGCGCATCTTCTACGGATCGGAAGAGGGCATCGATTTCACCAACATGTATTTCTACACGGTG
ACCTATCACGCCATCCGTGCCTCGAACCGGATTGCTGCCGAGCGCGGCACGAGCTTCAAGGGCTTCGAGAACTCGAAATA
TGCATCCGGCGAGTATTTCGACAAGTATACGCAAACCGAATGGCTTCCAGCGACCAGGCGCGTCGCAGAACTATTCGAAA
CGGCCGGAATCTCAATCCCGACACAGGAAGACTGGTTGGCGCTGAAAGAGGTGGTGATGGCGTCCGGCCTTTATAACCAG
AACCTTCAGGCTGTGCCACCGACGGGGTCGATCTCCTACATCAACCATTCGACCTCCTCGATCCATCCGATCGTCTCGAA
GATCGAGATCCGCAAGGAAGGCAAGATCGGCCGCGTCTACTATCCGGCCGCCTTCATGAGCAATGATAACCTTGATTACT
ATCAGGACGCCTATGAGATCGGACCCGAGAAGATCATAGACACTTATGCGGCCGCCACCCAGCATGTGGATCAAGGTCTT
TCGCTGACGCTGTTCTTCCGTGACACCGCAACGACCCGCGACATCAATCGGGCACAGATCTACGCCTGGAAGAAGGGCAT
CAAGACCATCTATTACATTCGCCTTCGCCAGATGGCGCTGGCCGGCACGCAAGTGCAGGGCTGCGTATCCTGCGCGCTGT
GA

Upstream 100 bases:

>100_bases
CCAATGTCAAATATGGGATGGAACGATTTTGGATGTCACAACTTTCGAGCGCCCTTTGAAGGCGGACGAACAGGCGCTGG
ATTATCACGCGCTGAACGCG

Downstream 100 bases:

>100_bases
GATCGAGATATCACATGAACATACAGATGAAGACCAACACCCCGACGCAAACCAAGCCGGTGCGAGCGATCAACTGGAAC
CGCATCAACGACGACAAGGA

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit [H]

Number of amino acids: Translated: 693; Mature: 693

Protein sequence:

>693_residues
MLNLYDEDGKIQLDKDALAAKQYFLQHVNQNTVFFHNLREKLDYLVDEGYYEQAVLDQYAFNFVRDLFDHAYVKKFRFPT
FLGAFKYYTSYTLKTFDGKRYLERYEDRICMVALTLAQGNEALARDMVDEIISGRFQPATPTFLNAGKKQRGELVSCFLL
RMEDNMESIARGINSALQLSKRGGGVALSLTNIREAGAPIKQIENQSSGVIPVMKLLEDSFSYANQLGARQGAGAVYLNA
HHPDIMRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKNNEDMYLFSPYDVERVYGVAFSEISVTEKYREMVADSRIR
KKKIKARDFFQVLAEIQFESGYPYIMFEDTVNRANPIAGRISMSNLCSEILQVSDVSTYNDDLSYKTMGKDISCNLGSLN
IAAVMDSSDFGRTIETAIRALTAVSDMSHISSVPSIEKGNDDSHAIGLGQMNLHGYLARERIFYGSEEGIDFTNMYFYTV
TYHAIRASNRIAAERGTSFKGFENSKYASGEYFDKYTQTEWLPATRRVAELFETAGISIPTQEDWLALKEVVMASGLYNQ
NLQAVPPTGSISYINHSTSSIHPIVSKIEIRKEGKIGRVYYPAAFMSNDNLDYYQDAYEIGPEKIIDTYAAATQHVDQGL
SLTLFFRDTATTRDINRAQIYAWKKGIKTIYYIRLRQMALAGTQVQGCVSCAL

Sequences:

>Translated_693_residues
MLNLYDEDGKIQLDKDALAAKQYFLQHVNQNTVFFHNLREKLDYLVDEGYYEQAVLDQYAFNFVRDLFDHAYVKKFRFPT
FLGAFKYYTSYTLKTFDGKRYLERYEDRICMVALTLAQGNEALARDMVDEIISGRFQPATPTFLNAGKKQRGELVSCFLL
RMEDNMESIARGINSALQLSKRGGGVALSLTNIREAGAPIKQIENQSSGVIPVMKLLEDSFSYANQLGARQGAGAVYLNA
HHPDIMRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKNNEDMYLFSPYDVERVYGVAFSEISVTEKYREMVADSRIR
KKKIKARDFFQVLAEIQFESGYPYIMFEDTVNRANPIAGRISMSNLCSEILQVSDVSTYNDDLSYKTMGKDISCNLGSLN
IAAVMDSSDFGRTIETAIRALTAVSDMSHISSVPSIEKGNDDSHAIGLGQMNLHGYLARERIFYGSEEGIDFTNMYFYTV
TYHAIRASNRIAAERGTSFKGFENSKYASGEYFDKYTQTEWLPATRRVAELFETAGISIPTQEDWLALKEVVMASGLYNQ
NLQAVPPTGSISYINHSTSSIHPIVSKIEIRKEGKIGRVYYPAAFMSNDNLDYYQDAYEIGPEKIIDTYAAATQHVDQGL
SLTLFFRDTATTRDINRAQIYAWKKGIKTIYYIRLRQMALAGTQVQGCVSCAL
>Mature_693_residues
MLNLYDEDGKIQLDKDALAAKQYFLQHVNQNTVFFHNLREKLDYLVDEGYYEQAVLDQYAFNFVRDLFDHAYVKKFRFPT
FLGAFKYYTSYTLKTFDGKRYLERYEDRICMVALTLAQGNEALARDMVDEIISGRFQPATPTFLNAGKKQRGELVSCFLL
RMEDNMESIARGINSALQLSKRGGGVALSLTNIREAGAPIKQIENQSSGVIPVMKLLEDSFSYANQLGARQGAGAVYLNA
HHPDIMRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKNNEDMYLFSPYDVERVYGVAFSEISVTEKYREMVADSRIR
KKKIKARDFFQVLAEIQFESGYPYIMFEDTVNRANPIAGRISMSNLCSEILQVSDVSTYNDDLSYKTMGKDISCNLGSLN
IAAVMDSSDFGRTIETAIRALTAVSDMSHISSVPSIEKGNDDSHAIGLGQMNLHGYLARERIFYGSEEGIDFTNMYFYTV
TYHAIRASNRIAAERGTSFKGFENSKYASGEYFDKYTQTEWLPATRRVAELFETAGISIPTQEDWLALKEVVMASGLYNQ
NLQAVPPTGSISYINHSTSSIHPIVSKIEIRKEGKIGRVYYPAAFMSNDNLDYYQDAYEIGPEKIIDTYAAATQHVDQGL
SLTLFFRDTATTRDINRAQIYAWKKGIKTIYYIRLRQMALAGTQVQGCVSCAL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=635, Percent_Identity=26.2992125984252, Blast_Score=187, Evalue=3e-47,
Organism=Escherichia coli, GI1789030, Length=693, Percent_Identity=73.3044733044733, Blast_Score=1086, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=583, Percent_Identity=25.7289879931389, Blast_Score=173, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17555242, Length=642, Percent_Identity=26.1682242990654, Blast_Score=182, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6320914, Length=731, Percent_Identity=26.4021887824897, Blast_Score=183, Evalue=7e-47,
Organism=Saccharomyces cerevisiae, GI6322123, Length=729, Percent_Identity=25.9259259259259, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI17352481, Length=652, Percent_Identity=26.6871165644172, Blast_Score=173, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 78423; Mature: 78423

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLYDEDGKIQLDKDALAAKQYFLQHVNQNTVFFHNLREKLDYLVDEGYYEQAVLDQYA
CCCEECCCCCEEECHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHH
FNFVRDLFDHAYVKKFRFPTFLGAFKYYTSYTLKTFDGKRYLERYEDRICMVALTLAQGN
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEECCCHHHHHHHHCCEEEEEEEECCCC
EALARDMVDEIISGRFQPATPTFLNAGKKQRGELVSCFLLRMEDNMESIARGINSALQLS
HHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KRGGGVALSLTNIREAGAPIKQIENQSSGVIPVMKLLEDSFSYANQLGARQGAGAVYLNA
CCCCCEEEEECCHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
HHPDIMRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKNNEDMYLFSPYDVERVYGVA
CCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEECCCCCEEEECCCCHHHHHHHH
FSEISVTEKYREMVADSRIRKKKIKARDFFQVLAEIQFESGYPYIMFEDTVNRANPIAGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
ISMSNLCSEILQVSDVSTYNDDLSYKTMGKDISCNLGSLNIAAVMDSSDFGRTIETAIRA
CCHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHH
LTAVSDMSHISSVPSIEKGNDDSHAIGLGQMNLHGYLARERIFYGSEEGIDFTNMYFYTV
HHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHEEECCCCCCCEEEEEEEEE
TYHAIRASNRIAAERGTSFKGFENSKYASGEYFDKYTQTEWLPATRRVAELFETAGISIP
EEEHHHCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
TQEDWLALKEVVMASGLYNQNLQAVPPTGSISYINHSTSSIHPIVSKIEIRKEGKIGRVY
CCHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEE
YPAAFMSNDNLDYYQDAYEIGPEKIIDTYAAATQHVDQGLSLTLFFRDTATTRDINRAQI
ECEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHH
YAWKKGIKTIYYIRLRQMALAGTQVQGCVSCAL
HHHHHCCCEEEHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MLNLYDEDGKIQLDKDALAAKQYFLQHVNQNTVFFHNLREKLDYLVDEGYYEQAVLDQYA
CCCEECCCCCEEECHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHH
FNFVRDLFDHAYVKKFRFPTFLGAFKYYTSYTLKTFDGKRYLERYEDRICMVALTLAQGN
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEECCCHHHHHHHHCCEEEEEEEECCCC
EALARDMVDEIISGRFQPATPTFLNAGKKQRGELVSCFLLRMEDNMESIARGINSALQLS
HHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KRGGGVALSLTNIREAGAPIKQIENQSSGVIPVMKLLEDSFSYANQLGARQGAGAVYLNA
CCCCCEEEEECCHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
HHPDIMRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKNNEDMYLFSPYDVERVYGVA
CCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEECCCCCEEEECCCCHHHHHHHH
FSEISVTEKYREMVADSRIRKKKIKARDFFQVLAEIQFESGYPYIMFEDTVNRANPIAGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
ISMSNLCSEILQVSDVSTYNDDLSYKTMGKDISCNLGSLNIAAVMDSSDFGRTIETAIRA
CCHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHH
LTAVSDMSHISSVPSIEKGNDDSHAIGLGQMNLHGYLARERIFYGSEEGIDFTNMYFYTV
HHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHEEECCCCCCCEEEEEEEEE
TYHAIRASNRIAAERGTSFKGFENSKYASGEYFDKYTQTEWLPATRRVAELFETAGISIP
EEEHHHCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
TQEDWLALKEVVMASGLYNQNLQAVPPTGSISYINHSTSSIHPIVSKIEIRKEGKIGRVY
CCHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEE
YPAAFMSNDNLDYYQDAYEIGPEKIIDTYAAATQHVDQGLSLTLFFRDTATTRDINRAQI
ECEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHH
YAWKKGIKTIYYIRLRQMALAGTQVQGCVSCAL
HHHHHCCCEEEHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]