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The map label for this gene is nocT [C]

Identifier: 222102583

GI number: 222102583

Start: 190715

End: 191488

Strand: Direct

Name: nocT [C]

Synonym: Avi_7224

Alternate gene names: 222102583

Gene position: 190715-191488 (Clockwise)

Preceding gene: 222102582

Following gene: 222102584

Centisome position: 30.19

GC content: 50.65

Gene sequence:

>774_bases
ATGAAACTGCTTAAATCTCTTGCCACCGTAGCATTGCTGCAGGCTGCTTTACTCTTGCCCGCTCATGCAGGTGAAAATCT
CGCAACCATCAAATCGGCTGGCGTGTTCAAGATCGGTACCGAGGGCACTTATGCGCCATTCACCTATCACAATGAGAGTG
GCAAGCTGGTTGGTTTTGACGTGGAAATCGGAGAAGCCATTGCCGCCAAACTTGGTGTTAAAGCCGAGTTCGTGGAAGGA
AAATGGGATGGATTGATCGCCGGTCTCGATGCGAAGCGCTATGATACCGTGATCAATCAAGTGGGCATTACCGAGACGCG
CAAACAGAAATATGATTTTTCGGACCCCTATATCGCCTCAAAAGCCGTTCTGATCGTCCGCAGTGATGACAATAATATCA
AGGACTTTGCTGATCTGAAGGGCAAAAAATCGGCGCAATCTCTCAGCAGCAATTTTGGAAAGCTCGCTGAAAAGTCGGGT
GCGGAACTGGTTGGTACCGATGGGTTTGATCAGTCCATTCAACTCGTGCTGACGCGTCGTGCCGATGCCACGATCAATGA
CAGCCTGTCTTTCCTCGACTTCAAGAAGCACAAGCCAGATGCGCCGGTGAAGGTCGTCGCCCAGCAGAGCGATGCCGATT
ATTCCGGCGTGATCATTCGCAAGCAGGAGCCCGAATTGCTGACCGCCATCAACAAGGCGCTGGCTGACATCAAAGCCGAT
GGCACTTATCAGAAGATTTCTGAGAAGTATTTCGGTCAAGACGTCTCCAAATAG

Upstream 100 bases:

>100_bases
AAATATTTGCAATTGCTTATTTCTCGCGCAGTTTTATGCTCGTATTTTCGATGTGATTTCTGCGATTATCGGTTATCGCC
AATAAGGACGCATCACCGAT

Downstream 100 bases:

>100_bases
CGTTTTGTAGAAAGCTGGATATCGTTTAAACCGCCCTATATTCACGTGAAGAGCGCCCGGCGGGATTGTCTGCCGGGCGC
ATCTCTTTTGAGGAAGATGT

Product: ABC transporter substrate binding protein (nopaline)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKLLKSLATVALLQAALLLPAHAGENLATIKSAGVFKIGTEGTYAPFTYHNESGKLVGFDVEIGEAIAAKLGVKAEFVEG
KWDGLIAGLDAKRYDTVINQVGITETRKQKYDFSDPYIASKAVLIVRSDDNNIKDFADLKGKKSAQSLSSNFGKLAEKSG
AELVGTDGFDQSIQLVLTRRADATINDSLSFLDFKKHKPDAPVKVVAQQSDADYSGVIIRKQEPELLTAINKALADIKAD
GTYQKISEKYFGQDVSK

Sequences:

>Translated_257_residues
MKLLKSLATVALLQAALLLPAHAGENLATIKSAGVFKIGTEGTYAPFTYHNESGKLVGFDVEIGEAIAAKLGVKAEFVEG
KWDGLIAGLDAKRYDTVINQVGITETRKQKYDFSDPYIASKAVLIVRSDDNNIKDFADLKGKKSAQSLSSNFGKLAEKSG
AELVGTDGFDQSIQLVLTRRADATINDSLSFLDFKKHKPDAPVKVVAQQSDADYSGVIIRKQEPELLTAINKALADIKAD
GTYQKISEKYFGQDVSK
>Mature_257_residues
MKLLKSLATVALLQAALLLPAHAGENLATIKSAGVFKIGTEGTYAPFTYHNESGKLVGFDVEIGEAIAAKLGVKAEFVEG
KWDGLIAGLDAKRYDTVINQVGITETRKQKYDFSDPYIASKAVLIVRSDDNNIKDFADLKGKKSAQSLSSNFGKLAEKSG
AELVGTDGFDQSIQLVLTRRADATINDSLSFLDFKKHKPDAPVKVVAQQSDADYSGVIIRKQEPELLTAINKALADIKAD
GTYQKISEKYFGQDVSK

Specific function: Probably part of a binding-protein-dependent transport system for an amino acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=258, Percent_Identity=39.5348837209302, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1787085, Length=255, Percent_Identity=31.3725490196078, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=33.7606837606838, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI1788649, Length=227, Percent_Identity=31.7180616740088, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787031, Length=256, Percent_Identity=29.6875, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787088, Length=248, Percent_Identity=26.6129032258064, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27791; Mature: 27791

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLKSLATVALLQAALLLPAHAGENLATIKSAGVFKIGTEGTYAPFTYHNESGKLVGFD
CHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCCCCEEEECCCCCEEEEE
VEIGEAIAAKLGVKAEFVEGKWDGLIAGLDAKRYDTVINQVGITETRKQKYDFSDPYIAS
CHHHHHHHHHHCCEEEEECCCCCCEEECCCHHHHHHHHHHCCCCHHHHHCCCCCCCCCCC
KAVLIVRSDDNNIKDFADLKGKKSAQSLSSNFGKLAEKSGAELVGTDGFDQSIQLVLTRR
EEEEEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEEC
ADATINDSLSFLDFKKHKPDAPVKVVAQQSDADYSGVIIRKQEPELLTAINKALADIKAD
CCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHCCC
GTYQKISEKYFGQDVSK
CHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLLKSLATVALLQAALLLPAHAGENLATIKSAGVFKIGTEGTYAPFTYHNESGKLVGFD
CHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCCCCEEEECCCCCEEEEE
VEIGEAIAAKLGVKAEFVEGKWDGLIAGLDAKRYDTVINQVGITETRKQKYDFSDPYIAS
CHHHHHHHHHHCCEEEEECCCCCCEEECCCHHHHHHHHHHCCCCHHHHHCCCCCCCCCCC
KAVLIVRSDDNNIKDFADLKGKKSAQSLSSNFGKLAEKSGAELVGTDGFDQSIQLVLTRR
EEEEEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEEC
ADATINDSLSFLDFKKHKPDAPVKVVAQQSDADYSGVIIRKQEPELLTAINKALADIKAD
CCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHCCC
GTYQKISEKYFGQDVSK
CHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]