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The map label for this gene is yafV [C]

Identifier: 222102504

GI number: 222102504

Start: 98742

End: 99611

Strand: Reverse

Name: yafV [C]

Synonym: Avi_7123

Alternate gene names: 222102504

Gene position: 99611-98742 (Counterclockwise)

Preceding gene: 222102505

Following gene: 222102503

Centisome position: 15.77

GC content: 56.44

Gene sequence:

>870_bases
ATGACCTTCCGCGCCGCCGCCGTGCAGCTTGGCCCCGCAAGTGCCACGATCAAAGCCACGCTTGCCCGCATTCTAACCTT
GCTGGATAAGGCGGCAGAGCAAAAAATCACGCTGGCGGTGTTGCCGGAACTGGCGCTCACGCCTTATTTTGCTGCCACGA
TTCATGACCGCGCTGCTCAATATGCCGACAAGGAGGAGAATGATGCAGCAATTTCGGCAATAACAACCAAGGCCCGCGAT
TGCGCAATGACGGTGATCCTGCCGTTTGCCGAGCAGAACGGCAATCGCATCTACAATTCCATGGCCTTCATCAATAGCCG
AGGCGAGCATGTTGGCACCTTTCGCAAAATGCATATTCCAGGCTTCGTGGAGCAGAAGGAAAGTGGCGAAGTCACCATTC
TGGAAAAGCGCTACTTCGTACCTGGAGACAAGGGCTTTGCTGTGCATGACTGTGAACCCTGGAAGCTGGGTGGATTGATT
TGCTATGATCGGCGATTTCCCGAAGCCTATCGCTCGCTGTTTTTGCAGGGCGTAGATGTGATCTGTATTGGTTACAACAC
GCCCGTCATGCCCGCCAGCACGCTGTCCGCAAGCCGCCATGCCTCGGAACTTGCCATGTGTGGCGGGGCCTATTCAAACG
GCACCTATGTGGTCGCGGCGGGTAAAGCGGGCATGGAAGGCGGTTTACGCTACATCGGCGGCAGCGTGGTGATAGGCCCG
GACGGACGTCTTCTCAGCAAGGCCAAAACCATGGGAGACGAATTGGTGGTGGCTGACATCGATCTGCAAAGACAGCAGCT
CATTCGGGATAGATGGGACTTCCATGCCAACCGCCGACCGGATAGCTACCTGATGAGCGCAGCGGTCTGA

Upstream 100 bases:

>100_bases
CACGGAAACGCGGAAATGCTCTTTCTTTCGTTTATGTCTCTCCAGAGGAAAACTGGTTCCCGCTTCTTCTGGAAATGCTT
TTGCTGGAAAGAATGCCATC

Downstream 100 bases:

>100_bases
CGGCGCCAATGGCTTTCACGACACTCCGTTTGATGCTGGCAAACCGTTTTCCAGCCTTGCAGATCCGCAATTTCCGCATC
TTCTATCTCGGTCTGCTCTT

Product: D-N-carbamoylase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MTFRAAAVQLGPASATIKATLARILTLLDKAAEQKITLAVLPELALTPYFAATIHDRAAQYADKEENDAAISAITTKARD
CAMTVILPFAEQNGNRIYNSMAFINSRGEHVGTFRKMHIPGFVEQKESGEVTILEKRYFVPGDKGFAVHDCEPWKLGGLI
CYDRRFPEAYRSLFLQGVDVICIGYNTPVMPASTLSASRHASELAMCGGAYSNGTYVVAAGKAGMEGGLRYIGGSVVIGP
DGRLLSKAKTMGDELVVADIDLQRQQLIRDRWDFHANRRPDSYLMSAAV

Sequences:

>Translated_289_residues
MTFRAAAVQLGPASATIKATLARILTLLDKAAEQKITLAVLPELALTPYFAATIHDRAAQYADKEENDAAISAITTKARD
CAMTVILPFAEQNGNRIYNSMAFINSRGEHVGTFRKMHIPGFVEQKESGEVTILEKRYFVPGDKGFAVHDCEPWKLGGLI
CYDRRFPEAYRSLFLQGVDVICIGYNTPVMPASTLSASRHASELAMCGGAYSNGTYVVAAGKAGMEGGLRYIGGSVVIGP
DGRLLSKAKTMGDELVVADIDLQRQQLIRDRWDFHANRRPDSYLMSAAV
>Mature_288_residues
TFRAAAVQLGPASATIKATLARILTLLDKAAEQKITLAVLPELALTPYFAATIHDRAAQYADKEENDAAISAITTKARDC
AMTVILPFAEQNGNRIYNSMAFINSRGEHVGTFRKMHIPGFVEQKESGEVTILEKRYFVPGDKGFAVHDCEPWKLGGLIC
YDRRFPEAYRSLFLQGVDVICIGYNTPVMPASTLSASRHASELAMCGGAYSNGTYVVAAGKAGMEGGLRYIGGSVVIGPD
GRLLSKAKTMGDELVVADIDLQRQQLIRDRWDFHANRRPDSYLMSAAV

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=289, Percent_Identity=28.3737024221453, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI5031947, Length=269, Percent_Identity=27.5092936802974, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI297632348, Length=269, Percent_Identity=27.5092936802974, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI297632350, Length=269, Percent_Identity=27.5092936802974, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI7706509, Length=294, Percent_Identity=22.4489795918367, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322335, Length=315, Percent_Identity=25.7142857142857, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323383, Length=287, Percent_Identity=25.4355400696864, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21358471, Length=311, Percent_Identity=24.1157556270096, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 31499; Mature: 31367

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFRAAAVQLGPASATIKATLARILTLLDKAAEQKITLAVLPELALTPYFAATIHDRAAQ
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHCCHHHHHHHHHHHH
YADKEENDAAISAITTKARDCAMTVILPFAEQNGNRIYNSMAFINSRGEHVGTFRKMHIP
HCCCCCCCCEEHHHHHCCCCEEEEEEEEECCCCCCEEEHHHHHHCCCCCHHCCEEEECCC
GFVEQKESGEVTILEKRYFVPGDKGFAVHDCEPWKLGGLICYDRRFPEAYRSLFLQGVDV
CCCCCCCCCCEEEEEEEEECCCCCCEEEECCCCCEECCEEEECCCCHHHHHHHHHCCCCE
ICIGYNTPVMPASTLSASRHASELAMCGGAYSNGTYVVAAGKAGMEGGLRYIGGSVVIGP
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEECCEEEECC
DGRLLSKAKTMGDELVVADIDLQRQQLIRDRWDFHANRRPDSYLMSAAV
CCCHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHCCCC
>Mature Secondary Structure 
TFRAAAVQLGPASATIKATLARILTLLDKAAEQKITLAVLPELALTPYFAATIHDRAAQ
CEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHCCHHHHHHHHHHHH
YADKEENDAAISAITTKARDCAMTVILPFAEQNGNRIYNSMAFINSRGEHVGTFRKMHIP
HCCCCCCCCEEHHHHHCCCCEEEEEEEEECCCCCCEEEHHHHHHCCCCCHHCCEEEECCC
GFVEQKESGEVTILEKRYFVPGDKGFAVHDCEPWKLGGLICYDRRFPEAYRSLFLQGVDV
CCCCCCCCCCEEEEEEEEECCCCCCEEEECCCCCEECCEEEECCCCHHHHHHHHHCCCCE
ICIGYNTPVMPASTLSASRHASELAMCGGAYSNGTYVVAAGKAGMEGGLRYIGGSVVIGP
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEECCEEEECC
DGRLLSKAKTMGDELVVADIDLQRQQLIRDRWDFHANRRPDSYLMSAAV
CCCHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]