Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 222102417

GI number: 222102417

Start: 5807

End: 7513

Strand: Direct

Name: pleD [H]

Synonym: Avi_7005

Alternate gene names: 222102417

Gene position: 5807-7513 (Clockwise)

Preceding gene: 222102415

Following gene: 222102421

Centisome position: 0.92

GC content: 49.09

Gene sequence:

>1707_bases
ATGATATTGAACCACCAGTTTGGCAATCGTAAAAATAGTTTTGATTTGGACGCTGCAGCCGCGCTGGATGAAGCTTGGGA
CCTCGCTTGCAAGGGGCGTTCCGTTCGCGCATTTTCTTTAGCAAATTCAGTTTTGGCTTCGGCAGAACAGCGCGGTGATC
AAGGTCTTATAGCGGGGAGTTCTCAGCAATGTGCCTGGTATTGCTTTCAACTTGGTGAGACAGAGACGGGGCTAGTATAC
GTGGAGAAAGCAATTTCTCTGTGGAAGTCCCTTGAAGAGCCAATGCTTGAGGCACAAGCTCTTAGTATCGGTGCTTGGCT
GCATCTGGAACTCGGTAATCTAGAGCAGGCTGTCGAGTTTGCCGCAGAAGCACTCAACATTGCCGACCAAAGCCGTAATC
TCCGATCTCGGTCTTTGGCTATAAATGTGATTGGCGTGATATTCTGGATGACGCGTCAACCATCTCTTGCTGTTGACTAT
TGCAGTCGTGCCGTGGAGCTAGCCCGAGAGGCATACGATATTACATTCGAATGTTGGTGGTTGATTAATCTCGGTGGAGC
GCATGCAGAGGATGCTTATCTCGCTCAGGAAGCCGATAATCAAAAATTCGAAACGTCGATAAGCAGCGCAATAGATATTA
CGAAACAAGCGCTCAGCCTCGCTCAATCACTTAAAGACGCCTGGGCCGAGAGGTTGTGTATCGCAAATTTAGCAGAGTAT
TTTAATGCGGTTGAGGCTTTTTCCGCCGCCGAAATGTTATTGGAGCGGTACCGTGACATTCCGGGAGACGATTATGTCCG
CGGTGAGGGGCATTATCTGGTTACACTGGGAAGCACGCTCGTTTCGTTAAAGCGCTACGAAGAGGCATTGGTACCACTTG
AGCAGGCCCACTTGCTTGCCGGGGAAAATTTCAATCTTGAGACCTTAATGCATGCCTGTCTGTCTCTTTCAAAGGCTCAT
GAATATCTTGGAGCATTCGAGTCTGCGCTGGATTTTCATAAAAAGTATCACAAGCTCCATCAGCAGTTCACTGCGGAGCG
GATCCAGCGAAATGCCCGTATGGCGGAGATCCGTTATGAAACGAAAAAGCTCAAAGCAATGCTTGATACAGAGGCAGAGC
GCTCTGCAGAAATTGCCCGGTCATTAGAGGCCCTGCAACAGCAAACCAGTGTCCTGACCGAAGCGGCGAATACGGACCCG
TTGACAGGACTTTCCAATCGGCGGCATTTGGAGACGATAATATCTGACATTAATACTCAAAGCGCGAGTTATGCGCTGGC
GATCCTTGATGTCGATCACTTCAAGAAAATCAATGATACGTTTTCCCATATTATTGGTGATCGTGTGCTTGTGCAAATCG
GGACGCTGGTCAAAGTTGTTGTTCGTGAAACAGATATCGCAGTTCGCTTCGGTGGCGAAGAGTTCGTCATACTTATGACG
GACCTAACACTCGCCCACGCGGAAAGGGTTTCCGAAAGACTGCGAATGACAATTACCAACTTTGACTGGTCCGAAATTGC
CGATGGGCTGAATGTGTCCGTCAGTATCGGAGTGGCAATGTCAGACGATGCAGTCGACGCCGAAGCGGTTTTGCAACTGG
CGGATCGGCGCCTTTATTGGGCAAAGCAGACCGGTAGAAATAGAGTTGTTTGTTTTCTGCCGTCTGGAAATCGGTTTGAA
CAAACGACCAGCGCGATTACGGATTGA

Upstream 100 bases:

>100_bases
AGAAGGGGCCTTGGGAAAAACAGTGGCTTTAGGATTCCCTTAAATCGATCTCTGCTAAATTTGCTGGATATTCTGTCTGA
TCGTTGCGCAAGATGAGCTG

Downstream 100 bases:

>100_bases
GGAAGCGATCCGATTCTGCGGACTGCCTCTTAGCCTTCGCGACGTTGCTTTTCTCTTGCCATGAATCTCCGCTTCCGCCG
GAGTGAAAATGCGTACGGAA

Product: GGDEF family protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MILNHQFGNRKNSFDLDAAAALDEAWDLACKGRSVRAFSLANSVLASAEQRGDQGLIAGSSQQCAWYCFQLGETETGLVY
VEKAISLWKSLEEPMLEAQALSIGAWLHLELGNLEQAVEFAAEALNIADQSRNLRSRSLAINVIGVIFWMTRQPSLAVDY
CSRAVELAREAYDITFECWWLINLGGAHAEDAYLAQEADNQKFETSISSAIDITKQALSLAQSLKDAWAERLCIANLAEY
FNAVEAFSAAEMLLERYRDIPGDDYVRGEGHYLVTLGSTLVSLKRYEEALVPLEQAHLLAGENFNLETLMHACLSLSKAH
EYLGAFESALDFHKKYHKLHQQFTAERIQRNARMAEIRYETKKLKAMLDTEAERSAEIARSLEALQQQTSVLTEAANTDP
LTGLSNRRHLETIISDINTQSASYALAILDVDHFKKINDTFSHIIGDRVLVQIGTLVKVVVRETDIAVRFGGEEFVILMT
DLTLAHAERVSERLRMTITNFDWSEIADGLNVSVSIGVAMSDDAVDAEAVLQLADRRLYWAKQTGRNRVVCFLPSGNRFE
QTTSAITD

Sequences:

>Translated_568_residues
MILNHQFGNRKNSFDLDAAAALDEAWDLACKGRSVRAFSLANSVLASAEQRGDQGLIAGSSQQCAWYCFQLGETETGLVY
VEKAISLWKSLEEPMLEAQALSIGAWLHLELGNLEQAVEFAAEALNIADQSRNLRSRSLAINVIGVIFWMTRQPSLAVDY
CSRAVELAREAYDITFECWWLINLGGAHAEDAYLAQEADNQKFETSISSAIDITKQALSLAQSLKDAWAERLCIANLAEY
FNAVEAFSAAEMLLERYRDIPGDDYVRGEGHYLVTLGSTLVSLKRYEEALVPLEQAHLLAGENFNLETLMHACLSLSKAH
EYLGAFESALDFHKKYHKLHQQFTAERIQRNARMAEIRYETKKLKAMLDTEAERSAEIARSLEALQQQTSVLTEAANTDP
LTGLSNRRHLETIISDINTQSASYALAILDVDHFKKINDTFSHIIGDRVLVQIGTLVKVVVRETDIAVRFGGEEFVILMT
DLTLAHAERVSERLRMTITNFDWSEIADGLNVSVSIGVAMSDDAVDAEAVLQLADRRLYWAKQTGRNRVVCFLPSGNRFE
QTTSAITD
>Mature_568_residues
MILNHQFGNRKNSFDLDAAAALDEAWDLACKGRSVRAFSLANSVLASAEQRGDQGLIAGSSQQCAWYCFQLGETETGLVY
VEKAISLWKSLEEPMLEAQALSIGAWLHLELGNLEQAVEFAAEALNIADQSRNLRSRSLAINVIGVIFWMTRQPSLAVDY
CSRAVELAREAYDITFECWWLINLGGAHAEDAYLAQEADNQKFETSISSAIDITKQALSLAQSLKDAWAERLCIANLAEY
FNAVEAFSAAEMLLERYRDIPGDDYVRGEGHYLVTLGSTLVSLKRYEEALVPLEQAHLLAGENFNLETLMHACLSLSKAH
EYLGAFESALDFHKKYHKLHQQFTAERIQRNARMAEIRYETKKLKAMLDTEAERSAEIARSLEALQQQTSVLTEAANTDP
LTGLSNRRHLETIISDINTQSASYALAILDVDHFKKINDTFSHIIGDRVLVQIGTLVKVVVRETDIAVRFGGEEFVILMT
DLTLAHAERVSERLRMTITNFDWSEIADGLNVSVSIGVAMSDDAVDAEAVLQLADRRLYWAKQTGRNRVVCFLPSGNRFE
QTTSAITD

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=191, Percent_Identity=34.5549738219895, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI145693134, Length=157, Percent_Identity=39.4904458598726, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI87082007, Length=159, Percent_Identity=40.251572327044, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787816, Length=162, Percent_Identity=34.5679012345679, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081881, Length=163, Percent_Identity=34.9693251533742, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787541, Length=170, Percent_Identity=32.3529411764706, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786584, Length=155, Percent_Identity=32.258064516129, Blast_Score=86, Evalue=9e-18,
Organism=Escherichia coli, GI1788381, Length=168, Percent_Identity=29.7619047619048, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787056, Length=125, Percent_Identity=39.2, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788956, Length=172, Percent_Identity=33.1395348837209, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788085, Length=175, Percent_Identity=29.1428571428571, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI87081977, Length=167, Percent_Identity=29.940119760479, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1787802, Length=162, Percent_Identity=29.6296296296296, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081974, Length=156, Percent_Identity=30.7692307692308, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 63360; Mature: 63360

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILNHQFGNRKNSFDLDAAAALDEAWDLACKGRSVRAFSLANSVLASAEQRGDQGLIAGS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCCEEECC
SQQCAWYCFQLGETETGLVYVEKAISLWKSLEEPMLEAQALSIGAWLHLELGNLEQAVEF
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCHHHHHHH
AAEALNIADQSRNLRSRSLAINVIGVIFWMTRQPSLAVDYCSRAVELAREAYDITFECWW
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEE
LINLGGAHAEDAYLAQEADNQKFETSISSAIDITKQALSLAQSLKDAWAERLCIANLAEY
EEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FNAVEAFSAAEMLLERYRDIPGDDYVRGEGHYLVTLGSTLVSLKRYEEALVPLEQAHLLA
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEECHHHHHHHHHHHHHCCHHHHHHHC
GENFNLETLMHACLSLSKAHEYLGAFESALDFHKKYHKLHQQFTAERIQRNARMAEIRYE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
TKKLKAMLDTEAERSAEIARSLEALQQQTSVLTEAANTDPLTGLSNRRHLETIISDINTQ
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
SASYALAILDVDHFKKINDTFSHIIGDRVLVQIGTLVKVVVRETDIAVRFGGEEFVILMT
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEEE
DLTLAHAERVSERLRMTITNFDWSEIADGLNVSVSIGVAMSDDAVDAEAVLQLADRRLYW
CHHHHHHHHHHHHHHEEECCCCHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHEE
AKQTGRNRVVCFLPSGNRFEQTTSAITD
HHHCCCCEEEEEECCCCCHHHHHHHCCC
>Mature Secondary Structure
MILNHQFGNRKNSFDLDAAAALDEAWDLACKGRSVRAFSLANSVLASAEQRGDQGLIAGS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCCEEECC
SQQCAWYCFQLGETETGLVYVEKAISLWKSLEEPMLEAQALSIGAWLHLELGNLEQAVEF
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCHHHHHHH
AAEALNIADQSRNLRSRSLAINVIGVIFWMTRQPSLAVDYCSRAVELAREAYDITFECWW
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEE
LINLGGAHAEDAYLAQEADNQKFETSISSAIDITKQALSLAQSLKDAWAERLCIANLAEY
EEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FNAVEAFSAAEMLLERYRDIPGDDYVRGEGHYLVTLGSTLVSLKRYEEALVPLEQAHLLA
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEECHHHHHHHHHHHHHCCHHHHHHHC
GENFNLETLMHACLSLSKAHEYLGAFESALDFHKKYHKLHQQFTAERIQRNARMAEIRYE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
TKKLKAMLDTEAERSAEIARSLEALQQQTSVLTEAANTDPLTGLSNRRHLETIISDINTQ
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
SASYALAILDVDHFKKINDTFSHIIGDRVLVQIGTLVKVVVRETDIAVRFGGEEFVILMT
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEEE
DLTLAHAERVSERLRMTITNFDWSEIADGLNVSVSIGVAMSDDAVDAEAVLQLADRRLYW
CHHHHHHHHHHHHHHEEECCCCHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHEE
AKQTGRNRVVCFLPSGNRFEQTTSAITD
HHHCCCCEEEEEECCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA