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The map label for this gene is topA [H]

Identifier: 222097278

GI number: 222097278

Start: 3580307

End: 3582088

Strand: Reverse

Name: topA [H]

Synonym: BCQ_3618

Alternate gene names: 222097278

Gene position: 3582088-3580307 (Counterclockwise)

Preceding gene: 222097279

Following gene: 222097277

Centisome position: 68.7

GC content: 38.78

Gene sequence:

>1782_bases
GTGGACGTTGAATCAGATTGCCGAGTTGTGTTTAATGAGATTACAAAAGATGCAATAAAGGAATCCTTCAAACATCCTCG
CGCGATTAACATGAATTTAGTAGATGCACAACAAGCAAGACGTATACTAGATCGTCTTGTTGGTTACAATATTAGTCCTT
TATTATGGAAGAAAGTAAAAAAAGGATTAAGTGCAGGACGCGTACAATCTGTAGCAGTTCGTTTAATTATCGAACGTGAA
AGAGAAATTCAAAGTTTCGAGCCTGAAGAATTCTGGACAATTAAGACAGAATTTGTGAAAGGGAAAGACACATTTGAAGC
AAGCTTTTACGGTGTAGATGGTGAAAAAGTTCAATTAACGAATGAAACACAAGTGAATGAAATAATTGAACGGCTGAAAG
ATAATGCGTTCTCAGTTGAAAATGTAATGAGAAAAGAGCGAAAACGTAATCCGGCATTACCATTTACAACATCTTCCTTG
CAACAAGAGGCGGCGCGTAAGTTAAACATGCGAGCAAAGAAAACGATGATGCTTGCGCAGCAATTGTATGAAGGAATTGA
TCTTGGAAAACAAGGAACTGTAGGTCTTATTACGTATATGAGAACTGATTCAACACGTATCTCAGAAACAGCTCAAACAG
AGGCACGTACGTACATTACTGAGGCGTTTGGTACGGAATACATAGGAACAGAAAAGAAGAAAGAAACGAAGAAGTCGAAC
GCACAAGATGCGCATGAGGCAATTCGTCCTACTTCGGTAATGAGAAAGCCAGAGGAACTAAAGAGTTTCTTAAGTCGTGA
TCAACTTCGATTGTATAAATTGATTTGGGAGCGATTTGTTGCAAGTCAAATGGCGTCTGCTATAATGGATACTGTGACAG
CGAGACTCATTAATAATAATGTTCAGTTCCGTGCAAGTGGATCGGTTGTAAAGTTCCCAGGATTTATGAAAGTGTATGTA
GAGTCGAAAGATGATGGGGCTGAAGAGAAGGATAAGATGTTGCCACCTTTAGAAGTAGGGGAAACCGTATTTTCGAAGGA
TTTAGAACCGAAGCAACACTTTACACAACCTCCGCCGCGCTATACAGAGGCTCGTTTAGTAAGAACACTTGAAGAGCTTG
GAATTGGAAGACCGTCGACTTACGTACCGACACTTGAAACGATTCAAAAACGTGGATATGTAGGTTTGGATAATAAACGC
TTCGTTCCAACTGAACTAGGTGAAATAGTAATTGAACTTATTTTAGAGTTTTTCCCGGAAATTATTAACATTGAATTTAC
TGCTAACATGGAGCAAAGCCTCGATGAAGTAGAAGAAGGAAATGCGAATTGGGTAAAAATTGTTGATGATTTCTACGTAG
GATTTGAACCGCGTTTAGAAAAAGCGGAAAAAGAAATGCGTGAAGTGGAAATTAAAGATGAACCAGCTGGGGAAGATTGT
GAATTATGTAATCACCCAATGGTCTTTAAAATGGGTAAATACGGGAAATTTATGGCTTGCTCGAATTTCCCAGATTGTCG
TAATACAAAACCGATTGTGAAAGAAATCGGTGTTACTTGTCCGAAATGTGATAAAGGTCAAATTATTGAGCGTCGTAGTA
ATAAAAAGAAACGTCTTTTCTATGGGTGCGGTACGTATCCAGAGTGTGACTTTGTATCTTGGGATAAGCCGATTGGCCGT
AAATGTCCGAAGTGTGAAGGTATGCTAGTAGAGAAGAAGTTGAAAAAAGGCGTACAAGTACAATGTATTTCGTGTGATTA
TGAAGAAGAACAACAAATGTGA

Upstream 100 bases:

>100_bases
CTTAAAATCAGCAGCAAAAAAAGCAAAGAAAGTCTATCTCGCGGCCGATCCAGACCGCGAAGGGGAAGCTATTGCTTGGC
ATTTAGCCAATACGTTAAAT

Downstream 100 bases:

>100_bases
GCGTAACTGCTCACATTTTTTTCGGGAAGAAAAGGAAAGGTGATAAATATATGACAACACAAGTAGTAAACGTCATTGGC
GCAGGTCTTGCAGGAAGTGA

Product: DNA topoisomerase i

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MDVESDCRVVFNEITKDAIKESFKHPRAINMNLVDAQQARRILDRLVGYNISPLLWKKVKKGLSAGRVQSVAVRLIIERE
REIQSFEPEEFWTIKTEFVKGKDTFEASFYGVDGEKVQLTNETQVNEIIERLKDNAFSVENVMRKERKRNPALPFTTSSL
QQEAARKLNMRAKKTMMLAQQLYEGIDLGKQGTVGLITYMRTDSTRISETAQTEARTYITEAFGTEYIGTEKKKETKKSN
AQDAHEAIRPTSVMRKPEELKSFLSRDQLRLYKLIWERFVASQMASAIMDTVTARLINNNVQFRASGSVVKFPGFMKVYV
ESKDDGAEEKDKMLPPLEVGETVFSKDLEPKQHFTQPPPRYTEARLVRTLEELGIGRPSTYVPTLETIQKRGYVGLDNKR
FVPTELGEIVIELILEFFPEIINIEFTANMEQSLDEVEEGNANWVKIVDDFYVGFEPRLEKAEKEMREVEIKDEPAGEDC
ELCNHPMVFKMGKYGKFMACSNFPDCRNTKPIVKEIGVTCPKCDKGQIIERRSNKKKRLFYGCGTYPECDFVSWDKPIGR
KCPKCEGMLVEKKLKKGVQVQCISCDYEEEQQM

Sequences:

>Translated_593_residues
MDVESDCRVVFNEITKDAIKESFKHPRAINMNLVDAQQARRILDRLVGYNISPLLWKKVKKGLSAGRVQSVAVRLIIERE
REIQSFEPEEFWTIKTEFVKGKDTFEASFYGVDGEKVQLTNETQVNEIIERLKDNAFSVENVMRKERKRNPALPFTTSSL
QQEAARKLNMRAKKTMMLAQQLYEGIDLGKQGTVGLITYMRTDSTRISETAQTEARTYITEAFGTEYIGTEKKKETKKSN
AQDAHEAIRPTSVMRKPEELKSFLSRDQLRLYKLIWERFVASQMASAIMDTVTARLINNNVQFRASGSVVKFPGFMKVYV
ESKDDGAEEKDKMLPPLEVGETVFSKDLEPKQHFTQPPPRYTEARLVRTLEELGIGRPSTYVPTLETIQKRGYVGLDNKR
FVPTELGEIVIELILEFFPEIINIEFTANMEQSLDEVEEGNANWVKIVDDFYVGFEPRLEKAEKEMREVEIKDEPAGEDC
ELCNHPMVFKMGKYGKFMACSNFPDCRNTKPIVKEIGVTCPKCDKGQIIERRSNKKKRLFYGCGTYPECDFVSWDKPIGR
KCPKCEGMLVEKKLKKGVQVQCISCDYEEEQQM
>Mature_593_residues
MDVESDCRVVFNEITKDAIKESFKHPRAINMNLVDAQQARRILDRLVGYNISPLLWKKVKKGLSAGRVQSVAVRLIIERE
REIQSFEPEEFWTIKTEFVKGKDTFEASFYGVDGEKVQLTNETQVNEIIERLKDNAFSVENVMRKERKRNPALPFTTSSL
QQEAARKLNMRAKKTMMLAQQLYEGIDLGKQGTVGLITYMRTDSTRISETAQTEARTYITEAFGTEYIGTEKKKETKKSN
AQDAHEAIRPTSVMRKPEELKSFLSRDQLRLYKLIWERFVASQMASAIMDTVTARLINNNVQFRASGSVVKFPGFMKVYV
ESKDDGAEEKDKMLPPLEVGETVFSKDLEPKQHFTQPPPRYTEARLVRTLEELGIGRPSTYVPTLETIQKRGYVGLDNKR
FVPTELGEIVIELILEFFPEIINIEFTANMEQSLDEVEEGNANWVKIVDDFYVGFEPRLEKAEKEMREVEIKDEPAGEDC
ELCNHPMVFKMGKYGKFMACSNFPDCRNTKPIVKEIGVTCPKCDKGQIIERRSNKKKRLFYGCGTYPECDFVSWDKPIGR
KCPKCEGMLVEKKLKKGVQVQCISCDYEEEQQM

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=541, Percent_Identity=25.6931608133087, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI4507635, Length=554, Percent_Identity=22.7436823104693, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI1787529, Length=630, Percent_Identity=38.8888888888889, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI48994931, Length=111, Percent_Identity=39.6396396396396, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1788061, Length=471, Percent_Identity=21.656050955414, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32563869, Length=453, Percent_Identity=24.5033112582781, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17555378, Length=465, Percent_Identity=23.6559139784946, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323263, Length=503, Percent_Identity=25.6461232604374, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24585251, Length=576, Percent_Identity=24.4791666666667, Blast_Score=122, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24640096, Length=534, Percent_Identity=24.5318352059925, Blast_Score=115, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 68183; Mature: 68183

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVESDCRVVFNEITKDAIKESFKHPRAINMNLVDAQQARRILDRLVGYNISPLLWKKVK
CCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCEECHHHHHHHHHHHHCCCCCHHHHHHHH
KGLSAGRVQSVAVRLIIEREREIQSFEPEEFWTIKTEFVKGKDTFEASFYGVDGEKVQLT
HCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCCCEEEEECCCCCEEEEC
NETQVNEIIERLKDNAFSVENVMRKERKRNPALPFTTSSLQQEAARKLNMRAKKTMMLAQ
CCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QLYEGIDLGKQGTVGLITYMRTDSTRISETAQTEARTYITEAFGTEYIGTEKKKETKKSN
HHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCC
AQDAHEAIRPTSVMRKPEELKSFLSRDQLRLYKLIWERFVASQMASAIMDTVTARLINNN
HHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VQFRASGSVVKFPGFMKVYVESKDDGAEEKDKMLPPLEVGETVFSKDLEPKQHFTQPPPR
EEEEECCCEEECCCEEEEEEECCCCCCCHHHHCCCCHHHHHHHHHCCCCCHHHCCCCCCC
YTEARLVRTLEELGIGRPSTYVPTLETIQKRGYVGLDNKRFVPTELGEIVIELILEFFPE
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IINIEFTANMEQSLDEVEEGNANWVKIVDDFYVGFEPRLEKAEKEMREVEIKDEPAGEDC
HHEEEEECCHHHHHHHHHCCCCCEEEEEEHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCH
ELCNHPMVFKMGKYGKFMACSNFPDCRNTKPIVKEIGVTCPKCDKGQIIERRSNKKKRLF
HHHCCCCEEEECCCCCEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHCCCCCEEE
YGCGTYPECDFVSWDKPIGRKCPKCEGMLVEKKLKKGVQVQCISCDYEEEQQM
EECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCHHHHCC
>Mature Secondary Structure
MDVESDCRVVFNEITKDAIKESFKHPRAINMNLVDAQQARRILDRLVGYNISPLLWKKVK
CCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCEECHHHHHHHHHHHHCCCCCHHHHHHHH
KGLSAGRVQSVAVRLIIEREREIQSFEPEEFWTIKTEFVKGKDTFEASFYGVDGEKVQLT
HCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCCCEEEEECCCCCEEEEC
NETQVNEIIERLKDNAFSVENVMRKERKRNPALPFTTSSLQQEAARKLNMRAKKTMMLAQ
CCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QLYEGIDLGKQGTVGLITYMRTDSTRISETAQTEARTYITEAFGTEYIGTEKKKETKKSN
HHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCC
AQDAHEAIRPTSVMRKPEELKSFLSRDQLRLYKLIWERFVASQMASAIMDTVTARLINNN
HHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VQFRASGSVVKFPGFMKVYVESKDDGAEEKDKMLPPLEVGETVFSKDLEPKQHFTQPPPR
EEEEECCCEEECCCEEEEEEECCCCCCCHHHHCCCCHHHHHHHHHCCCCCHHHCCCCCCC
YTEARLVRTLEELGIGRPSTYVPTLETIQKRGYVGLDNKRFVPTELGEIVIELILEFFPE
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IINIEFTANMEQSLDEVEEGNANWVKIVDDFYVGFEPRLEKAEKEMREVEIKDEPAGEDC
HHEEEEECCHHHHHHHHHCCCCCEEEEEEHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCH
ELCNHPMVFKMGKYGKFMACSNFPDCRNTKPIVKEIGVTCPKCDKGQIIERRSNKKKRLF
HHHCCCCEEEECCCCCEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHCCCCCEEE
YGCGTYPECDFVSWDKPIGRKCPKCEGMLVEKKLKKGVQVQCISCDYEEEQQM
EECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]