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The map label for this gene is nemA [H]

Identifier: 222097107

GI number: 222097107

Start: 3393306

End: 3394433

Strand: Reverse

Name: nemA [H]

Synonym: BCQ_3447

Alternate gene names: 222097107

Gene position: 3394433-3393306 (Counterclockwise)

Preceding gene: 222097108

Following gene: 222097106

Centisome position: 65.1

GC content: 39.27

Gene sequence:

>1128_bases
ATGGCAAGTTCTAATAATAAAGCGGCAAGTATTTATGAAGGAACAAATGTAAACGCTTGGGGATCTTTTGAAAATATTGA
AGAAACGAAGTTGTTTGATCAGATTCAAGTCGGAGCTTGGTCTCTTCGTAATCGCATAGCAATGGCACCGATGACGAGAT
GCTTTGCAGACAATGAAACTGGAGTAGTTGGTGCTGATGTAGTGGAGTACTATAGAAAGCGTGCTGCTGACGGAGTTGGA
TTAATTATTACAGAAGGAATTATCATTAGTCCGAGAGCGAAAGGCAATCCAGGAGTACCAGGGATTTATACGCAAGAACA
AATCGACTCTTGGAAACCTGTTACAGAGGCAGTACATAAAGAAGGCGGGACGATTATTGCTCAAATATGGCATGTTGGAC
GTATGAGTCATCACGAAATAATTGGTGGGCAAATGCCGCAAGCGCCTTCTGCCATCGCTGCAGAAGGGAATGTTCCACGT
TTTCGCAAACCATTTGATACACCAGAAGCAATGACGTTACAAGAAATACAAGAAGTTATTAGTCAATACGCACAAGCTGC
AAAAAATGCGATTGAAGCTGGGTTTGATGGGGTAGAAATTCACGGTGCACACGGATATTTAATTGATCAATTTACTTATG
AGTTTGCAAATAAGCGTACTGATAAATATGGCGGTGACTTAAAGCAAAGATTAACGTTTATGAAAGAAGTAACTGAGGCT
GTAATAGAAGCAGTTGGAGCTGATAAAACACTTCTTCGTTTCTCTGCTTTTAAAGGGGATAATCCTACTTATATGTGGGA
AAACCCTGAGCTGGCAATTGAAACATTTGTAAATATGTTTAAAGAAGTTGGATTAACGATGATTCATCCTTCAACGATGA
ATTATACGCAAGTGATTGCTGACGGAAAGAATTTTCATCAATTAGTAAGAAAATATTGGGAGGGTACGATTGTTGGGGTT
GGTAATTTGAATCCGAAAGAAGCTGAAGAAGCGTTGCAAGAAGGAACAATTGACGTAGCTGCATTCGGAAGACCGTTAAT
TGCTAATCCAGATTTTGTTCATCGAATTAAACATGCTGAAAGTTTAGTTGAATATGACGCGAAAGAGCATCTTGCTACAT
TAATTTGA

Upstream 100 bases:

>100_bases
GGATATTCGAATGTTTTATAGAAAAGTCGTATAAATTGACGTTACTATTTAAAAGTAAGTATGATGCGATTATAGCAAGA
ATTTTTAAGGGGGAAGAAAA

Downstream 100 bases:

>100_bases
ATAAAGATAAAAGGAGAACACATGGAGTGTTCTCCTTTTATTATGGAATTTCATAGGGGGAAGCGAGATGAGTGAAACGC
TACTCGTTATTATTAGCATA

Product: NADH-dependent flavin oxidoreductase, oye family

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MASSNNKAASIYEGTNVNAWGSFENIEETKLFDQIQVGAWSLRNRIAMAPMTRCFADNETGVVGADVVEYYRKRAADGVG
LIITEGIIISPRAKGNPGVPGIYTQEQIDSWKPVTEAVHKEGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNVPR
FRKPFDTPEAMTLQEIQEVISQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANKRTDKYGGDLKQRLTFMKEVTEA
VIEAVGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMNYTQVIADGKNFHQLVRKYWEGTIVGV
GNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKHAESLVEYDAKEHLATLI

Sequences:

>Translated_375_residues
MASSNNKAASIYEGTNVNAWGSFENIEETKLFDQIQVGAWSLRNRIAMAPMTRCFADNETGVVGADVVEYYRKRAADGVG
LIITEGIIISPRAKGNPGVPGIYTQEQIDSWKPVTEAVHKEGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNVPR
FRKPFDTPEAMTLQEIQEVISQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANKRTDKYGGDLKQRLTFMKEVTEA
VIEAVGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMNYTQVIADGKNFHQLVRKYWEGTIVGV
GNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKHAESLVEYDAKEHLATLI
>Mature_374_residues
ASSNNKAASIYEGTNVNAWGSFENIEETKLFDQIQVGAWSLRNRIAMAPMTRCFADNETGVVGADVVEYYRKRAADGVGL
IITEGIIISPRAKGNPGVPGIYTQEQIDSWKPVTEAVHKEGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNVPRF
RKPFDTPEAMTLQEIQEVISQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANKRTDKYGGDLKQRLTFMKEVTEAV
IEAVGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMNYTQVIADGKNFHQLVRKYWEGTIVGVG
NLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKHAESLVEYDAKEHLATLI

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=341, Percent_Identity=39.0029325513196, Blast_Score=224, Evalue=5e-60,
Organism=Escherichia coli, GI1789463, Length=345, Percent_Identity=30.7246376811594, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17565138, Length=354, Percent_Identity=28.8135593220339, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI72001454, Length=358, Percent_Identity=24.3016759776536, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17559802, Length=326, Percent_Identity=26.3803680981595, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17564188, Length=310, Percent_Identity=24.8387096774194, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559804, Length=323, Percent_Identity=24.4582043343653, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=342, Percent_Identity=25.4385964912281, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17566914, Length=215, Percent_Identity=30.2325581395349, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321973, Length=361, Percent_Identity=31.5789473684211, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6325086, Length=362, Percent_Identity=30.6629834254144, Blast_Score=166, Evalue=5e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 41532; Mature: 41401

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSNNKAASIYEGTNVNAWGSFENIEETKLFDQIQVGAWSLRNRIAMAPMTRCFADNET
CCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GVVGADVVEYYRKRAADGVGLIITEGIIISPRAKGNPGVPGIYTQEQIDSWKPVTEAVHK
CCCHHHHHHHHHHHCCCCCEEEEECCEEECCCCCCCCCCCCCCCHHHHHCCCHHHHHHHH
EGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNVPRFRKPFDTPEAMTLQEIQEVI
CCCEEEHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
SQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANKRTDKYGGDLKQRLTFMKEVTEA
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHH
VIEAVGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMNYTQVIA
HHHHHCCCHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCCEEEECCCCCCHHHHHH
DGKNFHQLVRKYWEGTIVGVGNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKHAE
CCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHH
SLVEYDAKEHLATLI
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
ASSNNKAASIYEGTNVNAWGSFENIEETKLFDQIQVGAWSLRNRIAMAPMTRCFADNET
CCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GVVGADVVEYYRKRAADGVGLIITEGIIISPRAKGNPGVPGIYTQEQIDSWKPVTEAVHK
CCCHHHHHHHHHHHCCCCCEEEEECCEEECCCCCCCCCCCCCCCHHHHHCCCHHHHHHHH
EGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNVPRFRKPFDTPEAMTLQEIQEVI
CCCEEEHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
SQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANKRTDKYGGDLKQRLTFMKEVTEA
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHH
VIEAVGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMNYTQVIA
HHHHHCCCHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCCEEEECCCCCCHHHHHH
DGKNFHQLVRKYWEGTIVGVGNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKHAE
CCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHH
SLVEYDAKEHLATLI
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]