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The map label for this gene is alkK [H]

Identifier: 222097087

GI number: 222097087

Start: 3373963

End: 3375576

Strand: Reverse

Name: alkK [H]

Synonym: BCQ_3427

Alternate gene names: 222097087

Gene position: 3375576-3373963 (Counterclockwise)

Preceding gene: 222097088

Following gene: 222097086

Centisome position: 64.74

GC content: 38.72

Gene sequence:

>1614_bases
ATGATGATGAATGTACCGCTAACAATTAGTTCTATGATGGAAAGAGCAGAAAAATTGTTTTCAAAGAAAGAAATTGTTTC
ACGGACACATGATACAGTTACGACGTTAACGTATAAGCAGTTAGGTGAAAGGACGAGAAGGCTTTCCAGTGCGTTAAAAA
AATTAGGAATTAAAGAAGGCGAGCGTATAGGAACGTTAGCGTGGAATCATCATCGACATGTGGAAGCGTATTTTGCTATT
CCTGGTATTGCTTCCGTTTTACATACAATTAATATTCGTTTATCTCCTCAACATATTTCATACATTATTCAGCACGCAGA
AGATCGAATTCTACTTATTGATGAAGATCTCGTACCACTTGTTGAAAAAATTCAATCTGAATTATCAACTGTACAAGCTT
ACATTATTATGACTGATAAAGATGAAATTCCAAATACTACGCTAGAACCTGTATATCATTATGAAAAGCTATTAGAAGAA
GGCGATCCAACTTTCCAATTCGTTAAAGATATTGATGAAAATACACCTGCTGGTATGTGTTATACGTCAGCGACTACAGG
AAATCCAAAAGGTGTTGTATATACGCATCGTAGTACTGTATTGCATTGTATGGCACTCGGTTTAGCAGATACAGCTGCAT
TATCGGAAAGTGATGCGGCAATGGCGATTGTACCGATGTTCCATGTGAATGCTTGGGGACTTCCTTTCGCTGCTACTTGG
TTTGGATCAAAACAAGTTCTTCCAGGACCGATGTTTACGCCGAAAATTTTATTAGAAATGATCCAATCTGAAAAAGTGAC
GTTAGCTGCCGGTGTGCCGACAATTTGGCTTGGTGTATTACAAGAGTTAGAAAATAATAGTTATGATTTATCTAGTATGA
CGAGAATACTATGTGGTGGTGCAGCCGCACCGAAAAGTGTTATTAAAGCATTTGAACAGAAACACAATGTTCCTTTCATC
CATGCATATGGTATGACCGAAAGAAGCCCACTCGTAACACTTGCACGTTTAAAAAGTTATGAAACAGAATTATCGTATGA
AGAGCAATTAGAAATTAGATCAAAGCAAGGGTATCTTGTCCCTGGTGTAGAAATGAAAGTAGTCGGTACAAATGGTGAAG
TGAAGTGGGATAGTACGGAGATGGGAGAACTATGTTTACGAGCGCCATGGATCGCTGAAAGCTATTATAACGATGATCGT
ACTGTTGAAGGCTTTCGCGATGGATGGTTATATACTGGTGATGTTGTCACAGTGGACGAGGAAGGCTGCGTAAAAATTGT
TGACCGTACGAAAGATGTGATTAAAAGCGGAGGCGAGTGGATTTCTTCAGTAGACCTTGAAAATGCTTTAATGGCACATG
ATGCTATATTTGAAGCGGCTGTCGTTGCTGTTCCTCATCCGCAGTGGCAAGAGCGACCAGTTGCCTGTGTCGTTCAAAAG
AAAAATAGTACAGTAACAAAAGAAGAAATATATGAGTTTTTAAAACCACAGTTTGCAAAGTGGTGGTTACCAGACGATAT
TGTGTTTATGGAAGAAATACCGAAAACATCTGTTGGGAAGTTTTTAAAACAGGCGCTTCGGAAAGAACTCGAGCATTTGC
ATAAAGAGAAATAA

Upstream 100 bases:

>100_bases
AAAGGAATAATTCAGAAAATACAGAAAAATGTCGTGATTATTTGTAGAAGTCTGTTATAATAAACAAAATGAAAACGCTT
TAAAGGGGGATGTAACCAAT

Downstream 100 bases:

>100_bases
TGGAATTTATATTTGTAAACCTAATTTAAAAATAAGTTGACAAATACTAGATAGAAAGATAATATAACTCCTATAATATG
TTACCTAAAAAAAAATTTGA

Product: medium-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MMMNVPLTISSMMERAEKLFSKKEIVSRTHDTVTTLTYKQLGERTRRLSSALKKLGIKEGERIGTLAWNHHRHVEAYFAI
PGIASVLHTINIRLSPQHISYIIQHAEDRILLIDEDLVPLVEKIQSELSTVQAYIIMTDKDEIPNTTLEPVYHYEKLLEE
GDPTFQFVKDIDENTPAGMCYTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDAAMAIVPMFHVNAWGLPFAATW
FGSKQVLPGPMFTPKILLEMIQSEKVTLAAGVPTIWLGVLQELENNSYDLSSMTRILCGGAAAPKSVIKAFEQKHNVPFI
HAYGMTERSPLVTLARLKSYETELSYEEQLEIRSKQGYLVPGVEMKVVGTNGEVKWDSTEMGELCLRAPWIAESYYNDDR
TVEGFRDGWLYTGDVVTVDEEGCVKIVDRTKDVIKSGGEWISSVDLENALMAHDAIFEAAVVAVPHPQWQERPVACVVQK
KNSTVTKEEIYEFLKPQFAKWWLPDDIVFMEEIPKTSVGKFLKQALRKELEHLHKEK

Sequences:

>Translated_537_residues
MMMNVPLTISSMMERAEKLFSKKEIVSRTHDTVTTLTYKQLGERTRRLSSALKKLGIKEGERIGTLAWNHHRHVEAYFAI
PGIASVLHTINIRLSPQHISYIIQHAEDRILLIDEDLVPLVEKIQSELSTVQAYIIMTDKDEIPNTTLEPVYHYEKLLEE
GDPTFQFVKDIDENTPAGMCYTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDAAMAIVPMFHVNAWGLPFAATW
FGSKQVLPGPMFTPKILLEMIQSEKVTLAAGVPTIWLGVLQELENNSYDLSSMTRILCGGAAAPKSVIKAFEQKHNVPFI
HAYGMTERSPLVTLARLKSYETELSYEEQLEIRSKQGYLVPGVEMKVVGTNGEVKWDSTEMGELCLRAPWIAESYYNDDR
TVEGFRDGWLYTGDVVTVDEEGCVKIVDRTKDVIKSGGEWISSVDLENALMAHDAIFEAAVVAVPHPQWQERPVACVVQK
KNSTVTKEEIYEFLKPQFAKWWLPDDIVFMEEIPKTSVGKFLKQALRKELEHLHKEK
>Mature_537_residues
MMMNVPLTISSMMERAEKLFSKKEIVSRTHDTVTTLTYKQLGERTRRLSSALKKLGIKEGERIGTLAWNHHRHVEAYFAI
PGIASVLHTINIRLSPQHISYIIQHAEDRILLIDEDLVPLVEKIQSELSTVQAYIIMTDKDEIPNTTLEPVYHYEKLLEE
GDPTFQFVKDIDENTPAGMCYTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDAAMAIVPMFHVNAWGLPFAATW
FGSKQVLPGPMFTPKILLEMIQSEKVTLAAGVPTIWLGVLQELENNSYDLSSMTRILCGGAAAPKSVIKAFEQKHNVPFI
HAYGMTERSPLVTLARLKSYETELSYEEQLEIRSKQGYLVPGVEMKVVGTNGEVKWDSTEMGELCLRAPWIAESYYNDDR
TVEGFRDGWLYTGDVVTVDEEGCVKIVDRTKDVIKSGGEWISSVDLENALMAHDAIFEAAVVAVPHPQWQERPVACVVQK
KNSTVTKEEIYEFLKPQFAKWWLPDDIVFMEEIPKTSVGKFLKQALRKELEHLHKEK

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=554, Percent_Identity=27.0758122743682, Blast_Score=182, Evalue=1e-45,
Organism=Homo sapiens, GI187761345, Length=549, Percent_Identity=26.5938069216758, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI187761343, Length=549, Percent_Identity=26.5938069216758, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI38505220, Length=509, Percent_Identity=25.147347740668, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI42544132, Length=512, Percent_Identity=23.6328125, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI157311624, Length=514, Percent_Identity=23.5408560311284, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI157311622, Length=514, Percent_Identity=23.5408560311284, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI28416953, Length=539, Percent_Identity=23.5621521335807, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI58082049, Length=520, Percent_Identity=23.0769230769231, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI115511026, Length=514, Percent_Identity=21.7898832684825, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI122937307, Length=516, Percent_Identity=22.4806201550388, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI83745141, Length=300, Percent_Identity=25.3333333333333, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788107, Length=520, Percent_Identity=25.5769230769231, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI145693145, Length=363, Percent_Identity=31.9559228650138, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI221142682, Length=513, Percent_Identity=23.3918128654971, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI1786810, Length=524, Percent_Identity=24.0458015267176, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789201, Length=507, Percent_Identity=23.0769230769231, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1788595, Length=499, Percent_Identity=22.8456913827655, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1790505, Length=539, Percent_Identity=22.2634508348794, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17531443, Length=524, Percent_Identity=25.1908396946565, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17559526, Length=360, Percent_Identity=31.9444444444444, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17558820, Length=515, Percent_Identity=25.631067961165, Blast_Score=154, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI71994690, Length=533, Percent_Identity=25.891181988743, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71994694, Length=533, Percent_Identity=25.891181988743, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI71994703, Length=533, Percent_Identity=25.891181988743, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17557194, Length=510, Percent_Identity=24.3137254901961, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17560308, Length=536, Percent_Identity=25.3731343283582, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17538037, Length=515, Percent_Identity=24.2718446601942, Blast_Score=139, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI32563687, Length=505, Percent_Identity=24.950495049505, Blast_Score=131, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17560140, Length=506, Percent_Identity=22.7272727272727, Blast_Score=118, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71996755, Length=513, Percent_Identity=24.7563352826511, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=372, Percent_Identity=22.8494623655914, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319699, Length=364, Percent_Identity=27.4725274725275, Blast_Score=130, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6319264, Length=401, Percent_Identity=24.4389027431421, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21355181, Length=502, Percent_Identity=27.2908366533865, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24581924, Length=551, Percent_Identity=25.9528130671506, Blast_Score=152, Evalue=7e-37,
Organism=Drosophila melanogaster, GI18859661, Length=359, Percent_Identity=27.2980501392758, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI21356441, Length=532, Percent_Identity=23.4962406015038, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24648255, Length=516, Percent_Identity=22.8682170542636, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24648253, Length=510, Percent_Identity=22.3529411764706, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24653035, Length=511, Percent_Identity=22.3091976516634, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24648260, Length=496, Percent_Identity=21.7741935483871, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI19922652, Length=521, Percent_Identity=21.8809980806142, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24656500, Length=530, Percent_Identity=21.5094339622642, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17933690, Length=498, Percent_Identity=23.6947791164659, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI281365686, Length=522, Percent_Identity=19.7318007662835, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI161076582, Length=523, Percent_Identity=21.9885277246654, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24648257, Length=543, Percent_Identity=22.4677716390424, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20130357, Length=521, Percent_Identity=20.7293666026871, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21358303, Length=500, Percent_Identity=20.2, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21356947, Length=521, Percent_Identity=21.1132437619962, Blast_Score=80, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 60501; Mature: 60501

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMNVPLTISSMMERAEKLFSKKEIVSRTHDTVTTLTYKQLGERTRRLSSALKKLGIKEG
CEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ERIGTLAWNHHRHVEAYFAIPGIASVLHTINIRLSPQHISYIIQHAEDRILLIDEDLVPL
CEEEEEEECCCCCEEEEEECCHHHHHHHHHEEEECHHHHHHHHHHCCCCEEEECCCHHHH
VEKIQSELSTVQAYIIMTDKDEIPNTTLEPVYHYEKLLEEGDPTFQFVKDIDENTPAGMC
HHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEE
YTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDAAMAIVPMFHVNAWGLPFAATW
EECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEEEEE
FGSKQVLPGPMFTPKILLEMIQSEKVTLAAGVPTIWLGVLQELENNSYDLSSMTRILCGG
CCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHCC
AAAPKSVIKAFEQKHNVPFIHAYGMTERSPLVTLARLKSYETELSYEEQLEIRSKQGYLV
CCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEE
PGVEMKVVGTNGEVKWDSTEMGELCLRAPWIAESYYNDDRTVEGFRDGWLYTGDVVTVDE
CCCEEEEEECCCEEEECCCHHHHHHHCCCHHHHHHCCCCCCCCHHCCCCEEECCEEEECC
EGCVKIVDRTKDVIKSGGEWISSVDLENALMAHDAIFEAAVVAVPHPQWQERPVACVVQK
CCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEEC
KNSTVTKEEIYEFLKPQFAKWWLPDDIVFMEEIPKTSVGKFLKQALRKELEHLHKEK
CCCCCCHHHHHHHHCCHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMMNVPLTISSMMERAEKLFSKKEIVSRTHDTVTTLTYKQLGERTRRLSSALKKLGIKEG
CEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ERIGTLAWNHHRHVEAYFAIPGIASVLHTINIRLSPQHISYIIQHAEDRILLIDEDLVPL
CEEEEEEECCCCCEEEEEECCHHHHHHHHHEEEECHHHHHHHHHHCCCCEEEECCCHHHH
VEKIQSELSTVQAYIIMTDKDEIPNTTLEPVYHYEKLLEEGDPTFQFVKDIDENTPAGMC
HHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEE
YTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDAAMAIVPMFHVNAWGLPFAATW
EECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEEEEE
FGSKQVLPGPMFTPKILLEMIQSEKVTLAAGVPTIWLGVLQELENNSYDLSSMTRILCGG
CCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHCC
AAAPKSVIKAFEQKHNVPFIHAYGMTERSPLVTLARLKSYETELSYEEQLEIRSKQGYLV
CCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEE
PGVEMKVVGTNGEVKWDSTEMGELCLRAPWIAESYYNDDRTVEGFRDGWLYTGDVVTVDE
CCCEEEEEECCCEEEECCCHHHHHHHCCCHHHHHHCCCCCCCCHHCCCCEEECCEEEECC
EGCVKIVDRTKDVIKSGGEWISSVDLENALMAHDAIFEAAVVAVPHPQWQERPVACVVQK
CCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEEC
KNSTVTKEEIYEFLKPQFAKWWLPDDIVFMEEIPKTSVGKFLKQALRKELEHLHKEK
CCCCCCHHHHHHHHCCHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]