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The map label for this gene is dctA [H]

Identifier: 222097041

GI number: 222097041

Start: 3330509

End: 3331771

Strand: Reverse

Name: dctA [H]

Synonym: BCQ_3381

Alternate gene names: 222097041

Gene position: 3331771-3330509 (Counterclockwise)

Preceding gene: 222097042

Following gene: 222097040

Centisome position: 63.9

GC content: 35.79

Gene sequence:

>1263_bases
ATGAGAATCGTAAAGAATTTAACATTTCAAGTTATTGTGGCCATTATTTGTGGTATTGCAGTAGGAGCTATTTGGCCGAG
TGTTGGACAAGAGATGAAGCCAATTGGTGAAACGTTCATCAATATGATTAAAATGGTTATTGCACCGATTATCTTTTTAA
CAATTGTGCTTGGTATTGCAAGCATGGGAAGTATGAAAAAGGTAGGTCGTGTTGGTGGTAAGGCATTATTATATTTTGAA
ATTGTTACAACTGCGGCTTTAATTATTGGTATCATCATAGCGAATGTTGTACGTCCCGGAGATGGATTAGATCCCTCAAA
GCTAAAGGGCGGAGATGTTTCGCAATATGTACAAAGCGGGCAAGAAATGAAGTGGGTGGATTTCTTTTTACATATTGTAC
CGTCTAACATGTTTGAAGCATTTGCAAAAGGTGATATTTTACAAGTGCTGTTCTTCTCCATTTTATTCGGTGCTGGGTTA
ACGATGTTAGGGAAAAATGGACAACCGGTAATCGATTTTTTTGAAAGGTTATCGAAAGTATTTTTTAACATTTTATCAAT
TGTAATGAAATTAGCACCGATTGGAGCATTCGGCGGGATGGCGTTTACAATTGGCAAATATGGTTTAAGTACACTTATTC
CACTTGGTAAATTAATGATTTGTGTATATGCGACAATGGCATTATTTGTTTTTATTGTTTTAAATTTTATTTGTAAACTG
TACAAATTTAGTTTGTGGAAATATTTAGCTCACATTAAAGAAGAATTACTCATTGTTCTTGGGACATCATCATCAGAATC
AGTATTACCGCGAATGATGACAAAGATGGAAGACTTCGGATGTTCGAAGCCGGTAGTAGGTTTAGTCATTCCGACGGGAT
ACTCTTTTAATTTAGATGGAACGACAATTTATTTATCGATGGCTACTATATTTTTAGCACAAGTCTTTCACGTCGATCTT
TCACTAGGTCAACAATTAACGATAATAGCTATTTTGTTAGTTACATCTAAAGGAGCAGCCGCAGTAACAGGTGGAGGCTT
TATTGTACTAGCGTCTACTTTATCTGCGATGAATGTTATTCCATTAGAAGGGTTAGCGCTATTACTTGGTGTAGATCGTT
TCATGTCAGAAGCACGTGCTATCGTCAATTTAATTGGTAACGGTATTGCGACTGTAGTTGTTGCAAAAAGTGAAAATGAA
TTTGATGAAGAGAAGTATACGAGAGTAATAGAGGAAATGAAAAAAGAGAAAATGGTCGGATAA

Upstream 100 bases:

>100_bases
TAGTTTAAGTTACATAACAGGGGGGCTATTACATAGAATATATCATTAGCAATTATTGAAAAAGTATGGGAGAAATGAAA
GAAGCAGGGGAGTATCAAAA

Downstream 100 bases:

>100_bases
AAAGTCAGCTTTGCTGACTTTTTTATTTTTGTAGAAAAGAGGTTTGTTACGATATACATTATTTATTAACAATAATTTAC
AGGTGTATTATAGTGGAAGG

Product: c4-dicarboxylate transporter dcta

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: C4-dicarboxylate permease [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MRIVKNLTFQVIVAIICGIAVGAIWPSVGQEMKPIGETFINMIKMVIAPIIFLTIVLGIASMGSMKKVGRVGGKALLYFE
IVTTAALIIGIIIANVVRPGDGLDPSKLKGGDVSQYVQSGQEMKWVDFFLHIVPSNMFEAFAKGDILQVLFFSILFGAGL
TMLGKNGQPVIDFFERLSKVFFNILSIVMKLAPIGAFGGMAFTIGKYGLSTLIPLGKLMICVYATMALFVFIVLNFICKL
YKFSLWKYLAHIKEELLIVLGTSSSESVLPRMMTKMEDFGCSKPVVGLVIPTGYSFNLDGTTIYLSMATIFLAQVFHVDL
SLGQQLTIIAILLVTSKGAAAVTGGGFIVLASTLSAMNVIPLEGLALLLGVDRFMSEARAIVNLIGNGIATVVVAKSENE
FDEEKYTRVIEEMKKEKMVG

Sequences:

>Translated_420_residues
MRIVKNLTFQVIVAIICGIAVGAIWPSVGQEMKPIGETFINMIKMVIAPIIFLTIVLGIASMGSMKKVGRVGGKALLYFE
IVTTAALIIGIIIANVVRPGDGLDPSKLKGGDVSQYVQSGQEMKWVDFFLHIVPSNMFEAFAKGDILQVLFFSILFGAGL
TMLGKNGQPVIDFFERLSKVFFNILSIVMKLAPIGAFGGMAFTIGKYGLSTLIPLGKLMICVYATMALFVFIVLNFICKL
YKFSLWKYLAHIKEELLIVLGTSSSESVLPRMMTKMEDFGCSKPVVGLVIPTGYSFNLDGTTIYLSMATIFLAQVFHVDL
SLGQQLTIIAILLVTSKGAAAVTGGGFIVLASTLSAMNVIPLEGLALLLGVDRFMSEARAIVNLIGNGIATVVVAKSENE
FDEEKYTRVIEEMKKEKMVG
>Mature_420_residues
MRIVKNLTFQVIVAIICGIAVGAIWPSVGQEMKPIGETFINMIKMVIAPIIFLTIVLGIASMGSMKKVGRVGGKALLYFE
IVTTAALIIGIIIANVVRPGDGLDPSKLKGGDVSQYVQSGQEMKWVDFFLHIVPSNMFEAFAKGDILQVLFFSILFGAGL
TMLGKNGQPVIDFFERLSKVFFNILSIVMKLAPIGAFGGMAFTIGKYGLSTLIPLGKLMICVYATMALFVFIVLNFICKL
YKFSLWKYLAHIKEELLIVLGTSSSESVLPRMMTKMEDFGCSKPVVGLVIPTGYSFNLDGTTIYLSMATIFLAQVFHVDL
SLGQQLTIIAILLVTSKGAAAVTGGGFIVLASTLSAMNVIPLEGLALLLGVDRFMSEARAIVNLIGNGIATVVVAKSENE
FDEEKYTRVIEEMKKEKMVG

Specific function: Responsible for the transport of succinate and fumarate, but not malate, across the membrane (Probable) [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=449, Percent_Identity=24.9443207126949, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI21314632, Length=441, Percent_Identity=26.3038548752834, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI40254478, Length=478, Percent_Identity=24.0585774058577, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI5032093, Length=421, Percent_Identity=26.1282660332542, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI223468564, Length=283, Percent_Identity=28.6219081272085, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI66773030, Length=427, Percent_Identity=25.2927400468384, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI223468566, Length=283, Percent_Identity=28.6219081272085, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI194239697, Length=432, Percent_Identity=24.537037037037, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI4827012, Length=277, Percent_Identity=27.0758122743682, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI262359914, Length=376, Percent_Identity=24.2021276595745, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI301601644, Length=183, Percent_Identity=28.4153005464481, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1789947, Length=414, Percent_Identity=55.7971014492754, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI1790514, Length=414, Percent_Identity=40.5797101449275, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI1789473, Length=324, Percent_Identity=23.1481481481481, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17537407, Length=465, Percent_Identity=26.6666666666667, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI71983099, Length=420, Percent_Identity=25.2380952380952, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71983106, Length=420, Percent_Identity=25.2380952380952, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI193206505, Length=406, Percent_Identity=26.8472906403941, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI71996953, Length=421, Percent_Identity=25.41567695962, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI193206654, Length=437, Percent_Identity=24.7139588100687, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17541374, Length=436, Percent_Identity=24.3119266055046, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24583025, Length=400, Percent_Identity=24.25, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17137668, Length=400, Percent_Identity=24.25, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24583023, Length=400, Percent_Identity=24.25, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI281360481, Length=419, Percent_Identity=23.8663484486874, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281360483, Length=419, Percent_Identity=23.8663484486874, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17137666, Length=419, Percent_Identity=23.8663484486874, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 45519; Mature: 45519

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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>Mature Secondary Structure
MRIVKNLTFQVIVAIICGIAVGAIWPSVGQEMKPIGETFINMIKMVIAPIIFLTIVLGIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMGSMKKVGRVGGKALLYFEIVTTAALIIGIIIANVVRPGDGLDPSKLKGGDVSQYVQSG
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCC
QEMKWVDFFLHIVPSNMFEAFAKGDILQVLFFSILFGAGLTMLGKNGQPVIDFFERLSKV
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHH
FFNILSIVMKLAPIGAFGGMAFTIGKYGLSTLIPLGKLMICVYATMALFVFIVLNFICKL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YKFSLWKYLAHIKEELLIVLGTSSSESVLPRMMTKMEDFGCSKPVVGLVIPTGYSFNLDG
HHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCC
TTIYLSMATIFLAQVFHVDLSLGQQLTIIAILLVTSKGAAAVTGGGFIVLASTLSAMNVI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHCCC
PLEGLALLLGVDRFMSEARAIVNLIGNGIATVVVAKSENEFDEEKYTRVIEEMKKEKMVG
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 10708364 [H]