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The map label for this gene is oppA [H]

Identifier: 222097040

GI number: 222097040

Start: 3328449

End: 3330146

Strand: Reverse

Name: oppA [H]

Synonym: BCQ_3380

Alternate gene names: 222097040

Gene position: 3330146-3328449 (Counterclockwise)

Preceding gene: 222097041

Following gene: 222097039

Centisome position: 63.87

GC content: 36.28

Gene sequence:

>1698_bases
ATGAAAAAACTAACAGCAGTTGTAGCACCAGTTTTAGCAATGAGTATGGCATTAACAGCATGTTCTACATCAGGTGGAGA
TAAGAAGACAAGCACAAATTCTAGCTCAGGCGGAGATAGCAAATCAGAAGAAAAATTAGCAGCGAAACAAGTACTAAATC
GTACTGAAACAAATGAGATTCCAACAATGGATACTTCTAAAAATACAGATACATTAGGTTCTCAAATTTTAGGGAACACG
ATGGAAGGTTTATATCGATTAGATAAAGATAACAAACCAATCCCAGCTGCGGCAGAATCAAGCACGAAGAGTGAGGATGG
TAAAAAATATACATTCAAATTACGTAAAGATGCAAAATGGTCAAATGGTGATCCTGTAACAGCGAAAGATTTCGTATATG
CATGGCAACGTTTACTAGATCCAAAAACAGCTGCTGAGTATGCATTTATCGCATTCCCAATTAAAAATGCAGAAGCGGTT
AATAAAGGCGAAAAACCTGTAACGGAACTAGGAGTAAAGGCAGTAGACGATCTTACTTTAGAAGTTGAATTAGAACAAGC
AGTGCCATACTTCTTAAACTTAGTAGCATTCCCATCGTACTATCCGTTAAATGAGAAATTTGTAACAGAAAAAGGGGATA
AATTCGGTTTAGAGTCTGATACGACAGTATATAACGGACCGTTCGTTCTTACTGATTGGAAGCATGAGCAAGGCTGGAAA
CTAAAGAAAAACGATCAATATTGGGATAAAAAGACTGTAAAACTAGACGAAATTAACTATAGTGTAGTAAAAGAAGTAGC
GACAAGAGTAAACTTATATGATACAAATGCAATTGACTTCGCGCTATTATCGGGTGAATTCGTTGATAAGTATAGAAACA
ATAAAGAAGAGTTTGGCACATACTCACAAGTAAGTACTTACTTCTTACGTATGAACCAAAAACGTGGCGGGCAAGATACA
CCGTTAAAGAGCCAAAAATTACGTGAAGCAATTGCATTATCAATTGATAAAAAGAATTTATCAAATGTTATTTTAAATGA
TGGATCTAAGCCTGCGGACTTCTTAGTACCAAAAGGATTAGCGACTGGACCAGACGGTAAAGACTTCCAAGAAACATTTA
AAAATGGTATTAAACCAGATGCGAAAAAAGCAGCAGCTGCATGGGAAGAAGCGAAAAAAGAGCTTGGAAAAGACCAAGTT
ACAATTGAGTTATTAAACTATGACACTGGTAATGCGAAAAAAGTTGGGGAATATGTAAAAGATCAAATTGAGAAAAACTT
AAAAGGTGTAACTGTTAATATTAAACTTCAACCATTCAAACAAAAACTGAAATTAGAAACAGAGCAAGATTATGATTTAT
CATATGCTGGTTGGGGACCTGACTACGCTGACCCAATGACATTCTTAGATATGTTCCAGTCTAATCATTCTCATAACCAA
ATGAGCTTCGCTGATCCGAAATATGATGATATGATCAAAAAAGCTGGCGGAGAATTAATGGGTGATGCGAAGAAACGTTG
GGAAGAGCTAGGAAAAGCTGAGAAATTACTTCTTGAGCAAGATGTAGCACTTGTACCTTTATATCAACGTGGTGATGCAT
ACGTTCAAAAACCAAATGCAAAAGGCATCGTTCACCATAACATTAGCCCAGAGTATAGCTTCAAGTGGGCATATATGACT
GAAAAAGATGGGAAATAA

Upstream 100 bases:

>100_bases
AATCTTCTGATAAATATATAAAGAAAAACAGAATTATCAGAAAATTCTTAAATGAGAGCGTTTAGAATTAGGGAGGGTAC
AGAAAGTGAAAAAGAAAAAA

Downstream 100 bases:

>100_bases
TTTTAAAGAATAATACTATCTTGAAGTGGATGCATACAAGTTTCTTAGCTTATTTTGTAGTAAGAATCTCGTTGTCCACG
ATAGAAAGATAAAATGATAG

Product: ABC transporter substrate-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MKKLTAVVAPVLAMSMALTACSTSGGDKKTSTNSSSGGDSKSEEKLAAKQVLNRTETNEIPTMDTSKNTDTLGSQILGNT
MEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLRKDAKWSNGDPVTAKDFVYAWQRLLDPKTAAEYAFIAFPIKNAEAV
NKGEKPVTELGVKAVDDLTLEVELEQAVPYFLNLVAFPSYYPLNEKFVTEKGDKFGLESDTTVYNGPFVLTDWKHEQGWK
LKKNDQYWDKKTVKLDEINYSVVKEVATRVNLYDTNAIDFALLSGEFVDKYRNNKEEFGTYSQVSTYFLRMNQKRGGQDT
PLKSQKLREAIALSIDKKNLSNVILNDGSKPADFLVPKGLATGPDGKDFQETFKNGIKPDAKKAAAAWEEAKKELGKDQV
TIELLNYDTGNAKKVGEYVKDQIEKNLKGVTVNIKLQPFKQKLKLETEQDYDLSYAGWGPDYADPMTFLDMFQSNHSHNQ
MSFADPKYDDMIKKAGGELMGDAKKRWEELGKAEKLLLEQDVALVPLYQRGDAYVQKPNAKGIVHHNISPEYSFKWAYMT
EKDGK

Sequences:

>Translated_565_residues
MKKLTAVVAPVLAMSMALTACSTSGGDKKTSTNSSSGGDSKSEEKLAAKQVLNRTETNEIPTMDTSKNTDTLGSQILGNT
MEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLRKDAKWSNGDPVTAKDFVYAWQRLLDPKTAAEYAFIAFPIKNAEAV
NKGEKPVTELGVKAVDDLTLEVELEQAVPYFLNLVAFPSYYPLNEKFVTEKGDKFGLESDTTVYNGPFVLTDWKHEQGWK
LKKNDQYWDKKTVKLDEINYSVVKEVATRVNLYDTNAIDFALLSGEFVDKYRNNKEEFGTYSQVSTYFLRMNQKRGGQDT
PLKSQKLREAIALSIDKKNLSNVILNDGSKPADFLVPKGLATGPDGKDFQETFKNGIKPDAKKAAAAWEEAKKELGKDQV
TIELLNYDTGNAKKVGEYVKDQIEKNLKGVTVNIKLQPFKQKLKLETEQDYDLSYAGWGPDYADPMTFLDMFQSNHSHNQ
MSFADPKYDDMIKKAGGELMGDAKKRWEELGKAEKLLLEQDVALVPLYQRGDAYVQKPNAKGIVHHNISPEYSFKWAYMT
EKDGK
>Mature_565_residues
MKKLTAVVAPVLAMSMALTACSTSGGDKKTSTNSSSGGDSKSEEKLAAKQVLNRTETNEIPTMDTSKNTDTLGSQILGNT
MEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLRKDAKWSNGDPVTAKDFVYAWQRLLDPKTAAEYAFIAFPIKNAEAV
NKGEKPVTELGVKAVDDLTLEVELEQAVPYFLNLVAFPSYYPLNEKFVTEKGDKFGLESDTTVYNGPFVLTDWKHEQGWK
LKKNDQYWDKKTVKLDEINYSVVKEVATRVNLYDTNAIDFALLSGEFVDKYRNNKEEFGTYSQVSTYFLRMNQKRGGQDT
PLKSQKLREAIALSIDKKNLSNVILNDGSKPADFLVPKGLATGPDGKDFQETFKNGIKPDAKKAAAAWEEAKKELGKDQV
TIELLNYDTGNAKKVGEYVKDQIEKNLKGVTVNIKLQPFKQKLKLETEQDYDLSYAGWGPDYADPMTFLDMFQSNHSHNQ
MSFADPKYDDMIKKAGGELMGDAKKRWEELGKAEKLLLEQDVALVPLYQRGDAYVQKPNAKGIVHHNISPEYSFKWAYMT
EKDGK

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=550, Percent_Identity=31.6363636363636, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI87081878, Length=535, Percent_Identity=29.5327102803738, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1789397, Length=518, Percent_Identity=28.957528957529, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI1787762, Length=460, Percent_Identity=24.7826086956522, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1787052, Length=497, Percent_Identity=24.5472837022133, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789887, Length=515, Percent_Identity=24.6601941747573, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 63342; Mature: 63342

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTAVVAPVLAMSMALTACSTSGGDKKTSTNSSSGGDSKSEEKLAAKQVLNRTETNEI
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
PTMDTSKNTDTLGSQILGNTMEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLRKDAKW
CCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
SNGDPVTAKDFVYAWQRLLDPKTAAEYAFIAFPIKNAEAVNKGEKPVTELGVKAVDDLTL
CCCCCCCHHHHHHHHHHHCCCCHHHCEEEEEEECCCCHHHCCCCCCHHHHCCHHHCCEEE
EVELEQAVPYFLNLVAFPSYYPLNEKFVTEKGDKFGLESDTTVYNGPFVLTDWKHEQGWK
EEEHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCEEECCCEEEECCCCCCCCC
LKKNDQYWDKKTVKLDEINYSVVKEVATRVNLYDTNAIDFALLSGEFVDKYRNNKEEFGT
CCCCCCCCCCCCEEEECCCHHHHHHHHHHCCEEECCCEEEEECCCHHHHHHCCCHHHCCC
YSQVSTYFLRMNQKRGGQDTPLKSQKLREAIALSIDKKNLSNVILNDGSKPADFLVPKGL
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHCCCHHEECCCCCCCHHCCCCCC
ATGPDGKDFQETFKNGIKPDAKKAAAAWEEAKKELGKDQVTIELLNYDTGNAKKVGEYVK
CCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHH
DQIEKNLKGVTVNIKLQPFKQKLKLETEQDYDLSYAGWGPDYADPMTFLDMFQSNHSHNQ
HHHHCCCCEEEEEEEECCHHHHHCCCCCCCCCCEECCCCCCCCCHHHHHHHHHCCCCCCC
MSFADPKYDDMIKKAGGELMGDAKKRWEELGKAEKLLLEQDVALVPLYQRGDAYVQKPNA
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEECCCC
KGIVHHNISPEYSFKWAYMTEKDGK
CCEEECCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MKKLTAVVAPVLAMSMALTACSTSGGDKKTSTNSSSGGDSKSEEKLAAKQVLNRTETNEI
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
PTMDTSKNTDTLGSQILGNTMEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLRKDAKW
CCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
SNGDPVTAKDFVYAWQRLLDPKTAAEYAFIAFPIKNAEAVNKGEKPVTELGVKAVDDLTL
CCCCCCCHHHHHHHHHHHCCCCHHHCEEEEEEECCCCHHHCCCCCCHHHHCCHHHCCEEE
EVELEQAVPYFLNLVAFPSYYPLNEKFVTEKGDKFGLESDTTVYNGPFVLTDWKHEQGWK
EEEHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCEEECCCEEEECCCCCCCCC
LKKNDQYWDKKTVKLDEINYSVVKEVATRVNLYDTNAIDFALLSGEFVDKYRNNKEEFGT
CCCCCCCCCCCCEEEECCCHHHHHHHHHHCCEEECCCEEEEECCCHHHHHHCCCHHHCCC
YSQVSTYFLRMNQKRGGQDTPLKSQKLREAIALSIDKKNLSNVILNDGSKPADFLVPKGL
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHCCCHHEECCCCCCCHHCCCCCC
ATGPDGKDFQETFKNGIKPDAKKAAAAWEEAKKELGKDQVTIELLNYDTGNAKKVGEYVK
CCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHH
DQIEKNLKGVTVNIKLQPFKQKLKLETEQDYDLSYAGWGPDYADPMTFLDMFQSNHSHNQ
HHHHCCCCEEEEEEEECCHHHHHCCCCCCCCCCEECCCCCCCCCHHHHHHHHHCCCCCCC
MSFADPKYDDMIKKAGGELMGDAKKRWEELGKAEKLLLEQDVALVPLYQRGDAYVQKPNA
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEECCCC
KGIVHHNISPEYSFKWAYMTEKDGK
CCEEECCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]