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The map label for this gene is yfnB [H]

Identifier: 222096806

GI number: 222096806

Start: 3098449

End: 3099159

Strand: Reverse

Name: yfnB [H]

Synonym: BCQ_3146

Alternate gene names: 222096806

Gene position: 3099159-3098449 (Counterclockwise)

Preceding gene: 222096807

Following gene: 222096805

Centisome position: 59.44

GC content: 34.46

Gene sequence:

>711_bases
ATGAAATACAAAGTTATATTATTCGACGTAGACGATACATTATTAGATTTCCCTGAAACGGAAAGACACGCATTACATAA
TGCGTTTATACAGTTTGGTATGCCTACAGGGTATACTGATTATCTTGCAAGTTATAAAGAGATTAGTAATGGGTTATGGA
GAGATTTAGAAAATAAAATGATTACGTTAAGTGAATTAGCGGTAGATCGATTTAGACAATTATTTGCACTTCATAATATA
GAAGTAGATGCGCAGCAATTTAGTGATGTATATCTTGAAAACTTAGGGAAAGAAGTACATCTTATAGAAGGTGCAGTGCA
ATTATGTGAGAATCTACAAGATTGCAAGCTAGGCATCATTACGAATGGATATACGAAGGTGCAACAATCTAGAATCGGAA
ATTCGCCTTTATGTAATTTCTTTGATCATATTATTATTTCAGAAGAGGTTGGTCATCAAAAACCAGCACGTGAGATTTTT
GATTATGCATTTGAAAAGTTTGGGATTACGGATAAATCAAGTGTACTAATGGTTGGAGATTCGCTCACTTCTGATATGAA
AGGCGGAGAAGATTACGGCATTGATACGTGTTGGTATAATCCGAGTTTGAAAGAGAATAAGGCAAGTGTTAAGCCGACTT
ATGAAGTGAAGAGTCTGCTGCAAATTTTAGAATTTGTAGAAGTGGCGGAAGAAAAGGTAGTTTCATTTTAA

Upstream 100 bases:

>100_bases
TAGAAGATAAATAAAGAGGAGGGAGGCTGATTGGATTAGCCTCCTTTTCTTATTGTTGTTATAATTAGGCTATGAGAATA
TAGGAATTGAGGAGAACATC

Downstream 100 bases:

>100_bases
TCGTGTCGATATTTGTTGATAAGTCGATATTCTTTGTCGAATCGTCGATATATCGAAAAAATCGTTGATATATTTTAAGT
TACGGTAGATATATTGGCAA

Product: haloacid dehalogenase

Products: (R)-2-hydroxyacid; halide

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MKYKVILFDVDDTLLDFPETERHALHNAFIQFGMPTGYTDYLASYKEISNGLWRDLENKMITLSELAVDRFRQLFALHNI
EVDAQQFSDVYLENLGKEVHLIEGAVQLCENLQDCKLGIITNGYTKVQQSRIGNSPLCNFFDHIIISEEVGHQKPAREIF
DYAFEKFGITDKSSVLMVGDSLTSDMKGGEDYGIDTCWYNPSLKENKASVKPTYEVKSLLQILEFVEVAEEKVVSF

Sequences:

>Translated_236_residues
MKYKVILFDVDDTLLDFPETERHALHNAFIQFGMPTGYTDYLASYKEISNGLWRDLENKMITLSELAVDRFRQLFALHNI
EVDAQQFSDVYLENLGKEVHLIEGAVQLCENLQDCKLGIITNGYTKVQQSRIGNSPLCNFFDHIIISEEVGHQKPAREIF
DYAFEKFGITDKSSVLMVGDSLTSDMKGGEDYGIDTCWYNPSLKENKASVKPTYEVKSLLQILEFVEVAEEKVVSF
>Mature_236_residues
MKYKVILFDVDDTLLDFPETERHALHNAFIQFGMPTGYTDYLASYKEISNGLWRDLENKMITLSELAVDRFRQLFALHNI
EVDAQQFSDVYLENLGKEVHLIEGAVQLCENLQDCKLGIITNGYTKVQQSRIGNSPLCNFFDHIIISEEVGHQKPAREIF
DYAFEKFGITDKSSVLMVGDSLTSDMKGGEDYGIDTCWYNPSLKENKASVKPTYEVKSLLQILEFVEVAEEKVVSF

Specific function: Unknown

COG id: COG1011

COG function: function code R; Predicted hydrolase (HAD superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. YjjG family [H]

Homologues:

Organism=Escherichia coli, GI1790833, Length=223, Percent_Identity=35.4260089686099, Blast_Score=141, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR011951 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 3.8.1.2

Molecular weight: Translated: 26960; Mature: 26960

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYKVILFDVDDTLLDFPETERHALHNAFIQFGMPTGYTDYLASYKEISNGLWRDLENKM
CCEEEEEEECCHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHE
ITLSELAVDRFRQLFALHNIEVDAQQFSDVYLENLGKEVHLIEGAVQLCENLQDCKLGII
EHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCEEEEE
TNGYTKVQQSRIGNSPLCNFFDHIIISEEVGHQKPAREIFDYAFEKFGITDKSSVLMVGD
ECCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEECC
SLTSDMKGGEDYGIDTCWYNPSLKENKASVKPTYEVKSLLQILEFVEVAEEKVVSF
CHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKYKVILFDVDDTLLDFPETERHALHNAFIQFGMPTGYTDYLASYKEISNGLWRDLENKM
CCEEEEEEECCHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHE
ITLSELAVDRFRQLFALHNIEVDAQQFSDVYLENLGKEVHLIEGAVQLCENLQDCKLGII
EHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCEEEEE
TNGYTKVQQSRIGNSPLCNFFDHIIISEEVGHQKPAREIFDYAFEKFGITDKSSVLMVGD
ECCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEECC
SLTSDMKGGEDYGIDTCWYNPSLKENKASVKPTYEVKSLLQILEFVEVAEEKVVSF
CHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-haloacid; H2O

Specific reaction: (S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9141694; 9384377 [H]