Click here to switch to the map view.

The map label for this gene is amiB [H]

Identifier: 222096743

GI number: 222096743

Start: 3044489

End: 3046264

Strand: Reverse

Name: amiB [H]

Synonym: BCQ_3083

Alternate gene names: 222096743

Gene position: 3046264-3044489 (Counterclockwise)

Preceding gene: 222096745

Following gene: 222096742

Centisome position: 58.42

GC content: 38.23

Gene sequence:

>1776_bases
ATGAATATTAAAAAACGAGTAATCGCACTATCAACAAGTCTAGTAATTTTAGGGAGTGCACCTTTATCAATATCAGCAGA
AGAGATAAATAAATCTCATAATGACCAGCTTCGTATTGCACAAGGGGAGCGTGGAACGAACGTATTACGTTATTCTAATT
TCAATCTAGAAACTCCAGATCACATGCAAATTAATGAAGCAGAAGTATCAGAGAACGAGGAAATTCTTCTAGAAGAATAT
AAACATAAACATGATGAAACAGAAGAGTTACATAAAATGAAAGAAGCGAAAAAAGGTGAAGTAGCAGAAGATGAAGCCAT
TTCAAATATTGAAAAGGCATTTGAACATGTAGACGGTCGAAATGATGGTTCTACTCGCGAAATGAAGCCGGTTGAAAAGC
GAAGAGCTATTCAGTATAGCGCATCGATGACTACGAAGAAATATGGAACACTTGCAGGTGTCCCGTTTGTAGAGTGGATT
GTTCCAGCAGGAAACCCTGATATTCGCCCCGCTAATCCGATGAAAGCACGTTATATTACAATTCACGAAACTGCAAACAC
TGCCCGCGGTGCAAATGCTGAAAACCATGCGAAGTATTTATATAAACAAGCAACGGAAGGTACGTTCCGAACAGCTTCAT
GGCATTTTACTGTAGACGATAAACAAATTTATCAGCACTTACCAACAAATGAAAACGGTTGGCATGCAGGAGATGGGGAT
GGATCAGGAAATAGAGAATCTATCGGGATTGAAATTTCAGTTAACCAAGATGGAGATTACAATAAAGCGTTAGAGAATGC
AAAGAGACTTGCAGGTTATTTAATGAACAAAGAAGGTATTAGTGCGGACCACATTTATAGGCATCAACAATGGAGCGGGA
AAAACTGTCCTGATATTTTAATTTCTCGTGGGAATTGGACTGGCTTCGTACAAGGTATTCAATGGTATGCAAATGTTAAC
GCACAAATTGATTCGCCTTTACAAGAAAGAAATGAGCTATTTGATTCCAATGAATATAATCCAGCTGAACCTAATATGGA
GCTTGTAGTGAATGGTGATGGTATAAACGTACGTAGTGGTTCGGGACTTGAACATCATATCGTTCGTAAAGCTTCAAACG
GAGATCGTTACAAAGTGTTAGCAGTTAAAAATGGTTGGTATAAAGTAGGTAACGATGAGTGGATTTTCTATAACCCAAGT
TGGATTAAAATAAACTATAATATGCCACATAAAGAAGTACAAAAACCAAAAGATGATATTACAGGTGGATGGTTTGAAGG
GAACATTCGTAAGCTGAACAGCCTTGGAATTATGCACGGTGAAGGAAATGGTGTATTTGCGCCTTATCGTAATGTAACGA
GAGCTGAGTTTGCTGCATTAATTTCAAATGCTCTCAAGCTACCGGAAGGAGATAAGTCATTTGTAGACATTAACAAAGCG
CATCCATCACTTCATAGCGGTATTAAACGTAGTGCAAGTGCAGGAATTATTAACGGTCGTGGCGGCGGGATTTTTGATCC
TAACGCATCTATCACACGTGAAGAAGCATCGATCATGATTGACAACGCATTACAGTATAAAGGTGTTACAGGTGAATTAG
TTGCATTGTCATTTAGGGATAAACATTTGATCACATATAAACAGCCTGTTCAACGATTATATAGTTTAAAGGTAGTTACA
GGTGTTGGAAATAATGTGTTTAATCCGAAAGGCACGACAACTCGTGGTGAAGCAGCTGCCTTTTTAGTAAAGATGTTAGA
TGCAAGTCAAAAATAG

Upstream 100 bases:

>100_bases
ACCATATTTTTATACATAGTAGATTCATAGTCTTTCACGAATGAGAAAATAAAGGGAGGTTGAGATATTTACACGAACAG
TGTAGATATCGATTACAGCA

Downstream 100 bases:

>100_bases
AAAGAATGATAAAGGAGCTATCCAAAATGAGAATTTGGAACAGCTCCTTTTTATGTAAGCCCTTATTAAAGCCCCTTCCA
AGCCCCTTCCAAGCCCCTTC

Product: N-acetylmuramoyl-l-alanine amidase and s-layer protein fusion

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MNIKKRVIALSTSLVILGSAPLSISAEEINKSHNDQLRIAQGERGTNVLRYSNFNLETPDHMQINEAEVSENEEILLEEY
KHKHDETEELHKMKEAKKGEVAEDEAISNIEKAFEHVDGRNDGSTREMKPVEKRRAIQYSASMTTKKYGTLAGVPFVEWI
VPAGNPDIRPANPMKARYITIHETANTARGANAENHAKYLYKQATEGTFRTASWHFTVDDKQIYQHLPTNENGWHAGDGD
GSGNRESIGIEISVNQDGDYNKALENAKRLAGYLMNKEGISADHIYRHQQWSGKNCPDILISRGNWTGFVQGIQWYANVN
AQIDSPLQERNELFDSNEYNPAEPNMELVVNGDGINVRSGSGLEHHIVRKASNGDRYKVLAVKNGWYKVGNDEWIFYNPS
WIKINYNMPHKEVQKPKDDITGGWFEGNIRKLNSLGIMHGEGNGVFAPYRNVTRAEFAALISNALKLPEGDKSFVDINKA
HPSLHSGIKRSASAGIINGRGGGIFDPNASITREEASIMIDNALQYKGVTGELVALSFRDKHLITYKQPVQRLYSLKVVT
GVGNNVFNPKGTTTRGEAAAFLVKMLDASQK

Sequences:

>Translated_591_residues
MNIKKRVIALSTSLVILGSAPLSISAEEINKSHNDQLRIAQGERGTNVLRYSNFNLETPDHMQINEAEVSENEEILLEEY
KHKHDETEELHKMKEAKKGEVAEDEAISNIEKAFEHVDGRNDGSTREMKPVEKRRAIQYSASMTTKKYGTLAGVPFVEWI
VPAGNPDIRPANPMKARYITIHETANTARGANAENHAKYLYKQATEGTFRTASWHFTVDDKQIYQHLPTNENGWHAGDGD
GSGNRESIGIEISVNQDGDYNKALENAKRLAGYLMNKEGISADHIYRHQQWSGKNCPDILISRGNWTGFVQGIQWYANVN
AQIDSPLQERNELFDSNEYNPAEPNMELVVNGDGINVRSGSGLEHHIVRKASNGDRYKVLAVKNGWYKVGNDEWIFYNPS
WIKINYNMPHKEVQKPKDDITGGWFEGNIRKLNSLGIMHGEGNGVFAPYRNVTRAEFAALISNALKLPEGDKSFVDINKA
HPSLHSGIKRSASAGIINGRGGGIFDPNASITREEASIMIDNALQYKGVTGELVALSFRDKHLITYKQPVQRLYSLKVVT
GVGNNVFNPKGTTTRGEAAAFLVKMLDASQK
>Mature_591_residues
MNIKKRVIALSTSLVILGSAPLSISAEEINKSHNDQLRIAQGERGTNVLRYSNFNLETPDHMQINEAEVSENEEILLEEY
KHKHDETEELHKMKEAKKGEVAEDEAISNIEKAFEHVDGRNDGSTREMKPVEKRRAIQYSASMTTKKYGTLAGVPFVEWI
VPAGNPDIRPANPMKARYITIHETANTARGANAENHAKYLYKQATEGTFRTASWHFTVDDKQIYQHLPTNENGWHAGDGD
GSGNRESIGIEISVNQDGDYNKALENAKRLAGYLMNKEGISADHIYRHQQWSGKNCPDILISRGNWTGFVQGIQWYANVN
AQIDSPLQERNELFDSNEYNPAEPNMELVVNGDGINVRSGSGLEHHIVRKASNGDRYKVLAVKNGWYKVGNDEWIFYNPS
WIKINYNMPHKEVQKPKDDITGGWFEGNIRKLNSLGIMHGEGNGVFAPYRNVTRAEFAALISNALKLPEGDKSFVDINKA
HPSLHSGIKRSASAGIINGRGGGIFDPNASITREEASIMIDNALQYKGVTGELVALSFRDKHLITYKQPVQRLYSLKVVT
GVGNNVFNPKGTTTRGEAAAFLVKMLDASQK

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 65946; Mature: 65946

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKKRVIALSTSLVILGSAPLSISAEEINKSHNDQLRIAQGERGTNVLRYSNFNLETPD
CCCHHEEEEEEEEEEEEECCCCEEEHHHHCCCCCCCEEEECCCCCCEEEEEECCCCCCCC
HMQINEAEVSENEEILLEEYKHKHDETEELHKMKEAKKGEVAEDEAISNIEKAFEHVDGR
CCEECHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
NDGSTREMKPVEKRRAIQYSASMTTKKYGTLAGVPFVEWIVPAGNPDIRPANPMKARYIT
CCCCCCCCCHHHHHHHEEHHHHCCHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEE
IHETANTARGANAENHAKYLYKQATEGTFRTASWHFTVDDKQIYQHLPTNENGWHAGDGD
EEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEECHHHHHHHCCCCCCCCCCCCCC
GSGNRESIGIEISVNQDGDYNKALENAKRLAGYLMNKEGISADHIYRHQQWSGKNCPDIL
CCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCCCCCCHHHE
ISRGNWTGFVQGIQWYANVNAQIDSPLQERNELFDSNEYNPAEPNMELVVNGDGINVRSG
EECCCCCCHHEEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCEEECCC
SGLEHHIVRKASNGDRYKVLAVKNGWYKVGNDEWIFYNPSWIKINYNMPHKEVQKPKDDI
CCCHHHHEEECCCCCEEEEEEEECCCEEECCCCEEEECCEEEEEECCCCHHHHCCCCHHC
TGGWFEGNIRKLNSLGIMHGEGNGVFAPYRNVTRAEFAALISNALKLPEGDKSFVDINKA
CCCEECCCCEEEECEEEEECCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCC
HPSLHSGIKRSASAGIINGRGGGIFDPNASITREEASIMIDNALQYKGVTGELVALSFRD
CCHHHHHHHHHCCCCEEECCCCCEECCCCCCCCCCCEEEEECCCEECCCCCEEEEEEECC
KHLITYKQPVQRLYSLKVVTGVGNNVFNPKGTTTRGEAAAFLVKMLDASQK
CEEEEHHHHHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNIKKRVIALSTSLVILGSAPLSISAEEINKSHNDQLRIAQGERGTNVLRYSNFNLETPD
CCCHHEEEEEEEEEEEEECCCCEEEHHHHCCCCCCCEEEECCCCCCEEEEEECCCCCCCC
HMQINEAEVSENEEILLEEYKHKHDETEELHKMKEAKKGEVAEDEAISNIEKAFEHVDGR
CCEECHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
NDGSTREMKPVEKRRAIQYSASMTTKKYGTLAGVPFVEWIVPAGNPDIRPANPMKARYIT
CCCCCCCCCHHHHHHHEEHHHHCCHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEE
IHETANTARGANAENHAKYLYKQATEGTFRTASWHFTVDDKQIYQHLPTNENGWHAGDGD
EEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEECHHHHHHHCCCCCCCCCCCCCC
GSGNRESIGIEISVNQDGDYNKALENAKRLAGYLMNKEGISADHIYRHQQWSGKNCPDIL
CCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCCCCCCHHHE
ISRGNWTGFVQGIQWYANVNAQIDSPLQERNELFDSNEYNPAEPNMELVVNGDGINVRSG
EECCCCCCHHEEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCEEECCC
SGLEHHIVRKASNGDRYKVLAVKNGWYKVGNDEWIFYNPSWIKINYNMPHKEVQKPKDDI
CCCHHHHEEECCCCCEEEEEEEECCCEEECCCCEEEECCEEEEEECCCCHHHHCCCCHHC
TGGWFEGNIRKLNSLGIMHGEGNGVFAPYRNVTRAEFAALISNALKLPEGDKSFVDINKA
CCCEECCCCEEEECEEEEECCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCC
HPSLHSGIKRSASAGIINGRGGGIFDPNASITREEASIMIDNALQYKGVTGELVALSFRD
CCHHHHHHHHHCCCCEEECCCCCEECCCCCCCCCCCEEEEECCCEECCCCCEEEEEEECC
KHLITYKQPVQRLYSLKVVTGVGNNVFNPKGTTTRGEAAAFLVKMLDASQK
CEEEEHHHHHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]