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The map label for this gene is aspG [H]

Identifier: 222096604

GI number: 222096604

Start: 2876425

End: 2877429

Strand: Reverse

Name: aspG [H]

Synonym: BCQ_2944

Alternate gene names: 222096604

Gene position: 2877429-2876425 (Counterclockwise)

Preceding gene: 222096607

Following gene: 222096603

Centisome position: 55.18

GC content: 35.02

Gene sequence:

>1005_bases
ATGAAGAAAATTTTATTACTTGCAACTGGTGGGACAATTGCATCTGTGGAGGGGAATGAGGGGCTTGTTCCAGGATTGTC
CGCTGAGGAATTATTGAATTATTTTTCGAAGTCTTCTCGAAACTTAGAAATAGATTGCAAAATCTTAATGAATATTGATA
GTACCAATATGCAACCTGAGCACTGGAAAGAAATAGCTAATGCTGTTTTTAATCACTATGATGACTATGACGGGTTTGTG
ATTACACATGGAACGGATACGTTAGCTTATACATCTTCTGCGTTATCTTATATGCTGCAAGGTTTAAGAAAACCAGTTGT
TTTAACAGGTTCACAAGTACCTATTAGTTTTAAAAAGACAGACGCAAAGAAAAATGTAGCTGATGCTCTTAGGTTTGCCT
GTGAGAGTGTTGGCGGTGTGTTTATTGTTTTTGATAGTAGGGTGATTATTGGGACGAGAGCAGTTAAAATGCGAACAAAA
AGTTATGATGCATTTGAAAGTGTGAATTCTCCATATGTTGCTGAAGTAAATGAAGATGAAGTGAAATATCATTGGAAGCC
AACTTCCTCACATAATGAACTTTCAATAAATACTAATTTATGTACGGATGTTTTTCTTATGAAATTGTATCCAGGTACAA
AGCCAGAGATATTTGATTGTTTAAAAGATTTGTATAAAGGGATTATTATTGAAAGTTTTGGAAACGGTGGATTACCTTTT
GAAGGAAGGAACTTGCTTTCGAAAATTCAAGAGTTGACTGAAATGGGGATTGCTGTAGTGATTACTACTCAATGCCTGGA
AGAAGGAGAAGACATACTTCTCTATGAAGTAGGACGGAAAGTGGCACAACATCAAGTAATATTGTCTGGAGATATGAATA
CGGAAGCCATCATAGCAAAATTGATGTGGGCGCTTGGAAAAACTAATAAATTAGAAGAGATTAAAAAAATTATTGAAGAG
CCTTTAGCCTACGATTTAACAATAAAATCTGATAAGGATTGGTAA

Upstream 100 bases:

>100_bases
TACAAAATCTTGATTAATTCAAATTAAGTTCTATAATGATAAATATAAATACGCATTACGCGAACGATATATCGTTGTTT
ATTTTATGGAGGGGTTGTGT

Downstream 100 bases:

>100_bases
GGACTTCATATGATTTATTTAGGTTTTACATTTAATATGGGGGAGAAATTAAAATGGCAATGACAAACAAGAACGTGCGA
GTGGAGAATGATTTTTTGGG

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase 1; L-asparagine amidohydrolase 1 [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKKILLLATGGTIASVEGNEGLVPGLSAEELLNYFSKSSRNLEIDCKILMNIDSTNMQPEHWKEIANAVFNHYDDYDGFV
ITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKTDAKKNVADALRFACESVGGVFIVFDSRVIIGTRAVKMRTK
SYDAFESVNSPYVAEVNEDEVKYHWKPTSSHNELSINTNLCTDVFLMKLYPGTKPEIFDCLKDLYKGIIIESFGNGGLPF
EGRNLLSKIQELTEMGIAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAKLMWALGKTNKLEEIKKIIEE
PLAYDLTIKSDKDW

Sequences:

>Translated_334_residues
MKKILLLATGGTIASVEGNEGLVPGLSAEELLNYFSKSSRNLEIDCKILMNIDSTNMQPEHWKEIANAVFNHYDDYDGFV
ITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKTDAKKNVADALRFACESVGGVFIVFDSRVIIGTRAVKMRTK
SYDAFESVNSPYVAEVNEDEVKYHWKPTSSHNELSINTNLCTDVFLMKLYPGTKPEIFDCLKDLYKGIIIESFGNGGLPF
EGRNLLSKIQELTEMGIAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAKLMWALGKTNKLEEIKKIIEE
PLAYDLTIKSDKDW
>Mature_334_residues
MKKILLLATGGTIASVEGNEGLVPGLSAEELLNYFSKSSRNLEIDCKILMNIDSTNMQPEHWKEIANAVFNHYDDYDGFV
ITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKTDAKKNVADALRFACESVGGVFIVFDSRVIIGTRAVKMRTK
SYDAFESVNSPYVAEVNEDEVKYHWKPTSSHNELSINTNLCTDVFLMKLYPGTKPEIFDCLKDLYKGIIIESFGNGGLPF
EGRNLLSKIQELTEMGIAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAKLMWALGKTNKLEEIKKIIEE
PLAYDLTIKSDKDW

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=360, Percent_Identity=31.3888888888889, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1788065, Length=336, Percent_Identity=34.5238095238095, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1789327, Length=303, Percent_Identity=25.0825082508251, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI193207372, Length=362, Percent_Identity=30.3867403314917, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17558280, Length=362, Percent_Identity=30.3867403314917, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17558278, Length=362, Percent_Identity=30.3867403314917, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI193207374, Length=362, Percent_Identity=30.3867403314917, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320527, Length=331, Percent_Identity=25.9818731117825, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323189, Length=279, Percent_Identity=27.5985663082437, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6323187, Length=279, Percent_Identity=27.5985663082437, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6323186, Length=279, Percent_Identity=27.5985663082437, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6323184, Length=279, Percent_Identity=27.5985663082437, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24639609, Length=282, Percent_Identity=34.7517730496454, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24645530, Length=266, Percent_Identity=36.4661654135338, Blast_Score=162, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37240; Mature: 37240

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILLLATGGTIASVEGNEGLVPGLSAEELLNYFSKSSRNLEIDCKILMNIDSTNMQPE
CCEEEEEEECCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCHH
HWKEIANAVFNHYDDYDGFVITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKT
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECC
DAKKNVADALRFACESVGGVFIVFDSRVIIGTRAVKMRTKSYDAFESVNSPYVAEVNEDE
CCHHHHHHHHHHHHHHCCCEEEEECCEEEEEEEEEEEECCCCHHHHHCCCCEEEECCCCC
VKYHWKPTSSHNELSINTNLCTDVFLMKLYPGTKPEIFDCLKDLYKGIIIESFGNGGLPF
EEEEECCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHCEEEEECCCCCCCC
EGRNLLSKIQELTEMGIAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAK
CCHHHHHHHHHHHHCCEEEEEEEHHHHCCCCEEEEHHHHHHHHCEEEEECCCCHHHHHHH
LMWALGKTNKLEEIKKIIEEPLAYDLTIKSDKDW
HHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCC
>Mature Secondary Structure
MKKILLLATGGTIASVEGNEGLVPGLSAEELLNYFSKSSRNLEIDCKILMNIDSTNMQPE
CCEEEEEEECCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCHH
HWKEIANAVFNHYDDYDGFVITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKT
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECC
DAKKNVADALRFACESVGGVFIVFDSRVIIGTRAVKMRTKSYDAFESVNSPYVAEVNEDE
CCHHHHHHHHHHHHHHCCCEEEEECCEEEEEEEEEEEECCCCHHHHHCCCCEEEECCCCC
VKYHWKPTSSHNELSINTNLCTDVFLMKLYPGTKPEIFDCLKDLYKGIIIESFGNGGLPF
EEEEECCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHCEEEEECCCCCCCC
EGRNLLSKIQELTEMGIAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAK
CCHHHHHHHHHHHHCCEEEEEEEHHHHCCCCEEEEHHHHHHHHCEEEEECCCCHHHHHHH
LMWALGKTNKLEEIKKIIEEPLAYDLTIKSDKDW
HHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377; 8478318 [H]