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The map label for this gene is ppsA [H]

Identifier: 222096586

GI number: 222096586

Start: 2859063

End: 2861669

Strand: Reverse

Name: ppsA [H]

Synonym: BCQ_2926

Alternate gene names: 222096586

Gene position: 2861669-2859063 (Counterclockwise)

Preceding gene: 222096589

Following gene: 222096584

Centisome position: 54.88

GC content: 38.67

Gene sequence:

>2607_bases
ATGAGTTCTTTCGTTCTCGATTTTCAGGAAATAGAAAAGGGGCAGCTTTCACTCGTCGGCGGAAAAGGTTTGAATTTAGG
GGAGTTATCTAACATTCAAGGTATAGAGGTGCCAGAAGGATTTTGTGTGACAACGGTAGGATATGAAAAGGCCATCGAGC
AAAATGAAAATCTTCAAACTTTGTTGCAGCAGCTAACCAAGTTGAAACTTGAAGACCGAGCTCAAATTGGTGAAATGAGT
AAGGAAATTAGAGAAGTCATTATAGCAGTACAAATCCCTTCTGATGTAGTGGAAGCGGTAGCTCATTATCTCTCACGTTT
TGGAAATGAACATGCGTATGCCGTGCGTTCTAGTGCTACGGCTGAAGATTTACCGTATGCCTCGTTTGCAGGTCAACAAG
ATACGTATTTAAATATTATCGGAAAAGAAGCGATTTTACAGCATGTAAGAAAGTGCTGGGCTTCTTTATTTACAGAGCGA
GCAGTCATGTACCGTATGCAAAATGGTTTTGAACATAATCAAGTTTCTATATGTGTTGTCGTTCAAAAAATGGTTTTCCC
GCAGGCTTCGGGAATTTTATTTACGGCTGATCCGGTTACTTCTAACCGAAAGGTGCTATCAATTGATGCCAGTTTTGGAC
TTGGTGAGGCGTTAGTATCAGGACTGGTCTCTGCCGATAATTATAAAGTAAAAGAAGGTAAAATTACTGAAACGATGATA
GCGACTAAAAAATTAGCTATTTATGCTGTAAAAGAGGGCGGAACAGAGACGAAACAAATTGATTCAGCTCAGCAAAAGAT
TCAAACATTGTCTGAACGACAAATATTACAACTCGCACAGATTGGGAGACAGATTGAAGCTTATTTTGGCTGTCCGCAAG
ATATTGAATGGTGTTTAGCCCGTAATACATTTTATATTGTTCAAAGCAGGCCGATAACGACTTTATATCCAATTCCGGAA
GAAAATGATGGGGAAAATCATGTATATATATCAGTTGGTCACCAACAAATGATGATCGATGCGATGAAACCATTAGGGTT
ATCTTTTTTCCTGTTAACGACAAGTGCCCCAATGCGTAAAGCGGGAGGACGGCTGTTTGTTGATGCTACGCAACAATTAG
CTTCACCTGCTAGCAGAGAGTATTTAATAAATACGTTAGGAAAATCAGATCCACTCGTAAGAGATGCATTAACGACTATA
ATCGAGCGAGATAATTTTATTACATTGTTGCCGGATGATGAAAAAGAAAAGAGTGTTGGTAAAGGTGTGCCACCTGTAAG
CACGCAACCAGAAATCGAAAATGATCCAGCAATCGTTACGGAATTAATAAAGAATAGTGAAGCATCACTTGAAGAGTTAA
AAGAAACTATGCAACTGAAATCAGGAGTGGACGTACTTGATTTTATTTTGGAAGATATTCAGCAATTAAAGAAAGTATTG
TTTAATCCGCAAAGTATAGCAGTTATTATGGCAGGTATGAATGCTTCAACATGGATCAATGAAAAAATGGAGCAATGGCT
GGGCGAAAAGAATGCAGCGGACACCCTTTCTCAATCGGTGCAAAATAATATTACGTCGGAAATGGGTTTAGCATTAATGG
AAGTTGCTGATGTGATTCGGCCGTACGAAGAAGTTATTGCGTATTTACAACATGTAGAAAATGATAGCTTTTTAGATGAA
CTCGTTCAGTTTAAAGGCGGAGAAAAAGCTCGAGAGGCAATAGATGCTTTTCTAAATAAATACGGAATGAGATGTAGCGG
AGAAATCGACATTACGAAAACGCGCTGGAGTGAGAAGCCAACAACCATTATCCCGATGATTTTAAATAATATAAGAGATT
TTGAATATGGTGCAAGTAAACGGAAATTTGAAGAAGGACTGCAAGAAGCTTTGAAAAAAGAAGAAGAGTTAGTAGATCGA
TTGCAGCAATTGCCGGATGGAAAACAAAAAGTAGAAGAAACGAAGCGAATGATTCGCAATATCCGTAATTTTATCGGCTA
TCGTGAATATCCGAAATACGGTATGATTAATCGTTATTTCATATATAAGCAGGCGTTACTAAAAGAAGCTGAGCAACTTG
TGCAGAGCGGTGTCATTCATGAAGTTGATGATATATACTATTTAACTTTTGAAGAACTTCACGAAGTTGTTCGCACGAAG
AAACTAAATTATGAGCTTATTCATAAACAAAAAAATGATTATAAATTATATGAAAAACTAACGCCTCCTCGTATAATGAC
GTCTGATGGAGAAATCATTACAGGTAAATATAAACGAGAAAACCTCCCAGCTGACGCGATTGCAGGTCTGCCTGTTTCTT
CTGGAGTAGTTGAGGGGAGAGCTCGCGTTATTTTAAACATGGAAGAAGCGAATTTAGAAGAGGGAGATATATTAGTTACA
GCTTTTACTGACCCTGGCTGGACGCCATTATTTGTATCTATAAAAGGACTAGTCACTGAAGTCGGCGGACTTATGACTCA
CGGGGCGGTTATTGCACGGGAATATGGATTACCGGCAGTCGTTGGAGTAGAGAACGCTACGAAATTAATAAAAGATGGGC
AACGAATACGAGTACATGGAACAGAAGGGTATATTGAAGTGTTGTAA

Upstream 100 bases:

>100_bases
AACTTAATTATATCATGTAAATCAAATTCGAAATAGACTATTTCTTCTCATTTACTCCTGCTACAATCAAGAACACATGC
ATAGTAAGGAGAGATGTTAT

Downstream 100 bases:

>100_bases
TGAAGTAAAAGATCATCCGCTATAAATAGTGGATGATCTTTTTATTTTGCAATAACTCACTTCTTTAAGCCAGCTGCATA
AAAAGGTCCATGAACTGCGA

Product: phosphoenolpyruvate synthase

Products: AMP; phosphoenolpyruvate; phosphate

Alternate protein names: Putative PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 868; Mature: 867

Protein sequence:

>868_residues
MSSFVLDFQEIEKGQLSLVGGKGLNLGELSNIQGIEVPEGFCVTTVGYEKAIEQNENLQTLLQQLTKLKLEDRAQIGEMS
KEIREVIIAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSATAEDLPYASFAGQQDTYLNIIGKEAILQHVRKCWASLFTER
AVMYRMQNGFEHNQVSICVVVQKMVFPQASGILFTADPVTSNRKVLSIDASFGLGEALVSGLVSADNYKVKEGKITETMI
ATKKLAIYAVKEGGTETKQIDSAQQKIQTLSERQILQLAQIGRQIEAYFGCPQDIEWCLARNTFYIVQSRPITTLYPIPE
ENDGENHVYISVGHQQMMIDAMKPLGLSFFLLTTSAPMRKAGGRLFVDATQQLASPASREYLINTLGKSDPLVRDALTTI
IERDNFITLLPDDEKEKSVGKGVPPVSTQPEIENDPAIVTELIKNSEASLEELKETMQLKSGVDVLDFILEDIQQLKKVL
FNPQSIAVIMAGMNASTWINEKMEQWLGEKNAADTLSQSVQNNITSEMGLALMEVADVIRPYEEVIAYLQHVENDSFLDE
LVQFKGGEKAREAIDAFLNKYGMRCSGEIDITKTRWSEKPTTIIPMILNNIRDFEYGASKRKFEEGLQEALKKEEELVDR
LQQLPDGKQKVEETKRMIRNIRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVIHEVDDIYYLTFEELHEVVRTK
KLNYELIHKQKNDYKLYEKLTPPRIMTSDGEIITGKYKRENLPADAIAGLPVSSGVVEGRARVILNMEEANLEEGDILVT
AFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKLIKDGQRIRVHGTEGYIEVL

Sequences:

>Translated_868_residues
MSSFVLDFQEIEKGQLSLVGGKGLNLGELSNIQGIEVPEGFCVTTVGYEKAIEQNENLQTLLQQLTKLKLEDRAQIGEMS
KEIREVIIAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSATAEDLPYASFAGQQDTYLNIIGKEAILQHVRKCWASLFTER
AVMYRMQNGFEHNQVSICVVVQKMVFPQASGILFTADPVTSNRKVLSIDASFGLGEALVSGLVSADNYKVKEGKITETMI
ATKKLAIYAVKEGGTETKQIDSAQQKIQTLSERQILQLAQIGRQIEAYFGCPQDIEWCLARNTFYIVQSRPITTLYPIPE
ENDGENHVYISVGHQQMMIDAMKPLGLSFFLLTTSAPMRKAGGRLFVDATQQLASPASREYLINTLGKSDPLVRDALTTI
IERDNFITLLPDDEKEKSVGKGVPPVSTQPEIENDPAIVTELIKNSEASLEELKETMQLKSGVDVLDFILEDIQQLKKVL
FNPQSIAVIMAGMNASTWINEKMEQWLGEKNAADTLSQSVQNNITSEMGLALMEVADVIRPYEEVIAYLQHVENDSFLDE
LVQFKGGEKAREAIDAFLNKYGMRCSGEIDITKTRWSEKPTTIIPMILNNIRDFEYGASKRKFEEGLQEALKKEEELVDR
LQQLPDGKQKVEETKRMIRNIRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVIHEVDDIYYLTFEELHEVVRTK
KLNYELIHKQKNDYKLYEKLTPPRIMTSDGEIITGKYKRENLPADAIAGLPVSSGVVEGRARVILNMEEANLEEGDILVT
AFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKLIKDGQRIRVHGTEGYIEVL
>Mature_867_residues
SSFVLDFQEIEKGQLSLVGGKGLNLGELSNIQGIEVPEGFCVTTVGYEKAIEQNENLQTLLQQLTKLKLEDRAQIGEMSK
EIREVIIAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSATAEDLPYASFAGQQDTYLNIIGKEAILQHVRKCWASLFTERA
VMYRMQNGFEHNQVSICVVVQKMVFPQASGILFTADPVTSNRKVLSIDASFGLGEALVSGLVSADNYKVKEGKITETMIA
TKKLAIYAVKEGGTETKQIDSAQQKIQTLSERQILQLAQIGRQIEAYFGCPQDIEWCLARNTFYIVQSRPITTLYPIPEE
NDGENHVYISVGHQQMMIDAMKPLGLSFFLLTTSAPMRKAGGRLFVDATQQLASPASREYLINTLGKSDPLVRDALTTII
ERDNFITLLPDDEKEKSVGKGVPPVSTQPEIENDPAIVTELIKNSEASLEELKETMQLKSGVDVLDFILEDIQQLKKVLF
NPQSIAVIMAGMNASTWINEKMEQWLGEKNAADTLSQSVQNNITSEMGLALMEVADVIRPYEEVIAYLQHVENDSFLDEL
VQFKGGEKAREAIDAFLNKYGMRCSGEIDITKTRWSEKPTTIIPMILNNIRDFEYGASKRKFEEGLQEALKKEEELVDRL
QQLPDGKQKVEETKRMIRNIRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVIHEVDDIYYLTFEELHEVVRTKK
LNYELIHKQKNDYKLYEKLTPPRIMTSDGEIITGKYKRENLPADAIAGLPVSSGVVEGRARVILNMEEANLEEGDILVTA
FTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKLIKDGQRIRVHGTEGYIEVL

Specific function: Might catalyze the phosphorylation of pyruvate to phosphoenolpyruvate (Potential) [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=334, Percent_Identity=34.7305389221557, Blast_Score=181, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17564524, Length=301, Percent_Identity=32.2259136212625, Blast_Score=127, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR002192 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF01326 PPDK_N [H]

EC number: 2.7.9.2

Molecular weight: Translated: 97342; Mature: 97211

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSFVLDFQEIEKGQLSLVGGKGLNLGELSNIQGIEVPEGFCVTTVGYEKAIEQNENLQT
CCCCEECHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHCCCCHHH
LLQQLTKLKLEDRAQIGEMSKEIREVIIAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSAT
HHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCC
AEDLPYASFAGQQDTYLNIIGKEAILQHVRKCWASLFTERAVMYRMQNGFEHNQVSICVV
CCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHH
VQKMVFPQASGILFTADPVTSNRKVLSIDASFGLGEALVSGLVSADNYKVKEGKITETMI
HHHHHCCCCCCEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEECCCCCHHHHH
ATKKLAIYAVKEGGTETKQIDSAQQKIQTLSERQILQLAQIGRQIEAYFGCPQDIEWCLA
HHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RNTFYIVQSRPITTLYPIPEENDGENHVYISVGHQQMMIDAMKPLGLSFFLLTTSAPMRK
CCEEEEEECCCCEEEECCCCCCCCCCEEEEEECCHHHHHHHHCCCCCEEEEEECCCCHHH
AGGRLFVDATQQLASPASREYLINTLGKSDPLVRDALTTIIERDNFITLLPDDEKEKSVG
CCCEEEEECHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHC
KGVPPVSTQPEIENDPAIVTELIKNSEASLEELKETMQLKSGVDVLDFILEDIQQLKKVL
CCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FNPQSIAVIMAGMNASTWINEKMEQWLGEKNAADTLSQSVQNNITSEMGLALMEVADVIR
CCCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PYEEVIAYLQHVENDSFLDELVQFKGGEKAREAIDAFLNKYGMRCSGEIDITKTRWSEKP
CHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
TTIIPMILNNIRDFEYGASKRKFEEGLQEALKKEEELVDRLQQLPDGKQKVEETKRMIRN
CEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVIHEVDDIYYLTFEELHEVVRTK
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHH
KLNYELIHKQKNDYKLYEKLTPPRIMTSDGEIITGKYKRENLPADAIAGLPVSSGVVEGR
CCCHHHHHCCCCCHHHHHHCCCCCEEECCCCEEECCCCCCCCCHHHHCCCCCCCCHHCCC
ARVILNMEEANLEEGDILVTAFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAV
EEEEEEEHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCCEE
VGVENATKLIKDGQRIRVHGTEGYIEVL
EECHHHHHHHHCCCEEEEECCCCEEEEC
>Mature Secondary Structure 
SSFVLDFQEIEKGQLSLVGGKGLNLGELSNIQGIEVPEGFCVTTVGYEKAIEQNENLQT
CCCEECHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHCCCCHHH
LLQQLTKLKLEDRAQIGEMSKEIREVIIAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSAT
HHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCC
AEDLPYASFAGQQDTYLNIIGKEAILQHVRKCWASLFTERAVMYRMQNGFEHNQVSICVV
CCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHH
VQKMVFPQASGILFTADPVTSNRKVLSIDASFGLGEALVSGLVSADNYKVKEGKITETMI
HHHHHCCCCCCEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEECCCCCHHHHH
ATKKLAIYAVKEGGTETKQIDSAQQKIQTLSERQILQLAQIGRQIEAYFGCPQDIEWCLA
HHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RNTFYIVQSRPITTLYPIPEENDGENHVYISVGHQQMMIDAMKPLGLSFFLLTTSAPMRK
CCEEEEEECCCCEEEECCCCCCCCCCEEEEEECCHHHHHHHHCCCCCEEEEEECCCCHHH
AGGRLFVDATQQLASPASREYLINTLGKSDPLVRDALTTIIERDNFITLLPDDEKEKSVG
CCCEEEEECHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHC
KGVPPVSTQPEIENDPAIVTELIKNSEASLEELKETMQLKSGVDVLDFILEDIQQLKKVL
CCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FNPQSIAVIMAGMNASTWINEKMEQWLGEKNAADTLSQSVQNNITSEMGLALMEVADVIR
CCCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PYEEVIAYLQHVENDSFLDELVQFKGGEKAREAIDAFLNKYGMRCSGEIDITKTRWSEKP
CHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
TTIIPMILNNIRDFEYGASKRKFEEGLQEALKKEEELVDRLQQLPDGKQKVEETKRMIRN
CEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVIHEVDDIYYLTFEELHEVVRTK
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHH
KLNYELIHKQKNDYKLYEKLTPPRIMTSDGEIITGKYKRENLPADAIAGLPVSSGVVEGR
CCCHHHHHCCCCCHHHHHHCCCCCEEECCCCEEECCCCCCCCCHHHHCCCCCCCCHHCCC
ARVILNMEEANLEEGDILVTAFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAV
EEEEEEEHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCCEE
VGVENATKLIKDGQRIRVHGTEGYIEVL
EECHHHHHHHHCCCEEEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10.5 {phosphate}} 0.028 {ATP}} 0.083 {pyruvate}} [C]

Substrates: ATP; pyruvate; H2O

Specific reaction: ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate

General reaction: Phospho group transfer [C]

Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 7704256 [H]