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The map label for this gene is aacA-aphD [H]

Identifier: 222096450

GI number: 222096450

Start: 2723969

End: 2725048

Strand: Reverse

Name: aacA-aphD [H]

Synonym: BCQ_2790

Alternate gene names: 222096450

Gene position: 2725048-2723969 (Counterclockwise)

Preceding gene: 222096451

Following gene: 222096448

Centisome position: 52.26

GC content: 37.69

Gene sequence:

>1080_bases
ATGCTATTTCAAAAAGATAATGTATCGGTTAGGTATGTAGTAGAAGAGGATGCACCAACCATTTCAAAATGGTTAACAGA
CCCAGAAGTGTTGCAGTATTATGAAGGACGAGATAATCCGCAATCAGTAGAAATGGTACTGGATCATTTTATACATAATC
CAAATAGTCATGAAAAAAGATGCTTAATAGAGTTTGATGGTATGCCGATCGGTTATATTCAAATGTATCCAGTTGATTCA
GAGTGGAAAACTTTATATGACTATGAAGCATCACAAAATGTATGGGGAATGGATCAATTTATCGGAGAACCCGCTTATTG
GGAAAAGGGCATTGGAACTAAACTTGTTCAGGCAGCAATTACATATATTATGGAGAACACGGGAGCAAAAGCAATCGCAA
TGGACCCGAAAGTAAATAATGAACGTGCAATAAAGTGTTATGAAAAATGCGGATTTAAAAAGGTAAAGATTTTAAAAGAA
CATGAACTGCATGAAGGGGTATTAGAAGATTGTTGGATGATGGAATATAAACAAAGGGAGCTGCGCGATATGAAAAAAGC
ATTATTAGTAATCGATGTGCAAGCAGGTATGTATACAGCAAAAATGCCGGTACATAATGGGGAAAAGTTTTTACAAACAT
TACAAGAGCTTATTGAGAAATGCCGTTCAAATGATATTCCGGTAATTTATGTGCAACATAATGGACGGAAAGACCATCCG
TTAGAAAAAGGTACAGACGGGTGGAAAATCCATGCGGCAATTGCTCCACTAGAAGGGGAGTGTGTGGTTGAAAAAACGAC
TCCAGATTCATTCCACAAGACGAACCTAAAAGAAGTATTGCAAGACAAGGGAATCGATCATGTTATCATTTCAGGAATGC
AAACGGAGTACTGTGTGGATACGACAACGCGAAGAGCGTGTAGTGAAGGGTATAAAGTAACGTTAGTGAGCGATGCACAT
AGTACGTTTGATACAGAGGTGTTACGTGCTGAAGACATTGTGAAGCATCATAACGTAGTGTTTGGAGCGTTTGCTAATGT
TGTTACGTTAAAAGATTTGAAAGAAACTGTCTCTAAATAA

Upstream 100 bases:

>100_bases
ACAGCTTTTTTTGCGATTACATTGTTACCATACGCATTAATAAGTGCAATTAAAGATTTCACTACTAGAAAAAATATAAA
TAATTAAAAAGAGGTATTAT

Downstream 100 bases:

>100_bases
GAGGCAGTTTTTTATTAGAGGAAATCGTGTCAAATAATGTCGGTAAGTCGATATCCTTTGTCGAAACGTCGATATATTGC
AATAATCGTTGATATATTTG

Product: acetyltransferase, gnat family

Products: NA

Alternate protein names: 6'-aminoglycoside N-acetyltransferase; AAC(6'); 2''-aminoglycoside phosphotransferase; APH(2'') [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MLFQKDNVSVRYVVEEDAPTISKWLTDPEVLQYYEGRDNPQSVEMVLDHFIHNPNSHEKRCLIEFDGMPIGYIQMYPVDS
EWKTLYDYEASQNVWGMDQFIGEPAYWEKGIGTKLVQAAITYIMENTGAKAIAMDPKVNNERAIKCYEKCGFKKVKILKE
HELHEGVLEDCWMMEYKQRELRDMKKALLVIDVQAGMYTAKMPVHNGEKFLQTLQELIEKCRSNDIPVIYVQHNGRKDHP
LEKGTDGWKIHAAIAPLEGECVVEKTTPDSFHKTNLKEVLQDKGIDHVIISGMQTEYCVDTTTRRACSEGYKVTLVSDAH
STFDTEVLRAEDIVKHHNVVFGAFANVVTLKDLKETVSK

Sequences:

>Translated_359_residues
MLFQKDNVSVRYVVEEDAPTISKWLTDPEVLQYYEGRDNPQSVEMVLDHFIHNPNSHEKRCLIEFDGMPIGYIQMYPVDS
EWKTLYDYEASQNVWGMDQFIGEPAYWEKGIGTKLVQAAITYIMENTGAKAIAMDPKVNNERAIKCYEKCGFKKVKILKE
HELHEGVLEDCWMMEYKQRELRDMKKALLVIDVQAGMYTAKMPVHNGEKFLQTLQELIEKCRSNDIPVIYVQHNGRKDHP
LEKGTDGWKIHAAIAPLEGECVVEKTTPDSFHKTNLKEVLQDKGIDHVIISGMQTEYCVDTTTRRACSEGYKVTLVSDAH
STFDTEVLRAEDIVKHHNVVFGAFANVVTLKDLKETVSK
>Mature_359_residues
MLFQKDNVSVRYVVEEDAPTISKWLTDPEVLQYYEGRDNPQSVEMVLDHFIHNPNSHEKRCLIEFDGMPIGYIQMYPVDS
EWKTLYDYEASQNVWGMDQFIGEPAYWEKGIGTKLVQAAITYIMENTGAKAIAMDPKVNNERAIKCYEKCGFKKVKILKE
HELHEGVLEDCWMMEYKQRELRDMKKALLVIDVQAGMYTAKMPVHNGEKFLQTLQELIEKCRSNDIPVIYVQHNGRKDHP
LEKGTDGWKIHAAIAPLEGECVVEKTTPDSFHKTNLKEVLQDKGIDHVIISGMQTEYCVDTTTRRACSEGYKVTLVSDAH
STFDTEVLRAEDIVKHHNVVFGAFANVVTLKDLKETVSK

Specific function: Resistance to gentamicin, tobramycin, and kanamycin. Tobramycin and kanamycin resistance is due to the ACC activity, specified by N-terminal region, and the gentamicin resistance is due to the APH activity encoded by the C-terminal region of the protein [

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR002575
- InterPro:   IPR011009 [H]

Pfam domain/function: PF00583 Acetyltransf_1; PF01636 APH [H]

EC number: NA

Molecular weight: Translated: 41198; Mature: 41198

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFQKDNVSVRYVVEEDAPTISKWLTDPEVLQYYEGRDNPQSVEMVLDHFIHNPNSHEKR
CCCCCCCCEEEEEECCCCCHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCE
CLIEFDGMPIGYIQMYPVDSEWKTLYDYEASQNVWGMDQFIGEPAYWEKGIGTKLVQAAI
EEEEECCCCEEEEEEEECCCCCCEEEECCCCCCCCCHHHHHCCCCHHHCCCCHHHHHHHH
TYIMENTGAKAIAMDPKVNNERAIKCYEKCGFKKVKILKEHELHEGVLEDCWMMEYKQRE
HHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
LRDMKKALLVIDVQAGMYTAKMPVHNGEKFLQTLQELIEKCRSNDIPVIYVQHNGRKDHP
HHHHHHEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
LEKGTDGWKIHAAIAPLEGECVVEKTTPDSFHKTNLKEVLQDKGIDHVIISGMQTEYCVD
CCCCCCCEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHC
TTTRRACSEGYKVTLVSDAHSTFDTEVLRAEDIVKHHNVVFGAFANVVTLKDLKETVSK
HHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLFQKDNVSVRYVVEEDAPTISKWLTDPEVLQYYEGRDNPQSVEMVLDHFIHNPNSHEKR
CCCCCCCCEEEEEECCCCCHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCE
CLIEFDGMPIGYIQMYPVDSEWKTLYDYEASQNVWGMDQFIGEPAYWEKGIGTKLVQAAI
EEEEECCCCEEEEEEEECCCCCCEEEECCCCCCCCCHHHHHCCCCHHHCCCCHHHHHHHH
TYIMENTGAKAIAMDPKVNNERAIKCYEKCGFKKVKILKEHELHEGVLEDCWMMEYKQRE
HHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
LRDMKKALLVIDVQAGMYTAKMPVHNGEKFLQTLQELIEKCRSNDIPVIYVQHNGRKDHP
HHHHHHEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
LEKGTDGWKIHAAIAPLEGECVVEKTTPDSFHKTNLKEVLQDKGIDHVIISGMQTEYCVD
CCCCCCCEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHC
TTTRRACSEGYKVTLVSDAHSTFDTEVLRAEDIVKHHNVVFGAFANVVTLKDLKETVSK
HHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3015884; 12663927 [H]