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The map label for this gene is cwlA [H]

Identifier: 222096319

GI number: 222096319

Start: 2601975

End: 2602805

Strand: Reverse

Name: cwlA [H]

Synonym: BCQ_2659

Alternate gene names: 222096319

Gene position: 2602805-2601975 (Counterclockwise)

Preceding gene: 222096320

Following gene: 222096316

Centisome position: 49.92

GC content: 36.1

Gene sequence:

>831_bases
ATGGGTTATATTGTAGATATTTCGAAATGGAATGGCGACATTAACTGGGATATCGCAGCACCACAATTAGATTTTGTAAT
TGCACGTGTGCAAGATGGTTCAAATTATGTAGATCCTTTATATAAAAATTATGTGCAAGCAATGAAAGCAAGAAATATTC
CGTTTGGTAATTATGCGTTCTGTCGTTTCGTTTCAGTAGAGGATGCACGGATAGAGGCGAGAGACTTTTGGAACCGTGGT
GATAAAAATGCAACAGTTTGGGTAGCTGACGTAGAAGTAAAAACAATGAACGATATGAGAGCGGGTGCACAAGCTTTTAT
TGATGAATTACGCAGATTAGGAGCTAAGAAGGTTGGTTTATATATTGCTCATCATATGTATGATCCATTTGGAATGGCAA
ATGTCAATTGTGATTTTGTATGGATTCCTCGTTATGGTGGTAACAAACCAGCCTACCCATGTGATATTTGGCAATACACA
GAAACAGGGAATGTAGCTGGCATAGGAAAATGTGATTTAAATAAGTTAATGGGAAATAAGCCGTTAGTTTGGTTTACTAA
TCAAACTATTGAACAATCGACAAAACAAGAAGGAGTTGGAATTATAGTGAATAAATTTAGTAAAGTTGTTACGTATGAAT
TTGGTACAGCGTTAGTAGTAGAAATGTTAGGAATGATGGATGCTCTGGGGTATGAATCTCGTATTATCTCATATGGAGAT
AAACAAGGATTAGTTAGATTTGAAACAGCATATCGCCAAGGGAATGAATTAGATCGAGCGACAGCGTGGTTAGATGCTAA
AGGACTTAAATATTTTTATACAAAAGAGTAG

Upstream 100 bases:

>100_bases
AATATTACGCAATAATACACTACCTTTCATACTATTTATTAGTTGATAATAGCAAAAAAACAAGCTTTGAATATAATAAT
GAAAAAGGAGATGTGAAATA

Downstream 100 bases:

>100_bases
TTTGCTAAATAAAAAGCCGTCATGTGACGGTTTTTTATTTTTATGTAATCTTTTAACTCTATGTAAAACAAAAGCGAAAT
GCCATTGTAGGCATGAAGAG

Product: peptidase, u32 family

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MGYIVDISKWNGDINWDIAAPQLDFVIARVQDGSNYVDPLYKNYVQAMKARNIPFGNYAFCRFVSVEDARIEARDFWNRG
DKNATVWVADVEVKTMNDMRAGAQAFIDELRRLGAKKVGLYIAHHMYDPFGMANVNCDFVWIPRYGGNKPAYPCDIWQYT
ETGNVAGIGKCDLNKLMGNKPLVWFTNQTIEQSTKQEGVGIIVNKFSKVVTYEFGTALVVEMLGMMDALGYESRIISYGD
KQGLVRFETAYRQGNELDRATAWLDAKGLKYFYTKE

Sequences:

>Translated_276_residues
MGYIVDISKWNGDINWDIAAPQLDFVIARVQDGSNYVDPLYKNYVQAMKARNIPFGNYAFCRFVSVEDARIEARDFWNRG
DKNATVWVADVEVKTMNDMRAGAQAFIDELRRLGAKKVGLYIAHHMYDPFGMANVNCDFVWIPRYGGNKPAYPCDIWQYT
ETGNVAGIGKCDLNKLMGNKPLVWFTNQTIEQSTKQEGVGIIVNKFSKVVTYEFGTALVVEMLGMMDALGYESRIISYGD
KQGLVRFETAYRQGNELDRATAWLDAKGLKYFYTKE
>Mature_275_residues
GYIVDISKWNGDINWDIAAPQLDFVIARVQDGSNYVDPLYKNYVQAMKARNIPFGNYAFCRFVSVEDARIEARDFWNRGD
KNATVWVADVEVKTMNDMRAGAQAFIDELRRLGAKKVGLYIAHHMYDPFGMANVNCDFVWIPRYGGNKPAYPCDIWQYTE
TGNVAGIGKCDLNKLMGNKPLVWFTNQTIEQSTKQEGVGIIVNKFSKVVTYEFGTALVVEMLGMMDALGYESRIISYGDK
QGLVRFETAYRQGNELDRATAWLDAKGLKYFYTKE

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation [H]

COG id: COG3757

COG function: function code M; Lyzozyme M1 (1,4-beta-N-acetylmuramidase)

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021976
- InterPro:   IPR002502 [H]

Pfam domain/function: PF12123 Amidase02_C; PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 31416; Mature: 31285

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYIVDISKWNGDINWDIAAPQLDFVIARVQDGSNYVDPLYKNYVQAMKARNIPFGNYAF
CCEEEEEECCCCCEEEEEECCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEE
CRFVSVEDARIEARDFWNRGDKNATVWVADVEVKTMNDMRAGAQAFIDELRRLGAKKVGL
EEEEECCCCEEHHHHHHCCCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHH
YIAHHMYDPFGMANVNCDFVWIPRYGGNKPAYPCDIWQYTETGNVAGIGKCDLNKLMGNK
EEHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEECCCCCEEEECCCHHHHHHCCC
PLVWFTNQTIEQSTKQEGVGIIVNKFSKVVTYEFGTALVVEMLGMMDALGYESRIISYGD
CEEEEECCHHHHHHHHCCCEEEEECHHHHEEEECHHHHHHHHHHHHHHCCCCHHEEECCC
KQGLVRFETAYRQGNELDRATAWLDAKGLKYFYTKE
CCCCEEEEHHHHCCCCHHHHHHEECCCCCEEEEECC
>Mature Secondary Structure 
GYIVDISKWNGDINWDIAAPQLDFVIARVQDGSNYVDPLYKNYVQAMKARNIPFGNYAF
CEEEEEECCCCCEEEEEECCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEE
CRFVSVEDARIEARDFWNRGDKNATVWVADVEVKTMNDMRAGAQAFIDELRRLGAKKVGL
EEEEECCCCEEHHHHHHCCCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHH
YIAHHMYDPFGMANVNCDFVWIPRYGGNKPAYPCDIWQYTETGNVAGIGKCDLNKLMGNK
EEHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEECCCCCEEEECCCHHHHHHCCC
PLVWFTNQTIEQSTKQEGVGIIVNKFSKVVTYEFGTALVVEMLGMMDALGYESRIISYGD
CEEEEECCHHHHHHHHCCCEEEEECHHHHEEEECHHHHHHHHHHHHHHCCCCHHEEECCC
KQGLVRFETAYRQGNELDRATAWLDAKGLKYFYTKE
CCCCEEEEHHHHCCCCHHHHHHEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3070348 [H]