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The map label for this gene is pulA [H]

Identifier: 222096238

GI number: 222096238

Start: 2533179

End: 2535731

Strand: Reverse

Name: pulA [H]

Synonym: BCQ_2578

Alternate gene names: 222096238

Gene position: 2535731-2533179 (Counterclockwise)

Preceding gene: 222096239

Following gene: 222096237

Centisome position: 48.63

GC content: 34.0

Gene sequence:

>2553_bases
ATGACAAAAAGATTAATTAACAAATCAGTTTTATTACTTACTATAATTGTTATGTTCGCATCTATTTTCTCTATTCAAAG
TGTAAAGGCAGTTTCAAATTCGAAAACAACTGAAGTTATCATTCATTACAAAGAGCAGCTTGGAAATACAAAAGACTGGA
ATCTTTGGTTATGGGGAGAAAATGCAAATGGTACATCTTATGAATTTACTGGTGAAGATGAATTTGGAAAGTACGCTAAG
ATTAATATAGATGGTGACTATAATCGTGTAGGATTCATCATTCGTACAAATGAGTGGGAAAAAGATGGTGGGGATCGTTG
GATTGAAAATATAAAGGACGGTCGTGCTGAAGTATGGATTCTTTCTGGTGATGAAAAGGTATATAACGCTAAGCCTTCTT
CGGATCTATCAATTCAAAAAGCAACTATTGATAGTTTCAATGAAATCACTGTAACGACGAATGCTCCATTTCATATTAAG
GAACAGAAAATCGAAATTGAAGGTATTAAAATTAAGAATATTACTCCTTATGATATAAACAGTGGAGATATTACGAATAA
GGTGAAAATAATTACGGAACAGAAAATTGATTTAAAACAAACATACAAAGTTAAAATAGAAAACTTAGCTGATACGAATA
CTGAAATTGGTAAAGTCATTCGTAGTGAGGAATTTGATCATTTATTTTATTATGGTGGTAACGATTTAGGAAATACATAT
ACCGTGCAACATACAAAGTTCCGTGTATGGGCTCCTACTGCGAGTGAAGCGAAGTTAGTTACATATAAAAAATGGAACGA
TAAAATAGGTACTGAAATAAATATGCAACAAGGTGAAAAGGGCACCTGGAAAGCAGAACTTAAAGGGAATCAAAAAGGTC
TCTATTATACGTATAAGGTAAAAATTGGTGATAAGTGGAAAGAAGCAGTTGATCCGTATGCACGTGCTGCTTCTGTAAAT
GGAGATAAAGGTGCGGTTATTGATTTAGAAGAAACAAATCCGAAAAAATGGAACACGAACAAAAAGCCTAAATTAAAAAA
TCCGGAAGATGCTATTATTTATGAATTGCATGTACGTGATCTTTCTATTCAGCCCGAAAGTGGCATTAAACAGAAAGGAA
AATATTTAGGTGTAACAGAAAAAGGAACAAGAGGGCCTGAAGATGTGAAAACAGGACTTGATCATATAAAAGATCTTGGT
GTTACACACGTTCAGCTTTTGCCGATATTTGATTATGCTTCAGTAAATGAAGAGAATGTAAACGAACCACAATATAACTG
GGGATATGATCCAAAAAACTTTAACGTACCAGAGGGGTCCTATTCTACAAATCCATACGAGCCAACTGTTCGAATAACTG
AATTAAAACAAATGATTCAAACACTACATGATAATAATCTTCGTGTTGTTATGGATGTAGTTTATAACCATATGTATAAT
GCTGCTGAATCTAATTTTCATAAGTTAGTTCCCGGTTATTATTATCGTTACAATGAAGATGGAACATTTGCAAATGGAAC
CGGTGTAGGAAATGATACGGCTTCAGAAAGAAAAATGATGAGGAAATTTATGATAGATTCCGTCACATATTGGGCAAAAG
AATACAATTTAGACGGATTCCGTTTTGATTTAATGGGTATTCATGATTATGAAACGATGAATGAAATACGTAAAGCTGTT
AATCAAATTGACCCTTCTATTATACTGCATGGAGAAGGTTGGGATTTAAATACACCTCTTGCAGCAGAGTTGAAAGCAAA
TCAAAAAAATGCAGAGAAAATGAAAGGGATTGCTCATTTTAACGATAATATACGTGATGGATTAAAAGGTAGTGTATTTG
AAGAGAAAGAAAATGGGTTTGTAAATGGAAAGGAACACATGGAAGACCGTATTAAAAAAGGTATTACCGCAGGCATTGAC
TATGATACAAATTCGTCGACTTACCAAGATCCAGAGCAGGTACTAACGTATGTGGAAGCACATGATAATCATACATTATG
GGATAAACTTGAGTTGACTAATCCAAGTGACAGTGAAGAAGTGCGAAAACAAATGCATAAATTATCTTCTTCGATTTTAT
TAACATCACAAGGTATTCCGTTCTTACATGCGGGACAAGAGTTTATGCGCACGAAATATGGTGATCATAACAGTTACAAA
TCTCCAGATTCAATTAACCAGATGGACTGGTTGCGCCGTGCAACGTTTAATAATGAAGTGGATTATATGAAGGGTTTAAT
AGAATTACGCAAAAAGTACTCAGCTTTTCGAATGACATCTGCAGAGCAAATAAAAACACATGTATCATTTATTGATGCTC
CGAAAAATACTATAGCTTATACAATAGAGGGGAATAAAAATGAATACTTTACGGTGGCTCACAATGCAAATAGAGAAACT
GTTGAAATAACACTTCCATCGAAAGGTCCTTGGAAAGTACTAGTAGACGGGAAGCAGGCGGGAAGTAAATCCCTTTATGT
TGTGCATGACAATAAAATTAAAGTTCCTGCGTTAAGTTCATTTGTTTTAAAAACAGAGAAACCGATTAAATAA

Upstream 100 bases:

>100_bases
GGGAGGCAAAAGAGATTTCTAGATTAATTTAAATTGTGTAATGTAGTACGGGCATAATAAATCGTCCGTACTATTTAAAG
AAAGGGTGTATGTAGTGCAA

Downstream 100 bases:

>100_bases
ATGCCAAAAGCGAACGAAAGATTGTTTTCGCTTCGCTTTTAAAGTTAAAATAGTATAATTTCACATACTGTAATTGTATT
ACAATTTAATCAATAAAAAA

Product: pullulanase

Products: NA

Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]

Number of amino acids: Translated: 850; Mature: 849

Protein sequence:

>850_residues
MTKRLINKSVLLLTIIVMFASIFSIQSVKAVSNSKTTEVIIHYKEQLGNTKDWNLWLWGENANGTSYEFTGEDEFGKYAK
INIDGDYNRVGFIIRTNEWEKDGGDRWIENIKDGRAEVWILSGDEKVYNAKPSSDLSIQKATIDSFNEITVTTNAPFHIK
EQKIEIEGIKIKNITPYDINSGDITNKVKIITEQKIDLKQTYKVKIENLADTNTEIGKVIRSEEFDHLFYYGGNDLGNTY
TVQHTKFRVWAPTASEAKLVTYKKWNDKIGTEINMQQGEKGTWKAELKGNQKGLYYTYKVKIGDKWKEAVDPYARAASVN
GDKGAVIDLEETNPKKWNTNKKPKLKNPEDAIIYELHVRDLSIQPESGIKQKGKYLGVTEKGTRGPEDVKTGLDHIKDLG
VTHVQLLPIFDYASVNEENVNEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMIQTLHDNNLRVVMDVVYNHMYN
AAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERKMMRKFMIDSVTYWAKEYNLDGFRFDLMGIHDYETMNEIRKAV
NQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKGIAHFNDNIRDGLKGSVFEEKENGFVNGKEHMEDRIKKGITAGID
YDTNSSTYQDPEQVLTYVEAHDNHTLWDKLELTNPSDSEEVRKQMHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNSYK
SPDSINQMDWLRRATFNNEVDYMKGLIELRKKYSAFRMTSAEQIKTHVSFIDAPKNTIAYTIEGNKNEYFTVAHNANRET
VEITLPSKGPWKVLVDGKQAGSKSLYVVHDNKIKVPALSSFVLKTEKPIK

Sequences:

>Translated_850_residues
MTKRLINKSVLLLTIIVMFASIFSIQSVKAVSNSKTTEVIIHYKEQLGNTKDWNLWLWGENANGTSYEFTGEDEFGKYAK
INIDGDYNRVGFIIRTNEWEKDGGDRWIENIKDGRAEVWILSGDEKVYNAKPSSDLSIQKATIDSFNEITVTTNAPFHIK
EQKIEIEGIKIKNITPYDINSGDITNKVKIITEQKIDLKQTYKVKIENLADTNTEIGKVIRSEEFDHLFYYGGNDLGNTY
TVQHTKFRVWAPTASEAKLVTYKKWNDKIGTEINMQQGEKGTWKAELKGNQKGLYYTYKVKIGDKWKEAVDPYARAASVN
GDKGAVIDLEETNPKKWNTNKKPKLKNPEDAIIYELHVRDLSIQPESGIKQKGKYLGVTEKGTRGPEDVKTGLDHIKDLG
VTHVQLLPIFDYASVNEENVNEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMIQTLHDNNLRVVMDVVYNHMYN
AAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERKMMRKFMIDSVTYWAKEYNLDGFRFDLMGIHDYETMNEIRKAV
NQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKGIAHFNDNIRDGLKGSVFEEKENGFVNGKEHMEDRIKKGITAGID
YDTNSSTYQDPEQVLTYVEAHDNHTLWDKLELTNPSDSEEVRKQMHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNSYK
SPDSINQMDWLRRATFNNEVDYMKGLIELRKKYSAFRMTSAEQIKTHVSFIDAPKNTIAYTIEGNKNEYFTVAHNANRET
VEITLPSKGPWKVLVDGKQAGSKSLYVVHDNKIKVPALSSFVLKTEKPIK
>Mature_849_residues
TKRLINKSVLLLTIIVMFASIFSIQSVKAVSNSKTTEVIIHYKEQLGNTKDWNLWLWGENANGTSYEFTGEDEFGKYAKI
NIDGDYNRVGFIIRTNEWEKDGGDRWIENIKDGRAEVWILSGDEKVYNAKPSSDLSIQKATIDSFNEITVTTNAPFHIKE
QKIEIEGIKIKNITPYDINSGDITNKVKIITEQKIDLKQTYKVKIENLADTNTEIGKVIRSEEFDHLFYYGGNDLGNTYT
VQHTKFRVWAPTASEAKLVTYKKWNDKIGTEINMQQGEKGTWKAELKGNQKGLYYTYKVKIGDKWKEAVDPYARAASVNG
DKGAVIDLEETNPKKWNTNKKPKLKNPEDAIIYELHVRDLSIQPESGIKQKGKYLGVTEKGTRGPEDVKTGLDHIKDLGV
THVQLLPIFDYASVNEENVNEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMIQTLHDNNLRVVMDVVYNHMYNA
AESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERKMMRKFMIDSVTYWAKEYNLDGFRFDLMGIHDYETMNEIRKAVN
QIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKGIAHFNDNIRDGLKGSVFEEKENGFVNGKEHMEDRIKKGITAGIDY
DTNSSTYQDPEQVLTYVEAHDNHTLWDKLELTNPSDSEEVRKQMHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNSYKS
PDSINQMDWLRRATFNNEVDYMKGLIELRKKYSAFRMTSAEQIKTHVSFIDAPKNTIAYTIEGNKNEYFTVAHNANRETV
EITLPSKGPWKVLVDGKQAGSKSLYVVHDNKIKVPALSSFVLKTEKPIK

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=664, Percent_Identity=21.8373493975904, Blast_Score=117, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR005323
- InterPro:   IPR011840 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; PF03714 PUD [H]

EC number: =3.2.1.41 [H]

Molecular weight: Translated: 97173; Mature: 97042

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRLINKSVLLLTIIVMFASIFSIQSVKAVSNSKTTEVIIHYKEQLGNTKDWNLWLWGE
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCCCCCCEEEEEEC
NANGTSYEFTGEDEFGKYAKINIDGDYNRVGFIIRTNEWEKDGGDRWIENIKDGRAEVWI
CCCCCEEEECCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEE
LSGDEKVYNAKPSSDLSIQKATIDSFNEITVTTNAPFHIKEQKIEIEGIKIKNITPYDIN
EECCCEEECCCCCCCCEEEEEECCCCCEEEEEECCCEEEEEEEEEEEEEEECCCCCCCCC
SGDITNKVKIITEQKIDLKQTYKVKIENLADTNTEIGKVIRSEEFDHLFYYGGNDLGNTY
CCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCCEE
TVQHTKFRVWAPTASEAKLVTYKKWNDKIGTEINMQQGEKGTWKAELKGNQKGLYYTYKV
EEEEEEEEEECCCCCCCEEEEEEECCCCCCCEEECCCCCCCCEEEEECCCCCCEEEEEEE
KIGDKWKEAVDPYARAASVNGDKGAVIDLEETNPKKWNTNKKPKLKNPEDAIIYELHVRD
EECCHHHHHHCHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEE
LSIQPESGIKQKGKYLGVTEKGTRGPEDVKTGLDHIKDLGVTHVQLLPIFDYASVNEENV
EEECCCCCHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCC
NEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMIQTLHDNNLRVVMDVVYNHMYN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCEEHHHHHHHHHHH
AAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERKMMRKFMIDSVTYWAKEYNLDGF
HHHCCHHHCCCCEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
RFDLMGIHDYETMNEIRKAVNQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKGIAHF
EEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHEECCCCCHHHHHHHHHHC
NDNIRDGLKGSVFEEKENGFVNGKEHMEDRIKKGITAGIDYDTNSSTYQDPEQVLTYVEA
CCCHHCCCCCCCEECCCCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHHHC
HDNHTLWDKLELTNPSDSEEVRKQMHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNSYK
CCCCEEEEEEEECCCCCHHHHHHHHHHHHCEEEEEECCCCCEECCHHHHHHHCCCCCCCC
SPDSINQMDWLRRATFNNEVDYMKGLIELRKKYSAFRMTSAEQIKTHVSFIDAPKNTIAY
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEECCCCEEEE
TIEGNKNEYFTVAHNANRETVEITLPSKGPWKVLVDGKQAGSKSLYVVHDNKIKVPALSS
EEECCCCEEEEEEECCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEEECCEECCCCHHH
FVLKTEKPIK
HHCCCCCCCC
>Mature Secondary Structure 
TKRLINKSVLLLTIIVMFASIFSIQSVKAVSNSKTTEVIIHYKEQLGNTKDWNLWLWGE
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCCCCCCEEEEEEC
NANGTSYEFTGEDEFGKYAKINIDGDYNRVGFIIRTNEWEKDGGDRWIENIKDGRAEVWI
CCCCCEEEECCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEE
LSGDEKVYNAKPSSDLSIQKATIDSFNEITVTTNAPFHIKEQKIEIEGIKIKNITPYDIN
EECCCEEECCCCCCCCEEEEEECCCCCEEEEEECCCEEEEEEEEEEEEEEECCCCCCCCC
SGDITNKVKIITEQKIDLKQTYKVKIENLADTNTEIGKVIRSEEFDHLFYYGGNDLGNTY
CCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCCEE
TVQHTKFRVWAPTASEAKLVTYKKWNDKIGTEINMQQGEKGTWKAELKGNQKGLYYTYKV
EEEEEEEEEECCCCCCCEEEEEEECCCCCCCEEECCCCCCCCEEEEECCCCCCEEEEEEE
KIGDKWKEAVDPYARAASVNGDKGAVIDLEETNPKKWNTNKKPKLKNPEDAIIYELHVRD
EECCHHHHHHCHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEE
LSIQPESGIKQKGKYLGVTEKGTRGPEDVKTGLDHIKDLGVTHVQLLPIFDYASVNEENV
EEECCCCCHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCC
NEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMIQTLHDNNLRVVMDVVYNHMYN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCEEHHHHHHHHHHH
AAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERKMMRKFMIDSVTYWAKEYNLDGF
HHHCCHHHCCCCEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
RFDLMGIHDYETMNEIRKAVNQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKGIAHF
EEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHEECCCCCHHHHHHHHHHC
NDNIRDGLKGSVFEEKENGFVNGKEHMEDRIKKGITAGIDYDTNSSTYQDPEQVLTYVEA
CCCHHCCCCCCCEECCCCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHHHC
HDNHTLWDKLELTNPSDSEEVRKQMHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNSYK
CCCCEEEEEEEECCCCCHHHHHHHHHHHHCEEEEEECCCCCEECCHHHHHHHCCCCCCCC
SPDSINQMDWLRRATFNNEVDYMKGLIELRKKYSAFRMTSAEQIKTHVSFIDAPKNTIAY
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEECCCCEEEE
TIEGNKNEYFTVAHNANRETVEITLPSKGPWKVLVDGKQAGSKSLYVVHDNKIKVPALSS
EEECCCCEEEEEEECCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEEECCEECCCCHHH
FVLKTEKPIK
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9453151; 10360571 [H]