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The map label for this gene is dadA [H]

Identifier: 222096230

GI number: 222096230

Start: 2526087

End: 2527202

Strand: Reverse

Name: dadA [H]

Synonym: BCQ_2570

Alternate gene names: 222096230

Gene position: 2527202-2526087 (Counterclockwise)

Preceding gene: 222096231

Following gene: 222096229

Centisome position: 48.47

GC content: 39.34

Gene sequence:

>1116_bases
ATGAAATCGTATATTGTAGTTGGAGCGGGAATTTTAGGTGCGTCTACTGCTTACCATCTTGCTAAGGCTGGCGCGAACGT
TACTATCGTGGATCGTCAACAAGTAGGTCAAGCAACAGATGCAGCAGCTGGTATTGTTTGCCCATGGCTTTCACAACGTC
GTAATAAAGCATGGTATAAAATCGTTAAAGGCGGTGCACGTTACTATTCTTCGTTAATTCAGCAATTAGAAGAAGACGGT
GAAACGGATACAGGTTATAACCGTGTAGGTGCAATTAGTTTACATACTGATGAGAAAAAACTAGATCAAATGGAAGAGCG
AGCTTATAAGCGCCGTGAAGATGCACCAGAGATTGGTGAAATTACGCGTTTATCAGTTGAAGAAACGAAAAAATTATTCC
CAGCATTATCAGAAGAATATAGTTCTGTTCATATTAGTGGTGCTGCACGAGTAAATGGAAGATTATTACGCAACGCATTA
ATAAGCGCTGCGAAAAAACATGGTGCAACTTTTATAAAAGGCAATGCAGTATTAGTCTGTGAAGGTAGTCAGATTACGGG
TGTTAAAGTAAACGATAAAACAATTCTAGCAGAAAAGGTTATTGTAACTGCAGGTGCATGGGCGAACGAAATCTTAAACC
CATTAGGAATTAATTTCTTAGTTACGTTTCAAAAAGGACAAATTGTTCACCTGCAAATGGAAAATACAGCAACAGAAAAT
ATGCCCGTTGTTATGCCGCCAAATGATCAATACATTTTAACATTTGATAATGGCCATGTCGTAATTGGTGCTACCCATGA
AAATGAAACTGGCTTCGATCATCGTGTAACGGCTGGTGGTTTAAATGAAGTATTCCATAAAGCATTAACAGTTGCACCTG
GCTTAGAAAATGCTACTATGCTTGAAACGAGAGTTGGATTCAGACCATTTACTCCAGGGTTCTTACCTGTAATTGGCCCA
CTTCCTAACTTTGAAGGTATTCTCGTTGCAAACGGATTAGGTGCCTCAGGTTTAACTGCAGGTCCATATTTAGGATCAGA
ACTTGCTAAGTTAGCGCTGGGACAACCAATTGAATTAGATTTAAATGATTATGACGTTGCAGGCGCAATTGAATAA

Upstream 100 bases:

>100_bases
CAATTAATTGCGATGAGTACATTGAAAAAAGTATTCCTTCCATTATACTAAGGGATGTTCTTATATTTATATAATTAGTA
AAAGAGAAAAGGTGTTTGAA

Downstream 100 bases:

>100_bases
CATAAGATATAGAAAAAATACGCTAAGCAAATTCGATAGGAAATAAGCAGTTTTTTTCTCTGTTAAGAGTAAGACTATTT
ATGAAAAATAAAATACTTTA

Product: d-amino acid dehydrogenase small subunit

Products: 2-oxo acid; NH3; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKSYIVVGAGILGASTAYHLAKAGANVTIVDRQQVGQATDAAAGIVCPWLSQRRNKAWYKIVKGGARYYSSLIQQLEEDG
ETDTGYNRVGAISLHTDEKKLDQMEERAYKRREDAPEIGEITRLSVEETKKLFPALSEEYSSVHISGAARVNGRLLRNAL
ISAAKKHGATFIKGNAVLVCEGSQITGVKVNDKTILAEKVIVTAGAWANEILNPLGINFLVTFQKGQIVHLQMENTATEN
MPVVMPPNDQYILTFDNGHVVIGATHENETGFDHRVTAGGLNEVFHKALTVAPGLENATMLETRVGFRPFTPGFLPVIGP
LPNFEGILVANGLGASGLTAGPYLGSELAKLALGQPIELDLNDYDVAGAIE

Sequences:

>Translated_371_residues
MKSYIVVGAGILGASTAYHLAKAGANVTIVDRQQVGQATDAAAGIVCPWLSQRRNKAWYKIVKGGARYYSSLIQQLEEDG
ETDTGYNRVGAISLHTDEKKLDQMEERAYKRREDAPEIGEITRLSVEETKKLFPALSEEYSSVHISGAARVNGRLLRNAL
ISAAKKHGATFIKGNAVLVCEGSQITGVKVNDKTILAEKVIVTAGAWANEILNPLGINFLVTFQKGQIVHLQMENTATEN
MPVVMPPNDQYILTFDNGHVVIGATHENETGFDHRVTAGGLNEVFHKALTVAPGLENATMLETRVGFRPFTPGFLPVIGP
LPNFEGILVANGLGASGLTAGPYLGSELAKLALGQPIELDLNDYDVAGAIE
>Mature_371_residues
MKSYIVVGAGILGASTAYHLAKAGANVTIVDRQQVGQATDAAAGIVCPWLSQRRNKAWYKIVKGGARYYSSLIQQLEEDG
ETDTGYNRVGAISLHTDEKKLDQMEERAYKRREDAPEIGEITRLSVEETKKLFPALSEEYSSVHISGAARVNGRLLRNAL
ISAAKKHGATFIKGNAVLVCEGSQITGVKVNDKTILAEKVIVTAGAWANEILNPLGINFLVTFQKGQIVHLQMENTATEN
MPVVMPPNDQYILTFDNGHVVIGATHENETGFDHRVTAGGLNEVFHKALTVAPGLENATMLETRVGFRPFTPGFLPVIGP
LPNFEGILVANGLGASGLTAGPYLGSELAKLALGQPIELDLNDYDVAGAIE

Specific function: Oxidative Deamination Of D-Amino Acids. [C]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI197927446, Length=386, Percent_Identity=24.6113989637306, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI21361378, Length=386, Percent_Identity=24.6113989637306, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI24797151, Length=401, Percent_Identity=24.1895261845387, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI20130091, Length=385, Percent_Identity=27.012987012987, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1

Molecular weight: Translated: 39893; Mature: 39893

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSYIVVGAGILGASTAYHLAKAGANVTIVDRQQVGQATDAAAGIVCPWLSQRRNKAWYK
CCCEEEEECCHHHCHHHHHHHHCCCCEEEEEHHHCCCHHHCCCCEECCHHHHHCCHHHHH
IVKGGARYYSSLIQQLEEDGETDTGYNRVGAISLHTDEKKLDQMEERAYKRREDAPEIGE
HHHCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCH
ITRLSVEETKKLFPALSEEYSSVHISGAARVNGRLLRNALISAAKKHGATFIKGNAVLVC
HEEECHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEE
EGSQITGVKVNDKTILAEKVIVTAGAWANEILNPLGINFLVTFQKGQIVHLQMENTATEN
CCCEEEEEEECCCEEEEEEEEEECCCCHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCC
MPVVMPPNDQYILTFDNGHVVIGATHENETGFDHRVTAGGLNEVFHKALTVAPGLENATM
CCEEECCCCCEEEEECCCEEEEEEECCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCEE
LETRVGFRPFTPGFLPVIGPLPNFEGILVANGLGASGLTAGPYLGSELAKLALGQPIELD
EEHCCCCCCCCCCCCHHCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEC
LNDYDVAGAIE
CCCCCCCCCCC
>Mature Secondary Structure
MKSYIVVGAGILGASTAYHLAKAGANVTIVDRQQVGQATDAAAGIVCPWLSQRRNKAWYK
CCCEEEEECCHHHCHHHHHHHHCCCCEEEEEHHHCCCHHHCCCCEECCHHHHHCCHHHHH
IVKGGARYYSSLIQQLEEDGETDTGYNRVGAISLHTDEKKLDQMEERAYKRREDAPEIGE
HHHCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCH
ITRLSVEETKKLFPALSEEYSSVHISGAARVNGRLLRNALISAAKKHGATFIKGNAVLVC
HEEECHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEE
EGSQITGVKVNDKTILAEKVIVTAGAWANEILNPLGINFLVTFQKGQIVHLQMENTATEN
CCCEEEEEEECCCEEEEEEEEEECCCCHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCC
MPVVMPPNDQYILTFDNGHVVIGATHENETGFDHRVTAGGLNEVFHKALTVAPGLENATM
CCEEECCCCCEEEEECCCEEEEEEECCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCEE
LETRVGFRPFTPGFLPVIGPLPNFEGILVANGLGASGLTAGPYLGSELAKLALGQPIELD
EEHCCCCCCCCCCCCHHCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEC
LNDYDVAGAIE
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-amino acid; H2O; acceptor

Specific reaction: a D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]