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The map label for this gene is cwlJ [H]

Identifier: 222096131

GI number: 222096131

Start: 2432849

End: 2433277

Strand: Reverse

Name: cwlJ [H]

Synonym: BCQ_2471

Alternate gene names: 222096131

Gene position: 2433277-2432849 (Counterclockwise)

Preceding gene: 222096135

Following gene: 222096129

Centisome position: 46.67

GC content: 43.82

Gene sequence:

>429_bases
ATGCCCCTTATTCCATATAACGAAAGCGATGTTGATTTACTAGCTCGTTTAATGCGTGCTGAAGCTGAAGGCGAAGGTCG
ACAAGGTGAACAACTTGTTGGCTGCGTTGTAGTAAATCGTGTATTTTGTGATTGTTTAGACTTTAAACAACTCCGTACCG
TACGTGACGCTGTGTATCAAAGCCCTGGTGGATTCGAAGCTGTGCAATACGGATATTTTTATCAACGGGCTCGTGAATCA
GAAAAAGAAATCGCACGAAAAGTTTTACAAGGTGAGTGGCGCTGGCCAGCAAGATGGGCTCTTTGGTATTTCCGTCCAGT
TGGAGCTTGTCCGCCTGAGTGGTATAATCAGCCGTTTGTAGGACAGTTTAAAAGTCATTGTTTTTATGAACCTAGTGGTG
ACGAATGTCCGAAATTGTATTCACGGTAG

Upstream 100 bases:

>100_bases
GCTCCTTTTAATAATTAATAAATTTACATATAGAAAAGCACTTAGCTTATTTTCATACATACACTCTTTACTATAACAGG
TAAAGAAAGGTGGTATCACT

Downstream 100 bases:

>100_bases
TAAAATTTGGAACGCAAGTACTTAGCTGAGTACTTGCGTTGCTTTTTTTAGAAATCTAATAATTCGATAAATTTTTCGAT
ACCTACAACATGTATTAATA

Product: cell wall hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 142; Mature: 141

Protein sequence:

>142_residues
MPLIPYNESDVDLLARLMRAEAEGEGRQGEQLVGCVVVNRVFCDCLDFKQLRTVRDAVYQSPGGFEAVQYGYFYQRARES
EKEIARKVLQGEWRWPARWALWYFRPVGACPPEWYNQPFVGQFKSHCFYEPSGDECPKLYSR

Sequences:

>Translated_142_residues
MPLIPYNESDVDLLARLMRAEAEGEGRQGEQLVGCVVVNRVFCDCLDFKQLRTVRDAVYQSPGGFEAVQYGYFYQRARES
EKEIARKVLQGEWRWPARWALWYFRPVGACPPEWYNQPFVGQFKSHCFYEPSGDECPKLYSR
>Mature_141_residues
PLIPYNESDVDLLARLMRAEAEGEGRQGEQLVGCVVVNRVFCDCLDFKQLRTVRDAVYQSPGGFEAVQYGYFYQRARESE
KEIARKVLQGEWRWPARWALWYFRPVGACPPEWYNQPFVGQFKSHCFYEPSGDECPKLYSR

Specific function: Probable spore cortex-lytic enzyme involved in the depolymerization of cortex peptidoglycan during germination [H]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CwlJ family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105 [H]

Pfam domain/function: PF07486 Hydrolase_2 [H]

EC number: 3.5.1.28

Molecular weight: Translated: 16630; Mature: 16499

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLIPYNESDVDLLARLMRAEAEGEGRQGEQLVGCVVVNRVFCDCLDFKQLRTVRDAVYQ
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHC
SPGGFEAVQYGYFYQRARESEKEIARKVLQGEWRWPARWALWYFRPVGACPPEWYNQPFV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHCCCCCC
GQFKSHCFYEPSGDECPKLYSR
HHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PLIPYNESDVDLLARLMRAEAEGEGRQGEQLVGCVVVNRVFCDCLDFKQLRTVRDAVYQ
CCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHC
SPGGFEAVQYGYFYQRARESEKEIARKVLQGEWRWPARWALWYFRPVGACPPEWYNQPFV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHCCCCCC
GQFKSHCFYEPSGDECPKLYSR
HHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704254; 9384377; 9515903; 11466292; 11807087; 12177332 [H]