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The map label for this gene is ydaP [H]

Identifier: 222095982

GI number: 222095982

Start: 2289980

End: 2291680

Strand: Reverse

Name: ydaP [H]

Synonym: BCQ_2322

Alternate gene names: 222095982

Gene position: 2291680-2289980 (Counterclockwise)

Preceding gene: 222095983

Following gene: 222095968

Centisome position: 43.95

GC content: 38.21

Gene sequence:

>1701_bases
ATGTTTGGAAAAACAGCCGGTGAAGCATTAGTTGATTTATTAATTGACTGGGGCGTAGAACACATATACGGTATGCCTGG
AGATTCCATCAATTCTATCATTGAAGCTTTAAGAAAAAAACAAGATAAAATTAAATTCATTCAAGTTCGCCACGAAGAAG
CTGGTGCATTAGCAGCGGCTGCCTATGCGAAATTAACAGGAAAACTTGGCGTTTGTATGGCAATTGCAGGACCTGGTGCT
ATTCATTTATTAAATGGTTTATATGATGCGAAACTCGATAAAGCCCCTGTTCTAGCAATTTCAGGTCAAGTAGAAACCGA
TTTACTCGGAACGGATTTTTTCCAAGAAGTAAATTTAGAAAGAATGTTTGATGATGTCGCGGTTTATAACCAGCGTATTA
TGTCAGCAGAGCAACTACCTGCCGTAGTGAATCAAGCAATTCGAATGGCTTATACGAAAAAAGGTGTATCCGTTCTTACA
ATTCCAGATGATGTCCCGAAATTCGAAGTAAAAAATGGTGCTCGTATTACAAATACTTCTTTTACTTTGCCTGAGTTGTT
TCCACAAAAAGAAGATTTAGATGCAGCAAAACTCGCAATAAATGCAGCGACAAAACCTGTTATTTTAGCTGGTAAAGGAG
CAAAACATGCGAGAGAATCTTTACTTGCATTTGCAGAACATATTGGTGCTCCAATCGTACTTACATTACCAGCTAAAGGC
GTTATTCCAGATGAACACCCTCTTTGTATTGGTGGATTAGGATTAATCGGAACGAAACCTTCTTATGAAGCAATGAAGCA
TGCAGATACTTTAATTATGGTTGGTACTTCTTATCCGTTCACCGGTTTCTTACCTGAAAAAGCTAAGACAATTCATATCG
ATACAGATCCTGCCCAAATTGGGAAACGTTATGCGACAGATATTGGTTTAGCTGGTGATGCTGAAAAAACTTTACGATGG
TTAATTGAAAATGTTGAAGAACACACAGACCATTCCTTCTTAGAACATTACCAAGACATGATGAAAAAGTGGGAAGAAAA
ATTACACGATCAAGAAGAAGACTCTTCCATTCCAATTAAACCACAACGCGTTATGCATGCTCTGCAGCAAGTAGCACATG
ATGACGCAATTCTATCAGTCGATGTCGGAAATGTAACAGTATGGACAGCAAGGCACTTCCGTATGACAAATCAACAATTT
ATCATTTCTAGCTGGCTCGCAACACTTGGTTGCGGTTTACCTGGTGCTCTTGCTGGAAAAATTGCATTCCCGGATAAACA
AGTATTTGCAGTTTGTGGTGACGGTGGTTTTGGAATGACAATGAACGACTTCGTAACAGCTGTTAAATATAAACTACCAA
TTGTTGTCGTCGTATTAAACAATAATAAAATTGCGATGATTAAATTCGAGCAAGAAGTCATGGGTAATATCGAATTCGAA
ACAGACTTAACGAACCCTGATTTTGCAAAATATGCAGAGGCGTGTGGAGGTATCGGATACCGCGTTGAACATATAGATGA
ATTACTCCCAGCTTTTGAAAACGCAGTAAAACAAAACAAACCATGTATTATCGATGTAGTTGTAGATGCTAACGAAGCAC
CAATGCCAGCAAAAATCACATTTGGTCAAGCTGCTGGTTATACGAAGCATATGATAAAAGAATTATTTGAAGAAGGTAAG
ATTGATTTACCACCACTATAA

Upstream 100 bases:

>100_bases
TTCGCTGGTCATCATTAAAGATAATAATATTATCATTTGTATATTTTTCCTTTTTCTCCAAAACATAATAGTATCTACCT
AATTATAAGGAGGAACAATT

Downstream 100 bases:

>100_bases
ACAGAAAAAGATAGCTGAAAACAAGTTGCTTTCAGCTATCTTTTTATTTTTGTATCTCTCTATAAAAAGAAAAGTCATTC
GTAGTTGGTTGTACTTGTTT

Product: pyruvate oxidase

Products: acetyl phosphate; CO2; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MFGKTAGEALVDLLIDWGVEHIYGMPGDSINSIIEALRKKQDKIKFIQVRHEEAGALAAAAYAKLTGKLGVCMAIAGPGA
IHLLNGLYDAKLDKAPVLAISGQVETDLLGTDFFQEVNLERMFDDVAVYNQRIMSAEQLPAVVNQAIRMAYTKKGVSVLT
IPDDVPKFEVKNGARITNTSFTLPELFPQKEDLDAAKLAINAATKPVILAGKGAKHARESLLAFAEHIGAPIVLTLPAKG
VIPDEHPLCIGGLGLIGTKPSYEAMKHADTLIMVGTSYPFTGFLPEKAKTIHIDTDPAQIGKRYATDIGLAGDAEKTLRW
LIENVEEHTDHSFLEHYQDMMKKWEEKLHDQEEDSSIPIKPQRVMHALQQVAHDDAILSVDVGNVTVWTARHFRMTNQQF
IISSWLATLGCGLPGALAGKIAFPDKQVFAVCGDGGFGMTMNDFVTAVKYKLPIVVVVLNNNKIAMIKFEQEVMGNIEFE
TDLTNPDFAKYAEACGGIGYRVEHIDELLPAFENAVKQNKPCIIDVVVDANEAPMPAKITFGQAAGYTKHMIKELFEEGK
IDLPPL

Sequences:

>Translated_566_residues
MFGKTAGEALVDLLIDWGVEHIYGMPGDSINSIIEALRKKQDKIKFIQVRHEEAGALAAAAYAKLTGKLGVCMAIAGPGA
IHLLNGLYDAKLDKAPVLAISGQVETDLLGTDFFQEVNLERMFDDVAVYNQRIMSAEQLPAVVNQAIRMAYTKKGVSVLT
IPDDVPKFEVKNGARITNTSFTLPELFPQKEDLDAAKLAINAATKPVILAGKGAKHARESLLAFAEHIGAPIVLTLPAKG
VIPDEHPLCIGGLGLIGTKPSYEAMKHADTLIMVGTSYPFTGFLPEKAKTIHIDTDPAQIGKRYATDIGLAGDAEKTLRW
LIENVEEHTDHSFLEHYQDMMKKWEEKLHDQEEDSSIPIKPQRVMHALQQVAHDDAILSVDVGNVTVWTARHFRMTNQQF
IISSWLATLGCGLPGALAGKIAFPDKQVFAVCGDGGFGMTMNDFVTAVKYKLPIVVVVLNNNKIAMIKFEQEVMGNIEFE
TDLTNPDFAKYAEACGGIGYRVEHIDELLPAFENAVKQNKPCIIDVVVDANEAPMPAKITFGQAAGYTKHMIKELFEEGK
IDLPPL
>Mature_566_residues
MFGKTAGEALVDLLIDWGVEHIYGMPGDSINSIIEALRKKQDKIKFIQVRHEEAGALAAAAYAKLTGKLGVCMAIAGPGA
IHLLNGLYDAKLDKAPVLAISGQVETDLLGTDFFQEVNLERMFDDVAVYNQRIMSAEQLPAVVNQAIRMAYTKKGVSVLT
IPDDVPKFEVKNGARITNTSFTLPELFPQKEDLDAAKLAINAATKPVILAGKGAKHARESLLAFAEHIGAPIVLTLPAKG
VIPDEHPLCIGGLGLIGTKPSYEAMKHADTLIMVGTSYPFTGFLPEKAKTIHIDTDPAQIGKRYATDIGLAGDAEKTLRW
LIENVEEHTDHSFLEHYQDMMKKWEEKLHDQEEDSSIPIKPQRVMHALQQVAHDDAILSVDVGNVTVWTARHFRMTNQQF
IISSWLATLGCGLPGALAGKIAFPDKQVFAVCGDGGFGMTMNDFVTAVKYKLPIVVVVLNNNKIAMIKFEQEVMGNIEFE
TDLTNPDFAKYAEACGGIGYRVEHIDELLPAFENAVKQNKPCIIDVVVDANEAPMPAKITFGQAAGYTKHMIKELFEEGK
IDLPPL

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=552, Percent_Identity=26.8115942028986, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI21361361, Length=584, Percent_Identity=23.1164383561644, Blast_Score=125, Evalue=1e-28,
Organism=Escherichia coli, GI1787096, Length=555, Percent_Identity=35.1351351351351, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI87081685, Length=570, Percent_Identity=30.8771929824561, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1790104, Length=548, Percent_Identity=29.1970802919708, Blast_Score=237, Evalue=2e-63,
Organism=Escherichia coli, GI1786717, Length=555, Percent_Identity=26.3063063063063, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI1788716, Length=545, Percent_Identity=24.2201834862385, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17531299, Length=548, Percent_Identity=24.6350364963504, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17531301, Length=548, Percent_Identity=24.6350364963504, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17542570, Length=557, Percent_Identity=24.5960502692998, Blast_Score=128, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=567, Percent_Identity=29.1005291005291, Blast_Score=225, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6320816, Length=561, Percent_Identity=24.5989304812834, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323163, Length=497, Percent_Identity=23.3400402414487, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI19922626, Length=569, Percent_Identity=25.8347978910369, Blast_Score=170, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.3.3

Molecular weight: Translated: 61898; Mature: 61898

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGKTAGEALVDLLIDWGVEHIYGMPGDSINSIIEALRKKQDKIKFIQVRHEEAGALAAA
CCCCHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEECHHHCHHHHH
AYAKLTGKLGVCMAIAGPGAIHLLNGLYDAKLDKAPVLAISGQVETDLLGTDFFQEVNLE
HHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCHHCHHHHHHCCHH
RMFDDVAVYNQRIMSAEQLPAVVNQAIRMAYTKKGVSVLTIPDDVPKFEVKNGARITNTS
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCEEECCC
FTLPELFPQKEDLDAAKLAINAATKPVILAGKGAKHARESLLAFAEHIGAPIVLTLPAKG
CCCHHHCCCCCCCCHHHEEEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCC
VIPDEHPLCIGGLGLIGTKPSYEAMKHADTLIMVGTSYPFTGFLPEKAKTIHIDTDPAQI
CCCCCCCEEEECCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHH
GKRYATDIGLAGDAEKTLRWLIENVEEHTDHSFLEHYQDMMKKWEEKLHDQEEDSSIPIK
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PQRVMHALQQVAHDDAILSVDVGNVTVWTARHFRMTNQQFIISSWLATLGCGLPGALAGK
HHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEEEECCCHHHHHHHHHHHCCCCCCHHCCC
IAFPDKQVFAVCGDGGFGMTMNDFVTAVKYKLPIVVVVLNNNKIAMIKFEQEVMGNIEFE
EECCCCEEEEEECCCCCCCCHHHHHHHHEEECCEEEEEECCCEEEEEEECHHHHCCEEEE
TDLTNPDFAKYAEACGGIGYRVEHIDELLPAFENAVKQNKPCIIDVVVDANEAPMPAKIT
ECCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEE
FGQAAGYTKHMIKELFEEGKIDLPPL
CCCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MFGKTAGEALVDLLIDWGVEHIYGMPGDSINSIIEALRKKQDKIKFIQVRHEEAGALAAA
CCCCHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEECHHHCHHHHH
AYAKLTGKLGVCMAIAGPGAIHLLNGLYDAKLDKAPVLAISGQVETDLLGTDFFQEVNLE
HHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCHHCHHHHHHCCHH
RMFDDVAVYNQRIMSAEQLPAVVNQAIRMAYTKKGVSVLTIPDDVPKFEVKNGARITNTS
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCEEECCC
FTLPELFPQKEDLDAAKLAINAATKPVILAGKGAKHARESLLAFAEHIGAPIVLTLPAKG
CCCHHHCCCCCCCCHHHEEEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCC
VIPDEHPLCIGGLGLIGTKPSYEAMKHADTLIMVGTSYPFTGFLPEKAKTIHIDTDPAQI
CCCCCCCEEEECCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHH
GKRYATDIGLAGDAEKTLRWLIENVEEHTDHSFLEHYQDMMKKWEEKLHDQEEDSSIPIK
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PQRVMHALQQVAHDDAILSVDVGNVTVWTARHFRMTNQQFIISSWLATLGCGLPGALAGK
HHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEEEECCCHHHHHHHHHHHCCCCCCHHCCC
IAFPDKQVFAVCGDGGFGMTMNDFVTAVKYKLPIVVVVLNNNKIAMIKFEQEVMGNIEFE
EECCCCEEEEEECCCCCCCCHHHHHHHHEEECCEEEEEECCCEEEEEEECHHHHCCEEEE
TDLTNPDFAKYAEACGGIGYRVEHIDELLPAFENAVKQNKPCIIDVVVDANEAPMPAKIT
ECCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEE
FGQAAGYTKHMIKELFEEGKIDLPPL
CCCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; phosphate; O2; H2O

Specific reaction: pyruvate + phosphate + O2 + H2O = acetyl phosphate + CO2 + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]