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The map label for this gene is nirB [H]

Identifier: 222095781

GI number: 222095781

Start: 2090127

End: 2092532

Strand: Reverse

Name: nirB [H]

Synonym: BCQ_2121

Alternate gene names: 222095781

Gene position: 2092532-2090127 (Counterclockwise)

Preceding gene: 222095782

Following gene: 222095780

Centisome position: 40.13

GC content: 37.78

Gene sequence:

>2406_bases
ATGAAAAAACGTTTAGTTATGATCGGAAATGGTATGGCTGGCATACGTTGTATGGAAGAAATATTAAAACATGATTGTGA
TTCATATGAGATTACTATTTTTGGAGATGAACCACACCCAAACTATAATCGCATTATGCTCTCTCATGTTTTACAAGGTA
AAACAAATATACAAGATATCATTATGAATGAATACAGTTGGTATGAAGAAAACGAAATAACTTTGTATACAAATGAAAGA
GTTCAAAGTATTGACCGAGAAGAAAAAGTTATTATTACAGAAAAGAATCGTACTCTTACATATGACAAGCTTATTATTGC
AACAGGTTCTAGTGCTTTTATTTTACCGGTTGAAGGTTCCACTCTTCCTGGTGTAACAGGTTTTCGAACTATTGAAGATA
CACAGTTTATGATGGATACTGCTAAAGAAAAGAAAAAGGCCGTTGTCATTGGTGGTGGATTACTTGGTTTAGAAGCAGCA
AGAGGTCTTATTGACTTAGGAATGGACGTACATGTTGTTCATTTAATGCCTAGCTTAATGGAGCAACAACTAGATACGAA
AGCAGCTTCTCTACTGCGTGAAGATTTAGAAGCACAAGGCATGAAGTTTTTAATGGAAAAGAAAACTGTAAAAATTCTTG
GTACAAACCATGTTGAGGGCATTCAATTTGAAGATGGTGAAGTTGTAGATTGTGATTTAATCGTAATGGCTGTCGGTATC
CGACCAAATACGCAAATAGCAAGAGATGCTGGTTTAATTGTCAATCGCGGCATTGTAGTCAATGACTATATGCTAACAAA
TGATGAGTCCATTTATGCAGTTGGCGAATGTGCAGAACATGACGGTATCGCATATGGACTTGTTGCCCCTCTTTATGAAC
AAGGTGCAATATTAGCAAAACATATAACAAATTTACAAACTGATGGATATTCGGGCAGTATCGTCGGTACGCAATTAAAA
GTTGCTGGTTGTGATTTATTCTCTGCAGGCCAAATTTATGAAGATGATCAAACGAAAGCAATATCCATCTTTAATGAATG
CAAACGTTCCTATAAAAAAGTATTAATTCGTGACAATAAAGTCGTTGGTATCGTTTTATATGGTGACACAGCTGATGGTA
CACGCCTCTTTAGCATGTTAAAGAAAGAAGAAGATATACAAGAATATACACCAGCGTCCATTCTTCACAAAGCTGGTGAA
GAAAGTGAACTTGATGTTGCTACAATGAGTGCGGATGACACGATTTGTGGATGTAATGGTGTTACGAAAGGAACAATTGT
TCATGCCATTTTAGAACAAGAGTTAACGACTTTTGAAGAAGTTAAAGGCTGTACGAAAGCCGCAGGGTCTTGTGGTAAAT
GTCGTCCGCTTGTAGAACAAGTTTTATCTCATACACTCGGAGATGCTTTTGATGCCTCAGCCCAGTCTGCTGGTATGTGC
GGATGTACACCTTTATCACGTGATGAAGTCGTAGCGGCAATTCATGAAAAAGGCTTAAAATCACCAAAAGAAGTACGAAA
TGTTCTTGGTTTTGTACATGAAGATGGCTGTTCAAAATGTCGTCCTGCTTTGAACTACTATTTACGTATGGCGATTCCAG
AAGAATATGAAGATGATAAAGCCTCCCGTTTCGTTAATGAAAGAATGAATGGTAACATCCAGCACGATGGTACATTCTCT
GTTATTCCACGTATGTACGGAGGCGTTACAACAGCAGATGATTTAATGAAAATTGCTGAAGTTGCGAAAAAGTATGATGT
TCCACTTGTGAAAATTACCGGTGCAAGTCGAATTGGCTTATACGGTGTTAAGAAACAAGATTTACCTAACGTATGGGCTG
ACTTAAATATGACTTCTGGATATGCGTATTCAAAATCTCTTCGTAATGTAAAATCATGTGTTGGCTCTCGTTTCTGCCGT
TTCGGTACGAAAGATTCATTAGGACTTGGTATGCTCCTTGAACAATCATTAGAAATGGTAGATACACCTCATAAAATGAA
GATGGGTGTAACGGGCTGTCCGCGTAACTGTGCGGAAGTACTTACGAAAGATTTTGGCGTTGTTTGTGTCGAAAATGGAT
ACCAACTTTATATTGGTGGAAATGGTGGTACAGAAGTACGTGAAGCTGATTTTGTAATGATTGTCCCTACTGAAGATGAT
GTTCTTCACATCGCTACAGCTTACATGCAATATTATCGTGAAACTGGTATTTACGGAGAGCGTACTGCCTACTGGACAGA
ACGATTAGGCTTCGATCACATAAAAGAAATACTGCAAGATGCAAATATGGTCACTAAGTTAAATGAACGTTTCCAAACGG
CCCGTGGCACATATAAAGAAGCTTGGGGACAAGCACTAGAAACGAAATCATTAAAAGCGATGTATGAAGTAGAAACTGTG
AAATAA

Upstream 100 bases:

>100_bases
TATAAAAATGTGATAAATATCACATCTCCCCCTTTCTTGATTTTCTATAATAAAAATATAATCCTTTGTTCAACATTTCA
CATAAAAAGGGGAAACGAAA

Downstream 100 bases:

>100_bases
GGAGCTGTGATCTATATGATACAAACGAAAGATAAATTAAAAGTTATGCGTGCAGAAGATCTTCCTATTCAAATCGGTAA
AGAGGTCCAAATGAAAGGTA

Product: nitrite reductase [nad(p)h], large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 801; Mature: 801

Protein sequence:

>801_residues
MKKRLVMIGNGMAGIRCMEEILKHDCDSYEITIFGDEPHPNYNRIMLSHVLQGKTNIQDIIMNEYSWYEENEITLYTNER
VQSIDREEKVIITEKNRTLTYDKLIIATGSSAFILPVEGSTLPGVTGFRTIEDTQFMMDTAKEKKKAVVIGGGLLGLEAA
RGLIDLGMDVHVVHLMPSLMEQQLDTKAASLLREDLEAQGMKFLMEKKTVKILGTNHVEGIQFEDGEVVDCDLIVMAVGI
RPNTQIARDAGLIVNRGIVVNDYMLTNDESIYAVGECAEHDGIAYGLVAPLYEQGAILAKHITNLQTDGYSGSIVGTQLK
VAGCDLFSAGQIYEDDQTKAISIFNECKRSYKKVLIRDNKVVGIVLYGDTADGTRLFSMLKKEEDIQEYTPASILHKAGE
ESELDVATMSADDTICGCNGVTKGTIVHAILEQELTTFEEVKGCTKAAGSCGKCRPLVEQVLSHTLGDAFDASAQSAGMC
GCTPLSRDEVVAAIHEKGLKSPKEVRNVLGFVHEDGCSKCRPALNYYLRMAIPEEYEDDKASRFVNERMNGNIQHDGTFS
VIPRMYGGVTTADDLMKIAEVAKKYDVPLVKITGASRIGLYGVKKQDLPNVWADLNMTSGYAYSKSLRNVKSCVGSRFCR
FGTKDSLGLGMLLEQSLEMVDTPHKMKMGVTGCPRNCAEVLTKDFGVVCVENGYQLYIGGNGGTEVREADFVMIVPTEDD
VLHIATAYMQYYRETGIYGERTAYWTERLGFDHIKEILQDANMVTKLNERFQTARGTYKEAWGQALETKSLKAMYEVETV
K

Sequences:

>Translated_801_residues
MKKRLVMIGNGMAGIRCMEEILKHDCDSYEITIFGDEPHPNYNRIMLSHVLQGKTNIQDIIMNEYSWYEENEITLYTNER
VQSIDREEKVIITEKNRTLTYDKLIIATGSSAFILPVEGSTLPGVTGFRTIEDTQFMMDTAKEKKKAVVIGGGLLGLEAA
RGLIDLGMDVHVVHLMPSLMEQQLDTKAASLLREDLEAQGMKFLMEKKTVKILGTNHVEGIQFEDGEVVDCDLIVMAVGI
RPNTQIARDAGLIVNRGIVVNDYMLTNDESIYAVGECAEHDGIAYGLVAPLYEQGAILAKHITNLQTDGYSGSIVGTQLK
VAGCDLFSAGQIYEDDQTKAISIFNECKRSYKKVLIRDNKVVGIVLYGDTADGTRLFSMLKKEEDIQEYTPASILHKAGE
ESELDVATMSADDTICGCNGVTKGTIVHAILEQELTTFEEVKGCTKAAGSCGKCRPLVEQVLSHTLGDAFDASAQSAGMC
GCTPLSRDEVVAAIHEKGLKSPKEVRNVLGFVHEDGCSKCRPALNYYLRMAIPEEYEDDKASRFVNERMNGNIQHDGTFS
VIPRMYGGVTTADDLMKIAEVAKKYDVPLVKITGASRIGLYGVKKQDLPNVWADLNMTSGYAYSKSLRNVKSCVGSRFCR
FGTKDSLGLGMLLEQSLEMVDTPHKMKMGVTGCPRNCAEVLTKDFGVVCVENGYQLYIGGNGGTEVREADFVMIVPTEDD
VLHIATAYMQYYRETGIYGERTAYWTERLGFDHIKEILQDANMVTKLNERFQTARGTYKEAWGQALETKSLKAMYEVETV
K
>Mature_801_residues
MKKRLVMIGNGMAGIRCMEEILKHDCDSYEITIFGDEPHPNYNRIMLSHVLQGKTNIQDIIMNEYSWYEENEITLYTNER
VQSIDREEKVIITEKNRTLTYDKLIIATGSSAFILPVEGSTLPGVTGFRTIEDTQFMMDTAKEKKKAVVIGGGLLGLEAA
RGLIDLGMDVHVVHLMPSLMEQQLDTKAASLLREDLEAQGMKFLMEKKTVKILGTNHVEGIQFEDGEVVDCDLIVMAVGI
RPNTQIARDAGLIVNRGIVVNDYMLTNDESIYAVGECAEHDGIAYGLVAPLYEQGAILAKHITNLQTDGYSGSIVGTQLK
VAGCDLFSAGQIYEDDQTKAISIFNECKRSYKKVLIRDNKVVGIVLYGDTADGTRLFSMLKKEEDIQEYTPASILHKAGE
ESELDVATMSADDTICGCNGVTKGTIVHAILEQELTTFEEVKGCTKAAGSCGKCRPLVEQVLSHTLGDAFDASAQSAGMC
GCTPLSRDEVVAAIHEKGLKSPKEVRNVLGFVHEDGCSKCRPALNYYLRMAIPEEYEDDKASRFVNERMNGNIQHDGTFS
VIPRMYGGVTTADDLMKIAEVAKKYDVPLVKITGASRIGLYGVKKQDLPNVWADLNMTSGYAYSKSLRNVKSCVGSRFCR
FGTKDSLGLGMLLEQSLEMVDTPHKMKMGVTGCPRNCAEVLTKDFGVVCVENGYQLYIGGNGGTEVREADFVMIVPTEDD
VLHIATAYMQYYRETGIYGERTAYWTERLGFDHIKEILQDANMVTKLNERFQTARGTYKEAWGQALETKSLKAMYEVETV
K

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=276, Percent_Identity=27.1739130434783, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI21389617, Length=276, Percent_Identity=27.1739130434783, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI226437568, Length=276, Percent_Identity=27.1739130434783, Blast_Score=98, Evalue=3e-20,
Organism=Homo sapiens, GI22202629, Length=266, Percent_Identity=26.6917293233083, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI4757732, Length=266, Percent_Identity=26.6917293233083, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI1789765, Length=816, Percent_Identity=35.171568627451, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1789065, Length=309, Percent_Identity=29.7734627831715, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788892, Length=280, Percent_Identity=24.6428571428571, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17559934, Length=405, Percent_Identity=22.962962962963, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI32565766, Length=210, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI281359715, Length=281, Percent_Identity=24.1992882562278, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281359713, Length=281, Percent_Identity=24.1992882562278, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24639250, Length=281, Percent_Identity=24.1992882562278, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI18543267, Length=281, Percent_Identity=24.1992882562278, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24639252, Length=281, Percent_Identity=24.1992882562278, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24639257, Length=281, Percent_Identity=24.1992882562278, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585130, Length=347, Percent_Identity=22.478386167147, Blast_Score=91, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 88665; Mature: 88665

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRLVMIGNGMAGIRCMEEILKHDCDSYEITIFGDEPHPNYNRIMLSHVLQGKTNIQDI
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHH
IMNEYSWYEENEITLYTNERVQSIDREEKVIITEKNRTLTYDKLIIATGSSAFILPVEGS
HHCCCCCCCCCCEEEEECHHHHHCCCCCEEEEEECCCEEEEEEEEEEECCCEEEEEECCC
TLPGVTGFRTIEDTQFMMDTAKEKKKAVVIGGGLLGLEAARGLIDLGMDVHVVHLMPSLM
CCCCCCCCEEECHHHHHHHHHHCCCCEEEEECCHHHHHHHCHHHHCCCCEEHHHHHHHHH
EQQLDTKAASLLREDLEAQGMKFLMEKKTVKILGTNHVEGIQFEDGEVVDCDLIVMAVGI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCCEEEEEEEEEEECC
RPNTQIARDAGLIVNRGIVVNDYMLTNDESIYAVGECAEHDGIAYGLVAPLYEQGAILAK
CCCCCHHHCCCEEEECCEEEEEEEEECCCCEEEEECCCCCCCEEEHHHHHHHHCCCHHHH
HITNLQTDGYSGSIVGTQLKVAGCDLFSAGQIYEDDQTKAISIFNECKRSYKKVLIRDNK
HHHHCCCCCCCCCEEEEEEEEECCCEECCCCEECCCCHHHHHHHHHHHHHHHHEEEECCC
VVGIVLYGDTADGTRLFSMLKKEEDIQEYTPASILHKAGEESELDVATMSADDTICGCNG
EEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEECCCCCEECCCC
VTKGTIVHAILEQELTTFEEVKGCTKAAGSCGKCRPLVEQVLSHTLGDAFDASAQSAGMC
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCCC
GCTPLSRDEVVAAIHEKGLKSPKEVRNVLGFVHEDGCSKCRPALNYYLRMAIPEEYEDDK
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCCHH
ASRFVNERMNGNIQHDGTFSVIPRMYGGVTTADDLMKIAEVAKKYDVPLVKITGASRIGL
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEECE
YGVKKQDLPNVWADLNMTSGYAYSKSLRNVKSCVGSRFCRFGTKDSLGLGMLLEQSLEMV
ECCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DTPHKMKMGVTGCPRNCAEVLTKDFGVVCVENGYQLYIGGNGGTEVREADFVMIVPTEDD
CCCCHHCCCCCCCCHHHHHHHHHCCCEEEEECCEEEEEECCCCCCCCCCCEEEEEECCCC
VLHIATAYMQYYRETGIYGERTAYWTERLGFDHIKEILQDANMVTKLNERFQTARGTYKE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
AWGQALETKSLKAMYEVETVK
HHHHHHHHHHHHHHEEEEECC
>Mature Secondary Structure
MKKRLVMIGNGMAGIRCMEEILKHDCDSYEITIFGDEPHPNYNRIMLSHVLQGKTNIQDI
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHH
IMNEYSWYEENEITLYTNERVQSIDREEKVIITEKNRTLTYDKLIIATGSSAFILPVEGS
HHCCCCCCCCCCEEEEECHHHHHCCCCCEEEEEECCCEEEEEEEEEEECCCEEEEEECCC
TLPGVTGFRTIEDTQFMMDTAKEKKKAVVIGGGLLGLEAARGLIDLGMDVHVVHLMPSLM
CCCCCCCCEEECHHHHHHHHHHCCCCEEEEECCHHHHHHHCHHHHCCCCEEHHHHHHHHH
EQQLDTKAASLLREDLEAQGMKFLMEKKTVKILGTNHVEGIQFEDGEVVDCDLIVMAVGI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCCEEEEEEEEEEECC
RPNTQIARDAGLIVNRGIVVNDYMLTNDESIYAVGECAEHDGIAYGLVAPLYEQGAILAK
CCCCCHHHCCCEEEECCEEEEEEEEECCCCEEEEECCCCCCCEEEHHHHHHHHCCCHHHH
HITNLQTDGYSGSIVGTQLKVAGCDLFSAGQIYEDDQTKAISIFNECKRSYKKVLIRDNK
HHHHCCCCCCCCCEEEEEEEEECCCEECCCCEECCCCHHHHHHHHHHHHHHHHEEEECCC
VVGIVLYGDTADGTRLFSMLKKEEDIQEYTPASILHKAGEESELDVATMSADDTICGCNG
EEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEECCCCCEECCCC
VTKGTIVHAILEQELTTFEEVKGCTKAAGSCGKCRPLVEQVLSHTLGDAFDASAQSAGMC
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCCC
GCTPLSRDEVVAAIHEKGLKSPKEVRNVLGFVHEDGCSKCRPALNYYLRMAIPEEYEDDK
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCCHH
ASRFVNERMNGNIQHDGTFSVIPRMYGGVTTADDLMKIAEVAKKYDVPLVKITGASRIGL
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEECE
YGVKKQDLPNVWADLNMTSGYAYSKSLRNVKSCVGSRFCRFGTKDSLGLGMLLEQSLEMV
ECCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DTPHKMKMGVTGCPRNCAEVLTKDFGVVCVENGYQLYIGGNGGTEVREADFVMIVPTEDD
CCCCHHCCCCCCCCHHHHHHHHHCCCEEEEECCEEEEEECCCCCCCCCCCEEEEEECCCC
VLHIATAYMQYYRETGIYGERTAYWTERLGFDHIKEILQDANMVTKLNERFQTARGTYKE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
AWGQALETKSLKAMYEVETVK
HHHHHHHHHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]