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The map label for this gene is narK [H]

Identifier: 222095772

GI number: 222095772

Start: 2083071

End: 2084240

Strand: Direct

Name: narK [H]

Synonym: BCQ_2112

Alternate gene names: 222095772

Gene position: 2083071-2084240 (Clockwise)

Preceding gene: 222095771

Following gene: 222095773

Centisome position: 39.95

GC content: 34.19

Gene sequence:

>1170_bases
ATGAAAAGTCCTAATTTTCAATTAAGTTTACAAACTTCTAATCTAATTATCGGTTTTATGGTGTGGGTTATTTTATCATC
ATTGATGCCCTATATTAAAGTGGATATTCCATTAACTGCGGGACAAATTTCTATGGTAACAGCAGTACCCGTTATTTTAG
GTTCTGTTCTTCGCATTCCAATTGGTTATTGGACCAATCGTTTCGGAGCAAGAAAATTATTCTTTATTAGTTTTATTCTA
TTATTATTTCCTGTTTTTTATATTAGTGTTGCCAATTCTATGATGGATTTAATTATTGGTGGATTATTTGTAGGAATCGG
TGGTGCTGTATTCTCTGTAGGAGTAACTTCTTTACCAAAATACTTTCCAAAAGAGAGTCACGGTTTTGTAAATGGTATTT
ACGGTGTCGGTAACGCTGGAACAGCAATTACTTCATTTTTAGCGCCTGTTATCGCAACTTCAGTTGGCTGGAGAACGACA
GTACAATGTTATTTAATTTTACTTGCAGCGTTTGCACTTATGAACTTTTTATTAGGTGATCGTAAAGAGAAAAAAGTGAA
TACACCACTAATGGAACAAATAAAAGGTGTATATAAAAATGAGAAACTTTGGTTTTTATGTATCTTTTACTTTTTAACTT
TCGGATCATTTGTTGCATTTACAGTATATTTACCAAACTTTTTAGTATCTCATTTCGGATTAGAGAAAGTAGATGCAGGT
ATACGTACAGCCGGATTCATCGTACTCGCAACAATTATGCGTCCGATTGGTGGTTGGCTCGGTGATAAGTTTAATCCATT
TAAAATATTAATCTTCGTATTTATCGGTTTAACACTTTCAGGTATTATTTTATCATTTATGCCTAGCATGAACGTATATA
CATTTGGTTGCCTACTTGTCGCATTTTGCGCAGGTATCGGTAATGGTACAATCTTCAAACTCGTTCCAATGTATTTCTCT
GAACAAGCTGGTATTGTAAACGGACTCGTTTCAGCTTTAGGCGGACTAGGAGGATTCTTCCCGCCTCTAATTTTAACATT
ACTATTCCAACTAACAGGTCATTATGCAATAGGATTTATGGCGTTATCAGAAGTCGCACTTGCTTGTTTAATTATTACAG
TCTGGATGTATAGTCAGGAGAAACTCTTAGTGATGTTAAAGAATCATTAA

Upstream 100 bases:

>100_bases
CGCCAGTAAGTGGTGGTTGATTCTTTTCAGCCTAATCATGTAAGAACGTGATTAGGCTGGAGGGAATGAATATAACTACA
TATACATAGGAGGGAACAGG

Downstream 100 bases:

>100_bases
TAAGGAAAAAGAACCGTCCCAAAGTATTTTGGGATGGTTCTTTTAGTAATAGGCTTCAACATGTTTTCTATTGATATCTT
AACGAACGTTTGTTTCCTGA

Product: nitrite extrusion protein

Products: NA

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MKSPNFQLSLQTSNLIIGFMVWVILSSLMPYIKVDIPLTAGQISMVTAVPVILGSVLRIPIGYWTNRFGARKLFFISFIL
LLFPVFYISVANSMMDLIIGGLFVGIGGAVFSVGVTSLPKYFPKESHGFVNGIYGVGNAGTAITSFLAPVIATSVGWRTT
VQCYLILLAAFALMNFLLGDRKEKKVNTPLMEQIKGVYKNEKLWFLCIFYFLTFGSFVAFTVYLPNFLVSHFGLEKVDAG
IRTAGFIVLATIMRPIGGWLGDKFNPFKILIFVFIGLTLSGIILSFMPSMNVYTFGCLLVAFCAGIGNGTIFKLVPMYFS
EQAGIVNGLVSALGGLGGFFPPLILTLLFQLTGHYAIGFMALSEVALACLIITVWMYSQEKLLVMLKNH

Sequences:

>Translated_389_residues
MKSPNFQLSLQTSNLIIGFMVWVILSSLMPYIKVDIPLTAGQISMVTAVPVILGSVLRIPIGYWTNRFGARKLFFISFIL
LLFPVFYISVANSMMDLIIGGLFVGIGGAVFSVGVTSLPKYFPKESHGFVNGIYGVGNAGTAITSFLAPVIATSVGWRTT
VQCYLILLAAFALMNFLLGDRKEKKVNTPLMEQIKGVYKNEKLWFLCIFYFLTFGSFVAFTVYLPNFLVSHFGLEKVDAG
IRTAGFIVLATIMRPIGGWLGDKFNPFKILIFVFIGLTLSGIILSFMPSMNVYTFGCLLVAFCAGIGNGTIFKLVPMYFS
EQAGIVNGLVSALGGLGGFFPPLILTLLFQLTGHYAIGFMALSEVALACLIITVWMYSQEKLLVMLKNH
>Mature_389_residues
MKSPNFQLSLQTSNLIIGFMVWVILSSLMPYIKVDIPLTAGQISMVTAVPVILGSVLRIPIGYWTNRFGARKLFFISFIL
LLFPVFYISVANSMMDLIIGGLFVGIGGAVFSVGVTSLPKYFPKESHGFVNGIYGVGNAGTAITSFLAPVIATSVGWRTT
VQCYLILLAAFALMNFLLGDRKEKKVNTPLMEQIKGVYKNEKLWFLCIFYFLTFGSFVAFTVYLPNFLVSHFGLEKVDAG
IRTAGFIVLATIMRPIGGWLGDKFNPFKILIFVFIGLTLSGIILSFMPSMNVYTFGCLLVAFCAGIGNGTIFKLVPMYFS
EQAGIVNGLVSALGGLGGFFPPLILTLLFQLTGHYAIGFMALSEVALACLIITVWMYSQEKLLVMLKNH

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=425, Percent_Identity=24.9411764705882, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787743, Length=441, Percent_Identity=21.5419501133787, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42646; Mature: 42646

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSPNFQLSLQTSNLIIGFMVWVILSSLMPYIKVDIPLTAGQISMVTAVPVILGSVLRIP
CCCCCEEEEEECCHHHHHHHHHHHHHHCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHHH
IGYWTNRFGARKLFFISFILLLFPVFYISVANSMMDLIIGGLFVGIGGAVFSVGVTSLPK
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFPKESHGFVNGIYGVGNAGTAITSFLAPVIATSVGWRTTVQCYLILLAAFALMNFLLGD
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
RKEKKVNTPLMEQIKGVYKNEKLWFLCIFYFLTFGSFVAFTVYLPNFLVSHFGLEKVDAG
CCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
IRTAGFIVLATIMRPIGGWLGDKFNPFKILIFVFIGLTLSGIILSFMPSMNVYTFGCLLV
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
AFCAGIGNGTIFKLVPMYFSEQAGIVNGLVSALGGLGGFFPPLILTLLFQLTGHYAIGFM
HHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ALSEVALACLIITVWMYSQEKLLVMLKNH
HHHHHHHHHHHHHHHHCCCCCEEEEEECC
>Mature Secondary Structure
MKSPNFQLSLQTSNLIIGFMVWVILSSLMPYIKVDIPLTAGQISMVTAVPVILGSVLRIP
CCCCCEEEEEECCHHHHHHHHHHHHHHCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHHH
IGYWTNRFGARKLFFISFILLLFPVFYISVANSMMDLIIGGLFVGIGGAVFSVGVTSLPK
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFPKESHGFVNGIYGVGNAGTAITSFLAPVIATSVGWRTTVQCYLILLAAFALMNFLLGD
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
RKEKKVNTPLMEQIKGVYKNEKLWFLCIFYFLTFGSFVAFTVYLPNFLVSHFGLEKVDAG
CCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
IRTAGFIVLATIMRPIGGWLGDKFNPFKILIFVFIGLTLSGIILSFMPSMNVYTFGCLLV
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
AFCAGIGNGTIFKLVPMYFSEQAGIVNGLVSALGGLGGFFPPLILTLLFQLTGHYAIGFM
HHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ALSEVALACLIITVWMYSQEKLLVMLKNH
HHHHHHHHHHHHHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]