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The map label for this gene is vanS [H]

Identifier: 222095532

GI number: 222095532

Start: 1848859

End: 1849959

Strand: Direct

Name: vanS [H]

Synonym: BCQ_1872

Alternate gene names: 222095532

Gene position: 1848859-1849959 (Clockwise)

Preceding gene: 222095531

Following gene: 222095533

Centisome position: 35.46

GC content: 30.88

Gene sequence:

>1101_bases
ATGTTTGGGGAGTCGGATATAAAATTGAAGATTAAATCATTACAAGGCATTAGAGCTAAGTTTTTTATCGCATTCATATG
TAGCATCTTGTTAGCGACTGTAAGTATAATAGTGTTTCAAATTTTGGTTGGAAATATATATAGTCAGGTTAATGTTTTAG
AGGAAAAATATTCATTTTTATATTTTATAGTCTTTTTGATTTTTACTACAACATACTTTGCATTCATGACAAAAACGCTG
ATGAAAAGGTTGTCTCAAATTAACAAGAACGTGAAAGAAATAAGTGAAGGTAATTTTGAAATACATATACCTATTTCGAA
AAGCGATGAGATTGGTGAACTAGCGGCAAATGTTAATAGAATGGCGAAAAGCTTAAAAGAGTCTATCGAGAATGAAAAAA
AATCACAGGAAATGAAAAATGAAATGATTAGTAATATTTCGCACGACTTACGAACACCTGTAACATCTTTAATTGGTTAC
GCGGACTTGTTAGGAAATAAGCTACATTCAAATGGAGAAGAATGTGAACAGTATGTAAGCATATTAAAGCGAAAAAGTTA
TGAATTAAAAAATCAAGTAGATGAATTATTAGAATACTGTCAAATAAATTATAGAGAGATTGAATTACATAAAAGTGTAG
TAAATATGAAAGCATTAATGGAACAAATTATGATTGATTTTGTACCACAACTAGATGATGCCAATATGAGCTTTTGTATG
AAAGGTGATAAGGAGTTACATGTGGAAATGGATGTAGCACTTATGGTGAGGTTATTCGAAAATGTAATTAGTAATAGTAT
TATGTACGGGAAAGACGGAAAGGAGATTGTAATTCAAATTTCTAAAAGAGACATGAATGTTGAAATAGAAATTAAAAATT
TTGGGCAATGTATTCCGAAAGAGGACCTTCCTTACGTTTTTGAGAAATTTTATCGTAGTGAAAAATCACGTAGTTCTCAT
ACGGGCGGGAAAGGAATAGGACTTGCAATTTCAAAAAGTATAGCCGATCTCCATCAAGGGGATATCACTGTACGTAGTAA
CGATAAAGAAACAGTTTTTACTGTGAAATTACCGCAGTATAAAAAAGTAAGAAAGTCGTAA

Upstream 100 bases:

>100_bases
AAAGAAAGGGCTTATGAAAGCAATAATACAGTAATGGTTCATATCGCAAGATTAAGAAATAAAATTGAAGAGAATCCAAA
AGAACCGAAGTTTATTCAAA

Downstream 100 bases:

>100_bases
GTATTTCTTTTATATGTCGTAATTTTTACTTTGTATCATTGAGAAAAAGGATATGAGAGTAGGGACGCGACATGTGGAAG
GCATTTTTATTAGTAATTAT

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MFGESDIKLKIKSLQGIRAKFFIAFICSILLATVSIIVFQILVGNIYSQVNVLEEKYSFLYFIVFLIFTTTYFAFMTKTL
MKRLSQINKNVKEISEGNFEIHIPISKSDEIGELAANVNRMAKSLKESIENEKKSQEMKNEMISNISHDLRTPVTSLIGY
ADLLGNKLHSNGEECEQYVSILKRKSYELKNQVDELLEYCQINYREIELHKSVVNMKALMEQIMIDFVPQLDDANMSFCM
KGDKELHVEMDVALMVRLFENVISNSIMYGKDGKEIVIQISKRDMNVEIEIKNFGQCIPKEDLPYVFEKFYRSEKSRSSH
TGGKGIGLAISKSIADLHQGDITVRSNDKETVFTVKLPQYKKVRKS

Sequences:

>Translated_366_residues
MFGESDIKLKIKSLQGIRAKFFIAFICSILLATVSIIVFQILVGNIYSQVNVLEEKYSFLYFIVFLIFTTTYFAFMTKTL
MKRLSQINKNVKEISEGNFEIHIPISKSDEIGELAANVNRMAKSLKESIENEKKSQEMKNEMISNISHDLRTPVTSLIGY
ADLLGNKLHSNGEECEQYVSILKRKSYELKNQVDELLEYCQINYREIELHKSVVNMKALMEQIMIDFVPQLDDANMSFCM
KGDKELHVEMDVALMVRLFENVISNSIMYGKDGKEIVIQISKRDMNVEIEIKNFGQCIPKEDLPYVFEKFYRSEKSRSSH
TGGKGIGLAISKSIADLHQGDITVRSNDKETVFTVKLPQYKKVRKS
>Mature_366_residues
MFGESDIKLKIKSLQGIRAKFFIAFICSILLATVSIIVFQILVGNIYSQVNVLEEKYSFLYFIVFLIFTTTYFAFMTKTL
MKRLSQINKNVKEISEGNFEIHIPISKSDEIGELAANVNRMAKSLKESIENEKKSQEMKNEMISNISHDLRTPVTSLIGY
ADLLGNKLHSNGEECEQYVSILKRKSYELKNQVDELLEYCQINYREIELHKSVVNMKALMEQIMIDFVPQLDDANMSFCM
KGDKELHVEMDVALMVRLFENVISNSIMYGKDGKEIVIQISKRDMNVEIEIKNFGQCIPKEDLPYVFEKFYRSEKSRSSH
TGGKGIGLAISKSIADLHQGDITVRSNDKETVFTVKLPQYKKVRKS

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=289, Percent_Identity=25.2595155709343, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1786783, Length=273, Percent_Identity=27.1062271062271, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786600, Length=222, Percent_Identity=25.6756756756757, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786912, Length=236, Percent_Identity=26.6949152542373, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI145693157, Length=244, Percent_Identity=23.7704918032787, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789149, Length=255, Percent_Identity=22.3529411764706, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87082128, Length=321, Percent_Identity=20.8722741433022, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1787894, Length=308, Percent_Identity=23.0519480519481, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI48994928, Length=235, Percent_Identity=27.2340425531915, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788279, Length=337, Percent_Identity=23.1454005934718, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790346, Length=309, Percent_Identity=22.3300970873786, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788549, Length=221, Percent_Identity=24.8868778280543, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788713, Length=248, Percent_Identity=22.5806451612903, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790861, Length=227, Percent_Identity=21.5859030837004, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790551, Length=311, Percent_Identity=21.8649517684887, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42036; Mature: 42036

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGESDIKLKIKSLQGIRAKFFIAFICSILLATVSIIVFQILVGNIYSQVNVLEEKYSFL
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFIVFLIFTTTYFAFMTKTLMKRLSQINKNVKEISEGNFEIHIPISKSDEIGELAANVNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHH
MAKSLKESIENEKKSQEMKNEMISNISHDLRTPVTSLIGYADLLGNKLHSNGEECEQYVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHH
ILKRKSYELKNQVDELLEYCQINYREIELHKSVVNMKALMEQIMIDFVPQLDDANMSFCM
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
KGDKELHVEMDVALMVRLFENVISNSIMYGKDGKEIVIQISKRDMNVEIEIKNFGQCIPK
CCCCEEEEHHHHHHHHHHHHHHHHCCEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCC
EDLPYVFEKFYRSEKSRSSHTGGKGIGLAISKSIADLHQGDITVRSNDKETVFTVKLPQY
CCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCH
KKVRKS
HHHHCC
>Mature Secondary Structure
MFGESDIKLKIKSLQGIRAKFFIAFICSILLATVSIIVFQILVGNIYSQVNVLEEKYSFL
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFIVFLIFTTTYFAFMTKTLMKRLSQINKNVKEISEGNFEIHIPISKSDEIGELAANVNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHH
MAKSLKESIENEKKSQEMKNEMISNISHDLRTPVTSLIGYADLLGNKLHSNGEECEQYVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHH
ILKRKSYELKNQVDELLEYCQINYREIELHKSVVNMKALMEQIMIDFVPQLDDANMSFCM
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
KGDKELHVEMDVALMVRLFENVISNSIMYGKDGKEIVIQISKRDMNVEIEIKNFGQCIPK
CCCCEEEEHHHHHHHHHHHHHHHHCCEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCC
EDLPYVFEKFYRSEKSRSSHTGGKGIGLAISKSIADLHQGDITVRSNDKETVFTVKLPQY
CCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCH
KKVRKS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]