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The map label for this gene is emrB [C]

Identifier: 222095493

GI number: 222095493

Start: 1804440

End: 1806188

Strand: Direct

Name: emrB [C]

Synonym: BCQ_1833

Alternate gene names: 222095493

Gene position: 1804440-1806188 (Clockwise)

Preceding gene: 222095492

Following gene: 222095494

Centisome position: 34.61

GC content: 32.76

Gene sequence:

>1749_bases
ATGGAACAATCAAAGGGTATTAAGATTGTCTTTTTAATGTGCTTAGGTATATTTCTTTGTATGATTGATACGACAATTAT
GAATATAGCTTTACCAGCGATACAAACGAGTGTAAATACTTCATTGGAAAAAATGTCTTGGGTATTAAATGTTTATACAA
TGACAATAGCAGTACTTGCTATCCCGCTAGGGCGGATAGCTGATATCTTTGGAAAAGCAAAGATGTATATTCTTGGTCTG
GTGATTTTTGGTGGCGGGTCAGTACTGTGCGCTTTTGCTAATACGGGGGATTTTCTTATCTTTTCTCGCTTTATACAAAG
TGTAGGGGCAGCAATTTTATTTCCAACTAGTATGGTAATTGGTGTATCAGCTATGCCGTTAGCTAAGAGGCATGTTGCAC
TAGCAATTTTAGGAGTAACACAAGGATTATCAGCGGCGATGGGTCCAGTAATAGGTGGGATTATTACACAAAATTTCGGT
TGGAGATGGGTGTTCTTCGTAAATGTTCCAATTTGTATTCTTGGAATCATTTTATGCTGTATTATGCTACAAATAAAAAA
TGAAGAACGTATTACCTCAAAAATAGATTGGATAGGATTGCTATTAAGTAGCATAGCAATTTTTTCTTTTACTCTCATAT
TAGTAAAAGGTAATACATGGGGATGGCAAAGTAATATAGCTTTGTTTTGTTATGCAATTAGCACTATTTCTCTTATTCTA
TTTGTTTTAGTAGAACAAAAGATTCATAATCCGATGGTGAATTTAAATTTATTTAAAGATCGAATGTTTGTCGGAGCATC
TATCGTTGTCATATTAAGTAATGTATTTTTAATTGGAGTTACTGTATTGCTTCCGACGTTCTTGACGAAAATACAAGGTC
GAACGGAAATTGAAGCAGCTTTTTTAGTGACACCTATTTCAGCAATGATATTTTTTGTCTCACCAGCTGCAGCAACTTTA
ATTAAAAAACTCGGAAAAGTAACTATTATTTTGTCAGGCTTTCTTGTTATGGGCTTATCTTATTATTGGTTGCAAATGAT
TGATGTTAATTCAACAAATATAGAAATTATTATTCCATGTATGATATTGGGTGTTGGGTATGGTTTAGTAGTAGGTCCAA
TTACAGTTTTAAGTGCTTCTTCGTTTGAAGGGGAATTATTAACTGCTTCTCAAAGTGTTGTATCAATGTTGCGGCAAGTA
GGTATTGTTTTAGCGGTCGCAATATTTGTATCTAACTTAACTCACAACTTAACTGTAAATAAAGAGAATGTATATCGTTA
TGCAGAAGAAAAGGTACGTAATATTCATGTAGGTAATACTCAGCAAGCGGAGATATTACAAATGACAAAAGAAAAAATAG
AGAATCAAAGTATAGAAACGAATGTAGAAAAGCATCAAAATGAAACATTTGTAGGGTTACATAAAGAGAAAAAAGACGAA
TTAATTCACCAAAAAGTAGATGAAGTGTTAAATGCTGTTCCAGTAGAATATAGAGATATAAAAAGAGAAGAAGTTATGAA
TCAAGTGACAAATGAAGTTGAAAAACAAGAGGAAAGTATAAAGAAAGAAGTACTCGCATTTTCGAATGATGTAAACCATT
ATGCTAAGAATCAGATGGCGATGAGTTTTACAGATTTATATAAAGCGAGTGTGCCAATTATTTTAATTTGTGCTTTAGTA
AGTTTGTTATTTTCGGAAAGAAAGGTTTTGAGTAAAAAGAAAAGTGGAAGAGTAGTAGAGGAAGTGTAA

Upstream 100 bases:

>100_bases
TAGAGACTTTGAAAAGAAAACTAGAGCAACTAGAAGCAAAAGAACACAATAAAATGGAAGAGTGAAAAAACGTTCATAAT
AAATAAAGGGGTAGAAGAAC

Downstream 100 bases:

>100_bases
AGAAAACGGGCTAATAGCAGCCTGTTTTTTGTTTTTTGATTACATATTGTGAATGGTAGTATTTTTAATATAGCATGAGA
GAAGTCGAGTTCGGTTAGCA

Product: multidrug ABC transporter permease

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MEQSKGIKIVFLMCLGIFLCMIDTTIMNIALPAIQTSVNTSLEKMSWVLNVYTMTIAVLAIPLGRIADIFGKAKMYILGL
VIFGGGSVLCAFANTGDFLIFSRFIQSVGAAILFPTSMVIGVSAMPLAKRHVALAILGVTQGLSAAMGPVIGGIITQNFG
WRWVFFVNVPICILGIILCCIMLQIKNEERITSKIDWIGLLLSSIAIFSFTLILVKGNTWGWQSNIALFCYAISTISLIL
FVLVEQKIHNPMVNLNLFKDRMFVGASIVVILSNVFLIGVTVLLPTFLTKIQGRTEIEAAFLVTPISAMIFFVSPAAATL
IKKLGKVTIILSGFLVMGLSYYWLQMIDVNSTNIEIIIPCMILGVGYGLVVGPITVLSASSFEGELLTASQSVVSMLRQV
GIVLAVAIFVSNLTHNLTVNKENVYRYAEEKVRNIHVGNTQQAEILQMTKEKIENQSIETNVEKHQNETFVGLHKEKKDE
LIHQKVDEVLNAVPVEYRDIKREEVMNQVTNEVEKQEESIKKEVLAFSNDVNHYAKNQMAMSFTDLYKASVPIILICALV
SLLFSERKVLSKKKSGRVVEEV

Sequences:

>Translated_582_residues
MEQSKGIKIVFLMCLGIFLCMIDTTIMNIALPAIQTSVNTSLEKMSWVLNVYTMTIAVLAIPLGRIADIFGKAKMYILGL
VIFGGGSVLCAFANTGDFLIFSRFIQSVGAAILFPTSMVIGVSAMPLAKRHVALAILGVTQGLSAAMGPVIGGIITQNFG
WRWVFFVNVPICILGIILCCIMLQIKNEERITSKIDWIGLLLSSIAIFSFTLILVKGNTWGWQSNIALFCYAISTISLIL
FVLVEQKIHNPMVNLNLFKDRMFVGASIVVILSNVFLIGVTVLLPTFLTKIQGRTEIEAAFLVTPISAMIFFVSPAAATL
IKKLGKVTIILSGFLVMGLSYYWLQMIDVNSTNIEIIIPCMILGVGYGLVVGPITVLSASSFEGELLTASQSVVSMLRQV
GIVLAVAIFVSNLTHNLTVNKENVYRYAEEKVRNIHVGNTQQAEILQMTKEKIENQSIETNVEKHQNETFVGLHKEKKDE
LIHQKVDEVLNAVPVEYRDIKREEVMNQVTNEVEKQEESIKKEVLAFSNDVNHYAKNQMAMSFTDLYKASVPIILICALV
SLLFSERKVLSKKKSGRVVEEV
>Mature_582_residues
MEQSKGIKIVFLMCLGIFLCMIDTTIMNIALPAIQTSVNTSLEKMSWVLNVYTMTIAVLAIPLGRIADIFGKAKMYILGL
VIFGGGSVLCAFANTGDFLIFSRFIQSVGAAILFPTSMVIGVSAMPLAKRHVALAILGVTQGLSAAMGPVIGGIITQNFG
WRWVFFVNVPICILGIILCCIMLQIKNEERITSKIDWIGLLLSSIAIFSFTLILVKGNTWGWQSNIALFCYAISTISLIL
FVLVEQKIHNPMVNLNLFKDRMFVGASIVVILSNVFLIGVTVLLPTFLTKIQGRTEIEAAFLVTPISAMIFFVSPAAATL
IKKLGKVTIILSGFLVMGLSYYWLQMIDVNSTNIEIIIPCMILGVGYGLVVGPITVLSASSFEGELLTASQSVVSMLRQV
GIVLAVAIFVSNLTHNLTVNKENVYRYAEEKVRNIHVGNTQQAEILQMTKEKIENQSIETNVEKHQNETFVGLHKEKKDE
LIHQKVDEVLNAVPVEYRDIKREEVMNQVTNEVEKQEESIKKEVLAFSNDVNHYAKNQMAMSFTDLYKASVPIILICALV
SLLFSERKVLSKKKSGRVVEEV

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=491, Percent_Identity=27.9022403258656, Blast_Score=169, Evalue=6e-43,
Organism=Escherichia coli, GI1788710, Length=470, Percent_Identity=27.2340425531915, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1790195, Length=404, Percent_Identity=25.990099009901, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI87081983, Length=359, Percent_Identity=24.2339832869081, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788392, Length=279, Percent_Identity=26.5232974910394, Blast_Score=90, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6322958, Length=205, Percent_Identity=30.7317073170732, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321663, Length=131, Percent_Identity=34.3511450381679, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325455, Length=237, Percent_Identity=24.0506329113924, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324954, Length=305, Percent_Identity=20.983606557377, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323735, Length=316, Percent_Identity=28.1645569620253, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319783, Length=151, Percent_Identity=29.8013245033113, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 64320; Mature: 64320

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQSKGIKIVFLMCLGIFLCMIDTTIMNIALPAIQTSVNTSLEKMSWVLNVYTMTIAVLA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPLGRIADIFGKAKMYILGLVIFGGGSVLCAFANTGDFLIFSRFIQSVGAAILFPTSMVI
HHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHH
GVSAMPLAKRHVALAILGVTQGLSAAMGPVIGGIITQNFGWRWVFFVNVPICILGIILCC
CCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHH
IMLQIKNEERITSKIDWIGLLLSSIAIFSFTLILVKGNTWGWQSNIALFCYAISTISLIL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
FVLVEQKIHNPMVNLNLFKDRMFVGASIVVILSNVFLIGVTVLLPTFLTKIQGRTEIEAA
HHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
FLVTPISAMIFFVSPAAATLIKKLGKVTIILSGFLVMGLSYYWLQMIDVNSTNIEIIIPC
HHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEHHH
MILGVGYGLVVGPITVLSASSFEGELLTASQSVVSMLRQVGIVLAVAIFVSNLTHNLTVN
HHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
KENVYRYAEEKVRNIHVGNTQQAEILQMTKEKIENQSIETNVEKHQNETFVGLHKEKKDE
HHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCHHCCHHHCCCCCEEECCHHHHHH
LIHQKVDEVLNAVPVEYRDIKREEVMNQVTNEVEKQEESIKKEVLAFSNDVNHYAKNQMA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
MSFTDLYKASVPIILICALVSLLFSERKVLSKKKSGRVVEEV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCC
>Mature Secondary Structure
MEQSKGIKIVFLMCLGIFLCMIDTTIMNIALPAIQTSVNTSLEKMSWVLNVYTMTIAVLA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPLGRIADIFGKAKMYILGLVIFGGGSVLCAFANTGDFLIFSRFIQSVGAAILFPTSMVI
HHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHH
GVSAMPLAKRHVALAILGVTQGLSAAMGPVIGGIITQNFGWRWVFFVNVPICILGIILCC
CCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHH
IMLQIKNEERITSKIDWIGLLLSSIAIFSFTLILVKGNTWGWQSNIALFCYAISTISLIL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
FVLVEQKIHNPMVNLNLFKDRMFVGASIVVILSNVFLIGVTVLLPTFLTKIQGRTEIEAA
HHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
FLVTPISAMIFFVSPAAATLIKKLGKVTIILSGFLVMGLSYYWLQMIDVNSTNIEIIIPC
HHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEHHH
MILGVGYGLVVGPITVLSASSFEGELLTASQSVVSMLRQVGIVLAVAIFVSNLTHNLTVN
HHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
KENVYRYAEEKVRNIHVGNTQQAEILQMTKEKIENQSIETNVEKHQNETFVGLHKEKKDE
HHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCHHCCHHHCCCCCEEECCHHHHHH
LIHQKVDEVLNAVPVEYRDIKREEVMNQVTNEVEKQEESIKKEVLAFSNDVNHYAKNQMA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
MSFTDLYKASVPIILICALVSLLFSERKVLSKKKSGRVVEEV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]