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The map label for this gene is rnjA [H]

Identifier: 222095332

GI number: 222095332

Start: 1649933

End: 1651606

Strand: Direct

Name: rnjA [H]

Synonym: BCQ_1672

Alternate gene names: 222095332

Gene position: 1649933-1651606 (Clockwise)

Preceding gene: 222095331

Following gene: 222095333

Centisome position: 31.64

GC content: 33.69

Gene sequence:

>1674_bases
TTGAATTCATTAAAAAACAACTTGTCTATCGTTGCTTTAGGTGGAGTAAATGAAATAGGGAAAAATATGTACGCTATTCA
ATATGAAAATGATATTGTCGTTATTGATTGTGGATCTAAATTTCCAGATGAAAGTTTATTAGGAATTGATTTAATAATTC
CAGACATCACATACTTACAAGAAAATAAAGAAAAAATTCGCGGGTTAGTCGTGACGCACGGACATGAAGACCATATTGGC
GGAATACCATATTTTTTAAAACAACTAAACGTACCAATTTATGCGACGAAGTTAACGTTAGGTTTAATTGAAATTAAATT
AAAAGAGCATAATCTTCAAAATGATACAGAATTAATCGTCATTCACGCAGAATCAGAAATTGATCTCGGTTCTATTAAGA
CGACATTTTTTAAAACGAATCATAGTATTCCAGATTGTCTCGGTATTGTGTTTCATACTCGAGAAGGTAATGTAGTACAT
ACTGGGGATTTTAAATTTGATTTAACACCAGTAAATAACCAACATCCTGATATTCATAAAATGGCTAAAATAGGTAGTGA
AGGTGTACTAGCTTTACTATCGGAAAGTACAAATGCAGAACGACCAGGTTTTACTCCATCAGAGAGATCGGTAGGAGAAC
GGATAGAGGAAATATTTATGAAAGCAAATAGAAAAGTAATTATTTCTACATTTGCTTCTAATGTGAATCGTGTTCAGCAA
ATAGTTGAAGCTTGTATAAAAACAAATCGAAAACTGGCTTTACTCGGCAGAAGTATGGTAAATGTAGTAGAAGTTGCTCT
AGAGAAAGGGTATCTAAATATTCCAGATGGTATGTTAATTGAAGCAAATGAGGTAAATCATTTAGATCCATCGCAGGTCG
CAATACTTTGTACAGGAAGTCAAGGAGAACCAATGGCAGCTTTAGCGCGTTTAGCTAGTGGAAACTATAGGCAAGTAGAT
GTTTTACCAGAAGATACAGTTATTATAGCTGCGACTCCTATTCCAGGAAATGAACGTAATGTTTCGCGAATTATCGATAA
CTTATTTGCATTAGGGGCAAGAGTGATTTACGGATCAGGCAGTTCTACTGGAGTCCATGTATCTGGCCATGCATATCAAG
AAGAATTAAAGTTAATGCTCACATTAATGAAGCCCAAATATTTTATCCCCGTTCATGGAGAGTTTAGAATGCTACATCAC
CATAGCTTGCTAGCGGAATCAATTGGTGTCGAAAAAGAAAATATCTTTATCGTTCGTAATGGAGATGTTGTAGATATAAG
TAATGAAGTGGCCGTTCAATCTAGAAAAATACAGGCTGGAAATATATATGTAGATGGATTAGGAATTGGTGATGTTGGAA
ATGCATTATTACGTGATCGAAAACAACTTTCAGAAGATGGAATGCTCGTTATAGTTATTACTTTTAATAAAGTGGATGGA
GAAATAATTTCAGGTCCTGATATTATTTCTCGTGGATTCGTCTATGTTCGTGATTCAGAAGAGTTTTTGAAGGAATTAAA
TAAATTAGCTGTTATTACAATTAATAATTTGAAGAAAGAGAATGTGAATAGTTGGGGTATTTTGAAAAGAGAAGTAAGAG
AGGCTTTAGGGAAGTTTATATATACTAATACGAAAAGAAAACCGATGATCCTTCCTATAATAATAGAGGTTTAA

Upstream 100 bases:

>100_bases
TTTTATATGTATGAAATAGTATGAAGTCCTTTTTTATTACAAATAATAAAGCCTAAATGCTATTTCACGAATTATAAATG
TGAAAGGGAGGTTTTATCAT

Downstream 100 bases:

>100_bases
GAGTTAATAAAAAGAATTCTCGTAGGGGAATTCTTTTTTGTTTTGAAGACAGAATGAACATATAATGAAATAAATAGAAA
TGGAAGGAAATAAAAGATGT

Product: zn-dependent hydrolase

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MNSLKNNLSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDITYLQENKEKIRGLVVTHGHEDHIG
GIPYFLKQLNVPIYATKLTLGLIEIKLKEHNLQNDTELIVIHAESEIDLGSIKTTFFKTNHSIPDCLGIVFHTREGNVVH
TGDFKFDLTPVNNQHPDIHKMAKIGSEGVLALLSESTNAERPGFTPSERSVGERIEEIFMKANRKVIISTFASNVNRVQQ
IVEACIKTNRKLALLGRSMVNVVEVALEKGYLNIPDGMLIEANEVNHLDPSQVAILCTGSQGEPMAALARLASGNYRQVD
VLPEDTVIIAATPIPGNERNVSRIIDNLFALGARVIYGSGSSTGVHVSGHAYQEELKLMLTLMKPKYFIPVHGEFRMLHH
HSLLAESIGVEKENIFIVRNGDVVDISNEVAVQSRKIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITFNKVDG
EIISGPDIISRGFVYVRDSEEFLKELNKLAVITINNLKKENVNSWGILKREVREALGKFIYTNTKRKPMILPIIIEV

Sequences:

>Translated_557_residues
MNSLKNNLSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDITYLQENKEKIRGLVVTHGHEDHIG
GIPYFLKQLNVPIYATKLTLGLIEIKLKEHNLQNDTELIVIHAESEIDLGSIKTTFFKTNHSIPDCLGIVFHTREGNVVH
TGDFKFDLTPVNNQHPDIHKMAKIGSEGVLALLSESTNAERPGFTPSERSVGERIEEIFMKANRKVIISTFASNVNRVQQ
IVEACIKTNRKLALLGRSMVNVVEVALEKGYLNIPDGMLIEANEVNHLDPSQVAILCTGSQGEPMAALARLASGNYRQVD
VLPEDTVIIAATPIPGNERNVSRIIDNLFALGARVIYGSGSSTGVHVSGHAYQEELKLMLTLMKPKYFIPVHGEFRMLHH
HSLLAESIGVEKENIFIVRNGDVVDISNEVAVQSRKIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITFNKVDG
EIISGPDIISRGFVYVRDSEEFLKELNKLAVITINNLKKENVNSWGILKREVREALGKFIYTNTKRKPMILPIIIEV
>Mature_557_residues
MNSLKNNLSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDITYLQENKEKIRGLVVTHGHEDHIG
GIPYFLKQLNVPIYATKLTLGLIEIKLKEHNLQNDTELIVIHAESEIDLGSIKTTFFKTNHSIPDCLGIVFHTREGNVVH
TGDFKFDLTPVNNQHPDIHKMAKIGSEGVLALLSESTNAERPGFTPSERSVGERIEEIFMKANRKVIISTFASNVNRVQQ
IVEACIKTNRKLALLGRSMVNVVEVALEKGYLNIPDGMLIEANEVNHLDPSQVAILCTGSQGEPMAALARLASGNYRQVD
VLPEDTVIIAATPIPGNERNVSRIIDNLFALGARVIYGSGSSTGVHVSGHAYQEELKLMLTLMKPKYFIPVHGEFRMLHH
HSLLAESIGVEKENIFIVRNGDVVDISNEVAVQSRKIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITFNKVDG
EIISGPDIISRGFVYVRDSEEFLKELNKLAVITINNLKKENVNSWGILKREVREALGKFIYTNTKRKPMILPIIIEV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61673; Mature: 61673

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLKNNLSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDITYLQ
CCCCCCCEEEEEECCHHHHCCCEEEEEECCCEEEEECCCCCCCCCCEEEEEEECCHHHHH
ENKEKIRGLVVTHGHEDHIGGIPYFLKQLNVPIYATKLTLGLIEIKLKEHNLQNDTELIV
CCHHHHCEEEEECCCCCCCCCHHHHHHHCCCCEEEEEEEEEEEEEEEECCCCCCCCEEEE
IHAESEIDLGSIKTTFFKTNHSIPDCLGIVFHTREGNVVHTGDFKFDLTPVNNQHPDIHK
EEECCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCEEECCCEEEEEEECCCCCCCHHH
MAKIGSEGVLALLSESTNAERPGFTPSERSVGERIEEIFMKANRKVIISTFASNVNRVQQ
HHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHH
IVEACIKTNRKLALLGRSMVNVVEVALEKGYLNIPDGMLIEANEVNHLDPSQVAILCTGS
HHHHHHHCCCEEEEHHHHHHHHHHHHHHCCEEECCCCCEEEECCCCCCCCCCEEEEEECC
QGEPMAALARLASGNYRQVDVLPEDTVIIAATPIPGNERNVSRIIDNLFALGARVIYGSG
CCCHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECC
SSTGVHVSGHAYQEELKLMLTLMKPKYFIPVHGEFRMLHHHSLLAESIGVEKENIFIVRN
CCCCEEEECHHHHHHHHHHHHHHCCCEEEEECCCEEEEHHHHHHHHHHCCCCCCEEEEEC
GDVVDISNEVAVQSRKIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITFNKVDG
CCEEECCCCCEEEEEEEEECCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
EIISGPDIISRGFVYVRDSEEFLKELNKLAVITINNLKKENVNSWGILKREVREALGKFI
CEECCCCHHHCCEEEEECCHHHHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHE
YTNTKRKPMILPIIIEV
EECCCCCCEEEEEEEEC
>Mature Secondary Structure
MNSLKNNLSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDITYLQ
CCCCCCCEEEEEECCHHHHCCCEEEEEECCCEEEEECCCCCCCCCCEEEEEEECCHHHHH
ENKEKIRGLVVTHGHEDHIGGIPYFLKQLNVPIYATKLTLGLIEIKLKEHNLQNDTELIV
CCHHHHCEEEEECCCCCCCCCHHHHHHHCCCCEEEEEEEEEEEEEEEECCCCCCCCEEEE
IHAESEIDLGSIKTTFFKTNHSIPDCLGIVFHTREGNVVHTGDFKFDLTPVNNQHPDIHK
EEECCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCEEECCCEEEEEEECCCCCCCHHH
MAKIGSEGVLALLSESTNAERPGFTPSERSVGERIEEIFMKANRKVIISTFASNVNRVQQ
HHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHH
IVEACIKTNRKLALLGRSMVNVVEVALEKGYLNIPDGMLIEANEVNHLDPSQVAILCTGS
HHHHHHHCCCEEEEHHHHHHHHHHHHHHCCEEECCCCCEEEECCCCCCCCCCEEEEEECC
QGEPMAALARLASGNYRQVDVLPEDTVIIAATPIPGNERNVSRIIDNLFALGARVIYGSG
CCCHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECC
SSTGVHVSGHAYQEELKLMLTLMKPKYFIPVHGEFRMLHHHSLLAESIGVEKENIFIVRN
CCCCEEEECHHHHHHHHHHHHHHCCCEEEEECCCEEEEHHHHHHHHHHCCCCCCEEEEEC
GDVVDISNEVAVQSRKIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITFNKVDG
CCEEECCCCCEEEEEEEEECCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
EIISGPDIISRGFVYVRDSEEFLKELNKLAVITINNLKKENVNSWGILKREVREALGKFI
CEECCCCHHHCCEEEEECCHHHHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHE
YTNTKRKPMILPIIIEV
EECCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]