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The map label for this gene is yjfC [C]

Identifier: 222095304

GI number: 222095304

Start: 1611222

End: 1612460

Strand: Direct

Name: yjfC [C]

Synonym: BCQ_1644

Alternate gene names: 222095304

Gene position: 1611222-1612460 (Clockwise)

Preceding gene: 222095303

Following gene: 222095305

Centisome position: 30.9

GC content: 35.35

Gene sequence:

>1239_bases
ATGTCGCAATACATAAGGGATCGAAAAGAGTTTTATTCAAAATATCAGGATTTCTGGTCGGATTTATATGAATGTGAGTA
TAGTTTGTTTCATGTTTTTCCTATAACAAATGAGACTATGAAACAATTACAAGTTGCAACTGAACGAATGGGGAAAATAT
TTTTTAAGACTGCTAGACTTTTGCGTAATTTAAGTGACGAACAGCTTCTTGAACTTGGATTTCCCCCCGCAAGCTTATCG
TTCATTAGAATGAAAGGATTATATCCTGAATCTGTTATTTCACGATTTGATTTCGTTTTAACAGGCGATAACCAAATTAA
AATGCTTGAGTTTAATAGTGATACACCTACCTTTATTATGGAATGCTTTCAAATGAATGGGGAAGTTTGTAAAAAGCTAG
GTTATGATGATCCAAACTTAGGCCAAGAGCGATTGCTATCCTCTGGTGTTACAAAAGCTGTAATGGAAGCGACAAAGGGA
ATAGACAACCCAAATGTTGTTTTTACAGCGCATCATGAGCATATTGAGGATTGGAATACGACTATGTATTTAAGTCAGTT
ATGCCGTGTTGAAAATAAAGTCGTGCCAATGTCAGAACTTAGAATTACAAAAGATTCTTTAGTGGATAGGGATGGAGTGC
AAATTGATGTTTTATATCGTCAAACATATCCGATAGAAGATTTAATTGAGGATCAAGATCCTGAAACAGGGGATTTGGTA
GGTGTGGAGTTGTTACAACTCGTAAAAGCCGGAAAGCTTTTTATTATAAATCCTTTGTCAGCATTTTTACTTCAGCCTAA
ATCAGTTCAATGTCTTATTTGGGGATTAGCAGAAGAAGAGGTCTTTTATACAAATGAGGAACGACAGTGGATTAAAGAAT
ATATGTTACCTACATATTTAGAACCAGATTTATTTTTAGGAAAAGGTTCTTTCGTTCGAAAACCTTCATTTGGTAGAGAG
GGAGACACGATTACCATTCGTGATAAGGACGAAAATATTATGATTCAAAATGCACACCAAACGTATAAAGCGTCACTGCC
GATATTTCAAAAGTATACAGAACTTCCAGTCGTAACTTTGGAAACAGAGAAAGGGATGGAGAAACTGTCGTATGTGTTTG
GTTCGTTTTTAATTGCAGGAAAAGCAAGTAGTATCGGTATTCGTGCTGGTGAAAAAATTACGGGGAATGAATCGTACTAT
TTACCAGTAGGTATAAACAAGGAGGAAGTATATGATTAA

Upstream 100 bases:

>100_bases
CACTTTCAAAAATTCATCGGCGTTTAAATCTTATCAATCGTCTGCGGAATTTAAAGGTGGAATTGGAAGCGGTTCAAAGG
GAGGATTTGGGGGCTAACAT

Downstream 100 bases:

>100_bases
TTTTTTATTATATTTAGCAGTAACACTTGGTCTTTTATGCATAGGTCTTTTCCTGATGGAAGTGACAACGAAAGTAAAAG
AATTTAAGTTAATGGCCCAA

Product: glutathionylspermidine synthase

Products: N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine; ADP; phosphate

Alternate protein names: Glutathionylspermidine Synthetase; Glutathionylspermidine Synthase-Like Protein; Glutathionylspermidine Synthase-Like

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MSQYIRDRKEFYSKYQDFWSDLYECEYSLFHVFPITNETMKQLQVATERMGKIFFKTARLLRNLSDEQLLELGFPPASLS
FIRMKGLYPESVISRFDFVLTGDNQIKMLEFNSDTPTFIMECFQMNGEVCKKLGYDDPNLGQERLLSSGVTKAVMEATKG
IDNPNVVFTAHHEHIEDWNTTMYLSQLCRVENKVVPMSELRITKDSLVDRDGVQIDVLYRQTYPIEDLIEDQDPETGDLV
GVELLQLVKAGKLFIINPLSAFLLQPKSVQCLIWGLAEEEVFYTNEERQWIKEYMLPTYLEPDLFLGKGSFVRKPSFGRE
GDTITIRDKDENIMIQNAHQTYKASLPIFQKYTELPVVTLETEKGMEKLSYVFGSFLIAGKASSIGIRAGEKITGNESYY
LPVGINKEEVYD

Sequences:

>Translated_412_residues
MSQYIRDRKEFYSKYQDFWSDLYECEYSLFHVFPITNETMKQLQVATERMGKIFFKTARLLRNLSDEQLLELGFPPASLS
FIRMKGLYPESVISRFDFVLTGDNQIKMLEFNSDTPTFIMECFQMNGEVCKKLGYDDPNLGQERLLSSGVTKAVMEATKG
IDNPNVVFTAHHEHIEDWNTTMYLSQLCRVENKVVPMSELRITKDSLVDRDGVQIDVLYRQTYPIEDLIEDQDPETGDLV
GVELLQLVKAGKLFIINPLSAFLLQPKSVQCLIWGLAEEEVFYTNEERQWIKEYMLPTYLEPDLFLGKGSFVRKPSFGRE
GDTITIRDKDENIMIQNAHQTYKASLPIFQKYTELPVVTLETEKGMEKLSYVFGSFLIAGKASSIGIRAGEKITGNESYY
LPVGINKEEVYD
>Mature_411_residues
SQYIRDRKEFYSKYQDFWSDLYECEYSLFHVFPITNETMKQLQVATERMGKIFFKTARLLRNLSDEQLLELGFPPASLSF
IRMKGLYPESVISRFDFVLTGDNQIKMLEFNSDTPTFIMECFQMNGEVCKKLGYDDPNLGQERLLSSGVTKAVMEATKGI
DNPNVVFTAHHEHIEDWNTTMYLSQLCRVENKVVPMSELRITKDSLVDRDGVQIDVLYRQTYPIEDLIEDQDPETGDLVG
VELLQLVKAGKLFIINPLSAFLLQPKSVQCLIWGLAEEEVFYTNEERQWIKEYMLPTYLEPDLFLGKGSFVRKPSFGREG
DTITIRDKDENIMIQNAHQTYKASLPIFQKYTELPVVTLETEKGMEKLSYVFGSFLIAGKASSIGIRAGEKITGNESYYL
PVGINKEEVYD

Specific function: Unknown

COG id: COG0754

COG function: function code E; Glutathionylspermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.1.8

Molecular weight: Translated: 47472; Mature: 47341

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQYIRDRKEFYSKYQDFWSDLYECEYSLFHVFPITNETMKQLQVATERMGKIFFKTARL
CCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
LRNLSDEQLLELGFPPASLSFIRMKGLYPESVISRFDFVLTGDNQIKMLEFNSDTPTFIM
HHCCCHHHHHHHCCCCCCCEEEEECCCCHHHHHHHCCEEEECCCEEEEEEECCCCHHHHH
ECFQMNGEVCKKLGYDDPNLGQERLLSSGVTKAVMEATKGIDNPNVVFTAHHEHIEDWNT
HHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHCHH
TMYLSQLCRVENKVVPMSELRITKDSLVDRDGVQIDVLYRQTYPIEDLIEDQDPETGDLV
HHHHHHHHHHHCCCCCHHHHEECHHHHCCCCCCEEEEEEECCCCHHHHHCCCCCCCCCHH
GVELLQLVKAGKLFIINPLSAFLLQPKSVQCLIWGLAEEEVFYTNEERQWIKEYMLPTYL
HHHHHHHHHCCCEEEECCHHHHHCCCCCCEEEEEECCCCCEEEECHHHHHHHHHCCCCCC
EPDLFLGKGSFVRKPSFGREGDTITIRDKDENIMIQNAHQTYKASLPIFQKYTELPVVTL
CCCEEECCCCCEECCCCCCCCCEEEEEECCCCEEEEECCHHHHCCCCHHHHHHCCCEEEE
ETEKGMEKLSYVFGSFLIAGKASSIGIRAGEKITGNESYYLPVGINKEEVYD
ECHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEECCCCEEEEECCCHHHCCC
>Mature Secondary Structure 
SQYIRDRKEFYSKYQDFWSDLYECEYSLFHVFPITNETMKQLQVATERMGKIFFKTARL
CHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
LRNLSDEQLLELGFPPASLSFIRMKGLYPESVISRFDFVLTGDNQIKMLEFNSDTPTFIM
HHCCCHHHHHHHCCCCCCCEEEEECCCCHHHHHHHCCEEEECCCEEEEEEECCCCHHHHH
ECFQMNGEVCKKLGYDDPNLGQERLLSSGVTKAVMEATKGIDNPNVVFTAHHEHIEDWNT
HHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHCHH
TMYLSQLCRVENKVVPMSELRITKDSLVDRDGVQIDVLYRQTYPIEDLIEDQDPETGDLV
HHHHHHHHHHHCCCCCHHHHEECHHHHCCCCCCEEEEEEECCCCHHHHHCCCCCCCCCHH
GVELLQLVKAGKLFIINPLSAFLLQPKSVQCLIWGLAEEEVFYTNEERQWIKEYMLPTYL
HHHHHHHHHCCCEEEECCHHHHHCCCCCCEEEEEECCCCCEEEECHHHHHHHHHCCCCCC
EPDLFLGKGSFVRKPSFGREGDTITIRDKDENIMIQNAHQTYKASLPIFQKYTELPVVTL
CCCEEECCCCCEECCCCCCCCCEEEEEECCCCEEEEECCHHHHCCCCHHHHHHCCCEEEE
ETEKGMEKLSYVFGSFLIAGKASSIGIRAGEKITGNESYYLPVGINKEEVYD
ECHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEECCCCEEEEECCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: gamma-L-glutamyl-L-cysteinyl-glycine; spermidine; ATP

Specific reaction: gamma-L-glutamyl-L-cysteinyl-glycine + spermidine + ATP = N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA