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The map label for this gene is pcnB [H]

Identifier: 222095266

GI number: 222095266

Start: 1572892

End: 1574085

Strand: Direct

Name: pcnB [H]

Synonym: BCQ_1606

Alternate gene names: 222095266

Gene position: 1572892-1574085 (Clockwise)

Preceding gene: 222095265

Following gene: 222095267

Centisome position: 30.17

GC content: 37.35

Gene sequence:

>1194_bases
ATGGAAAGATTTAAAAAAGCTAGTTCTATTATTGAGACTTTAAAGCAACAAGGGCATGAGGCTTACTTTGTCGGTGGAAG
CGTAAGAGACCTTATTATCGATAGGCCGATTGGAGATATTGATATTGCGACATCTGCTTTACCAGAAGAAGTGATGGCTA
TATTTCCAAGACATGTTCCTGTTGGTCTTGAACATGGAACTGTAATTGTAGTAGAAAATGGTGAGCCTTATGAGGTAACC
ACTTTTCGAACAGAGAGTGAATATGAAGACTTTCGAAGACCTAGTAGTGTTCAATTTGTTCGTTCATTAGAAGAGGATTT
AAAACGTCGTGATTTCACAATGAATGCAATTGCAATGACAGAAGAAGGAAAAATGGTTGATTTATTTGCTGGACAGGAAG
CGATTCAAAAGAGAGAAATTATGACAGTGGGAAATGCTGCGGATCGGTTTCAAGAAGATGCCCTGCGAATGATGCGTGGT
ATTCGGTTCGTAAGTACGTTAGGTTTTTCTTTAGAGACGAAAACGAAGCAAGCAATTGAAACATATGGACATTTGCTGGA
ACATATAGCAATTGAGAGAATTACAGTGGAGTTTGAAAAACTGTTAACTGGCACATATTGTGTGAAGGCTCTCAAAGAAT
TAGTAGAAACGAAACTATTTTCTCATCTACCATATTTACAGATGTCAGAAGAGAAATTGTTAAAAGCTACGCAGTATAAA
TGGGATTCTTTTGAAGCAGACATTGAGGCGTGGGCGTTTTTCTTATATTGTATCGGGGAAGAACATCCAGCTGTCTTTTT
ACGTCAATGGAAGTTTTCGAATAAAAAAATAAAAGATATCGTCGCAGTTTTATTAACAATCCGTAAGAGAAAGGAGAAGG
ATTGGGATACAGTTCTTCTTTATAAAACGGGAATTCATATAGCTGAAATGGCAGAAAGAGTATATGAAGCGATGATTGAA
AGCTATGATCATACAGCTGTTAACCGGGTACAAACGTTGTTTCAAGCATTGCCAATTAAGAATCGACAAGAGATGAATGT
GACTGGGAATGATTTGTTAAACTGGGCAAGTAAAAAGCCAGGTCCGTGGGTTGCTGAAATGATTCAAAAAATTGAAGAAG
CAATTGTACAAGGAAACGTAGTAAATGAGAAAGAGTGTATAAGGGAGTGGCTGCAAGAATGCAATCTACTATAA

Upstream 100 bases:

>100_bases
GGAGAGCGAGCAAGAGAAAGTGTTTACGAGCAGTTTCGCTCTGAAAAAATCGTCTCACAATATGAAACGATTTACTATGA
TGTACTAAGGGATGACAAAA

Downstream 100 bases:

>100_bases
GAAAGCAGTTATTGCAAGTTTTTTCGGAAGCGGATGGCGAGTTTGTATCTGGTCAAACGATTAGTGATAAACTTGGTTGC
TCAAGAACTGCTGTGTGGAA

Product: tRNA cca-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MERFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMAIFPRHVPVGLEHGTVIVVENGEPYEVT
TFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMTEEGKMVDLFAGQEAIQKREIMTVGNAADRFQEDALRMMRG
IRFVSTLGFSLETKTKQAIETYGHLLEHIAIERITVEFEKLLTGTYCVKALKELVETKLFSHLPYLQMSEEKLLKATQYK
WDSFEADIEAWAFFLYCIGEEHPAVFLRQWKFSNKKIKDIVAVLLTIRKRKEKDWDTVLLYKTGIHIAEMAERVYEAMIE
SYDHTAVNRVQTLFQALPIKNRQEMNVTGNDLLNWASKKPGPWVAEMIQKIEEAIVQGNVVNEKECIREWLQECNLL

Sequences:

>Translated_397_residues
MERFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMAIFPRHVPVGLEHGTVIVVENGEPYEVT
TFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMTEEGKMVDLFAGQEAIQKREIMTVGNAADRFQEDALRMMRG
IRFVSTLGFSLETKTKQAIETYGHLLEHIAIERITVEFEKLLTGTYCVKALKELVETKLFSHLPYLQMSEEKLLKATQYK
WDSFEADIEAWAFFLYCIGEEHPAVFLRQWKFSNKKIKDIVAVLLTIRKRKEKDWDTVLLYKTGIHIAEMAERVYEAMIE
SYDHTAVNRVQTLFQALPIKNRQEMNVTGNDLLNWASKKPGPWVAEMIQKIEEAIVQGNVVNEKECIREWLQECNLL
>Mature_397_residues
MERFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMAIFPRHVPVGLEHGTVIVVENGEPYEVT
TFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMTEEGKMVDLFAGQEAIQKREIMTVGNAADRFQEDALRMMRG
IRFVSTLGFSLETKTKQAIETYGHLLEHIAIERITVEFEKLLTGTYCVKALKELVETKLFSHLPYLQMSEEKLLKATQYK
WDSFEADIEAWAFFLYCIGEEHPAVFLRQWKFSNKKIKDIVAVLLTIRKRKEKDWDTVLLYKTGIHIAEMAERVYEAMIE
SYDHTAVNRVQTLFQALPIKNRQEMNVTGNDLLNWASKKPGPWVAEMIQKIEEAIVQGNVVNEKECIREWLQECNLL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=206, Percent_Identity=32.5242718446602, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI87081691, Length=230, Percent_Identity=32.6086956521739, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1789436, Length=190, Percent_Identity=32.1052631578947, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71995920, Length=268, Percent_Identity=29.8507462686567, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI281360112, Length=269, Percent_Identity=28.996282527881, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI21357337, Length=269, Percent_Identity=28.996282527881, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45912; Mature: 45912

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMAIFPRHVP
CCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCC
VGLEHGTVIVVENGEPYEVTTFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMT
CCCCCCEEEEEECCCCEEEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
EEGKMVDLFAGQEAIQKREIMTVGNAADRFQEDALRMMRGIRFVSTLGFSLETKTKQAIE
CCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
TYGHLLEHIAIERITVEFEKLLTGTYCVKALKELVETKLFSHLPYLQMSEEKLLKATQYK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHCC
WDSFEADIEAWAFFLYCIGEEHPAVFLRQWKFSNKKIKDIVAVLLTIRKRKEKDWDTVLL
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
YKTGIHIAEMAERVYEAMIESYDHTAVNRVQTLFQALPIKNRQEMNVTGNDLLNWASKKP
EECCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCC
GPWVAEMIQKIEEAIVQGNVVNEKECIREWLQECNLL
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MERFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMAIFPRHVP
CCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCC
VGLEHGTVIVVENGEPYEVTTFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMT
CCCCCCEEEEEECCCCEEEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
EEGKMVDLFAGQEAIQKREIMTVGNAADRFQEDALRMMRGIRFVSTLGFSLETKTKQAIE
CCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
TYGHLLEHIAIERITVEFEKLLTGTYCVKALKELVETKLFSHLPYLQMSEEKLLKATQYK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHCC
WDSFEADIEAWAFFLYCIGEEHPAVFLRQWKFSNKKIKDIVAVLLTIRKRKEKDWDTVLL
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
YKTGIHIAEMAERVYEAMIESYDHTAVNRVQTLFQALPIKNRQEMNVTGNDLLNWASKKP
EECCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCC
GPWVAEMIQKIEEAIVQGNVVNEKECIREWLQECNLL
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA