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The map label for this gene is rfbA [H]

Identifier: 222094942

GI number: 222094942

Start: 1272803

End: 1273540

Strand: Direct

Name: rfbA [H]

Synonym: BCQ_1280

Alternate gene names: 222094942

Gene position: 1272803-1273540 (Clockwise)

Preceding gene: 222094941

Following gene: 222094943

Centisome position: 24.41

GC content: 37.67

Gene sequence:

>738_bases
GTGAAAGGGATTATTTTAGCAGGCGGTACTGGATCGAGGTTATACCCAATAACGAAAGTAACGAATAAACATTTGCTTCC
TGTTGGACGTTATCCAATGATTTATCATGCGGTATATAAGCTGAAACAATGCGAGATTACAGATATTATGATTATTACAG
GTAAAGAGCATATGGGAGATGTTGTTAGTTTTTTAGGAAGCGGTCAAGAGTTTGGCGTGTCCTTTACGTATCGTGTGCAA
GATAAGGCTGGTGGAATTGCGCAAGCATTAGGGCTTTGTGAAGATTTCGTTGGGAATGATCGCATGGTAGTTATATTAGG
TGATAATATTTTTTCGGATGATATTCGTCCGTATGTTGAAGAGTTTGCAAATCAAAAAGAAGGTGCGAAAGTACTGCTGC
AATCTGTAGATGATCCGGAGAGATTTGGCGTAGCAAATATTCAAAACGGCAAAATAATTGAAATTGAAGAAAAGCCGAAA
GAGCCGAAAAGTTCCTATGCAGTCACAGGAATTTATTTGTATGATTCGAAAGTCTTTTCTTATATAAAAGAATTAAAACC
TTCCGCAAGGGGAGAGCTTGAAATTACAGATATCAATAATTGGTATTTAAAACGTGAGGTACTTACTTATAATGAAATGA
GCGGTTGGTGGACTGATGCAGGAACTCATGTTTCTCTTCAAAGGGCGAATATGTTAGCGCGGGATATAAACTTTGGTAAA
CAGTTTAACGGAGAATAG

Upstream 100 bases:

>100_bases
ACGGAGATGAATTTGATGCGGATACATATGAGAAAGATCGAAAAGTGTTTGTAGAACAGTATAAAGATATATTATCTCCA
TCATAAGGGAGAGATGCAGA

Downstream 100 bases:

>100_bases
GTGAGAATATGGAAGTTATAGAAACAAACTTTACCGACGCAAAATTGTTAGAACCGAGGTTATTTGGAGATGAGCGTGGC
TTTTTTACTGAAAGTTATAA

Product: glucose-1-phosphate thymidylyltransferase

Products: diphosphate; dTDPglucose

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MKGIILAGGTGSRLYPITKVTNKHLLPVGRYPMIYHAVYKLKQCEITDIMIITGKEHMGDVVSFLGSGQEFGVSFTYRVQ
DKAGGIAQALGLCEDFVGNDRMVVILGDNIFSDDIRPYVEEFANQKEGAKVLLQSVDDPERFGVANIQNGKIIEIEEKPK
EPKSSYAVTGIYLYDSKVFSYIKELKPSARGELEITDINNWYLKREVLTYNEMSGWWTDAGTHVSLQRANMLARDINFGK
QFNGE

Sequences:

>Translated_245_residues
MKGIILAGGTGSRLYPITKVTNKHLLPVGRYPMIYHAVYKLKQCEITDIMIITGKEHMGDVVSFLGSGQEFGVSFTYRVQ
DKAGGIAQALGLCEDFVGNDRMVVILGDNIFSDDIRPYVEEFANQKEGAKVLLQSVDDPERFGVANIQNGKIIEIEEKPK
EPKSSYAVTGIYLYDSKVFSYIKELKPSARGELEITDINNWYLKREVLTYNEMSGWWTDAGTHVSLQRANMLARDINFGK
QFNGE
>Mature_245_residues
MKGIILAGGTGSRLYPITKVTNKHLLPVGRYPMIYHAVYKLKQCEITDIMIITGKEHMGDVVSFLGSGQEFGVSFTYRVQ
DKAGGIAQALGLCEDFVGNDRMVVILGDNIFSDDIRPYVEEFANQKEGAKVLLQSVDDPERFGVANIQNGKIIEIEEKPK
EPKSSYAVTGIYLYDSKVFSYIKELKPSARGELEITDINNWYLKREVLTYNEMSGWWTDAGTHVSLQRANMLARDINFGK
QFNGE

Specific function: Catalyzes The Formation Of Dtdp-Glucose, From Dttp And Glucose 1-Phosphate, As Well As Its Pyrophosphorolysis. [C]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=227, Percent_Identity=26.8722466960352, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI11761619, Length=227, Percent_Identity=26.8722466960352, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1788351, Length=233, Percent_Identity=47.6394849785408, Blast_Score=205, Evalue=2e-54,
Organism=Escherichia coli, GI1790224, Length=241, Percent_Identity=46.8879668049792, Blast_Score=198, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI133931050, Length=228, Percent_Identity=24.1228070175439, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320148, Length=226, Percent_Identity=29.2035398230088, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21355443, Length=225, Percent_Identity=24, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24644084, Length=225, Percent_Identity=24, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: 2.7.7.24

Molecular weight: Translated: 27519; Mature: 27519

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIILAGGTGSRLYPITKVTNKHLLPVGRYPMIYHAVYKLKQCEITDIMIITGKEHMGD
CCCEEEECCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCHHHHH
VVSFLGSGQEFGVSFTYRVQDKAGGIAQALGLCEDFVGNDRMVVILGDNIFSDDIRPYVE
HHHHHCCCHHHCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
EFANQKEGAKVLLQSVDDPERFGVANIQNGKIIEIEEKPKEPKSSYAVTGIYLYDSKVFS
HHCCCCCCHHHHHHCCCCHHHCCEEECCCCCEEEECCCCCCCCCCEEEEEEEEECHHHHH
YIKELKPSARGELEITDINNWYLKREVLTYNEMSGWWTDAGTHVSLQRANMLARDINFGK
HHHHHCCCCCCCEEEEECCCEEEEEEEEEEECCCCEECCCCCEEEHHHHHHHHHHCCCCC
QFNGE
CCCCC
>Mature Secondary Structure
MKGIILAGGTGSRLYPITKVTNKHLLPVGRYPMIYHAVYKLKQCEITDIMIITGKEHMGD
CCCEEEECCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCHHHHH
VVSFLGSGQEFGVSFTYRVQDKAGGIAQALGLCEDFVGNDRMVVILGDNIFSDDIRPYVE
HHHHHCCCHHHCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
EFANQKEGAKVLLQSVDDPERFGVANIQNGKIIEIEEKPKEPKSSYAVTGIYLYDSKVFS
HHCCCCCCHHHHHHCCCCHHHCCEEECCCCCEEEECCCCCCCCCCEEEEEEEEECHHHHH
YIKELKPSARGELEITDINNWYLKREVLTYNEMSGWWTDAGTHVSLQRANMLARDINFGK
HHHHHCCCCCCCEEEEECCCEEEEEEEEEEECCCCEECCCCCEEEHHHHHHHHHHCCCCC
QFNGE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTTP; alpha-D-glucose 1-phosphate

Specific reaction: dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-glucose

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]