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The map label for this gene is oppA [H]

Identifier: 222094914

GI number: 222094914

Start: 1246925

End: 1248571

Strand: Direct

Name: oppA [H]

Synonym: BCQ_1252

Alternate gene names: 222094914

Gene position: 1246925-1248571 (Clockwise)

Preceding gene: 222094912

Following gene: 222094916

Centisome position: 23.91

GC content: 35.82

Gene sequence:

>1647_bases
ATGAAAAAAAAGATGCCAGTTTTTGTAGTATCAACAGTAGCAATGAGCATGATGTTAGGGGCATGTAGCTACCAAAAAGA
TGAGCCGCAAGCGAACGCAAAAGGGGATAGCGGGAAAAGCGGTGCGAAGCAAGTTATTAACTTAATTGAAACGCAAGAAA
TTCCAACGATGGATCCAGCGTTATCAGCTGATGCGGTTTCTTCAAGAGTAATGACTAATACGATGGAAGGCTTATACAGT
TTAGGAAAAGATGATAAGCTAGTTCCTGGTGTAGCAAAGGAATTTAAAAAGTCAGAAGACGGTAAAAAATATACATTTAC
ATTACGTGAAGACGCAAAATGGTCAAATGGTGAGCCTGTGACGGCAAAAGATTTCGTATACGCATGGCAAAGAGCGATTA
ACCCAGATACAGCTGCGAAATCAGCGTACATTATGTATGATATAAAAAATGCAGAGAAAATTAACAAAAAAGAGATGAGT
CCGGACCAATTAGGGGTAAAGGCGATTGATGATTATACATTAGAAGTGGAATTAGACAATTCCATTCCATACCTTGTAGA
TTTAATGGTATATCCAATCTTCTATCCTGTGAATGAAAAGTTCGTGAAAGAGCAAGGTACGAAGTTTGGTTTGGAAGCGA
ATACAACACTTTATAACGGACCATTTACATTAAGTGATTGGCAACATGAACGTAGCTTTAAAATGACGAAGAGCTCGTCA
TATTGGGATAATAAAGAAGTGAAGATTGAGGAAGTAAACTTTAATATTGTTAAAGATACGTCTACACCAATTAATTTATA
TGAAACAAACGCAGTCGATCGAGCAACGTTATTAGCGGAGTTCATCGATAAATATAAAGGAAAACCAGATTTCCAAACAG
TAGAAGATACATCTGTATTCTTCCTTCGTTTAAATCAAAAAGATCCAGCGTTAGCAAATAAAAATATTCGTAAAGCAATT
TCACTTGCTTTTGAACGTAAACCGTTCGTTGATACATTATTAAATAACGGTTCTAAGCCAGCAACAGGATTAATTCCTGA
TAACTTCATTAAAGGACCGGATAAAAAGGACTTCCGTGCTGTAAATGGAGATATTGTAAAACCAAACGTGAAAGAAGCGA
AGAAATATTGGGAAGCTGGTAAAAAAGAGCTTGGTAAAAATGAAATCGAATTAGAGTTATTAAACGAAGATGTTGAATTA
TCGAAGAAAACAGGGGAATATTTAAAAGGTGAGTTAGAAAAGAATTTACCAGGTTTAACAGTGAAAATTAAACAACAACC
ATTCGCGCAAAAACTAAAATTAGAAGATGCGGGCGATTTCGTCATGTCATTCTCTGGATGGAGTGCAGACTTCCCAGATC
CAATTACATATCTTGATATGTTTGTAACTGACGGATCACAAAATAAAATGAAATATTCTAATCCGAAATATGATGAGATC
ATCATGAAAGCAAAAAAAGATGGAAGCGATGTAAATGCTCGTTGGAAAAGCTTACTAGAAGCAGAGAAAATGTTACTTGA
TGACGCTGCGATTGTACCGGTATATCAACGTGGTAGAGCATACTTACAAAGAGATTCCATTAAAAATATGTACAACCATA
AATATGGTGGCGATTTAAGCTTTAAATGGGCGTCAGTAGAGAAATAA

Upstream 100 bases:

>100_bases
AAAAAATATATTTGTGTGTATAATATGAATTGTTAGAACATATATATATTTCTTTTTGTTTAAAAATGAAAGGGCTAACA
TTTTTGTGGAGGGTGGAAGT

Downstream 100 bases:

>100_bases
GAAAAAAGCAGGTAATTGCCTGCTTTTTTTAATACGTACGTTTCTTATAAATACCTTTCCCACCAACTTGGTTCCAGCCG
GATTGTACATCCAAACTTTT

Product: oligopeptide ABC transporter substrate-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MKKKMPVFVVSTVAMSMMLGACSYQKDEPQANAKGDSGKSGAKQVINLIETQEIPTMDPALSADAVSSRVMTNTMEGLYS
LGKDDKLVPGVAKEFKKSEDGKKYTFTLREDAKWSNGEPVTAKDFVYAWQRAINPDTAAKSAYIMYDIKNAEKINKKEMS
PDQLGVKAIDDYTLEVELDNSIPYLVDLMVYPIFYPVNEKFVKEQGTKFGLEANTTLYNGPFTLSDWQHERSFKMTKSSS
YWDNKEVKIEEVNFNIVKDTSTPINLYETNAVDRATLLAEFIDKYKGKPDFQTVEDTSVFFLRLNQKDPALANKNIRKAI
SLAFERKPFVDTLLNNGSKPATGLIPDNFIKGPDKKDFRAVNGDIVKPNVKEAKKYWEAGKKELGKNEIELELLNEDVEL
SKKTGEYLKGELEKNLPGLTVKIKQQPFAQKLKLEDAGDFVMSFSGWSADFPDPITYLDMFVTDGSQNKMKYSNPKYDEI
IMKAKKDGSDVNARWKSLLEAEKMLLDDAAIVPVYQRGRAYLQRDSIKNMYNHKYGGDLSFKWASVEK

Sequences:

>Translated_548_residues
MKKKMPVFVVSTVAMSMMLGACSYQKDEPQANAKGDSGKSGAKQVINLIETQEIPTMDPALSADAVSSRVMTNTMEGLYS
LGKDDKLVPGVAKEFKKSEDGKKYTFTLREDAKWSNGEPVTAKDFVYAWQRAINPDTAAKSAYIMYDIKNAEKINKKEMS
PDQLGVKAIDDYTLEVELDNSIPYLVDLMVYPIFYPVNEKFVKEQGTKFGLEANTTLYNGPFTLSDWQHERSFKMTKSSS
YWDNKEVKIEEVNFNIVKDTSTPINLYETNAVDRATLLAEFIDKYKGKPDFQTVEDTSVFFLRLNQKDPALANKNIRKAI
SLAFERKPFVDTLLNNGSKPATGLIPDNFIKGPDKKDFRAVNGDIVKPNVKEAKKYWEAGKKELGKNEIELELLNEDVEL
SKKTGEYLKGELEKNLPGLTVKIKQQPFAQKLKLEDAGDFVMSFSGWSADFPDPITYLDMFVTDGSQNKMKYSNPKYDEI
IMKAKKDGSDVNARWKSLLEAEKMLLDDAAIVPVYQRGRAYLQRDSIKNMYNHKYGGDLSFKWASVEK
>Mature_548_residues
MKKKMPVFVVSTVAMSMMLGACSYQKDEPQANAKGDSGKSGAKQVINLIETQEIPTMDPALSADAVSSRVMTNTMEGLYS
LGKDDKLVPGVAKEFKKSEDGKKYTFTLREDAKWSNGEPVTAKDFVYAWQRAINPDTAAKSAYIMYDIKNAEKINKKEMS
PDQLGVKAIDDYTLEVELDNSIPYLVDLMVYPIFYPVNEKFVKEQGTKFGLEANTTLYNGPFTLSDWQHERSFKMTKSSS
YWDNKEVKIEEVNFNIVKDTSTPINLYETNAVDRATLLAEFIDKYKGKPDFQTVEDTSVFFLRLNQKDPALANKNIRKAI
SLAFERKPFVDTLLNNGSKPATGLIPDNFIKGPDKKDFRAVNGDIVKPNVKEAKKYWEAGKKELGKNEIELELLNEDVEL
SKKTGEYLKGELEKNLPGLTVKIKQQPFAQKLKLEDAGDFVMSFSGWSADFPDPITYLDMFVTDGSQNKMKYSNPKYDEI
IMKAKKDGSDVNARWKSLLEAEKMLLDDAAIVPVYQRGRAYLQRDSIKNMYNHKYGGDLSFKWASVEK

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=539, Percent_Identity=29.4990723562152, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI87081878, Length=537, Percent_Identity=27.1880819366853, Blast_Score=204, Evalue=9e-54,
Organism=Escherichia coli, GI1789397, Length=510, Percent_Identity=26.8627450980392, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1787762, Length=481, Percent_Identity=23.4927234927235, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787052, Length=322, Percent_Identity=24.223602484472, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789887, Length=401, Percent_Identity=24.9376558603491, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1789966, Length=445, Percent_Identity=22.4719101123595, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 62012; Mature: 62012

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKMPVFVVSTVAMSMMLGACSYQKDEPQANAKGDSGKSGAKQVINLIETQEIPTMDPA
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
LSADAVSSRVMTNTMEGLYSLGKDDKLVPGVAKEFKKSEDGKKYTFTLREDAKWSNGEPV
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCC
TAKDFVYAWQRAINPDTAAKSAYIMYDIKNAEKINKKEMSPDQLGVKAIDDYTLEVELDN
CHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHCCCHHHCCCEEECCEEEEEEECC
SIPYLVDLMVYPIFYPVNEKFVKEQGTKFGLEANTTLYNGPFTLSDWQHERSFKMTKSSS
CCHHHHHHHHHHHEECCCHHHHHHCCCEECCCCCCEEECCCEECCCCCCCCCEEECCCCC
YWDNKEVKIEEVNFNIVKDTSTPINLYETNAVDRATLLAEFIDKYKGKPDFQTVEDTSVF
CCCCCCEEEEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
FLRLNQKDPALANKNIRKAISLAFERKPFVDTLLNNGSKPATGLIPDNFIKGPDKKDFRA
EEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCEE
VNGDIVKPNVKEAKKYWEAGKKELGKNEIELELLNEDVELSKKTGEYLKGELEKNLPGLT
CCCCEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCE
VKIKQQPFAQKLKLEDAGDFVMSFSGWSADFPDPITYLDMFVTDGSQNKMKYSNPKYDEI
EEECCCCCHHHHCCCCCCCEEEEECCCCCCCCCCHHEEEEEEECCCCCCCCCCCCCHHHH
IMKAKKDGSDVNARWKSLLEAEKMLLDDAAIVPVYQRGRAYLQRDSIKNMYNHKYGGDLS
HHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCE
FKWASVEK
EEECCCCC
>Mature Secondary Structure
MKKKMPVFVVSTVAMSMMLGACSYQKDEPQANAKGDSGKSGAKQVINLIETQEIPTMDPA
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
LSADAVSSRVMTNTMEGLYSLGKDDKLVPGVAKEFKKSEDGKKYTFTLREDAKWSNGEPV
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCC
TAKDFVYAWQRAINPDTAAKSAYIMYDIKNAEKINKKEMSPDQLGVKAIDDYTLEVELDN
CHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHCCCHHHCCCEEECCEEEEEEECC
SIPYLVDLMVYPIFYPVNEKFVKEQGTKFGLEANTTLYNGPFTLSDWQHERSFKMTKSSS
CCHHHHHHHHHHHEECCCHHHHHHCCCEECCCCCCEEECCCEECCCCCCCCCEEECCCCC
YWDNKEVKIEEVNFNIVKDTSTPINLYETNAVDRATLLAEFIDKYKGKPDFQTVEDTSVF
CCCCCCEEEEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
FLRLNQKDPALANKNIRKAISLAFERKPFVDTLLNNGSKPATGLIPDNFIKGPDKKDFRA
EEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCEE
VNGDIVKPNVKEAKKYWEAGKKELGKNEIELELLNEDVELSKKTGEYLKGELEKNLPGLT
CCCCEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCE
VKIKQQPFAQKLKLEDAGDFVMSFSGWSADFPDPITYLDMFVTDGSQNKMKYSNPKYDEI
EEECCCCCHHHHCCCCCCCEEEEECCCCCCCCCCHHEEEEEEECCCCCCCCCCCCCHHHH
IMKAKKDGSDVNARWKSLLEAEKMLLDDAAIVPVYQRGRAYLQRDSIKNMYNHKYGGDLS
HHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCE
FKWASVEK
EEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]