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The map label for this gene is lplA [H]

Identifier: 222094816

GI number: 222094816

Start: 1144227

End: 1145216

Strand: Direct

Name: lplA [H]

Synonym: BCQ_1154

Alternate gene names: 222094816

Gene position: 1144227-1145216 (Clockwise)

Preceding gene: 222094815

Following gene: 222094817

Centisome position: 21.94

GC content: 35.35

Gene sequence:

>990_bases
ATGTTATTTATTGATAATAAAGGGATTACAGATCCTAGAATAAACTTAGCGATTGAAGAATACTGTGTGAAGAATTTAGA
TATTAACGAAACATATTTACTTTTCTACATTAATGAACCTTCGATTATCATTGGTAAAAACCAAAACACAGTAGAAGAAA
TTAATGCAGATTACGTAAAAGAAAAAGGTATTCATGTCGTTCGTCGTCTATCAGGCGGAGGGGCAGTATATCATGATTTA
GGAAACTTAAACTTCAGCTTTATTACGAAAGATGATGGAGATAGTTTTAGCAACTTCAAAAAATTCACAGAGCCTGTAAC
GAAAGCGCTTGGTAAATTAGGTGTAAATGCAGAATTAAGTGGTCGTAACGACATTTTAGCTGAAGGCAGAAAAATTTCAG
GTAACGCGCAGTTCTCAACGAAAGGTCGTATGTTCAGTCACGGTACGTTACTATTTGACTCTGAAATCGATCACGTCGTA
TCAGCACTGAAAGTAAAAATGGATAAGATCCAATCAAAAGGAATTAAATCAATTCGTAGCCGCGTTGCGAATATTACAGA
ATTCTTAAACGAAAAAATGACGACAGAAGAGTTTAGACAACTTCTTCTAGAAACAATTTTCGAAGGAGAAACAGAAATTC
CAACGTACGAATTAACAGAAAATGATTGGAAAGAAATCCATAAACTTTCTGAAGAACGTTACCAAAATTGGGATTGGAAC
TACGGAAAATCTCCAAAGTTCAACTTACAACATTCACACCGCTTCCCAGTTGGACAAGTTGACGTTCGTCTTGAAGTGAA
AAAAGGAACAGTAACAGAATGCAAAATTTACGGTGACTTCTTCGGTTCACTAGATGTGCATGATATTGAAGAACGTCTAA
CAGGCGTACAATTTGATAAAGATGCATTCACTGCAGCTTTAGAAGGCGTAGATATCGCACGTTACTTCGGTAATATTACA
ACAGAAGATTTCTTACATTTGTTCTTCTAA

Upstream 100 bases:

>100_bases
AGGCAATCCAGCTGATTTAGTAACAGAAGCAAAACAAATTTTCAGTGGCCATATTACGCTAGCGCATAGTGGCTACGTAT
GGAACTCATGAGGTGATACG

Downstream 100 bases:

>100_bases
AAATAAGGGAGGCTACCGTAACTGGCAGCCTCTTTCTTTTTGCGTAGATTGTCACGTTTTGTCGGTAAGTCGATATATTT
TAAAAATCGCTGATATATTT

Product: lipoate-protein ligase a

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MLFIDNKGITDPRINLAIEEYCVKNLDINETYLLFYINEPSIIIGKNQNTVEEINADYVKEKGIHVVRRLSGGGAVYHDL
GNLNFSFITKDDGDSFSNFKKFTEPVTKALGKLGVNAELSGRNDILAEGRKISGNAQFSTKGRMFSHGTLLFDSEIDHVV
SALKVKMDKIQSKGIKSIRSRVANITEFLNEKMTTEEFRQLLLETIFEGETEIPTYELTENDWKEIHKLSEERYQNWDWN
YGKSPKFNLQHSHRFPVGQVDVRLEVKKGTVTECKIYGDFFGSLDVHDIEERLTGVQFDKDAFTAALEGVDIARYFGNIT
TEDFLHLFF

Sequences:

>Translated_329_residues
MLFIDNKGITDPRINLAIEEYCVKNLDINETYLLFYINEPSIIIGKNQNTVEEINADYVKEKGIHVVRRLSGGGAVYHDL
GNLNFSFITKDDGDSFSNFKKFTEPVTKALGKLGVNAELSGRNDILAEGRKISGNAQFSTKGRMFSHGTLLFDSEIDHVV
SALKVKMDKIQSKGIKSIRSRVANITEFLNEKMTTEEFRQLLLETIFEGETEIPTYELTENDWKEIHKLSEERYQNWDWN
YGKSPKFNLQHSHRFPVGQVDVRLEVKKGTVTECKIYGDFFGSLDVHDIEERLTGVQFDKDAFTAALEGVDIARYFGNIT
TEDFLHLFF
>Mature_329_residues
MLFIDNKGITDPRINLAIEEYCVKNLDINETYLLFYINEPSIIIGKNQNTVEEINADYVKEKGIHVVRRLSGGGAVYHDL
GNLNFSFITKDDGDSFSNFKKFTEPVTKALGKLGVNAELSGRNDILAEGRKISGNAQFSTKGRMFSHGTLLFDSEIDHVV
SALKVKMDKIQSKGIKSIRSRVANITEFLNEKMTTEEFRQLLLETIFEGETEIPTYELTENDWKEIHKLSEERYQNWDWN
YGKSPKFNLQHSHRFPVGQVDVRLEVKKGTVTECKIYGDFFGSLDVHDIEERLTGVQFDKDAFTAALEGVDIARYFGNIT
TEDFLHLFF

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=303, Percent_Identity=31.3531353135314, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI21729884, Length=303, Percent_Identity=31.3531353135314, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI21729882, Length=303, Percent_Identity=31.3531353135314, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI21729880, Length=303, Percent_Identity=31.3531353135314, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI21729878, Length=303, Percent_Identity=31.3531353135314, Blast_Score=128, Evalue=6e-30,
Organism=Escherichia coli, GI1790846, Length=307, Percent_Identity=34.2019543973941, Blast_Score=178, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI71981909, Length=244, Percent_Identity=35.2459016393443, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6322415, Length=283, Percent_Identity=30.7420494699647, Blast_Score=122, Evalue=6e-29,
Organism=Drosophila melanogaster, GI28573464, Length=283, Percent_Identity=31.8021201413428, Blast_Score=117, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 37622; Mature: 37622

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFIDNKGITDPRINLAIEEYCVKNLDINETYLLFYINEPSIIIGKNQNTVEEINADYVK
CEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCHHHHHCHHHHH
EKGIHVVRRLSGGGAVYHDLGNLNFSFITKDDGDSFSNFKKFTEPVTKALGKLGVNAELS
HCCHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEC
GRNDILAEGRKISGNAQFSTKGRMFSHGTLLFDSEIDHVVSALKVKMDKIQSKGIKSIRS
CCCCHHHCCCCCCCCCEECCCCCEEECCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
RVANITEFLNEKMTTEEFRQLLLETIFEGETEIPTYELTENDWKEIHKLSEERYQNWDWN
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCC
YGKSPKFNLQHSHRFPVGQVDVRLEVKKGTVTECKIYGDFFGSLDVHDIEERLTGVQFDK
CCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCH
DAFTAALEGVDIARYFGNITTEDFLHLFF
HHHHHHHCCHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MLFIDNKGITDPRINLAIEEYCVKNLDINETYLLFYINEPSIIIGKNQNTVEEINADYVK
CEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCHHHHHCHHHHH
EKGIHVVRRLSGGGAVYHDLGNLNFSFITKDDGDSFSNFKKFTEPVTKALGKLGVNAELS
HCCHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEC
GRNDILAEGRKISGNAQFSTKGRMFSHGTLLFDSEIDHVVSALKVKMDKIQSKGIKSIRS
CCCCHHHCCCCCCCCCEECCCCCEEECCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
RVANITEFLNEKMTTEEFRQLLLETIFEGETEIPTYELTENDWKEIHKLSEERYQNWDWN
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCC
YGKSPKFNLQHSHRFPVGQVDVRLEVKKGTVTECKIYGDFFGSLDVHDIEERLTGVQFDK
CCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCH
DAFTAALEGVDIARYFGNITTEDFLHLFF
HHHHHHHCCHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]