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The map label for this gene is rsbU [H]

Identifier: 222094732

GI number: 222094732

Start: 1061492

End: 1062634

Strand: Direct

Name: rsbU [H]

Synonym: BCQ_1070

Alternate gene names: 222094732

Gene position: 1061492-1062634 (Clockwise)

Preceding gene: 222094731

Following gene: 222094736

Centisome position: 20.36

GC content: 32.63

Gene sequence:

>1143_bases
GTGTCCATTTTAATTGTAGATGATAATCCGGTGAATATCTTTGTAATTAAGAAGATTTTAAAACAAGCTGGATATCAGGA
TCTTGTATCACTTAATTCTGCACAAGAGCTTTTCGAATACATCCATTTTGGAAAAGATTCTTCCAGGCATAATGAAATAG
ATTTAATACTATTAGATATTATGATGCCTGAAATTGATGGACTTGAAGTTTGTAGGCGATTACAAAAAGAAGAGAAGTTT
AAAGATATTCCTATTATTTTTGTAACAGCTTTAGAAGATGCGAATAAATTAGCTGAAGCACTTGATATAGGTGCAATGGA
TTATATTACAAAACCAATAAATAAGGTTGAATTGTTAGCGCGTATGCGTGTAGCACTACGCTTGAAATCGGAATTAAATT
GGCATAAAGAACAGGAAGAAAATCTTCGGAATGAACTAGATTTAGCTACGCAAGTACAAAGAAACTTATTAAGTAGCCCA
TTAAGAGAAGAACACATAAAAATCGAAGCAAGTTACTTACCTTCATTTAAACTAGCTGGAGATATGTATTACTGGTATAA
AATCGATGAAAACCGCTACGGTATTATATTATTAGATGTGATGGGACATGGTGTATCTGCTTCCTTAGTTTGCATGTTCA
TTTCGTCTGTATTACGTGAAACGATTAAATGTTTAATCGATCCAGAACTTGTCATGAAAGACTTAAATAAATATATGACT
CTTTTACATAATGAGAATGACAATATTCCATATTATTTTACAGCGATATACTTAGTAGTTAATACAGAAGATAGAACGAT
TGAATATGTAAATGCAGGACATCCTTCTGGATATGTTTTAGTTGATGAAACAAATGTAGTGGAACTAAATCGCGGGAGTT
GTGCGGTAGGATTTTTTGATGAGATAAAAGTTGAAAAGACAGTTATACCTTTTGAGAAAAATGCTCAAATCGTATTGTTT
ACAGATGGTGTACTTGAAGCAATAGCAAGTGATGAGTTTGAGGCTGAAGAGAAATTACGTAATTTTACAGAAAGAAAATG
GGGAGAGTTAGAAGAAGAGATAGAAGGGTTTTACAAGGAAGAACAGAAGGAAGCACAATCAGATGATATGTGTCTTATCA
TGATACAAGCGAATGCGAAATAA

Upstream 100 bases:

>100_bases
TTGAGTAGGAAATTTTCATTTTATAACTGAGTATGTAATACTAGAAAAATAGAAAATAATAATCGATTTACATTTTATAT
AAGAAAAGGGGAAATCACTT

Downstream 100 bases:

>100_bases
AAAGCGTATGAAATCCAAATAGAGGATTTCATACGCTTTTATTGTATTTTTTGATAGATTTGTTCGTGTGGATTAAATTG
CGTATAATGCGGCATGATAT

Product: sigma factor sigb regulation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSILIVDDNPVNIFVIKKILKQAGYQDLVSLNSAQELFEYIHFGKDSSRHNEIDLILLDIMMPEIDGLEVCRRLQKEEKF
KDIPIIFVTALEDANKLAEALDIGAMDYITKPINKVELLARMRVALRLKSELNWHKEQEENLRNELDLATQVQRNLLSSP
LREEHIKIEASYLPSFKLAGDMYYWYKIDENRYGIILLDVMGHGVSASLVCMFISSVLRETIKCLIDPELVMKDLNKYMT
LLHNENDNIPYYFTAIYLVVNTEDRTIEYVNAGHPSGYVLVDETNVVELNRGSCAVGFFDEIKVEKTVIPFEKNAQIVLF
TDGVLEAIASDEFEAEEKLRNFTERKWGELEEEIEGFYKEEQKEAQSDDMCLIMIQANAK

Sequences:

>Translated_380_residues
MSILIVDDNPVNIFVIKKILKQAGYQDLVSLNSAQELFEYIHFGKDSSRHNEIDLILLDIMMPEIDGLEVCRRLQKEEKF
KDIPIIFVTALEDANKLAEALDIGAMDYITKPINKVELLARMRVALRLKSELNWHKEQEENLRNELDLATQVQRNLLSSP
LREEHIKIEASYLPSFKLAGDMYYWYKIDENRYGIILLDVMGHGVSASLVCMFISSVLRETIKCLIDPELVMKDLNKYMT
LLHNENDNIPYYFTAIYLVVNTEDRTIEYVNAGHPSGYVLVDETNVVELNRGSCAVGFFDEIKVEKTVIPFEKNAQIVLF
TDGVLEAIASDEFEAEEKLRNFTERKWGELEEEIEGFYKEEQKEAQSDDMCLIMIQANAK
>Mature_379_residues
SILIVDDNPVNIFVIKKILKQAGYQDLVSLNSAQELFEYIHFGKDSSRHNEIDLILLDIMMPEIDGLEVCRRLQKEEKFK
DIPIIFVTALEDANKLAEALDIGAMDYITKPINKVELLARMRVALRLKSELNWHKEQEENLRNELDLATQVQRNLLSSPL
REEHIKIEASYLPSFKLAGDMYYWYKIDENRYGIILLDVMGHGVSASLVCMFISSVLRETIKCLIDPELVMKDLNKYMTL
LHNENDNIPYYFTAIYLVVNTEDRTIEYVNAGHPSGYVLVDETNVVELNRGSCAVGFFDEIKVEKTVIPFEKNAQIVLFT
DGVLEAIASDEFEAEEKLRNFTERKWGELEEEIEGFYKEEQKEAQSDDMCLIMIQANAK

Specific function: Positive regulator of sigma-B activity. Dephosphorylates rsbV in response to energy stress [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PP2C-like domain [H]

Homologues:

Organism=Escherichia coli, GI1789809, Length=139, Percent_Identity=36.6906474820144, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788394, Length=126, Percent_Identity=38.0952380952381, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87082012, Length=136, Percent_Identity=33.0882352941176, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789149, Length=113, Percent_Identity=33.6283185840708, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786784, Length=127, Percent_Identity=33.8582677165354, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786599, Length=79, Percent_Identity=39.2405063291139, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322044, Length=116, Percent_Identity=30.1724137931034, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR001932
- InterPro:   IPR010822 [H]

Pfam domain/function: PF00989 PAS; PF07228 SpoIIE [H]

EC number: =3.1.3.3 [H]

Molecular weight: Translated: 43840; Mature: 43709

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILIVDDNPVNIFVIKKILKQAGYQDLVSLNSAQELFEYIHFGKDSSRHNEIDLILLDI
CEEEEECCCCCCHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEEEEE
MMPEIDGLEVCRRLQKEEKFKDIPIIFVTALEDANKLAEALDIGAMDYITKPINKVELLA
CCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCCHHHHHHCCHHHHHHHH
RMRVALRLKSELNWHKEQEENLRNELDLATQVQRNLLSSPLREEHIKIEASYLPSFKLAG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCEEEEC
DMYYWYKIDENRYGIILLDVMGHGVSASLVCMFISSVLRETIKCLIDPELVMKDLNKYMT
CEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LLHNENDNIPYYFTAIYLVVNTEDRTIEYVNAGHPSGYVLVDETNVVELNRGSCAVGFFD
HHCCCCCCCCEEEEEEEEEEECCCCEEEEEECCCCCCEEEEECCCEEEECCCCEEEECHH
EIKVEKTVIPFEKNAQIVLFTDGVLEAIASDEFEAEEKLRNFTERKWGELEEEIEGFYKE
HHEEEEEEECCCCCCEEEEEECHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHH
EQKEAQSDDMCLIMIQANAK
HHHHCCCCCEEEEEEEECCC
>Mature Secondary Structure 
SILIVDDNPVNIFVIKKILKQAGYQDLVSLNSAQELFEYIHFGKDSSRHNEIDLILLDI
EEEEECCCCCCHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEEEEE
MMPEIDGLEVCRRLQKEEKFKDIPIIFVTALEDANKLAEALDIGAMDYITKPINKVELLA
CCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCCHHHHHHCCHHHHHHHH
RMRVALRLKSELNWHKEQEENLRNELDLATQVQRNLLSSPLREEHIKIEASYLPSFKLAG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCEEEEC
DMYYWYKIDENRYGIILLDVMGHGVSASLVCMFISSVLRETIKCLIDPELVMKDLNKYMT
CEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LLHNENDNIPYYFTAIYLVVNTEDRTIEYVNAGHPSGYVLVDETNVVELNRGSCAVGFFD
HHCCCCCCCCEEEEEEEEEEECCCCEEEEEECCCCCCEEEEECCCEEEECCCCEEEECHH
EIKVEKTVIPFEKNAQIVLFTDGVLEAIASDEFEAEEKLRNFTERKWGELEEEIEGFYKE
HHEEEEEEECCCCCCEEEEEECHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHH
EQKEAQSDDMCLIMIQANAK
HHHHCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 10632888 [H]