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The map label for this gene is sigB [H]

Identifier: 222094730

GI number: 222094730

Start: 1060032

End: 1060805

Strand: Direct

Name: sigB [H]

Synonym: BCQ_1068

Alternate gene names: 222094730

Gene position: 1060032-1060805 (Clockwise)

Preceding gene: 222094729

Following gene: 222094731

Centisome position: 20.33

GC content: 36.43

Gene sequence:

>774_bases
ATGGAAATCCAATCTCAACCTACAACTCTTACTAAAGAAGACGTTATGAAACTAATTGCAGAATTTCAACAAAATCAATG
TGATGAAGCGCAAGAAAGGTTAGTTGAGCATTATAAAAATCTCGTATATTCCATTGCATATCGCTATTCAAAAGGCGGGC
CGATGCATGAGGATATTATACAGGTGGGAATGTTAGGGCTCTTAGGCGCAATAAGAAGGTATGATTATTCGATAGGGAAT
GCCTTTGAGCCTTTTGCAATACCTACGATAGTAGGGGAAATAAAGAAGTATTTGCGTGATAAAACTTGGGGCATTCACGT
TCCAAGGCGAATTAAAGATTTAGGCGGAAAAATTAAACTTGCGATAGAGGAGCTAACAGATCGCCTGCAGCGTTCACCGA
AGATTATAGAGATTGCAGATCATTTAGGACTATCCGAGGAGGAAGTACTAGAAATTATGGATGCGAAAAATAATTATCGA
GTATCTTCCTTAGATGATGTAGTTGAAAATGCATCTGATGGAAGTTCGGTAGCGAGAATTGAATCTGTAGGTGAAGTAGA
GCAAGGATATGAAGAGACAGAAAGGCGTCTCGTTTTAAAGGATATTTTTAACGTATTAAATGATACGGAAAAGAGTGTTA
TTCATTATATATTTGGAGAAAATTTAAATCAAAAAGATACAGGGGAACGGTTAGGTATTTCACAAATGCACGTTTCTCGT
ATTAAAAGACAAGCAATAAGTAAATTGAAACAAGCCGCATTTTTAGATACATAA

Upstream 100 bases:

>100_bases
TATTTAATCAATACGTTAATGGATGATATACAAATCATGCATGATGAGGGCATGACAGTTTTAATGACAAAATATATACA
AAGAGAGCAGGTGGAGAATG

Downstream 100 bases:

>100_bases
AAAATTTAAAATAATGATGTTTAAAACATGAGAAAAGGGGTACGTATTAAGTATAGGAGGAGATATAAAAATGTCACACG
ATGTGAAAGAACTAATCGAA

Product: RNA polymerase sigma factor sigb

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MEIQSQPTTLTKEDVMKLIAEFQQNQCDEAQERLVEHYKNLVYSIAYRYSKGGPMHEDIIQVGMLGLLGAIRRYDYSIGN
AFEPFAIPTIVGEIKKYLRDKTWGIHVPRRIKDLGGKIKLAIEELTDRLQRSPKIIEIADHLGLSEEEVLEIMDAKNNYR
VSSLDDVVENASDGSSVARIESVGEVEQGYEETERRLVLKDIFNVLNDTEKSVIHYIFGENLNQKDTGERLGISQMHVSR
IKRQAISKLKQAAFLDT

Sequences:

>Translated_257_residues
MEIQSQPTTLTKEDVMKLIAEFQQNQCDEAQERLVEHYKNLVYSIAYRYSKGGPMHEDIIQVGMLGLLGAIRRYDYSIGN
AFEPFAIPTIVGEIKKYLRDKTWGIHVPRRIKDLGGKIKLAIEELTDRLQRSPKIIEIADHLGLSEEEVLEIMDAKNNYR
VSSLDDVVENASDGSSVARIESVGEVEQGYEETERRLVLKDIFNVLNDTEKSVIHYIFGENLNQKDTGERLGISQMHVSR
IKRQAISKLKQAAFLDT
>Mature_257_residues
MEIQSQPTTLTKEDVMKLIAEFQQNQCDEAQERLVEHYKNLVYSIAYRYSKGGPMHEDIIQVGMLGLLGAIRRYDYSIGN
AFEPFAIPTIVGEIKKYLRDKTWGIHVPRRIKDLGGKIKLAIEELTDRLQRSPKIIEIADHLGLSEEEVLEIMDAKNNYR
VSSLDDVVENASDGSSVARIESVGEVEQGYEETERRLVLKDIFNVLNDTEKSVIHYIFGENLNQKDTGERLGISQMHVSR
IKRQAISKLKQAAFLDT

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=222, Percent_Identity=29.2792792792793, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789098, Length=232, Percent_Identity=26.7241379310345, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789448, Length=230, Percent_Identity=22.6086956521739, Blast_Score=60, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014288
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29313; Mature: 29313

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]