Click here to switch to the map view.

The map label for this gene is cwlA [H]

Identifier: 222094623

GI number: 222094623

Start: 943699

End: 945438

Strand: Reverse

Name: cwlA [H]

Synonym: BCQ_0961

Alternate gene names: 222094623

Gene position: 945438-943699 (Counterclockwise)

Preceding gene: 222094628

Following gene: 222094621

Centisome position: 18.13

GC content: 37.18

Gene sequence:

>1740_bases
ATGAAAAAAAAGTTTTACAACATTTTAGCATTATCTTTGGCATTACAAACATTTGTAGCGCCTACCCATTCATTCGCAGA
AAGTATAGAAAAAACTGCGGAATTGTCTATGGATGAACAAGCTTTAAAAAGCATTGAAGAACATGCAAAACAGCATAGAC
ATGACGAAGAAAACGACCTTGAAATCTCGGGTGAATATCTTCTCCACTTATCAACAAAACTTCCACTATACAATTCAAAT
GACTTAAAAACGAAAACAAATATTGAAATTGCTGAACCAGTAGTAAAAGCAACGAAGAAAAAAGGGAATGCATATTACAT
ACAAACTACGTTTGGTGCTGGCTGGATTAATAAGGAAAGTAATAGTTTAGAAGTACAAGAGATTCATAAACTCTCGAATC
AAAAACTAATCGTAACAGAAGAAGCATCAGTATATTCCTTCCCTTTCCAATCGTTTAAAGAAGAAACGAAATTACAGCCA
CAAACAGTAACTCCTACTGAACAAGCTGGGAATTGGTTTAACATTCAAATGAACGGAACTGAGAAATGGATTTATGCTCC
TTCAGCTACATTCGACGGTACGAAAGCATCATTATTTGAAAAAACAGGTGCTATCGAAAAGAGAAGCGCAACTTTCTTCA
AAACACCATCTAATTTACCTACAAATAACGTTTATGGTGTTACTTTTAAAGAAATGATTGTTCCAAAAGGAAATGAAAAT
ATTCGCCCTGGCTACGCAATGGAACCGAAATACATTACAATTCATGAAACTGCTAATACAAGTAAAGGTGCGAACGCTTT
AAATCATGCGAAGTACTTAGATAATCAAGCTCGCGGAAATACTGATCGTTCAGCGTCTTGGCATTTTACAGTAGATGACA
AAGAAATTTATCAACATCTTCCGCTAAATGAAGTTGGGTGGCATGCTGGAGATAAAACTGGAAACTATGAATCTATCGGA
ATTGAAATTGCTGTGAATAGTGATGGAAATTATACGAAAGCGGTAGAAAATGCGCGCAAGTTAGCTGCTTTCTTAATGAA
TGAATTAAACATATCTCTAGATCACGTTCAAAAGCATCAATTTTGGAGCGGAAAAAACTGCCCTGCATTTATGATTCAGC
GCGGACAATGGGATGCTTTCTTAAAGGGCACGAACGCTTACTATAACGAACATCGTAAAGATGTTATGCCGCCGCCAGAA
GTCCCTCATGAAAAAGACGACATTGCAGGTGGTTGGTATGAGCAAGACATTCGTCAATTAGCTGCTAGAAAAATCATGTT
CGGTGACGGCAATGGTTCTTACTGGCCAAACCGCCTCGTGACACGAGCTGAGTTTGCAAATTTAATGTCGAGAGCTTTAA
AATTACCTGCTGGAAACGCTAAATTTACAGACTTAAACGAAGCACACCCATCTTTAATAGATGGAATTAACCGCGCCGCT
AGCGCAGGGATCATTAATGGTCGTGGGAATAATAAATTTGATCCCAATGCTACAATAACACGTGATGAAGCTGTTATTAT
GATTGACCGTGCATTAGAATATAACTGGATTTATAGAAAAGAAGTGAAATTACCATTCACTGACCAACACTTAGCATACG
ATAAAAAAGCATTACAAAATGTTTACGCATACGGAATTGTAAAAGGTAACGAACGAAATGAATTCGTACCAAAAGGCACA
GCAACACGCGCTGAAGCAGCCGCATTTTTAAATCGAATGCTTAAAGTTATTGAAGCTTAA

Upstream 100 bases:

>100_bases
TTGTTACAATCTAGAACGTATTAGTACTTGCTACTAATTTAAATACATATAAAGGAGGACTCTTAACACGCACGAAAGAC
TATCACGTGTTAAAACAACT

Downstream 100 bases:

>100_bases
ACAAAGAAAAACGTAGCCAAAAGCTACGTTTTTCCTTTATCGTTTCGGTGTATTTTTATGATTCTTCAAAAACGGAAGCG
GGAATTGCTTGCGGAAATGC

Product: N-acetylmuramoyl-l-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MKKKFYNILALSLALQTFVAPTHSFAESIEKTAELSMDEQALKSIEEHAKQHRHDEENDLEISGEYLLHLSTKLPLYNSN
DLKTKTNIEIAEPVVKATKKKGNAYYIQTTFGAGWINKESNSLEVQEIHKLSNQKLIVTEEASVYSFPFQSFKEETKLQP
QTVTPTEQAGNWFNIQMNGTEKWIYAPSATFDGTKASLFEKTGAIEKRSATFFKTPSNLPTNNVYGVTFKEMIVPKGNEN
IRPGYAMEPKYITIHETANTSKGANALNHAKYLDNQARGNTDRSASWHFTVDDKEIYQHLPLNEVGWHAGDKTGNYESIG
IEIAVNSDGNYTKAVENARKLAAFLMNELNISLDHVQKHQFWSGKNCPAFMIQRGQWDAFLKGTNAYYNEHRKDVMPPPE
VPHEKDDIAGGWYEQDIRQLAARKIMFGDGNGSYWPNRLVTRAEFANLMSRALKLPAGNAKFTDLNEAHPSLIDGINRAA
SAGIINGRGNNKFDPNATITRDEAVIMIDRALEYNWIYRKEVKLPFTDQHLAYDKKALQNVYAYGIVKGNERNEFVPKGT
ATRAEAAAFLNRMLKVIEA

Sequences:

>Translated_579_residues
MKKKFYNILALSLALQTFVAPTHSFAESIEKTAELSMDEQALKSIEEHAKQHRHDEENDLEISGEYLLHLSTKLPLYNSN
DLKTKTNIEIAEPVVKATKKKGNAYYIQTTFGAGWINKESNSLEVQEIHKLSNQKLIVTEEASVYSFPFQSFKEETKLQP
QTVTPTEQAGNWFNIQMNGTEKWIYAPSATFDGTKASLFEKTGAIEKRSATFFKTPSNLPTNNVYGVTFKEMIVPKGNEN
IRPGYAMEPKYITIHETANTSKGANALNHAKYLDNQARGNTDRSASWHFTVDDKEIYQHLPLNEVGWHAGDKTGNYESIG
IEIAVNSDGNYTKAVENARKLAAFLMNELNISLDHVQKHQFWSGKNCPAFMIQRGQWDAFLKGTNAYYNEHRKDVMPPPE
VPHEKDDIAGGWYEQDIRQLAARKIMFGDGNGSYWPNRLVTRAEFANLMSRALKLPAGNAKFTDLNEAHPSLIDGINRAA
SAGIINGRGNNKFDPNATITRDEAVIMIDRALEYNWIYRKEVKLPFTDQHLAYDKKALQNVYAYGIVKGNERNEFVPKGT
ATRAEAAAFLNRMLKVIEA
>Mature_579_residues
MKKKFYNILALSLALQTFVAPTHSFAESIEKTAELSMDEQALKSIEEHAKQHRHDEENDLEISGEYLLHLSTKLPLYNSN
DLKTKTNIEIAEPVVKATKKKGNAYYIQTTFGAGWINKESNSLEVQEIHKLSNQKLIVTEEASVYSFPFQSFKEETKLQP
QTVTPTEQAGNWFNIQMNGTEKWIYAPSATFDGTKASLFEKTGAIEKRSATFFKTPSNLPTNNVYGVTFKEMIVPKGNEN
IRPGYAMEPKYITIHETANTSKGANALNHAKYLDNQARGNTDRSASWHFTVDDKEIYQHLPLNEVGWHAGDKTGNYESIG
IEIAVNSDGNYTKAVENARKLAAFLMNELNISLDHVQKHQFWSGKNCPAFMIQRGQWDAFLKGTNAYYNEHRKDVMPPPE
VPHEKDDIAGGWYEQDIRQLAARKIMFGDGNGSYWPNRLVTRAEFANLMSRALKLPAGNAKFTDLNEAHPSLIDGINRAA
SAGIINGRGNNKFDPNATITRDEAVIMIDRALEYNWIYRKEVKLPFTDQHLAYDKKALQNVYAYGIVKGNERNEFVPKGT
ATRAEAAAFLNRMLKVIEA

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 65376; Mature: 65376

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKFYNILALSLALQTFVAPTHSFAESIEKTAELSMDEQALKSIEEHAKQHRHDEENDL
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCE
EISGEYLLHLSTKLPLYNSNDLKTKTNIEIAEPVVKATKKKGNAYYIQTTFGAGWINKES
EECCEEEEEEEEECCCCCCCCCCEECCCCHHHHHHHHHHCCCCEEEEEEECCCCEECCCC
NSLEVQEIHKLSNQKLIVTEEASVYSFPFQSFKEETKLQPQTVTPTEQAGNWFNIQMNGT
CCCHHHHHHHHCCCEEEEEECCCEEECCHHHHHHHHCCCCCCCCCHHHCCCEEEEEECCC
EKWIYAPSATFDGTKASLFEKTGAIEKRSATFFKTPSNLPTNNVYGVTFKEMIVPKGNEN
EEEEECCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEHHHEECCCCCCC
IRPGYAMEPKYITIHETANTSKGANALNHAKYLDNQARGNTDRSASWHFTVDDKEIYQHL
CCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHHC
PLNEVGWHAGDKTGNYESIGIEIAVNSDGNYTKAVENARKLAAFLMNELNISLDHVQKHQ
CCCHHCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
FWSGKNCPAFMIQRGQWDAFLKGTNAYYNEHRKDVMPPPEVPHEKDDIAGGWYEQDIRQL
CCCCCCCCEEEEECCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
AARKIMFGDGNGSYWPNRLVTRAEFANLMSRALKLPAGNAKFTDLNEAHPSLIDGINRAA
HHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
SAGIINGRGNNKFDPNATITRDEAVIMIDRALEYNWIYRKEVKLPFTDQHLAYDKKALQN
HCCEEECCCCCCCCCCCEEECCCEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
VYAYGIVKGNERNEFVPKGTATRAEAAAFLNRMLKVIEA
HHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKKFYNILALSLALQTFVAPTHSFAESIEKTAELSMDEQALKSIEEHAKQHRHDEENDL
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCE
EISGEYLLHLSTKLPLYNSNDLKTKTNIEIAEPVVKATKKKGNAYYIQTTFGAGWINKES
EECCEEEEEEEEECCCCCCCCCCEECCCCHHHHHHHHHHCCCCEEEEEEECCCCEECCCC
NSLEVQEIHKLSNQKLIVTEEASVYSFPFQSFKEETKLQPQTVTPTEQAGNWFNIQMNGT
CCCHHHHHHHHCCCEEEEEECCCEEECCHHHHHHHHCCCCCCCCCHHHCCCEEEEEECCC
EKWIYAPSATFDGTKASLFEKTGAIEKRSATFFKTPSNLPTNNVYGVTFKEMIVPKGNEN
EEEEECCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEHHHEECCCCCCC
IRPGYAMEPKYITIHETANTSKGANALNHAKYLDNQARGNTDRSASWHFTVDDKEIYQHL
CCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHHC
PLNEVGWHAGDKTGNYESIGIEIAVNSDGNYTKAVENARKLAAFLMNELNISLDHVQKHQ
CCCHHCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
FWSGKNCPAFMIQRGQWDAFLKGTNAYYNEHRKDVMPPPEVPHEKDDIAGGWYEQDIRQL
CCCCCCCCEEEEECCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
AARKIMFGDGNGSYWPNRLVTRAEFANLMSRALKLPAGNAKFTDLNEAHPSLIDGINRAA
HHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
SAGIINGRGNNKFDPNATITRDEAVIMIDRALEYNWIYRKEVKLPFTDQHLAYDKKALQN
HCCEEECCCCCCCCCCCEEECCCEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
VYAYGIVKGNERNEFVPKGTATRAEAAAFLNRMLKVIEA
HHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]