Click here to switch to the map view.

The map label for this gene is pphA [H]

Identifier: 222094322

GI number: 222094322

Start: 625382

End: 626086

Strand: Direct

Name: pphA [H]

Synonym: BCQ_0636

Alternate gene names: 222094322

Gene position: 625382-626086 (Clockwise)

Preceding gene: 222094321

Following gene: 222094323

Centisome position: 11.99

GC content: 35.6

Gene sequence:

>705_bases
ATGAAAAGAATTCTTGTTATTAGTGATATTCATGGCGAAATAGAAAAGTTCGAACAGTTATTAGAAGAAGCTCAATACGA
TGCAAAAAAGGATCAACTAATTTTATTAGGGGATTATGTAGATCGTGGTCCAAATGCACGTGCAGTAATTGAAAGGGTAA
AAGAGTTAAAAGAAGATGGAGCCCTCGTTCTAAAGGGAAATCATGAAGATATGATGATTAAGGCGTTAACGACTGATGAG
GAACGTTCATGGAACCATTGGGTAAAAAGAAACGGTGGAGATAAGACTTTATACAGCTATGGATTTGTAGAAGAAGATAT
TGTAGTTAATGAGGAAGACTTCCAAAAACCGATTTTACAATCGCATGTTTTAGAAGAACATGTAAAGTTTATTCAAGAAT
TAGATCATTACATTGAAACAGAAAAGTATATATTTGTACATGCTGGTGTTGAGCCATTGAAACGGGTTTCTGAATGTGAA
CCATATACGTTAATGTGGATCCGTAATGAGTTTCATAATGGATATAGCGGTGAAAAGGTTGTTGTATTTGGTCATACGGA
AACAAAAACGCTTCACGGTAGTGAGAATTGCGGCGTGTATTTTGGTAGTAATCGAATTATTGGAATTGATGGCGGTGCTG
TATATGGCGGCCAGCTAAATTGTTTAGAATTGCCGAGTAAAAAGGTGTACGTAATAAAAAGTTGA

Upstream 100 bases:

>100_bases
GTTGTAAGGATAATCGTTGTATCTGTAATAAATACGGTTTTAAAATACAATTCTGAAGTTTCTGTCGTTTTGAATTATAA
TTAGAAAGAGGGGTGATAAT

Downstream 100 bases:

>100_bases
AAGAGATAAGGAGAATCTTATTTCTTTCAACTTTTTATTAAGCAAAATTTAAGGAAGAGTTTTTATAATTAATCTTTAAA
ATTCTATATCAAGAAAAAGT

Product: serine/threonine protein phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKRILVISDIHGEIEKFEQLLEEAQYDAKKDQLILLGDYVDRGPNARAVIERVKELKEDGALVLKGNHEDMMIKALTTDE
ERSWNHWVKRNGGDKTLYSYGFVEEDIVVNEEDFQKPILQSHVLEEHVKFIQELDHYIETEKYIFVHAGVEPLKRVSECE
PYTLMWIRNEFHNGYSGEKVVVFGHTETKTLHGSENCGVYFGSNRIIGIDGGAVYGGQLNCLELPSKKVYVIKS

Sequences:

>Translated_234_residues
MKRILVISDIHGEIEKFEQLLEEAQYDAKKDQLILLGDYVDRGPNARAVIERVKELKEDGALVLKGNHEDMMIKALTTDE
ERSWNHWVKRNGGDKTLYSYGFVEEDIVVNEEDFQKPILQSHVLEEHVKFIQELDHYIETEKYIFVHAGVEPLKRVSECE
PYTLMWIRNEFHNGYSGEKVVVFGHTETKTLHGSENCGVYFGSNRIIGIDGGAVYGGQLNCLELPSKKVYVIKS
>Mature_234_residues
MKRILVISDIHGEIEKFEQLLEEAQYDAKKDQLILLGDYVDRGPNARAVIERVKELKEDGALVLKGNHEDMMIKALTTDE
ERSWNHWVKRNGGDKTLYSYGFVEEDIVVNEEDFQKPILQSHVLEEHVKFIQELDHYIETEKYIFVHAGVEPLKRVSECE
PYTLMWIRNEFHNGYSGEKVVVFGHTETKTLHGSENCGVYFGSNRIIGIDGGAVYGGQLNCLELPSKKVYVIKS

Specific function: Plays a key role in signaling protein misfolding via the CpxR/CPXA transducing system. It also modulates the phosphorylated status of many phosphoproteins in E.coli, some of which acting as major chaperones. Has been shown, in vitro, to act on Ser, Thr an

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPP phosphatase family. PP-1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081988, Length=230, Percent_Identity=26.0869565217391, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR006186 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: =3.1.3.16 [H]

Molecular weight: Translated: 26889; Mature: 26889

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00125 SER_THR_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRILVISDIHGEIEKFEQLLEEAQYDAKKDQLILLGDYVDRGPNARAVIERVKELKEDG
CCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCC
ALVLKGNHEDMMIKALTTDEERSWNHWVKRNGGDKTLYSYGFVEEDIVVNEEDFQKPILQ
EEEEECCCCCEEEEEECCCCCCCHHHHHHCCCCCCEEEECCCCCCCEEECHHHHHHHHHH
SHVLEEHVKFIQELDHYIETEKYIFVHAGVEPLKRVSECEPYTLMWIRNEFHNGYSGEKV
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCCEEEEEEEHHHCCCCCCCEE
VVFGHTETKTLHGSENCGVYFGSNRIIGIDGGAVYGGQLNCLELPSKKVYVIKS
EEEECCCCEEECCCCCCCEEECCCEEEEECCCEEECCEEEEEECCCCEEEEEEC
>Mature Secondary Structure
MKRILVISDIHGEIEKFEQLLEEAQYDAKKDQLILLGDYVDRGPNARAVIERVKELKEDG
CCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCC
ALVLKGNHEDMMIKALTTDEERSWNHWVKRNGGDKTLYSYGFVEEDIVVNEEDFQKPILQ
EEEEECCCCCEEEEEECCCCCCCHHHHHHCCCCCCEEEECCCCCCCEEECHHHHHHHHHH
SHVLEEHVKFIQELDHYIETEKYIFVHAGVEPLKRVSECEPYTLMWIRNEFHNGYSGEKV
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCCEEEEEEEHHHCCCCCCCEE
VVFGHTETKTLHGSENCGVYFGSNRIIGIDGGAVYGGQLNCLELPSKKVYVIKS
EEEECCCCEEECCCCCCCEEECCCEEEEECCCEEECCEEEEEECCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9130712; 9097040; 9278503 [H]