Click here to switch to the map view.

The map label for this gene is rocR [H]

Identifier: 222094227

GI number: 222094227

Start: 517776

End: 519179

Strand: Reverse

Name: rocR [H]

Synonym: BCQ_0520

Alternate gene names: 222094227

Gene position: 519179-517776 (Counterclockwise)

Preceding gene: 222094231

Following gene: 222094226

Centisome position: 9.96

GC content: 33.9

Gene sequence:

>1404_bases
ATGCGTACAGAAGAAATTAATTTAAAACAGATCATTGAAATGAATATGCTATATGAAACTTTGCTCAATGAACTCGATAT
CGGGATTCATATTATTAATGAGGAAGGTAAAACGATTATCTATAACCGTAAAATGATGGAAATTGAATCAATGGAGCGCT
CAGATGTGCTATATAAAAGTCCTTTAGAAGTATTCGCATTTGAGGAAAATAAAAATAGTACTCTTATAGAAGCATTAAAA
TTAGGAAAAACAAACAAAAATATAAAACAAACTTATTTTAACAATAAAGGGCAAGAAATTACGACGATTAACAATACTTT
CCCTATAATAGAAAATGGAAAAATTAAAGGCGCTATTGAAATTTCAAAAGAGATTAGCAATTCTAAACAAACAATGAAAA
CAAACCTTTCCCGAAAACAAAATAATAAGTTTACCTTTGATCACATAATTGGTGATTCTGAAGCCATTCAATCAATCATT
GCGGAAGGAAAAAGGGTAATTCGTACATCCTCCTCTATACTTCTCGTAGGAGAGACAGGCACTGGGAAAGAACTATTTGC
ACAAAGTATCCATAATGAAAGCCAGCGTTCAGGCAAACCATTTATTTCACAAAACTGTGCCGCTATACCTGATACGTTAA
TGGAAAGTTTATTATTTGGTACGAACCGAGGTGCATTTACAGGAGCTATCGATAAAGCCGGTTTATTTGAAGAAGCGAAC
GGAGGAACTTTGTTATTAGATGAGATTAATTCGTTAAGTCCAGCACTTCAAGCGAAGTTACTGCGAGCTATACAAGAAAA
AACAATACGAAGAATCGGAGGCACACAAGAAAAAGAAATTGATGTTCGTATTATAGCAACTATTAATGAAGACCCACTTG
AAGCTATGACACACAATCGATTGCGGGAAGACTTATATTACCGATTAAGCGTCGTTACTTTATGTCTCCCACCTTTACGT
GAACGAAAAGAAGATATTCCTGCTCTTGTTCAGCACTTTATCGAAAAGTACAACATTCAATTTGGACTCGGCGTAACAGA
TGTAGATATACATGTAAGAGAATTCCTGTATGCATATGATTGGCCTGGAAATGTACGAGAATTGGAACATGTCATTGAAG
GTTCAATGAATTTAGTTGAAGATGAACATATCATTACAGCGTTTCATCTTCCCAATCGCTTTCGCGAACGAATAAAAAAA
GAATTCAATATGCAACCTCTCCTAACTAATAACGATACTGATGCGCCAAAAACGTTAAAGCACACAATAGAAGAAATGGA
AAAGAACTACATCAATCAAATTTTGAAAGAGTATCACGGTAATATTTCACAAGCTGCAAAGTTTTTAGGGTTAAGTAGAC
AAAACTTACAATATCGAATTAAAAAATTGCATTTACACATATGA

Upstream 100 bases:

>100_bases
TTAAAAAGAAAATAGCAAAAAAATTTTGCGGTTTTATAAGAATTTTAATAAAATTATAAAAAGTCAGTCTTCATAGGGTG
AAATGAGGAGAAGATAATCT

Downstream 100 bases:

>100_bases
AGTGAATGTTTCTCCAAGTGCTAGAAATGGGAAAATATGTTACAATAAACATATAACATACTAGAAAGGAACGATATATA
TGAGTAATGATAACAAATTA

Product: arginine utilization regulatory protein rocr

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MRTEEINLKQIIEMNMLYETLLNELDIGIHIINEEGKTIIYNRKMMEIESMERSDVLYKSPLEVFAFEENKNSTLIEALK
LGKTNKNIKQTYFNNKGQEITTINNTFPIIENGKIKGAIEISKEISNSKQTMKTNLSRKQNNKFTFDHIIGDSEAIQSII
AEGKRVIRTSSSILLVGETGTGKELFAQSIHNESQRSGKPFISQNCAAIPDTLMESLLFGTNRGAFTGAIDKAGLFEEAN
GGTLLLDEINSLSPALQAKLLRAIQEKTIRRIGGTQEKEIDVRIIATINEDPLEAMTHNRLREDLYYRLSVVTLCLPPLR
ERKEDIPALVQHFIEKYNIQFGLGVTDVDIHVREFLYAYDWPGNVRELEHVIEGSMNLVEDEHIITAFHLPNRFRERIKK
EFNMQPLLTNNDTDAPKTLKHTIEEMEKNYINQILKEYHGNISQAAKFLGLSRQNLQYRIKKLHLHI

Sequences:

>Translated_467_residues
MRTEEINLKQIIEMNMLYETLLNELDIGIHIINEEGKTIIYNRKMMEIESMERSDVLYKSPLEVFAFEENKNSTLIEALK
LGKTNKNIKQTYFNNKGQEITTINNTFPIIENGKIKGAIEISKEISNSKQTMKTNLSRKQNNKFTFDHIIGDSEAIQSII
AEGKRVIRTSSSILLVGETGTGKELFAQSIHNESQRSGKPFISQNCAAIPDTLMESLLFGTNRGAFTGAIDKAGLFEEAN
GGTLLLDEINSLSPALQAKLLRAIQEKTIRRIGGTQEKEIDVRIIATINEDPLEAMTHNRLREDLYYRLSVVTLCLPPLR
ERKEDIPALVQHFIEKYNIQFGLGVTDVDIHVREFLYAYDWPGNVRELEHVIEGSMNLVEDEHIITAFHLPNRFRERIKK
EFNMQPLLTNNDTDAPKTLKHTIEEMEKNYINQILKEYHGNISQAAKFLGLSRQNLQYRIKKLHLHI
>Mature_467_residues
MRTEEINLKQIIEMNMLYETLLNELDIGIHIINEEGKTIIYNRKMMEIESMERSDVLYKSPLEVFAFEENKNSTLIEALK
LGKTNKNIKQTYFNNKGQEITTINNTFPIIENGKIKGAIEISKEISNSKQTMKTNLSRKQNNKFTFDHIIGDSEAIQSII
AEGKRVIRTSSSILLVGETGTGKELFAQSIHNESQRSGKPFISQNCAAIPDTLMESLLFGTNRGAFTGAIDKAGLFEEAN
GGTLLLDEINSLSPALQAKLLRAIQEKTIRRIGGTQEKEIDVRIIATINEDPLEAMTHNRLREDLYYRLSVVTLCLPPLR
ERKEDIPALVQHFIEKYNIQFGLGVTDVDIHVREFLYAYDWPGNVRELEHVIEGSMNLVEDEHIITAFHLPNRFRERIKK
EFNMQPLLTNNDTDAPKTLKHTIEEMEKNYINQILKEYHGNISQAAKFLGLSRQNLQYRIKKLHLHI

Specific function: Positive regulator of arginine catabolism. Controls the transcription of the two operons rocABC and rocDEF and probably acts by binding to the corresponding upstream activating sequences [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=348, Percent_Identity=41.3793103448276, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI1790437, Length=324, Percent_Identity=38.8888888888889, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI87082117, Length=348, Percent_Identity=38.5057471264368, Blast_Score=228, Evalue=9e-61,
Organism=Escherichia coli, GI1788905, Length=317, Percent_Identity=38.4858044164038, Blast_Score=218, Evalue=9e-58,
Organism=Escherichia coli, GI1789233, Length=338, Percent_Identity=38.7573964497041, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1789087, Length=322, Percent_Identity=39.7515527950311, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI87082152, Length=324, Percent_Identity=39.1975308641975, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1790299, Length=339, Percent_Identity=36.8731563421829, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1786524, Length=362, Percent_Identity=34.5303867403315, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1787583, Length=321, Percent_Identity=33.0218068535826, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI87081872, Length=327, Percent_Identity=33.6391437308869, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI87081858, Length=445, Percent_Identity=28.5393258426966, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1789828, Length=240, Percent_Identity=35.4166666666667, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF08448 PAS_4; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53514; Mature: 53514

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTEEINLKQIIEMNMLYETLLNELDIGIHIINEEGKTIIYNRKMMEIESMERSDVLYKS
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECHHHHHHHCCCCCCHHCC
PLEVFAFEENKNSTLIEALKLGKTNKNIKQTYFNNKGQEITTINNTFPIIENGKIKGAIE
CCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEECCEECEEECCCEEEEEE
ISKEISNSKQTMKTNLSRKQNNKFTFDHIIGDSEAIQSIIAEGKRVIRTSSSILLVGETG
EHHHHHCHHHHHHHHHHHHCCCCEEHHHHCCCHHHHHHHHHCCHHEEEECCCEEEEECCC
TGKELFAQSIHNESQRSGKPFISQNCAAIPDTLMESLLFGTNRGAFTGAIDKAGLFEEAN
CCHHHHHHHHHCHHHHCCCCCHHCCCCCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCCC
GGTLLLDEINSLSPALQAKLLRAIQEKTIRRIGGTQEKEIDVRIIATINEDPLEAMTHNR
CCEEEEECHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCHHHHHHHHH
LREDLYYRLSVVTLCLPPLRERKEDIPALVQHFIEKYNIQFGLGVTDVDIHVREFLYAYD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEECCCCEEHHHHHHHHHHHCC
WPGNVRELEHVIEGSMNLVEDEHIITAFHLPNRFRERIKKEFNMQPLLTNNDTDAPKTLK
CCCCHHHHHHHHHCCCCEECCCCEEEEEECCHHHHHHHHHHCCCCCEECCCCCCCHHHHH
HTIEEMEKNYINQILKEYHGNISQAAKFLGLSRQNLQYRIKKLHLHI
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRTEEINLKQIIEMNMLYETLLNELDIGIHIINEEGKTIIYNRKMMEIESMERSDVLYKS
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECHHHHHHHCCCCCCHHCC
PLEVFAFEENKNSTLIEALKLGKTNKNIKQTYFNNKGQEITTINNTFPIIENGKIKGAIE
CCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEECCEECEEECCCEEEEEE
ISKEISNSKQTMKTNLSRKQNNKFTFDHIIGDSEAIQSIIAEGKRVIRTSSSILLVGETG
EHHHHHCHHHHHHHHHHHHCCCCEEHHHHCCCHHHHHHHHHCCHHEEEECCCEEEEECCC
TGKELFAQSIHNESQRSGKPFISQNCAAIPDTLMESLLFGTNRGAFTGAIDKAGLFEEAN
CCHHHHHHHHHCHHHHCCCCCHHCCCCCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCCC
GGTLLLDEINSLSPALQAKLLRAIQEKTIRRIGGTQEKEIDVRIIATINEDPLEAMTHNR
CCEEEEECHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCHHHHHHHHH
LREDLYYRLSVVTLCLPPLRERKEDIPALVQHFIEKYNIQFGLGVTDVDIHVREFLYAYD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEECCCCEEHHHHHHHHHHHCC
WPGNVRELEHVIEGSMNLVEDEHIITAFHLPNRFRERIKKEFNMQPLLTNNDTDAPKTLK
CCCCHHHHHHHHHCCCCEECCCCEEEEEECCHHHHHHHHHHCCCCCEECCCCCCCHHHHH
HTIEEMEKNYINQILKEYHGNISQAAKFLGLSRQNLQYRIKKLHLHI
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113162; 9384377 [H]