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The map label for this gene is truA [H]

Identifier: 222094223

GI number: 222094223

Start: 514940

End: 515677

Strand: Direct

Name: truA [H]

Synonym: BCQ_0516

Alternate gene names: 222094223

Gene position: 514940-515677 (Clockwise)

Preceding gene: 222094222

Following gene: 222094224

Centisome position: 9.88

GC content: 34.28

Gene sequence:

>738_bases
ATGAACAATTATAAATTAACGATTCAGTACGATGGTGCACGTTTTAAAGGATGGCAACGTCTCGGTAATAACGATAATAC
GATTCAAGGAAAAATCGAAAGTGTCATTTCCGAAATGGTCGGAAAAGAAATTGAAATTATCGGTTGTAGTAGAACAGACG
CTGGTGTACATGCTTTGAATCAAGTTGCTAACTTTCAAAGTGATGAGAAGTTAGTGGAACATAAAGTGAAAAAATATTTA
AATCAATATTTGCCAAATGATATTAGCATTACAAATGTGGAAGAAGTACATGATCGATTCCATGCTCGTTACAATTCTAA
AGCAAAAACATATCTGTATAAAATTTGGAATGAAGAGCATACGAACCCATTTATGCGTAAATACAGCATGCATGTGAATA
AAAAATTAAATGTGAAAAGCATGAAAGAAGCTGCGAAACATTTAGTTGGTTCACATGACTTTACTGCTTTTTCAAATGCA
AAATCAAAGAAAAAGTCTATGGTGCGAGAAGTATATACACTTGATGTGATGGAAGAGGCAGGATTTGTACAAATTAGAGT
AAGTGGTAACGGATTCCTTCATAACATGGTACGAAAAATTGTTGGAGCATTAATTGAAGTTGGATTAGGACAATTAGATG
CAGAGGCGATTCCACACATTTTAGAGGCGAAACAACGTAATCAGATTAATTGCCTTGCTGAGGCAAGTGGTTTGTATTTG
GAGAATGTTGAGTTTTAA

Upstream 100 bases:

>100_bases
ATACAGCTCATGTAGAAAATGTAGTGAAGCTTGTTAGAAAGTAAAGTGTATTTATATTCCCTCACGATATGAGGGAATAT
TTTCTATGAAGGAGAATAAA

Downstream 100 bases:

>100_bases
TTATGGAATAGAATAAGCAGTTTGAAAAAATAGAATGTGTTTGTTAATAATTTACATGGATGCATGTGTAATTTATAATA
AAAATAGTAAGAAATTAGCG

Product: tRNA pseudouridine synthase a

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I 2; tRNA-uridine isomerase I 2 [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MNNYKLTIQYDGARFKGWQRLGNNDNTIQGKIESVISEMVGKEIEIIGCSRTDAGVHALNQVANFQSDEKLVEHKVKKYL
NQYLPNDISITNVEEVHDRFHARYNSKAKTYLYKIWNEEHTNPFMRKYSMHVNKKLNVKSMKEAAKHLVGSHDFTAFSNA
KSKKKSMVREVYTLDVMEEAGFVQIRVSGNGFLHNMVRKIVGALIEVGLGQLDAEAIPHILEAKQRNQINCLAEASGLYL
ENVEF

Sequences:

>Translated_245_residues
MNNYKLTIQYDGARFKGWQRLGNNDNTIQGKIESVISEMVGKEIEIIGCSRTDAGVHALNQVANFQSDEKLVEHKVKKYL
NQYLPNDISITNVEEVHDRFHARYNSKAKTYLYKIWNEEHTNPFMRKYSMHVNKKLNVKSMKEAAKHLVGSHDFTAFSNA
KSKKKSMVREVYTLDVMEEAGFVQIRVSGNGFLHNMVRKIVGALIEVGLGQLDAEAIPHILEAKQRNQINCLAEASGLYL
ENVEF
>Mature_245_residues
MNNYKLTIQYDGARFKGWQRLGNNDNTIQGKIESVISEMVGKEIEIIGCSRTDAGVHALNQVANFQSDEKLVEHKVKKYL
NQYLPNDISITNVEEVHDRFHARYNSKAKTYLYKIWNEEHTNPFMRKYSMHVNKKLNVKSMKEAAKHLVGSHDFTAFSNA
KSKKKSMVREVYTLDVMEEAGFVQIRVSGNGFLHNMVRKIVGALIEVGLGQLDAEAIPHILEAKQRNQINCLAEASGLYL
ENVEF

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=280, Percent_Identity=28.5714285714286, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1788657, Length=240, Percent_Identity=34.5833333333333, Blast_Score=155, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71985046, Length=266, Percent_Identity=28.1954887218045, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 27993; Mature: 27993

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNYKLTIQYDGARFKGWQRLGNNDNTIQGKIESVISEMVGKEIEIIGCSRTDAGVHALN
CCCEEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
QVANFQSDEKLVEHKVKKYLNQYLPNDISITNVEEVHDRFHARYNSKAKTYLYKIWNEEH
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHEECCCCC
TNPFMRKYSMHVNKKLNVKSMKEAAKHLVGSHDFTAFSNAKSKKKSMVREVYTLDVMEEA
CCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GFVQIRVSGNGFLHNMVRKIVGALIEVGLGQLDAEAIPHILEAKQRNQINCLAEASGLYL
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEHHCCCCEE
ENVEF
ECCCC
>Mature Secondary Structure
MNNYKLTIQYDGARFKGWQRLGNNDNTIQGKIESVISEMVGKEIEIIGCSRTDAGVHALN
CCCEEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
QVANFQSDEKLVEHKVKKYLNQYLPNDISITNVEEVHDRFHARYNSKAKTYLYKIWNEEH
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHEECCCCC
TNPFMRKYSMHVNKKLNVKSMKEAAKHLVGSHDFTAFSNAKSKKKSMVREVYTLDVMEEA
CCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GFVQIRVSGNGFLHNMVRKIVGALIEVGLGQLDAEAIPHILEAKQRNQINCLAEASGLYL
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEHHCCCCEE
ENVEF
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]