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The map label for this gene is ilvB [H]

Identifier: 222094093

GI number: 222094093

Start: 368728

End: 370215

Strand: Direct

Name: ilvB [H]

Synonym: BCQ_0386

Alternate gene names: 222094093

Gene position: 368728-370215 (Clockwise)

Preceding gene: 222094092

Following gene: 222094095

Centisome position: 7.07

GC content: 37.1

Gene sequence:

>1488_bases
ATGGCTGCCGCTTATGCTAAATACACAAATCAGCTAAGTGTGTGTATTGGTTGTAGCGGACCAGGTGCCACAAATCTTGT
AACAGGTGCTGCTAATGCTATGCGAGAGCATTTACCTGTTTTATTCATTACAGGTGCCGTACCTCTTAGTACGGTAGGTT
TGAATGCATCACAAGAATTAGATGTAGAACCAGTCTTCAGGCCTGTTACAAAATATAGCGTTACGGTTAATGATGTAAAG
GATGTACTTAAAGAAGTCGCACTAGCAATTGAAATTGCTATATCGGGAGTACCAGGACCTGTACATATTGCGATACCGAT
TGATATTCAACATGAAAATATAGAAAATGTTGAAATGCCACCACTTCCCAAAAGACTTCCTATTGTTCCTGATCTAGACA
AAATAAAAGAAACAGCTAGAGAATTGGTAAAAAGGGAGAAGGGTTATATTTTTACAGGACAGGGAATAAGAAACTCTGTT
CAGTTATTAATAGAGGTGGCAGAATTGCTGAATTGGCCAATTATAACAACTCCACAAGCTAAGGGATATATCCCTGAGGA
ACATCCGCTTTTAGTCGGAGTGTTTGGATTTGCAGGTCATGAGGCTGCCTCTGCATTGATTAATGAAGGGGATGGGAAAG
CATTATTAATTGTGGGCTCCAGTTTAGGGGAAACTGCGACAAATAATTATAATGGAAACTTAATAAAAGATCGTTTCTCT
GTTCAAATGGATTTTGACCAATCTGTCTTCAATCGGAAGTACAAAGTGGATATTCCAGTACTAGGCGATATCAATTTAAG
TTTATTAATGTTAATAGAAGAATTAAAGGCTTTAGGGTTGCAAATGGATCGCGCTGAATTTACCGTTGAAAAGAAGAAAG
AGGAAGAGGAAGAGAATATAGAGGAATATAATACGAAAAATGTTCTCCTAAACCTTCAAAAATGTTTACCGTCAACTACT
AGATATACCATTGATATTGGTGAATTTATGTCGTATGTTATTCATCACATGAAGGTCATAGATTTTGACTCCTATAATAT
TAATGTACATTTTGGTGCAATGGGAAGCGGAATTGGTTCTGCGATTGGTTCAAAACTGGCTGAACCGGAGCGTCCGGTTG
TATGTATTACTGGTGATGGCTGCTTTTTCATGCACGGGTTGGAAATATTGACGGCGAAGGAATATAATTTACCTATTTTA
TTTGTTGTGATGAATAATGCTCGTTTAGGAATGGTATATCACGGTCATTCATTACAATTCCGTAGAACTCATCCATCCTT
TGAACAGGAATCAATCAATATTAGTGCAATGGCCGCAGCTATGAATATACCAAGCTATAGAGTCAATGAATTACAAGATA
TCAATCAAGATGTTATAAACAACTTAATGAATGTAAATGGGCCAGCAATTCTAGAGGTTGCCTTAGTCGATAATAATACT
CCTCCTATGGGAGATAGAGTTAAATTTTTATCGTCTTTTGGAAAATAA

Upstream 100 bases:

>100_bases
TGGTATCCCAGCTGGTTCTGTAAATGCGTTTTTTGACGAACTTTATGATATGCCTGAATTAACACCAGTCGTAGCGAAGC
ATGAAGGGGCGGCATCCTAC

Downstream 100 bases:

>100_bases
GGTTGTTTGATTCACTAATAAAAAAATCATATAGGGATTTCCTATATGATTTTTTTATAAAGATTGATATGAATTGTGAA
TTACCTTAAGAATTGCTCCG

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MAAAYAKYTNQLSVCIGCSGPGATNLVTGAANAMREHLPVLFITGAVPLSTVGLNASQELDVEPVFRPVTKYSVTVNDVK
DVLKEVALAIEIAISGVPGPVHIAIPIDIQHENIENVEMPPLPKRLPIVPDLDKIKETARELVKREKGYIFTGQGIRNSV
QLLIEVAELLNWPIITTPQAKGYIPEEHPLLVGVFGFAGHEAASALINEGDGKALLIVGSSLGETATNNYNGNLIKDRFS
VQMDFDQSVFNRKYKVDIPVLGDINLSLLMLIEELKALGLQMDRAEFTVEKKKEEEEENIEEYNTKNVLLNLQKCLPSTT
RYTIDIGEFMSYVIHHMKVIDFDSYNINVHFGAMGSGIGSAIGSKLAEPERPVVCITGDGCFFMHGLEILTAKEYNLPIL
FVVMNNARLGMVYHGHSLQFRRTHPSFEQESINISAMAAAMNIPSYRVNELQDINQDVINNLMNVNGPAILEVALVDNNT
PPMGDRVKFLSSFGK

Sequences:

>Translated_495_residues
MAAAYAKYTNQLSVCIGCSGPGATNLVTGAANAMREHLPVLFITGAVPLSTVGLNASQELDVEPVFRPVTKYSVTVNDVK
DVLKEVALAIEIAISGVPGPVHIAIPIDIQHENIENVEMPPLPKRLPIVPDLDKIKETARELVKREKGYIFTGQGIRNSV
QLLIEVAELLNWPIITTPQAKGYIPEEHPLLVGVFGFAGHEAASALINEGDGKALLIVGSSLGETATNNYNGNLIKDRFS
VQMDFDQSVFNRKYKVDIPVLGDINLSLLMLIEELKALGLQMDRAEFTVEKKKEEEEENIEEYNTKNVLLNLQKCLPSTT
RYTIDIGEFMSYVIHHMKVIDFDSYNINVHFGAMGSGIGSAIGSKLAEPERPVVCITGDGCFFMHGLEILTAKEYNLPIL
FVVMNNARLGMVYHGHSLQFRRTHPSFEQESINISAMAAAMNIPSYRVNELQDINQDVINNLMNVNGPAILEVALVDNNT
PPMGDRVKFLSSFGK
>Mature_494_residues
AAAYAKYTNQLSVCIGCSGPGATNLVTGAANAMREHLPVLFITGAVPLSTVGLNASQELDVEPVFRPVTKYSVTVNDVKD
VLKEVALAIEIAISGVPGPVHIAIPIDIQHENIENVEMPPLPKRLPIVPDLDKIKETARELVKREKGYIFTGQGIRNSVQ
LLIEVAELLNWPIITTPQAKGYIPEEHPLLVGVFGFAGHEAASALINEGDGKALLIVGSSLGETATNNYNGNLIKDRFSV
QMDFDQSVFNRKYKVDIPVLGDINLSLLMLIEELKALGLQMDRAEFTVEKKKEEEEENIEEYNTKNVLLNLQKCLPSTTR
YTIDIGEFMSYVIHHMKVIDFDSYNINVHFGAMGSGIGSAIGSKLAEPERPVVCITGDGCFFMHGLEILTAKEYNLPILF
VVMNNARLGMVYHGHSLQFRRTHPSFEQESINISAMAAAMNIPSYRVNELQDINQDVINNLMNVNGPAILEVALVDNNTP
PMGDRVKFLSSFGK

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=458, Percent_Identity=25.1091703056769, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI93004078, Length=521, Percent_Identity=22.8406909788868, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1790104, Length=489, Percent_Identity=28.0163599182004, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI87081685, Length=491, Percent_Identity=27.6985743380855, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1786717, Length=469, Percent_Identity=27.7185501066098, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1787096, Length=476, Percent_Identity=23.9495798319328, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI1788716, Length=437, Percent_Identity=26.0869565217391, Blast_Score=112, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17542570, Length=465, Percent_Identity=26.2365591397849, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17531299, Length=415, Percent_Identity=24.0963855421687, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17531301, Length=423, Percent_Identity=24.113475177305, Blast_Score=104, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323755, Length=517, Percent_Identity=26.3056092843327, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6320816, Length=428, Percent_Identity=25.7009345794392, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI19922626, Length=527, Percent_Identity=23.1499051233397, Blast_Score=100, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 54417; Mature: 54286

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAAYAKYTNQLSVCIGCSGPGATNLVTGAANAMREHLPVLFITGAVPLSTVGLNASQEL
CCCCHHHHCCCEEEEEECCCCCCCCEEHHHHHHHHHCCCEEEEECCCCEEEECCCCCCCC
DVEPVFRPVTKYSVTVNDVKDVLKEVALAIEIAISGVPGPVHIAIPIDIQHENIENVEMP
CCCHHHCCCCCEEEEHHHHHHHHHHHHHEEEEEEECCCCCEEEEEEEEEECCCCCCCCCC
PLPKRLPIVPDLDKIKETARELVKREKGYIFTGQGIRNSVQLLIEVAELLNWPIITTPQA
CCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCEEECCCC
KGYIPEEHPLLVGVFGFAGHEAASALINEGDGKALLIVGSSLGETATNNYNGNLIKDRFS
CCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCEEECEEE
VQMDFDQSVFNRKYKVDIPVLGDINLSLLMLIEELKALGLQMDRAEFTVEKKKEEEEENI
EEECCCHHHHCCEEEEECEEECCCCHHHHHHHHHHHHHCCEECCHHHHHHHHHHHHHHHH
EEYNTKNVLLNLQKCLPSTTRYTIDIGEFMSYVIHHMKVIDFDSYNINVHFGAMGSGIGS
HHCCCHHHEEEHHHHCCCCCEEEEEHHHHHHHHHHHEEEEEECCEEEEEEECCCCCCHHH
AIGSKLAEPERPVVCITGDGCFFMHGLEILTAKEYNLPILFVVMNNARLGMVYHGHSLQF
HHHHHHCCCCCCEEEEECCCEEEEECEEEEEEECCCCCEEEEEECCCEEEEEEECCCEEE
RRTHPSFEQESINISAMAAAMNIPSYRVNELQDINQDVINNLMNVNGPAILEVALVDNNT
EECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCHHHHHHHHCCCCCEEEEEEEECCCC
PPMGDRVKFLSSFGK
CCCCHHHHHHHHHCC
>Mature Secondary Structure 
AAAYAKYTNQLSVCIGCSGPGATNLVTGAANAMREHLPVLFITGAVPLSTVGLNASQEL
CCCHHHHCCCEEEEEECCCCCCCCEEHHHHHHHHHCCCEEEEECCCCEEEECCCCCCCC
DVEPVFRPVTKYSVTVNDVKDVLKEVALAIEIAISGVPGPVHIAIPIDIQHENIENVEMP
CCCHHHCCCCCEEEEHHHHHHHHHHHHHEEEEEEECCCCCEEEEEEEEEECCCCCCCCCC
PLPKRLPIVPDLDKIKETARELVKREKGYIFTGQGIRNSVQLLIEVAELLNWPIITTPQA
CCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCEEECCCC
KGYIPEEHPLLVGVFGFAGHEAASALINEGDGKALLIVGSSLGETATNNYNGNLIKDRFS
CCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCEEECEEE
VQMDFDQSVFNRKYKVDIPVLGDINLSLLMLIEELKALGLQMDRAEFTVEKKKEEEEENI
EEECCCHHHHCCEEEEECEEECCCCHHHHHHHHHHHHHCCEECCHHHHHHHHHHHHHHHH
EEYNTKNVLLNLQKCLPSTTRYTIDIGEFMSYVIHHMKVIDFDSYNINVHFGAMGSGIGS
HHCCCHHHEEEHHHHCCCCCEEEEEHHHHHHHHHHHEEEEEECCEEEEEEECCCCCCHHH
AIGSKLAEPERPVVCITGDGCFFMHGLEILTAKEYNLPILFVVMNNARLGMVYHGHSLQF
HHHHHHCCCCCCEEEEECCCEEEEECEEEEEEECCCCCEEEEEECCCEEEEEEECCCEEE
RRTHPSFEQESINISAMAAAMNIPSYRVNELQDINQDVINNLMNVNGPAILEVALVDNNT
EECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCHHHHHHHHCCCCCEEEEEEEECCCC
PPMGDRVKFLSSFGK
CCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]