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The map label for this gene is atoC [C]

Identifier: 222094084

GI number: 222094084

Start: 355330

End: 356697

Strand: Direct

Name: atoC [C]

Synonym: BCQ_0377

Alternate gene names: 222094084

Gene position: 355330-356697 (Clockwise)

Preceding gene: 222094083

Following gene: 222094085

Centisome position: 6.81

GC content: 35.67

Gene sequence:

>1368_bases
ATGGTTGCAGAAAAGGAACGAGTGTTAATGGATTTACAAGATGTATTTGAATATGCGTTTGACGAAATTTTTGTGACTGA
CGAAAAGGGAATCGTTGTACGTGTAAATAGTACATGTGAAAGGCACTATCAATTGGCTGCAAAAGAGTTAGTAGGTAAAC
ATGTAAAAGAATTGCAGAAAGATGGTATCTTTTATCCATCAGCAACGTTAGAAGTAATTGAAAAAAAGAGGCCAATCGAA
CTCGTTCAGACGACAAAATCAGGAGAATATTTACACGTTCGTACAAGGCCTGTTTTTGATGAAGAGGGCAATTTAAGAAG
AGTAATTAGTTACTCTCGTGATCTTACAGAACTCTATCAATTACGTCAAAAGGTAGAAGAAATGGATAATCAGTTAAAAA
CATATAAAAAAGAATTAAGAGAAACATATGAACATGAAGGACTTATTTTTAAAAGTATCGCTATGCAAAAAATAGTCGAG
ACAATCAAGAAAGTATCCGTGGTAGATAGTACGGTTCTCGTTTTAGGTGAGACAGGAGTAGGAAAGAGTCGATTGGTGCG
TCATTTACATGAAGTGAGTAACCGGAAGAATGAAAGTTTCTATGAAATTAACTGCGCGGCATTGCCGACAAATTTAATTG
AATCAGAGCTTTTTGGATACTCAGGCGGATCCTTTACAGGTGCGAATCGTGAAGGGAAAAAGGGACTATTAGAATCAGCG
CATAAAGGAACACTATTCTTAGATGAAATTGGCGAAATGCCGCTTGAAACTCAAGCAAAGCTTTTGCAAGTGTTGCAAGA
AAAAACGTTTCGCCCTATAGGCGGAAGAGAATTAAAAAAGGTGGATGTTAGAATTGTAGCGGCAACAAATAGAGATTTAA
GTATGATGGTGAAACAAGGAACATTTCGGAAAGATTTATATTATCGTCTGAATGTCATTCCCATTGTAATCCCACCACTA
CGGGAAAGAACAGAAGACATTTTGCCACTTATTTATCATTATTTACAACACTTTAACGAGAAATATGGTAGAAATGTAAA
ATTAGCGCCTAGCACATTACAAATGTTTGTTGGTTACCCGTGGGAAGGAAATAATAGAGAGATTGAAAATGTGATTGAGC
GAATTGTTATAACTGCCGATGATATCGTTACGATAGAAGATTTACCAATAGCGATGCAAGAATCAACGGTTGAACAGTCC
GGTCAAAGTCTCTATAAAATGTTAGAAGAAGTAGAACGGAATATTATTTTGAAAGCATATAAAACATATGGATCGAGTTA
TAAAGTAGCGGAGTTTTTAAAAATAAGTCAATCTGCTGCCACTAGGAAAATAAAGAAACTTATAGAGGAGGAAGAGAATA
TTGGATAA

Upstream 100 bases:

>100_bases
CAGCAAACATTCTAGCTGACTCTATATAAAATGCAGAAAATTCACTTTATAATAAAGGTAGTGAAGTGAATCGACGGAGT
GGAAGTTAGGGGTGGCTAGG

Downstream 100 bases:

>100_bases
AAAAGCGACGTTTGTAAATGTAGAGAAAAAGGCGATGTATATAAATGGTGAGTGGGTTACATTGCAAGAGCAAATCGAAG
TAAATAATCCAGCAACGAAG

Product: sensory box sigma-54 dependent DNA-binding response regulator

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MVAEKERVLMDLQDVFEYAFDEIFVTDEKGIVVRVNSTCERHYQLAAKELVGKHVKELQKDGIFYPSATLEVIEKKRPIE
LVQTTKSGEYLHVRTRPVFDEEGNLRRVISYSRDLTELYQLRQKVEEMDNQLKTYKKELRETYEHEGLIFKSIAMQKIVE
TIKKVSVVDSTVLVLGETGVGKSRLVRHLHEVSNRKNESFYEINCAALPTNLIESELFGYSGGSFTGANREGKKGLLESA
HKGTLFLDEIGEMPLETQAKLLQVLQEKTFRPIGGRELKKVDVRIVAATNRDLSMMVKQGTFRKDLYYRLNVIPIVIPPL
RERTEDILPLIYHYLQHFNEKYGRNVKLAPSTLQMFVGYPWEGNNREIENVIERIVITADDIVTIEDLPIAMQESTVEQS
GQSLYKMLEEVERNIILKAYKTYGSSYKVAEFLKISQSAATRKIKKLIEEEENIG

Sequences:

>Translated_455_residues
MVAEKERVLMDLQDVFEYAFDEIFVTDEKGIVVRVNSTCERHYQLAAKELVGKHVKELQKDGIFYPSATLEVIEKKRPIE
LVQTTKSGEYLHVRTRPVFDEEGNLRRVISYSRDLTELYQLRQKVEEMDNQLKTYKKELRETYEHEGLIFKSIAMQKIVE
TIKKVSVVDSTVLVLGETGVGKSRLVRHLHEVSNRKNESFYEINCAALPTNLIESELFGYSGGSFTGANREGKKGLLESA
HKGTLFLDEIGEMPLETQAKLLQVLQEKTFRPIGGRELKKVDVRIVAATNRDLSMMVKQGTFRKDLYYRLNVIPIVIPPL
RERTEDILPLIYHYLQHFNEKYGRNVKLAPSTLQMFVGYPWEGNNREIENVIERIVITADDIVTIEDLPIAMQESTVEQS
GQSLYKMLEEVERNIILKAYKTYGSSYKVAEFLKISQSAATRKIKKLIEEEENIG
>Mature_455_residues
MVAEKERVLMDLQDVFEYAFDEIFVTDEKGIVVRVNSTCERHYQLAAKELVGKHVKELQKDGIFYPSATLEVIEKKRPIE
LVQTTKSGEYLHVRTRPVFDEEGNLRRVISYSRDLTELYQLRQKVEEMDNQLKTYKKELRETYEHEGLIFKSIAMQKIVE
TIKKVSVVDSTVLVLGETGVGKSRLVRHLHEVSNRKNESFYEINCAALPTNLIESELFGYSGGSFTGANREGKKGLLESA
HKGTLFLDEIGEMPLETQAKLLQVLQEKTFRPIGGRELKKVDVRIVAATNRDLSMMVKQGTFRKDLYYRLNVIPIVIPPL
RERTEDILPLIYHYLQHFNEKYGRNVKLAPSTLQMFVGYPWEGNNREIENVIERIVITADDIVTIEDLPIAMQESTVEQS
GQSLYKMLEEVERNIILKAYKTYGSSYKVAEFLKISQSAATRKIKKLIEEEENIG

Specific function: Member Of The Two-Component Regulatory System Atos/Atoc Involved In The Transcriptional Regulation Of The Ato Genes For Acetoacetate Metabolism. Also An Inhibitor Of Polyamine Biosynthesis. [C]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=351, Percent_Identity=41.3105413105413, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI1789233, Length=318, Percent_Identity=40.5660377358491, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI87082117, Length=231, Percent_Identity=48.0519480519481, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1789087, Length=231, Percent_Identity=49.3506493506493, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1790437, Length=334, Percent_Identity=37.7245508982036, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1790299, Length=328, Percent_Identity=39.6341463414634, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1788905, Length=263, Percent_Identity=41.06463878327, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1787583, Length=370, Percent_Identity=31.8918918918919, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1786524, Length=303, Percent_Identity=35.973597359736, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI87082152, Length=242, Percent_Identity=42.9752066115703, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87081872, Length=237, Percent_Identity=39.2405063291139, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI87081858, Length=463, Percent_Identity=26.7818574514039, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1789828, Length=243, Percent_Identity=36.2139917695473, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR002078 [H]

Pfam domain/function: PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52410; Mature: 52410

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAEKERVLMDLQDVFEYAFDEIFVTDEKGIVVRVNSTCERHYQLAAKELVGKHVKELQK
CCCCHHHHHHHHHHHHHHHHHHEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
DGIFYPSATLEVIEKKRPIELVQTTKSGEYLHVRTRPVFDEEGNLRRVISYSRDLTELYQ
CCCCCCCHHHHHHHCCCCHHHHEECCCCCEEEEECCCCCCCCCCHHHHHHHHCCHHHHHH
LRQKVEEMDNQLKTYKKELRETYEHEGLIFKSIAMQKIVETIKKVSVVDSTVLVLGETGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
GKSRLVRHLHEVSNRKNESFYEINCAALPTNLIESELFGYSGGSFTGANREGKKGLLESA
CHHHHHHHHHHHHCCCCCCEEEEEEHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHC
HKGTLFLDEIGEMPLETQAKLLQVLQEKTFRPIGGRELKKVDVRIVAATNRDLSMMVKQG
CCCCEEHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCHHHHHHCC
TFRKDLYYRLNVIPIVIPPLRERTEDILPLIYHYLQHFNEKYGRNVKLAPSTLQMFVGYP
CHHHHHHEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHEECC
WEGNNREIENVIERIVITADDIVTIEDLPIAMQESTVEQSGQSLYKMLEEVERNIILKAY
CCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTYGSSYKVAEFLKISQSAATRKIKKLIEEEENIG
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVAEKERVLMDLQDVFEYAFDEIFVTDEKGIVVRVNSTCERHYQLAAKELVGKHVKELQK
CCCCHHHHHHHHHHHHHHHHHHEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
DGIFYPSATLEVIEKKRPIELVQTTKSGEYLHVRTRPVFDEEGNLRRVISYSRDLTELYQ
CCCCCCCHHHHHHHCCCCHHHHEECCCCCEEEEECCCCCCCCCCHHHHHHHHCCHHHHHH
LRQKVEEMDNQLKTYKKELRETYEHEGLIFKSIAMQKIVETIKKVSVVDSTVLVLGETGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
GKSRLVRHLHEVSNRKNESFYEINCAALPTNLIESELFGYSGGSFTGANREGKKGLLESA
CHHHHHHHHHHHHCCCCCCEEEEEEHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHC
HKGTLFLDEIGEMPLETQAKLLQVLQEKTFRPIGGRELKKVDVRIVAATNRDLSMMVKQG
CCCCEEHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCHHHHHHCC
TFRKDLYYRLNVIPIVIPPLRERTEDILPLIYHYLQHFNEKYGRNVKLAPSTLQMFVGYP
CHHHHHHEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHEECC
WEGNNREIENVIERIVITADDIVTIEDLPIAMQESTVEQSGQSLYKMLEEVERNIILKAY
CCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTYGSSYKVAEFLKISQSAATRKIKKLIEEEENIG
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]