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The map label for this gene is ligA [H]

Identifier: 222094064

GI number: 222094064

Start: 333945

End: 335792

Strand: Direct

Name: ligA [H]

Synonym: BCQ_0357

Alternate gene names: 222094064

Gene position: 333945-335792 (Clockwise)

Preceding gene: 222094063

Following gene: 222094065

Centisome position: 6.4

GC content: 39.02

Gene sequence:

>1848_bases
ATGAGTGAAGATTCTCCCTCCGTTCGCGTTGGGGGAACGGTTCTTGATATATTTGAAAAAGTAACACATAAGTCACCGAT
GTTAAGTTTAGGAAATGCATTTAACGAAGGGGATTTACGTGATTTTGACCGACGAGTACGTCAAGGAATTGATGATGCGA
ATGTAAGATATATATGCGAATTAAAAATTGACGGACTTGCTGTTTCACTTCATTATGAAAAAGGACGCTTCATTCAAGGG
GCAACACGTGGTGATGGTGTAACAGGTGAAGATATTACCCAAAATTTAAAAACGATTAAAGCAATCCCGCTTCGTTTAAA
TGAAGAAGTAACGTTAGAGGCACGAGGCGAAGCTTATATGCCGAAGCGTTCATTCGTTAAGTTAAATGAAGAAAAAGAGC
AAAATGGTGAAGATGTATTTGCGAATCCGCGTAATGCGGCAGCAGGTTCAATCCGCCAACTTGATCCGAAAATTGCAGCG
AAGCGTAACTTATCTATGTTTGTATACGGTCTTGCGAATGTAGAAGAAAAAACAATCCTATCACATAGTGAATCGCTTGA
TTTCTTAGGTGAACTTGGATTCAAAACAAATCCAAATCGACGCACATGTGAAACAATTGAAGAAGTTATAGCTTATGTAG
AAGAATGGCAAGAAAAACGTCCGCATCTTGATTATGAGATTGATGGGATCGTTATAAAAGTAGATGATGTCGCTCTTCAA
GAAAGTCTAGGCACTACAGCAAAGAGTCCAAGATGGGCAATTGCTTATAAATTCCCAGCTGAAGAAGTTGTAACAAGATT
AACAGGCATTGAATTAAGTGTTGGTCGTACAGGGGTTGTAACACCGACTGCAGAGCTAGAGCCAGTGCGAGTGGCTGGTA
CGATCGTTCGTCGTGCTTCTTTACATAACGAAGATTTAATTCGTGAAAAAGATATTCGAATTGGTGACTACGTTGTTGTG
AAGAAGGCTGGAGATATTATTCCTGAAGTTGTGAATGTTCTTTTCGATAAGCGTACTGGTGAGGAAGAAGAATATCATAT
GCCAACGCATTGTCCAGCATGTGAGAGTGAACTAGTTCGTTTAGAAGAAGAGGTAGCACTTCGTTGTATAAATCCAACTT
GTCCTGCTCAAATTCGAGAAGGGTTAATCCATTTCGTTTCAAGAAATGCAATGAATATTGATGGACTTGGAGAACGTGTC
ATTACACAACTCTTTGAGGCAGATTATATTCGTACATTTGCTGATTTATATTCGTTGACGAAAGAGCAATTATTACAGCT
AGAACGATTCGGAGAAAAATCAGCAACGAATTTAGTACAAGCAATTGAGAATTCTAAAGAAAACTCATTAGAGCGATTAT
TATTCGGTCTTGGTATTCGCCATGTCGGAGCGAAAGCAGCACGTACATTTGCTGAGCATTTTGAAACGATGGATGCACTT
GTGAAAGCGACAGAAGAAGAACTAAAAGCAATTAACGAGATTGGTGAAAAAATGGCTCAATCCGTCGTGGCGTATTTTGA
TAATGAAGACGTATTAGAGTTATTACAACAATTTAAAGAATACGGCGTGAACATGACATATAAAGGCATGAAAATTGCTG
ATTTACAAAACGTTGAGTCGTACTTCGCAGGAAAAACTGTCGTTTTAACAGGGAAATTAGAAGTTATGGGACGTAGTGAA
GCGAAGAAGAAGATTGAGGCATTAGGTGGAAAAGTAACAGGAAGTGTTAGTAAAAGTACGGATTTAGTTGTTGCAGGTGA
AGCGGCAGGTTCGAAATTAGCACAAGCGGAGAAACATAATGTCGAGGTTTGGAATGAAGAGAGGTTCTTACAAGAGCTAA
ATAAGTAA

Upstream 100 bases:

>100_bases
CTATCAATATCACGTATTAGACAATCCTTCTGTTTCTGATGCGGAGTATGACCGTAATATGCAGGAGCTTATAAAATTAG
AAGCAGAGAATCCAGAGTTT

Downstream 100 bases:

>100_bases
GAGGTGCAAACTTACCATGAAAAAAATAGCATTAGCGGTATTAAGCCTTGGCCTACTTGTAAGTGGGTGTAGTGCAGGTG
CCGACAAAGATGAAAAAGTG

Product: nad-dependent DNA ligase liga

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 615; Mature: 614

Protein sequence:

>615_residues
MSEDSPSVRVGGTVLDIFEKVTHKSPMLSLGNAFNEGDLRDFDRRVRQGIDDANVRYICELKIDGLAVSLHYEKGRFIQG
ATRGDGVTGEDITQNLKTIKAIPLRLNEEVTLEARGEAYMPKRSFVKLNEEKEQNGEDVFANPRNAAAGSIRQLDPKIAA
KRNLSMFVYGLANVEEKTILSHSESLDFLGELGFKTNPNRRTCETIEEVIAYVEEWQEKRPHLDYEIDGIVIKVDDVALQ
ESLGTTAKSPRWAIAYKFPAEEVVTRLTGIELSVGRTGVVTPTAELEPVRVAGTIVRRASLHNEDLIREKDIRIGDYVVV
KKAGDIIPEVVNVLFDKRTGEEEEYHMPTHCPACESELVRLEEEVALRCINPTCPAQIREGLIHFVSRNAMNIDGLGERV
ITQLFEADYIRTFADLYSLTKEQLLQLERFGEKSATNLVQAIENSKENSLERLLFGLGIRHVGAKAARTFAEHFETMDAL
VKATEEELKAINEIGEKMAQSVVAYFDNEDVLELLQQFKEYGVNMTYKGMKIADLQNVESYFAGKTVVLTGKLEVMGRSE
AKKKIEALGGKVTGSVSKSTDLVVAGEAAGSKLAQAEKHNVEVWNEERFLQELNK

Sequences:

>Translated_615_residues
MSEDSPSVRVGGTVLDIFEKVTHKSPMLSLGNAFNEGDLRDFDRRVRQGIDDANVRYICELKIDGLAVSLHYEKGRFIQG
ATRGDGVTGEDITQNLKTIKAIPLRLNEEVTLEARGEAYMPKRSFVKLNEEKEQNGEDVFANPRNAAAGSIRQLDPKIAA
KRNLSMFVYGLANVEEKTILSHSESLDFLGELGFKTNPNRRTCETIEEVIAYVEEWQEKRPHLDYEIDGIVIKVDDVALQ
ESLGTTAKSPRWAIAYKFPAEEVVTRLTGIELSVGRTGVVTPTAELEPVRVAGTIVRRASLHNEDLIREKDIRIGDYVVV
KKAGDIIPEVVNVLFDKRTGEEEEYHMPTHCPACESELVRLEEEVALRCINPTCPAQIREGLIHFVSRNAMNIDGLGERV
ITQLFEADYIRTFADLYSLTKEQLLQLERFGEKSATNLVQAIENSKENSLERLLFGLGIRHVGAKAARTFAEHFETMDAL
VKATEEELKAINEIGEKMAQSVVAYFDNEDVLELLQQFKEYGVNMTYKGMKIADLQNVESYFAGKTVVLTGKLEVMGRSE
AKKKIEALGGKVTGSVSKSTDLVVAGEAAGSKLAQAEKHNVEVWNEERFLQELNK
>Mature_614_residues
SEDSPSVRVGGTVLDIFEKVTHKSPMLSLGNAFNEGDLRDFDRRVRQGIDDANVRYICELKIDGLAVSLHYEKGRFIQGA
TRGDGVTGEDITQNLKTIKAIPLRLNEEVTLEARGEAYMPKRSFVKLNEEKEQNGEDVFANPRNAAAGSIRQLDPKIAAK
RNLSMFVYGLANVEEKTILSHSESLDFLGELGFKTNPNRRTCETIEEVIAYVEEWQEKRPHLDYEIDGIVIKVDDVALQE
SLGTTAKSPRWAIAYKFPAEEVVTRLTGIELSVGRTGVVTPTAELEPVRVAGTIVRRASLHNEDLIREKDIRIGDYVVVK
KAGDIIPEVVNVLFDKRTGEEEEYHMPTHCPACESELVRLEEEVALRCINPTCPAQIREGLIHFVSRNAMNIDGLGERVI
TQLFEADYIRTFADLYSLTKEQLLQLERFGEKSATNLVQAIENSKENSLERLLFGLGIRHVGAKAARTFAEHFETMDALV
KATEEELKAINEIGEKMAQSVVAYFDNEDVLELLQQFKEYGVNMTYKGMKIADLQNVESYFAGKTVVLTGKLEVMGRSEA
KKKIEALGGKVTGSVSKSTDLVVAGEAAGSKLAQAEKHNVEVWNEERFLQELNK

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=619, Percent_Identity=47.0113085621971, Blast_Score=562, Evalue=1e-161,
Organism=Escherichia coli, GI87082305, Length=460, Percent_Identity=23.9130434782609, Blast_Score=106, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 68730; Mature: 68599

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDSPSVRVGGTVLDIFEKVTHKSPMLSLGNAFNEGDLRDFDRRVRQGIDDANVRYICE
CCCCCCCEEECHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEE
LKIDGLAVSLHYEKGRFIQGATRGDGVTGEDITQNLKTIKAIPLRLNEEVTLEARGEAYM
EEECCEEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHCCEECCCEEEEEECCCCCC
PKRSFVKLNEEKEQNGEDVFANPRNAAAGSIRQLDPKIAAKRNLSMFVYGLANVEEKTIL
CCHHHEECCCHHCCCCCCCCCCCCCCCCCCHHHCCHHHHHHCCCEEEEEEECCCCHHHHH
SHSESLDFLGELGFKTNPNRRTCETIEEVIAYVEEWQEKRPHLDYEIDGIVIKVDDVALQ
HCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECEEEEEECCHHHH
ESLGTTAKSPRWAIAYKFPAEEVVTRLTGIELSVGRTGVVTPTAELEPVRVAGTIVRRAS
HHCCCCCCCCCEEEEEECCHHHHHHHHHCCEEECCCCCEECCCCCCCCHHHHHHHHHHHH
LHNEDLIREKDIRIGDYVVVKKAGDIIPEVVNVLFDKRTGEEEEYHMPTHCPACESELVR
CCCHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
LEEEVALRCINPTCPAQIREGLIHFVSRNAMNIDGLGERVITQLFEADYIRTFADLYSLT
HHHHHHHEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KEQLLQLERFGEKSATNLVQAIENSKENSLERLLFGLGIRHVGAKAARTFAEHFETMDAL
HHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKATEEELKAINEIGEKMAQSVVAYFDNEDVLELLQQFKEYGVNMTYKGMKIADLQNVES
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCEEEHHHHHHHH
YFAGKTVVLTGKLEVMGRSEAKKKIEALGGKVTGSVSKSTDLVVAGEAAGSKLAQAEKHN
HHCCCEEEEEEEEEECCCHHHHHHHHHHCCCEECCCCCCCCEEEECCCCCHHHHHHHHCC
VEVWNEERFLQELNK
CEEECHHHHHHHHCC
>Mature Secondary Structure 
SEDSPSVRVGGTVLDIFEKVTHKSPMLSLGNAFNEGDLRDFDRRVRQGIDDANVRYICE
CCCCCCEEECHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEE
LKIDGLAVSLHYEKGRFIQGATRGDGVTGEDITQNLKTIKAIPLRLNEEVTLEARGEAYM
EEECCEEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHCCEECCCEEEEEECCCCCC
PKRSFVKLNEEKEQNGEDVFANPRNAAAGSIRQLDPKIAAKRNLSMFVYGLANVEEKTIL
CCHHHEECCCHHCCCCCCCCCCCCCCCCCCHHHCCHHHHHHCCCEEEEEEECCCCHHHHH
SHSESLDFLGELGFKTNPNRRTCETIEEVIAYVEEWQEKRPHLDYEIDGIVIKVDDVALQ
HCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECEEEEEECCHHHH
ESLGTTAKSPRWAIAYKFPAEEVVTRLTGIELSVGRTGVVTPTAELEPVRVAGTIVRRAS
HHCCCCCCCCCEEEEEECCHHHHHHHHHCCEEECCCCCEECCCCCCCCHHHHHHHHHHHH
LHNEDLIREKDIRIGDYVVVKKAGDIIPEVVNVLFDKRTGEEEEYHMPTHCPACESELVR
CCCHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
LEEEVALRCINPTCPAQIREGLIHFVSRNAMNIDGLGERVITQLFEADYIRTFADLYSLT
HHHHHHHEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KEQLLQLERFGEKSATNLVQAIENSKENSLERLLFGLGIRHVGAKAARTFAEHFETMDAL
HHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKATEEELKAINEIGEKMAQSVVAYFDNEDVLELLQQFKEYGVNMTYKGMKIADLQNVES
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCEEEHHHHHHHH
YFAGKTVVLTGKLEVMGRSEAKKKIEALGGKVTGSVSKSTDLVVAGEAAGSKLAQAEKHN
HHCCCEEEEEEEEEECCCHHHHHHHHHHCCCEECCCCCCCCEEEECCCCCHHHHHHHHCC
VEVWNEERFLQELNK
CEEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA