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The map label for this gene is pcrA [H]

Identifier: 222094063

GI number: 222094063

Start: 331524

End: 333767

Strand: Direct

Name: pcrA [H]

Synonym: BCQ_0356

Alternate gene names: 222094063

Gene position: 331524-333767 (Clockwise)

Preceding gene: 222094062

Following gene: 222094064

Centisome position: 6.36

GC content: 37.92

Gene sequence:

>2244_bases
ATGAGTATGACAGATAGATTATTAAATGGTTTAAATCCGCAGCAACAAAAAGCAGTACAAACAACGAACGGACCACTTCT
ATTAATGGCAGGCGCAGGTAGTGGTAAAACGCGTGTGTTAACACATCGTATTGCGTATTTACTCGGTGAAAAAGGAGTAG
CGCCATGGAATGTACTAGCTATTACCTTTACAAATAAAGCCGCTCGTGAAATGCGCGAGCGTATTGATACACTTGTCGGA
CCAGAAGCTGAAGATATTTGGATTTCTACGTTCCACTCTATGTGTGTACGTATTTTACGACGTGATATCGATCGCATTGG
TATTAATCGTAATTTTACAATTTTAGATTCAGGTGATCAGTTAACTGTAGTCAAAAAAATTATGAAAGAGCGCAATATTG
ATCCGAAGAAATTTGAACCGCGCTCTATTTTAGCTGGTATTAGTAATGCGAAAAATGAATTGTTATCTGCGGATAAATAT
GCAAAGAAAATTACAATTGCTGATCCATATGAAAAATTAACGAGCGATGTATATACAGAATATCAAAAACGTCTTTTGAA
AAATAACTCACTAGACTTTGATGATTTAATTATGACGACGATTCAACTATTTGAACGTGTTCCAGAAGTACTAGAGTTTT
ATCAGCGCAAATTCCAGTATATTCACGTGGATGAGTATCAAGATACGAACAAAGCGCAGTACCTTCTTGTTAAACATTTA
GCAGCACGTTTTAAAAATTTATGTGTTGTAGGTGACTCAGATCAGTCAATTTATCGCTGGCGTGGTGCGGACATTTCCAA
CATTTTGTCATTCGAAAAAGACTATGAGAATGCGCAAGTTATCCTGTTAGAACAAAATTATCGTTCGTCACAAAATATTT
TAAATGCAGCGAATGCGGTTATTGAAAGAAATACGAATCGTAAACCGAAGAAATTATGGACAGATAATGAAGTTGGAAAC
AAAATTTCGTATTATCGTGCTGCAACCGAAAAGGACGAAGCATACTTTGTTGCGAAAAAAATTCGCGACGATATTCAAAT
GGGAAAACGAAAATATACTGATTTTGCAGTGTTATATCGTACAAATGCGCAGTCTCGTATGGTCGAGGAGATTTTCCTGA
AATCTAATATTCCTTACAAAATTGTCGGCGGTACGAAGTTCTACGACCGTAAAGAGATTAAAGACATTTTGGCGTATTTA
CGTTTAATTGGGAATCCAGATGATGAGATTAGTTTTGCGCGTATTATTAACGTACCAAAACGCGGAATTGGTGCGACTTC
TATCGACAAAATTATTAATTATGGTGTACAAAATGGAATTTCTTTAACTGCTGTATTCGATGAGATTGAGCATGTTGGAG
TAAGTGCAAAGGTTACAAAGGCAGTAAAGGAATTCGCAGGTTTATTACACAACTGGGTAAATATGCAAGAGTATTTATCT
GTTACAGAATTGGTAGAAGAAGTTATTGAGAAAACAGGCTATCGCGATATGTTGAAAAATGAGCGTACGTTAGAAGCAGA
AGGTCGTCTAGAGAACTTAGATGAGTTTTTATCTGTTACGCAAACATTTGAATCTCAAAGTGAAGATAAGAGTCTTGTTG
CATTCTTAACAGACTTAGCACTTGTTGCAGATATTGATCGTGTAGATGAAGACCCAACTGCTGGTGAGGAAGTTATTTTA
ATGACGATGCACTCAGCGAAAGGATTAGAATTCCCAGTTGTCTTTATTGTTGGTCTAGAGGAAGGAATATTCCCGCATAC
TCGTTCTCTAATGGAAGAAGATGAAATGCAAGAAGAGCGTCGTCTTGCTTATGTAGGTATTACTCGTGCAGAAGAAGAGT
TGTATTTATCTAATGCACAAATGCGTACTTTATTTGGTAGAACAAGTATGAATGCCGCATCTCGATTTATTACAGAAATC
CCGACAGAACTAGTGGAATCATTAAATGAAACAGCGCCGAAGCGTGAAACTTCATTTGGTGCAAAAGGAAGAACGGCAAG
TAGCAGTAAAACGACAACTACAACACGTTCTCGCTCAGCTTTCGCGCGTCCTGCAGCTAAGACGACAGGCGGCGAACAAA
TTGGCTGGGCAGTAGGCGATAAAGCTTCCCACCAAAAATGGGGAGTCGGTACAGTTGTAAGTGTAAAAGGTGAAGGAGAT
GCAAAAGAATTAGATATTGCGTTCCCAAGCCCAATTGGTGTTAAACGTTTGTTAGCAAAATTTGCACCTGTGACGAAACA
ATAG

Upstream 100 bases:

>100_bases
GCATGCTGATACAGTAGTTGTCGGAAATATTATTTATGATGATATAAAAGCAGCGTTAAAAACGGTTAAAGCAGTAAAAG
GAGAGTAGGTGCAGGCGTAT

Downstream 100 bases:

>100_bases
GAAAGGAATGAGGATATGTCAAAAGAGATAGCAAAAAAACGTATAGAAGAATTACGTGATTTGTTAAATACATTTAACTA
TCAATATCACGTATTAGACA

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 747; Mature: 746

Protein sequence:

>747_residues
MSMTDRLLNGLNPQQQKAVQTTNGPLLLMAGAGSGKTRVLTHRIAYLLGEKGVAPWNVLAITFTNKAAREMRERIDTLVG
PEAEDIWISTFHSMCVRILRRDIDRIGINRNFTILDSGDQLTVVKKIMKERNIDPKKFEPRSILAGISNAKNELLSADKY
AKKITIADPYEKLTSDVYTEYQKRLLKNNSLDFDDLIMTTIQLFERVPEVLEFYQRKFQYIHVDEYQDTNKAQYLLVKHL
AARFKNLCVVGDSDQSIYRWRGADISNILSFEKDYENAQVILLEQNYRSSQNILNAANAVIERNTNRKPKKLWTDNEVGN
KISYYRAATEKDEAYFVAKKIRDDIQMGKRKYTDFAVLYRTNAQSRMVEEIFLKSNIPYKIVGGTKFYDRKEIKDILAYL
RLIGNPDDEISFARIINVPKRGIGATSIDKIINYGVQNGISLTAVFDEIEHVGVSAKVTKAVKEFAGLLHNWVNMQEYLS
VTELVEEVIEKTGYRDMLKNERTLEAEGRLENLDEFLSVTQTFESQSEDKSLVAFLTDLALVADIDRVDEDPTAGEEVIL
MTMHSAKGLEFPVVFIVGLEEGIFPHTRSLMEEDEMQEERRLAYVGITRAEEELYLSNAQMRTLFGRTSMNAASRFITEI
PTELVESLNETAPKRETSFGAKGRTASSSKTTTTTRSRSAFARPAAKTTGGEQIGWAVGDKASHQKWGVGTVVSVKGEGD
AKELDIAFPSPIGVKRLLAKFAPVTKQ

Sequences:

>Translated_747_residues
MSMTDRLLNGLNPQQQKAVQTTNGPLLLMAGAGSGKTRVLTHRIAYLLGEKGVAPWNVLAITFTNKAAREMRERIDTLVG
PEAEDIWISTFHSMCVRILRRDIDRIGINRNFTILDSGDQLTVVKKIMKERNIDPKKFEPRSILAGISNAKNELLSADKY
AKKITIADPYEKLTSDVYTEYQKRLLKNNSLDFDDLIMTTIQLFERVPEVLEFYQRKFQYIHVDEYQDTNKAQYLLVKHL
AARFKNLCVVGDSDQSIYRWRGADISNILSFEKDYENAQVILLEQNYRSSQNILNAANAVIERNTNRKPKKLWTDNEVGN
KISYYRAATEKDEAYFVAKKIRDDIQMGKRKYTDFAVLYRTNAQSRMVEEIFLKSNIPYKIVGGTKFYDRKEIKDILAYL
RLIGNPDDEISFARIINVPKRGIGATSIDKIINYGVQNGISLTAVFDEIEHVGVSAKVTKAVKEFAGLLHNWVNMQEYLS
VTELVEEVIEKTGYRDMLKNERTLEAEGRLENLDEFLSVTQTFESQSEDKSLVAFLTDLALVADIDRVDEDPTAGEEVIL
MTMHSAKGLEFPVVFIVGLEEGIFPHTRSLMEEDEMQEERRLAYVGITRAEEELYLSNAQMRTLFGRTSMNAASRFITEI
PTELVESLNETAPKRETSFGAKGRTASSSKTTTTTRSRSAFARPAAKTTGGEQIGWAVGDKASHQKWGVGTVVSVKGEGD
AKELDIAFPSPIGVKRLLAKFAPVTKQ
>Mature_746_residues
SMTDRLLNGLNPQQQKAVQTTNGPLLLMAGAGSGKTRVLTHRIAYLLGEKGVAPWNVLAITFTNKAAREMRERIDTLVGP
EAEDIWISTFHSMCVRILRRDIDRIGINRNFTILDSGDQLTVVKKIMKERNIDPKKFEPRSILAGISNAKNELLSADKYA
KKITIADPYEKLTSDVYTEYQKRLLKNNSLDFDDLIMTTIQLFERVPEVLEFYQRKFQYIHVDEYQDTNKAQYLLVKHLA
ARFKNLCVVGDSDQSIYRWRGADISNILSFEKDYENAQVILLEQNYRSSQNILNAANAVIERNTNRKPKKLWTDNEVGNK
ISYYRAATEKDEAYFVAKKIRDDIQMGKRKYTDFAVLYRTNAQSRMVEEIFLKSNIPYKIVGGTKFYDRKEIKDILAYLR
LIGNPDDEISFARIINVPKRGIGATSIDKIINYGVQNGISLTAVFDEIEHVGVSAKVTKAVKEFAGLLHNWVNMQEYLSV
TELVEEVIEKTGYRDMLKNERTLEAEGRLENLDEFLSVTQTFESQSEDKSLVAFLTDLALVADIDRVDEDPTAGEEVILM
TMHSAKGLEFPVVFIVGLEEGIFPHTRSLMEEDEMQEERRLAYVGITRAEEELYLSNAQMRTLFGRTSMNAASRFITEIP
TELVESLNETAPKRETSFGAKGRTASSSKTTTTTRSRSAFARPAAKTTGGEQIGWAVGDKASHQKWGVGTVVSVKGEGDA
KELDIAFPSPIGVKRLLAKFAPVTKQ

Specific function: DNA helicase used for plasmid rolling-circle replication and also involved in UV repair [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=745, Percent_Identity=39.3288590604027, Blast_Score=529, Evalue=1e-151,
Organism=Escherichia coli, GI48994965, Length=643, Percent_Identity=39.6578538102644, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1787196, Length=347, Percent_Identity=27.9538904899135, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6322369, Length=772, Percent_Identity=29.1450777202073, Blast_Score=246, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6324477, Length=688, Percent_Identity=23.1104651162791, Blast_Score=107, Evalue=7e-24,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 84505; Mature: 84374

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMTDRLLNGLNPQQQKAVQTTNGPLLLMAGAGSGKTRVLTHRIAYLLGEKGVAPWNVLA
CCHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
ITFTNKAAREMRERIDTLVGPEAEDIWISTFHSMCVRILRRDIDRIGINRNFTILDSGDQ
EEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
LTVVKKIMKERNIDPKKFEPRSILAGISNAKNELLSADKYAKKITIADPYEKLTSDVYTE
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHH
YQKRLLKNNSLDFDDLIMTTIQLFERVPEVLEFYQRKFQYIHVDEYQDTNKAQYLLVKHL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHH
AARFKNLCVVGDSDQSIYRWRGADISNILSFEKDYENAQVILLEQNYRSSQNILNAANAV
HHHHHCEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
IERNTNRKPKKLWTDNEVGNKISYYRAATEKDEAYFVAKKIRDDIQMGKRKYTDFAVLYR
HHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEE
TNAQSRMVEEIFLKSNIPYKIVGGTKFYDRKEIKDILAYLRLIGNPDDEISFARIINVPK
CCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
RGIGATSIDKIINYGVQNGISLTAVFDEIEHVGVSAKVTKAVKEFAGLLHNWVNMQEYLS
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHH
VTELVEEVIEKTGYRDMLKNERTLEAEGRLENLDEFLSVTQTFESQSEDKSLVAFLTDLA
HHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LVADIDRVDEDPTAGEEVILMTMHSAKGLEFPVVFIVGLEEGIFPHTRSLMEEDEMQEER
HHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
RLAYVGITRAEEELYLSNAQMRTLFGRTSMNAASRFITEIPTELVESLNETAPKRETSFG
CEEEEEEECCHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
AKGRTASSSKTTTTTRSRSAFARPAAKTTGGEQIGWAVGDKASHQKWGVGTVVSVKGEGD
CCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEECEECCCCCCCCCCCCCEEEEEECCCC
AKELDIAFPSPIGVKRLLAKFAPVTKQ
CCEEEEECCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
SMTDRLLNGLNPQQQKAVQTTNGPLLLMAGAGSGKTRVLTHRIAYLLGEKGVAPWNVLA
CHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
ITFTNKAAREMRERIDTLVGPEAEDIWISTFHSMCVRILRRDIDRIGINRNFTILDSGDQ
EEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
LTVVKKIMKERNIDPKKFEPRSILAGISNAKNELLSADKYAKKITIADPYEKLTSDVYTE
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHH
YQKRLLKNNSLDFDDLIMTTIQLFERVPEVLEFYQRKFQYIHVDEYQDTNKAQYLLVKHL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHH
AARFKNLCVVGDSDQSIYRWRGADISNILSFEKDYENAQVILLEQNYRSSQNILNAANAV
HHHHHCEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
IERNTNRKPKKLWTDNEVGNKISYYRAATEKDEAYFVAKKIRDDIQMGKRKYTDFAVLYR
HHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEE
TNAQSRMVEEIFLKSNIPYKIVGGTKFYDRKEIKDILAYLRLIGNPDDEISFARIINVPK
CCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
RGIGATSIDKIINYGVQNGISLTAVFDEIEHVGVSAKVTKAVKEFAGLLHNWVNMQEYLS
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHH
VTELVEEVIEKTGYRDMLKNERTLEAEGRLENLDEFLSVTQTFESQSEDKSLVAFLTDLA
HHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LVADIDRVDEDPTAGEEVILMTMHSAKGLEFPVVFIVGLEEGIFPHTRSLMEEDEMQEER
HHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
RLAYVGITRAEEELYLSNAQMRTLFGRTSMNAASRFITEIPTELVESLNETAPKRETSFG
CEEEEEEECCHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
AKGRTASSSKTTTTTRSRSAFARPAAKTTGGEQIGWAVGDKASHQKWGVGTVVSVKGEGD
CCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEECEECCCCCCCCCCCCCEEEEEECCCC
AKELDIAFPSPIGVKRLLAKFAPVTKQ
CCEEEEECCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377 [H]