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The map label for this gene is purH [H]

Identifier: 222094057

GI number: 222094057

Start: 325892

End: 327427

Strand: Direct

Name: purH [H]

Synonym: BCQ_0350

Alternate gene names: 222094057

Gene position: 325892-327427 (Clockwise)

Preceding gene: 222094056

Following gene: 222094058

Centisome position: 6.25

GC content: 37.57

Gene sequence:

>1536_bases
ATGAAAAAGCGTGCATTAGTAAGTGTTTCAGATAAAACAGGAGTAGTAGAATTTGTTAAAGGTTTACTAGAACAAGGGAT
CGAAGTTATATCAACAGGTGGTACGAAAAAATTACTAGAAGAAAACGGCTTACAAGTAATCGGTATTTCTGAAGTAACTG
GTTTCCCAGAAATTATGGATGGTCGTGTGAAAACATTACATCCAAATATTCATGGTGGTCTATTAGCAGTTCGTGATAAT
GAAACACATGTAGCGCAAATGAATGAATTAGGTATGGAGCCGATTGATTTTGTTGTCGTTAACTTATACCCATTCAAAGA
AACAATCGCTAAGCCTGATGTAACATTTGCTGATGCAATTGAAAATATTGATATCGGTGGCCCGACAATGATTCGCTCTG
CTGCGAAAAATCATAAATTCGTGTCTGTAATTGTAGATCCAGTAGATTACGATATTGTATTAGCAGAACTGAAAGAAAAC
GGTGAAGTAAAAGAAGAAACGAAACGTAAATTAGCAGCGAAAGTATTTCGTCATACAGCGGCATATGATGCGTTAATCTC
TAACTACTTAACAGAGCAAATGGGCGAAGAAAGCCCAGAAACATTAACAGTGACATTTGAGAAAAAACAAGATTTACGCT
ATGGAGAAAATCCACATCAAAAAGCAACATTTTATAAAGCACCATTCGCTGTAACTTCTTCTGTTGCGTACGCAGAACAA
TTACATGGCAAAGAATTATCGTATAACAATATTAATGATGCAGACGCAGCGCTTAGCATCGTGAAAGAATTTACAGAACC
AGCAGTAGTAGCGGTAAAACATATGAATCCATGTGGTGTTGGAGTAGGTACGGATATTCATGAAGCATATACACGTGCTT
ATGAAGCGGATCCAGTATCCATTTTTGGAGGCATTATTGCAGCGAATCGTGAAATTGATAAAGCTACAGCTGAAAAGTTA
CACGAAATTTTCTTAGAAATTATTATTGCACCTTCTTTCTCAAAAGAAGCTTTAGAAGTATTGCAAAGTAAGAAAAACTT
ACGTTTACTAACTGTAAATATTGAAAAAGCGACAAGTGCAAGTAAAAAACTAACTTCTGTACAAGGTGGCCTTCTCGTTC
AAGAGGAAGATACGTTATCGTTAGATGAGAGCACAATTTCAATTCCAACAAAACGTGAACCGTCAGAGCAAGAATGGAAA
GATTTAAAACTAGCTTGGAAAGTTGTAAAGCATGTAAAATCAAATGCAATTGTTTTAGCGAAAGATGATATGACAATTGG
TGTCGGTGCGGGACAGATGAACCGTGTAGGTTCTGCAAAAATCGCAATTACACAGGCTGGTGAAAAAGCACAAGGTAGCG
CGCTTGCATCTGATGCTTTCTTCCCAATGCCAGATACATTAGAAGAAGCAGCAAAAGCAGGAATTACAGCAATCATTCAA
CCGGGCGGATCAATCCGTGATGAAGATTCTATTAAAGTGGCGGATACGTATGGGATTGCTATGGTGTTCACTGGCGTACG
TCATTTCAAACACTAA

Upstream 100 bases:

>100_bases
ACAAGTTGAACATAAATTATACGTAAATACAGTGAATCAAATTGTTCAATCTGTGAAAGAACCAACTGTTAACTAACATA
CAACCAGGGGTGAAGGATAA

Downstream 100 bases:

>100_bases
TAATAGAGGAAGTTTAAAAAGTCCGGTTAAGATAACCTTCCTTTTTAAACTTTAATTGATATGAAAAAACAAACTAGGGG
CATAGCTTCTAGTTTGTACT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/imp cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MKKRALVSVSDKTGVVEFVKGLLEQGIEVISTGGTKKLLEENGLQVIGISEVTGFPEIMDGRVKTLHPNIHGGLLAVRDN
ETHVAQMNELGMEPIDFVVVNLYPFKETIAKPDVTFADAIENIDIGGPTMIRSAAKNHKFVSVIVDPVDYDIVLAELKEN
GEVKEETKRKLAAKVFRHTAAYDALISNYLTEQMGEESPETLTVTFEKKQDLRYGENPHQKATFYKAPFAVTSSVAYAEQ
LHGKELSYNNINDADAALSIVKEFTEPAVVAVKHMNPCGVGVGTDIHEAYTRAYEADPVSIFGGIIAANREIDKATAEKL
HEIFLEIIIAPSFSKEALEVLQSKKNLRLLTVNIEKATSASKKLTSVQGGLLVQEEDTLSLDESTISIPTKREPSEQEWK
DLKLAWKVVKHVKSNAIVLAKDDMTIGVGAGQMNRVGSAKIAITQAGEKAQGSALASDAFFPMPDTLEEAAKAGITAIIQ
PGGSIRDEDSIKVADTYGIAMVFTGVRHFKH

Sequences:

>Translated_511_residues
MKKRALVSVSDKTGVVEFVKGLLEQGIEVISTGGTKKLLEENGLQVIGISEVTGFPEIMDGRVKTLHPNIHGGLLAVRDN
ETHVAQMNELGMEPIDFVVVNLYPFKETIAKPDVTFADAIENIDIGGPTMIRSAAKNHKFVSVIVDPVDYDIVLAELKEN
GEVKEETKRKLAAKVFRHTAAYDALISNYLTEQMGEESPETLTVTFEKKQDLRYGENPHQKATFYKAPFAVTSSVAYAEQ
LHGKELSYNNINDADAALSIVKEFTEPAVVAVKHMNPCGVGVGTDIHEAYTRAYEADPVSIFGGIIAANREIDKATAEKL
HEIFLEIIIAPSFSKEALEVLQSKKNLRLLTVNIEKATSASKKLTSVQGGLLVQEEDTLSLDESTISIPTKREPSEQEWK
DLKLAWKVVKHVKSNAIVLAKDDMTIGVGAGQMNRVGSAKIAITQAGEKAQGSALASDAFFPMPDTLEEAAKAGITAIIQ
PGGSIRDEDSIKVADTYGIAMVFTGVRHFKH
>Mature_511_residues
MKKRALVSVSDKTGVVEFVKGLLEQGIEVISTGGTKKLLEENGLQVIGISEVTGFPEIMDGRVKTLHPNIHGGLLAVRDN
ETHVAQMNELGMEPIDFVVVNLYPFKETIAKPDVTFADAIENIDIGGPTMIRSAAKNHKFVSVIVDPVDYDIVLAELKEN
GEVKEETKRKLAAKVFRHTAAYDALISNYLTEQMGEESPETLTVTFEKKQDLRYGENPHQKATFYKAPFAVTSSVAYAEQ
LHGKELSYNNINDADAALSIVKEFTEPAVVAVKHMNPCGVGVGTDIHEAYTRAYEADPVSIFGGIIAANREIDKATAEKL
HEIFLEIIIAPSFSKEALEVLQSKKNLRLLTVNIEKATSASKKLTSVQGGLLVQEEDTLSLDESTISIPTKREPSEQEWK
DLKLAWKVVKHVKSNAIVLAKDDMTIGVGAGQMNRVGSAKIAITQAGEKAQGSALASDAFFPMPDTLEEAAKAGITAIIQ
PGGSIRDEDSIKVADTYGIAMVFTGVRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=481, Percent_Identity=37.4220374220374, Blast_Score=275, Evalue=9e-74,
Organism=Escherichia coli, GI1790439, Length=524, Percent_Identity=53.0534351145038, Blast_Score=548, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI71985564, Length=484, Percent_Identity=34.0909090909091, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI71985574, Length=312, Percent_Identity=26.9230769230769, Blast_Score=92, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6323768, Length=478, Percent_Identity=35.1464435146443, Blast_Score=280, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6323056, Length=470, Percent_Identity=35.7446808510638, Blast_Score=280, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24649832, Length=481, Percent_Identity=38.8773388773389, Blast_Score=294, Evalue=8e-80,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55630; Mature: 55630

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRALVSVSDKTGVVEFVKGLLEQGIEVISTGGTKKLLEENGLQVIGISEVTGFPEIMD
CCCCEEEEECCCCCHHHHHHHHHHCCHHEEECCCHHHHHHHCCCEEEEEHHHCCCHHHHC
GRVKTLHPNIHGGLLAVRDNETHVAQMNELGMEPIDFVVVNLYPFKETIAKPDVTFADAI
CCEEEECCCCCCCEEEEECCCHHHHHHHHCCCCCEEEEEEEEEECHHHHCCCCCHHHHHH
ENIDIGGPTMIRSAAKNHKFVSVIVDPVDYDIVLAELKENGEVKEETKRKLAAKVFRHTA
HCCCCCCHHHHHHHHCCCCEEEEEECCCCCEEEEEEHHCCCCHHHHHHHHHHHHHHHHHH
AYDALISNYLTEQMGEESPETLTVTFEKKQDLRYGENPHQKATFYKAPFAVTSSVAYAEQ
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHH
LHGKELSYNNINDADAALSIVKEFTEPAVVAVKHMNPCGVGVGTDIHEAYTRAYEADPVS
HCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCHH
IFGGIIAANREIDKATAEKLHEIFLEIIIAPSFSKEALEVLQSKKNLRLLTVNIEKATSA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEEEHHHHCH
SKKLTSVQGGLLVQEEDTLSLDESTISIPTKREPSEQEWKDLKLAWKVVKHVKSNAIVLA
HHHHHHHCCCEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
KDDMTIGVGAGQMNRVGSAKIAITQAGEKAQGSALASDAFFPMPDTLEEAAKAGITAIIQ
ECCEEEEECCCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCCHHHHHHHHCCCEEEEC
PGGSIRDEDSIKVADTYGIAMVFTGVRHFKH
CCCCCCCCCCEEEEHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKRALVSVSDKTGVVEFVKGLLEQGIEVISTGGTKKLLEENGLQVIGISEVTGFPEIMD
CCCCEEEEECCCCCHHHHHHHHHHCCHHEEECCCHHHHHHHCCCEEEEEHHHCCCHHHHC
GRVKTLHPNIHGGLLAVRDNETHVAQMNELGMEPIDFVVVNLYPFKETIAKPDVTFADAI
CCEEEECCCCCCCEEEEECCCHHHHHHHHCCCCCEEEEEEEEEECHHHHCCCCCHHHHHH
ENIDIGGPTMIRSAAKNHKFVSVIVDPVDYDIVLAELKENGEVKEETKRKLAAKVFRHTA
HCCCCCCHHHHHHHHCCCCEEEEEECCCCCEEEEEEHHCCCCHHHHHHHHHHHHHHHHHH
AYDALISNYLTEQMGEESPETLTVTFEKKQDLRYGENPHQKATFYKAPFAVTSSVAYAEQ
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHH
LHGKELSYNNINDADAALSIVKEFTEPAVVAVKHMNPCGVGVGTDIHEAYTRAYEADPVS
HCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCHH
IFGGIIAANREIDKATAEKLHEIFLEIIIAPSFSKEALEVLQSKKNLRLLTVNIEKATSA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEEEHHHHCH
SKKLTSVQGGLLVQEEDTLSLDESTISIPTKREPSEQEWKDLKLAWKVVKHVKSNAIVLA
HHHHHHHCCCEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
KDDMTIGVGAGQMNRVGSAKIAITQAGEKAQGSALASDAFFPMPDTLEEAAKAGITAIIQ
ECCEEEEECCCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCCHHHHHHHHCCCEEEEC
PGGSIRDEDSIKVADTYGIAMVFTGVRHFKH
CCCCCCCCCCEEEEHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA