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The map label for this gene is purN [H]

Identifier: 222094056

GI number: 222094056

Start: 325280

End: 325867

Strand: Direct

Name: purN [H]

Synonym: BCQ_0349

Alternate gene names: 222094056

Gene position: 325280-325867 (Clockwise)

Preceding gene: 222094055

Following gene: 222094057

Centisome position: 6.24

GC content: 36.39

Gene sequence:

>588_bases
ATGAGTAGATTAGCAGTTTTTGCTTCTGGAAGCGGATCTAACTTTCAATCTCTCGTTAATGCAGTAGAAGAAAAAAGATT
GGATGCAGAAATTAGTTTATTAGTATGTGATAAACCAGAAGCACGCGCTGTTGGGCGAGCACATTATCATCATATCCCGT
GTTTCGCTTTTTCAGCGAAAGCATATGAGTCAAAAGAAGCGTTTGAAAAAGAGATATTAAAGAAGCTAGAAGAGTATGAA
ATTGATTATGTTATTTTAGCTGGATATATGCGTTTAATTGGACCAACTTTACTAGAAGCTTACGGCGGAAGGATTATTAA
TATTCATCCATCACTATTACCGAGTTTTCCAGGGAAAGATGCTGTTGGTCAAGCGTTAGAAGCAGGTGTGAAAGTAACTG
GAGTAACGATTCATTATGTAGACGCAGGTATGGATACAGGGCCAATTATTGCACAAGAAGCAGTAGTTGTTTCTGAAGGG
GATACGAGAGAAAGCTTACAAAAGAAAATTCAACAAGTTGAACATAAATTATACGTAAATACAGTGAATCAAATTGTTCA
ATCTGTGAAAGAACCAACTGTTAACTAA

Upstream 100 bases:

>100_bases
AAGATGTTGTTCGTCTTCTTGAAGAGCAAGGAGAAACTGCTCGTATTATTGGACGCACTGTACAAGGAGCTGGCGTTACT
TTTAATGGAGGCACAGCGCT

Downstream 100 bases:

>100_bases
CATACAACCAGGGGTGAAGGATAAATGAAAAAGCGTGCATTAGTAAGTGTTTCAGATAAAACAGGAGTAGTAGAATTTGT
TAAAGGTTTACTAGAACAAG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MSRLAVFASGSGSNFQSLVNAVEEKRLDAEISLLVCDKPEARAVGRAHYHHIPCFAFSAKAYESKEAFEKEILKKLEEYE
IDYVILAGYMRLIGPTLLEAYGGRIINIHPSLLPSFPGKDAVGQALEAGVKVTGVTIHYVDAGMDTGPIIAQEAVVVSEG
DTRESLQKKIQQVEHKLYVNTVNQIVQSVKEPTVN

Sequences:

>Translated_195_residues
MSRLAVFASGSGSNFQSLVNAVEEKRLDAEISLLVCDKPEARAVGRAHYHHIPCFAFSAKAYESKEAFEKEILKKLEEYE
IDYVILAGYMRLIGPTLLEAYGGRIINIHPSLLPSFPGKDAVGQALEAGVKVTGVTIHYVDAGMDTGPIIAQEAVVVSEG
DTRESLQKKIQQVEHKLYVNTVNQIVQSVKEPTVN
>Mature_194_residues
SRLAVFASGSGSNFQSLVNAVEEKRLDAEISLLVCDKPEARAVGRAHYHHIPCFAFSAKAYESKEAFEKEILKKLEEYEI
DYVILAGYMRLIGPTLLEAYGGRIINIHPSLLPSFPGKDAVGQALEAGVKVTGVTIHYVDAGMDTGPIIAQEAVVVSEGD
TRESLQKKIQQVEHKLYVNTVNQIVQSVKEPTVN

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=177, Percent_Identity=41.2429378531073, Blast_Score=150, Evalue=7e-37,
Organism=Homo sapiens, GI209869995, Length=177, Percent_Identity=41.2429378531073, Blast_Score=150, Evalue=7e-37,
Organism=Homo sapiens, GI209869993, Length=177, Percent_Identity=41.2429378531073, Blast_Score=150, Evalue=7e-37,
Organism=Escherichia coli, GI1788846, Length=175, Percent_Identity=35.4285714285714, Blast_Score=137, Evalue=4e-34,
Organism=Escherichia coli, GI1787483, Length=172, Percent_Identity=30.2325581395349, Blast_Score=91, Evalue=4e-20,
Organism=Escherichia coli, GI1789683, Length=118, Percent_Identity=28.8135593220339, Blast_Score=61, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17567511, Length=189, Percent_Identity=35.4497354497354, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320616, Length=215, Percent_Identity=26.046511627907, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24582400, Length=187, Percent_Identity=37.4331550802139, Blast_Score=144, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 21372; Mature: 21241

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLAVFASGSGSNFQSLVNAVEEKRLDAEISLLVCDKPEARAVGRAHYHHIPCFAFSAK
CCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEECHH
AYESKEAFEKEILKKLEEYEIDYVILAGYMRLIGPTLLEAYGGRIINIHPSLLPSFPGKD
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEECHHHCCCCCCCH
AVGQALEAGVKVTGVTIHYVDAGMDTGPIIAQEAVVVSEGDTRESLQKKIQQVEHKLYVN
HHHHHHHCCCEEEEEEEEEEECCCCCCCCEECCEEEEECCCCHHHHHHHHHHHHHHHHHH
TVNQIVQSVKEPTVN
HHHHHHHHHHCCCCC
>Mature Secondary Structure 
SRLAVFASGSGSNFQSLVNAVEEKRLDAEISLLVCDKPEARAVGRAHYHHIPCFAFSAK
CCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEECHH
AYESKEAFEKEILKKLEEYEIDYVILAGYMRLIGPTLLEAYGGRIINIHPSLLPSFPGKD
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEECHHHCCCCCCCH
AVGQALEAGVKVTGVTIHYVDAGMDTGPIIAQEAVVVSEGDTRESLQKKIQQVEHKLYVN
HHHHHHHCCCEEEEEEEEEEECCCCCCCCEECCEEEEECCCCHHHHHHHHHHHHHHHHHH
TVNQIVQSVKEPTVN
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]