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The map label for this gene is vanS [H]

Identifier: 222094029

GI number: 222094029

Start: 270611

End: 272116

Strand: Direct

Name: vanS [H]

Synonym: BCQ_0302

Alternate gene names: 222094029

Gene position: 270611-272116 (Clockwise)

Preceding gene: 222094028

Following gene: 222094030

Centisome position: 5.19

GC content: 34.53

Gene sequence:

>1506_bases
TTGAAAAAGATATTTAATCCGTTTACTTATATAAGGAAAACAAGACAATTGATTGAGAAATTAGTAAAGAGCGTACGAAA
GAGTATAAGAATTCAACTGATTACTACTTTTGCTGCTTGTGCGTTATTAGGAGTTTTTTTTGCAAAGGGAGCTGCACCGT
TTTTTGAGAATGCAAATCGTCAAGCGACTGTTGATTATCGGTCTGGTATGCAACAAATTAATTACCAAGCTCAAAGAGCA
GCAAATAGTTCGGTTCATGAAAATAAATTAGAAGCTATATCCAATATGATCGAAACGGAGAATCAAAATTTAGAGCGAGG
AAATAGAGCTTTAAAAATATTGGTTACTGATGAGAGTGGTAAAGTTGTATATAAAACGAAGCAGGCACAAGAAGAGCAAA
TCGATCTGCATAATACGATTCGTAATGCAGCATCATTTGCGATTAACTATTCAAATGGTCAAGATATATTTGAAAATACG
AGAAAAGAATTTATAGCTTTTTCACCTATTACAATAGAAAATAAGAATTTATATATGTTCGTTAGTGGAATTCCGGATGG
TGTAGTAATGTATGATACGCAAGAAGGCCCATTCCCATTTTTAATTGGTGTACTCGTATTCATTTTCTCTTTCTTCTATA
TAACAAAGAGAAAGATGAAACAAATTGAAGCGATGGCAGAAGGTGTAAAGGAAATAGAAAAAGGTAACTTAGCGTACCGC
ATTGAGAAGAAAGGTGAAGATGAGATTGCGTCTTTAACTGAGAATATTAATAATATGGCGGAAGAGCTTATGAATAATAT
AGAAAAGGAACGTAAATTAGAGAAGCAAAAGAACGAGCTTATTACAAATGTATCTCACGATTTGCGTACACCACTGACTT
CTATTATGGGTTACTTGCGATTACTTCGAGATTCTAAATATGAAAATAAAGAGCAACACGATGAATATACGAGAATTGCT
TTTGCGAAGTCAGAGCAGTTAAAGAATTTAATAGAAGATTTATTTGAGTATACGAAGTTAACGAATGAACAAGTTGTATT
AGAAAAACAAGAAGTATGTGTAAATGAGTTGCTCGAGCAATTAATAGAAGAGTTAGTACCGCAGGCGGAAGAGCATGGAC
TTACGTTCGTTAAGAAGTTTCCTGAGGAACGTGCCTATGCAGCGATTGATTCGGAAAAGATGGTTCGTGTATTTGATAAT
TTATTAATGAACGCGATTAAGTATAGTAAAGATGATGGGGAGATAAAAGTTTCTCTTCAAAGGCAGCGCCGGGATATACA
AATTGTAATTGCGAATCATAGTGAAGCGTTTACGAGAGAAGAGTTAGCGAGTTTGTTTGAACGCTTTTATAAGAAAGATC
AATCTAGAAGTAGAGTAACTGAAGGGTCGGGTCTTGGATTGGCGATTGCGAAAAGTATTGTTGAATTACAAGGTGGTAGT
ATTCGAGCTGAATATGAGGATGGTATTATTCAGTTTATCGTCTCGTTGCCAATTATAGAAAAATAA

Upstream 100 bases:

>100_bases
AATACTGTAATGGTGCATATTCGAAAAGTGCGTGAAAAGATTGAGGAGAATCCAAGGAAACCTAGATATATAAAAACAGT
ATGGGGAGTGGGGTATAAGA

Downstream 100 bases:

>100_bases
TAAACGGATAGAATGGTATATGATAAAAAGGCTTATTTTAAAAGAAATAAGCCTTTTTATATTTTTTTAGAAAAACATGT
TGACGATTAGAGATTAGAGG

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MKKIFNPFTYIRKTRQLIEKLVKSVRKSIRIQLITTFAACALLGVFFAKGAAPFFENANRQATVDYRSGMQQINYQAQRA
ANSSVHENKLEAISNMIETENQNLERGNRALKILVTDESGKVVYKTKQAQEEQIDLHNTIRNAASFAINYSNGQDIFENT
RKEFIAFSPITIENKNLYMFVSGIPDGVVMYDTQEGPFPFLIGVLVFIFSFFYITKRKMKQIEAMAEGVKEIEKGNLAYR
IEKKGEDEIASLTENINNMAEELMNNIEKERKLEKQKNELITNVSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEYTRIA
FAKSEQLKNLIEDLFEYTKLTNEQVVLEKQEVCVNELLEQLIEELVPQAEEHGLTFVKKFPEERAYAAIDSEKMVRVFDN
LLMNAIKYSKDDGEIKVSLQRQRRDIQIVIANHSEAFTREELASLFERFYKKDQSRSRVTEGSGLGLAIAKSIVELQGGS
IRAEYEDGIIQFIVSLPIIEK

Sequences:

>Translated_501_residues
MKKIFNPFTYIRKTRQLIEKLVKSVRKSIRIQLITTFAACALLGVFFAKGAAPFFENANRQATVDYRSGMQQINYQAQRA
ANSSVHENKLEAISNMIETENQNLERGNRALKILVTDESGKVVYKTKQAQEEQIDLHNTIRNAASFAINYSNGQDIFENT
RKEFIAFSPITIENKNLYMFVSGIPDGVVMYDTQEGPFPFLIGVLVFIFSFFYITKRKMKQIEAMAEGVKEIEKGNLAYR
IEKKGEDEIASLTENINNMAEELMNNIEKERKLEKQKNELITNVSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEYTRIA
FAKSEQLKNLIEDLFEYTKLTNEQVVLEKQEVCVNELLEQLIEELVPQAEEHGLTFVKKFPEERAYAAIDSEKMVRVFDN
LLMNAIKYSKDDGEIKVSLQRQRRDIQIVIANHSEAFTREELASLFERFYKKDQSRSRVTEGSGLGLAIAKSIVELQGGS
IRAEYEDGIIQFIVSLPIIEK
>Mature_501_residues
MKKIFNPFTYIRKTRQLIEKLVKSVRKSIRIQLITTFAACALLGVFFAKGAAPFFENANRQATVDYRSGMQQINYQAQRA
ANSSVHENKLEAISNMIETENQNLERGNRALKILVTDESGKVVYKTKQAQEEQIDLHNTIRNAASFAINYSNGQDIFENT
RKEFIAFSPITIENKNLYMFVSGIPDGVVMYDTQEGPFPFLIGVLVFIFSFFYITKRKMKQIEAMAEGVKEIEKGNLAYR
IEKKGEDEIASLTENINNMAEELMNNIEKERKLEKQKNELITNVSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEYTRIA
FAKSEQLKNLIEDLFEYTKLTNEQVVLEKQEVCVNELLEQLIEELVPQAEEHGLTFVKKFPEERAYAAIDSEKMVRVFDN
LLMNAIKYSKDDGEIKVSLQRQRRDIQIVIANHSEAFTREELASLFERFYKKDQSRSRVTEGSGLGLAIAKSIVELQGGS
IRAEYEDGIIQFIVSLPIIEK

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=281, Percent_Identity=25.9786476868327, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786783, Length=308, Percent_Identity=25.6493506493506, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788713, Length=249, Percent_Identity=28.5140562248996, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1790346, Length=284, Percent_Identity=25.3521126760563, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1787894, Length=338, Percent_Identity=27.5147928994083, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI145693157, Length=247, Percent_Identity=27.9352226720648, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1789149, Length=320, Percent_Identity=25.9375, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786912, Length=281, Percent_Identity=25.2669039145907, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1786600, Length=210, Percent_Identity=27.6190476190476, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87082128, Length=309, Percent_Identity=28.4789644012945, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI48994928, Length=217, Percent_Identity=26.2672811059908, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788549, Length=283, Percent_Identity=24.0282685512367, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=26.431718061674, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788279, Length=254, Percent_Identity=26.3779527559055, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789808, Length=298, Percent_Identity=26.1744966442953, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790861, Length=408, Percent_Identity=23.0392156862745, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI87081816, Length=234, Percent_Identity=23.5042735042735, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 57694; Mature: 57694

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIFNPFTYIRKTRQLIEKLVKSVRKSIRIQLITTFAACALLGVFFAKGAAPFFENANR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC
QATVDYRSGMQQINYQAQRAANSSVHENKLEAISNMIETENQNLERGNRALKILVTDESG
EEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHCCCCEEEEEEECCCC
KVVYKTKQAQEEQIDLHNTIRNAASFAINYSNGQDIFENTRKEFIAFSPITIENKNLYMF
CEEEECCHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHEEECCEEEECCCEEEE
VSGIPDGVVMYDTQEGPFPFLIGVLVFIFSFFYITKRKMKQIEAMAEGVKEIEKGNLAYR
EECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IEKKGEDEIASLTENINNMAEELMNNIEKERKLEKQKNELITNVSHDLRTPLTSIMGYLR
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLRDSKYENKEQHDEYTRIAFAKSEQLKNLIEDLFEYTKLTNEQVVLEKQEVCVNELLEQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LIEELVPQAEEHGLTFVKKFPEERAYAAIDSEKMVRVFDNLLMNAIKYSKDDGEIKVSLQ
HHHHHCCCHHHCCCHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
RQRRDIQIVIANHSEAFTREELASLFERFYKKDQSRSRVTEGSGLGLAIAKSIVELQGGS
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHCCCC
IRAEYEDGIIQFIVSLPIIEK
EEEHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKKIFNPFTYIRKTRQLIEKLVKSVRKSIRIQLITTFAACALLGVFFAKGAAPFFENANR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC
QATVDYRSGMQQINYQAQRAANSSVHENKLEAISNMIETENQNLERGNRALKILVTDESG
EEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHCCCCEEEEEEECCCC
KVVYKTKQAQEEQIDLHNTIRNAASFAINYSNGQDIFENTRKEFIAFSPITIENKNLYMF
CEEEECCHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHEEECCEEEECCCEEEE
VSGIPDGVVMYDTQEGPFPFLIGVLVFIFSFFYITKRKMKQIEAMAEGVKEIEKGNLAYR
EECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IEKKGEDEIASLTENINNMAEELMNNIEKERKLEKQKNELITNVSHDLRTPLTSIMGYLR
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLRDSKYENKEQHDEYTRIAFAKSEQLKNLIEDLFEYTKLTNEQVVLEKQEVCVNELLEQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LIEELVPQAEEHGLTFVKKFPEERAYAAIDSEKMVRVFDNLLMNAIKYSKDDGEIKVSLQ
HHHHHCCCHHHCCCHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
RQRRDIQIVIANHSEAFTREELASLFERFYKKDQSRSRVTEGSGLGLAIAKSIVELQGGS
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHCCCC
IRAEYEDGIIQFIVSLPIIEK
EEEHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]