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The map label for this gene is oppA [H]

Identifier: 222093962

GI number: 222093962

Start: 197134

End: 198774

Strand: Reverse

Name: oppA [H]

Synonym: BCQ_0218

Alternate gene names: 222093962

Gene position: 198774-197134 (Counterclockwise)

Preceding gene: 222093963

Following gene: 222093961

Centisome position: 3.81

GC content: 35.71

Gene sequence:

>1641_bases
ATGAAAAAGAAGTTTGTACCCGGTATTGCATCAGTTGTAGGAGTAAGTATTTTATTAACTGGTTGCGGTAGTTATAAAAA
CGAAGCAAGCGGAGCAAATGCAAAAGACGAGGCACCTAGTAAACAGGTTTTAAACTTATCTTCACCTACTGAAATACGAA
CAATGGATACAGCTCGTGCTACAGATACTGATTCTGGTCAAGTAATGAGAAACGTATTTGAAGGTTTGTATAACCTTGGT
GAAGGTAATAAACCTGTCCCTGGTGTTGCAAAATCTCATGAAGTAAGTGGCGATAAAACGAAATACACATTCCACCTGCG
AGATTCAAAATGGTCAAACGGTACTCCTGTTACAGCGAAAGATTTTGTCTTCGCTTGGCAAAGAGCTGTCGATCCAGCAA
CAGCCTCTGAATATGCATTTCTATTCTTTGATATTAAAAATGCAACAAAAATTAACAACAAAGAACTTCCAGCCGACCAA
CTTGGTGTAAAAGCAGTTGATGACCATACGTTTGAGGTAGAATTAGAGCGTCCTGTTCCTTATTTTATTAGCTTAACAGC
GTTCCCAACATTTCTACCAATTAACGAGGAATTCTTTAAAAAACAAGGTGATAAGTACGCTTTAGAAGACAACACAATCT
TGTACAACGGCGCTTTTACACTAAGCGATTGGAAGCATGAACAAAGCTTTAAATTCAAGAAAAACCCTACCTATTGGGAT
AAAGATACTGTCAAACTAGAAGAAATCAATTTTAACGTTGTAAAAGAAAAATCAACAGAAGTAAACTTATTCGAATCAAA
ACAATTAGACCGTATAAAATTAACATCTGATTTCGTTGATAAATATAAAAAGGATGCAAACTTTAAAGAACGCCCGAACG
TAGGCGTACAATTTTTACGTATGAATCAACAAAACAAAGTACTGCAAAACGTTTCTGCACGCCAAGCAATTGATCAAACA
ATTGATCGAAAATCCTTTGTAAATACGTTATTAAATGATGGCTCTACACCAACCTTTGGTCTCGTTCCAAAAAACTTTGC
AAAAGGGCCTGATGGAAAGGACTTCCGTACTATAAATGGAGATTTAACTAAAGTTGATACAAAATCTGCACAAGAGTTAT
GGAAAAAGGCAAAACAAGAGCTTGGTTCTGAAAAGATTACATTAGAATTATTAACGAGTGATGCTGACCTTGATAAGAAA
ACAGGTGAGTTCTTAAAAGGACAACTAGAAAAGAATTTAGATGGATTAACAGTAAATGTGAAACCACAACCACGTAAACA
ACAAGTTTCACTTTTATTAAAAGGTGACTATGAAATCGGTATTGATGGCTGGAGCCCTGACTTTGCTGATCCAATTACAT
TCCTTGAATTGTTTACAACGAATAACCCTTACAACTTGGATCATTACTCTAATAAGGAGTTCGATGAAACAATTGAAAAA
GTGAAAACGACTTTAGCTGGTGATGAAAAAGCTCGCTGGGAAGCATTACTAGCATCTGAGAAAATATTATTTAAAGACTC
TGTTATCGCTCCCCTTTACCAAAAAGGCGAATCTTATTTAGAACGTTCTTATGTGAAAGGAATTGTGCAAGTAGACTTTG
CAGGCCAATTAAACTTCAAATGGGCACAAATTGAAAAATAA

Upstream 100 bases:

>100_bases
TGTACGTAAAAATCCCTCTTTCTCAATACGAAAAGAGGGATTTTTTATTTTTCATTTCCCTCATCATCATCCAAACTTAA
TTATTTAGGAGGAAAACCAA

Downstream 100 bases:

>100_bases
AAGGCAAGCTGAATCGATATTTTTCTATCTACAGTAATTATTTTATTAAAAAAATGTATTAAAAGCGGATTCTCAATAAG
AGGAAATCCGCTTTTTTAAT

Product: extracellular solute-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MKKKFVPGIASVVGVSILLTGCGSYKNEASGANAKDEAPSKQVLNLSSPTEIRTMDTARATDTDSGQVMRNVFEGLYNLG
EGNKPVPGVAKSHEVSGDKTKYTFHLRDSKWSNGTPVTAKDFVFAWQRAVDPATASEYAFLFFDIKNATKINNKELPADQ
LGVKAVDDHTFEVELERPVPYFISLTAFPTFLPINEEFFKKQGDKYALEDNTILYNGAFTLSDWKHEQSFKFKKNPTYWD
KDTVKLEEINFNVVKEKSTEVNLFESKQLDRIKLTSDFVDKYKKDANFKERPNVGVQFLRMNQQNKVLQNVSARQAIDQT
IDRKSFVNTLLNDGSTPTFGLVPKNFAKGPDGKDFRTINGDLTKVDTKSAQELWKKAKQELGSEKITLELLTSDADLDKK
TGEFLKGQLEKNLDGLTVNVKPQPRKQQVSLLLKGDYEIGIDGWSPDFADPITFLELFTTNNPYNLDHYSNKEFDETIEK
VKTTLAGDEKARWEALLASEKILFKDSVIAPLYQKGESYLERSYVKGIVQVDFAGQLNFKWAQIEK

Sequences:

>Translated_546_residues
MKKKFVPGIASVVGVSILLTGCGSYKNEASGANAKDEAPSKQVLNLSSPTEIRTMDTARATDTDSGQVMRNVFEGLYNLG
EGNKPVPGVAKSHEVSGDKTKYTFHLRDSKWSNGTPVTAKDFVFAWQRAVDPATASEYAFLFFDIKNATKINNKELPADQ
LGVKAVDDHTFEVELERPVPYFISLTAFPTFLPINEEFFKKQGDKYALEDNTILYNGAFTLSDWKHEQSFKFKKNPTYWD
KDTVKLEEINFNVVKEKSTEVNLFESKQLDRIKLTSDFVDKYKKDANFKERPNVGVQFLRMNQQNKVLQNVSARQAIDQT
IDRKSFVNTLLNDGSTPTFGLVPKNFAKGPDGKDFRTINGDLTKVDTKSAQELWKKAKQELGSEKITLELLTSDADLDKK
TGEFLKGQLEKNLDGLTVNVKPQPRKQQVSLLLKGDYEIGIDGWSPDFADPITFLELFTTNNPYNLDHYSNKEFDETIEK
VKTTLAGDEKARWEALLASEKILFKDSVIAPLYQKGESYLERSYVKGIVQVDFAGQLNFKWAQIEK
>Mature_546_residues
MKKKFVPGIASVVGVSILLTGCGSYKNEASGANAKDEAPSKQVLNLSSPTEIRTMDTARATDTDSGQVMRNVFEGLYNLG
EGNKPVPGVAKSHEVSGDKTKYTFHLRDSKWSNGTPVTAKDFVFAWQRAVDPATASEYAFLFFDIKNATKINNKELPADQ
LGVKAVDDHTFEVELERPVPYFISLTAFPTFLPINEEFFKKQGDKYALEDNTILYNGAFTLSDWKHEQSFKFKKNPTYWD
KDTVKLEEINFNVVKEKSTEVNLFESKQLDRIKLTSDFVDKYKKDANFKERPNVGVQFLRMNQQNKVLQNVSARQAIDQT
IDRKSFVNTLLNDGSTPTFGLVPKNFAKGPDGKDFRTINGDLTKVDTKSAQELWKKAKQELGSEKITLELLTSDADLDKK
TGEFLKGQLEKNLDGLTVNVKPQPRKQQVSLLLKGDYEIGIDGWSPDFADPITFLELFTTNNPYNLDHYSNKEFDETIEK
VKTTLAGDEKARWEALLASEKILFKDSVIAPLYQKGESYLERSYVKGIVQVDFAGQLNFKWAQIEK

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=528, Percent_Identity=30.4924242424242, Blast_Score=232, Evalue=6e-62,
Organism=Escherichia coli, GI87081878, Length=531, Percent_Identity=30.1318267419962, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI1789397, Length=493, Percent_Identity=28.3975659229209, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1787762, Length=508, Percent_Identity=25.7874015748031, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787052, Length=473, Percent_Identity=25.1585623678647, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789966, Length=486, Percent_Identity=23.0452674897119, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61650; Mature: 61650

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKFVPGIASVVGVSILLTGCGSYKNEASGANAKDEAPSKQVLNLSSPTEIRTMDTARA
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHEEECCCCCCEEEECCCCC
TDTDSGQVMRNVFEGLYNLGEGNKPVPGVAKSHEVSGDKTKYTFHLRDSKWSNGTPVTAK
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHH
DFVFAWQRAVDPATASEYAFLFFDIKNATKINNKELPADQLGVKAVDDHTFEVELERPVP
HHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHCCCEEECCCEEEEEECCCCC
YFISLTAFPTFLPINEEFFKKQGDKYALEDNTILYNGAFTLSDWKHEQSFKFKKNPTYWD
EEEEEECCCEECCCCHHHHHCCCCCEEECCCEEEEECEEEECCCCCCCCCEECCCCCCCC
KDTVKLEEINFNVVKEKSTEVNLFESKQLDRIKLTSDFVDKYKKDANFKERPNVGVQFLR
CCCEEEEECCEEEEECCCCEEEEECCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCEEEEE
MNQQNKVLQNVSARQAIDQTIDRKSFVNTLLNDGSTPTFGLVPKNFAKGPDGKDFRTING
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHCCCCCCCCEEEECC
DLTKVDTKSAQELWKKAKQELGSEKITLELLTSDADLDKKTGEFLKGQLEKNLDGLTVNV
CEEECCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEE
KPQPRKQQVSLLLKGDYEIGIDGWSPDFADPITFLELFTTNNPYNLDHYSNKEFDETIEK
CCCCCHHEEEEEEECCEEEECCCCCCCCCCHHEEEEEEECCCCCCCCCCCCCCHHHHHHH
VKTTLAGDEKARWEALLASEKILFKDSVIAPLYQKGESYLERSYVKGIVQVDFAGQLNFK
HHHHHCCCCHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEE
WAQIEK
EEECCC
>Mature Secondary Structure
MKKKFVPGIASVVGVSILLTGCGSYKNEASGANAKDEAPSKQVLNLSSPTEIRTMDTARA
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHEEECCCCCCEEEECCCCC
TDTDSGQVMRNVFEGLYNLGEGNKPVPGVAKSHEVSGDKTKYTFHLRDSKWSNGTPVTAK
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHH
DFVFAWQRAVDPATASEYAFLFFDIKNATKINNKELPADQLGVKAVDDHTFEVELERPVP
HHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHCCCEEECCCEEEEEECCCCC
YFISLTAFPTFLPINEEFFKKQGDKYALEDNTILYNGAFTLSDWKHEQSFKFKKNPTYWD
EEEEEECCCEECCCCHHHHHCCCCCEEECCCEEEEECEEEECCCCCCCCCEECCCCCCCC
KDTVKLEEINFNVVKEKSTEVNLFESKQLDRIKLTSDFVDKYKKDANFKERPNVGVQFLR
CCCEEEEECCEEEEECCCCEEEEECCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCEEEEE
MNQQNKVLQNVSARQAIDQTIDRKSFVNTLLNDGSTPTFGLVPKNFAKGPDGKDFRTING
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHCCCCCCCCEEEECC
DLTKVDTKSAQELWKKAKQELGSEKITLELLTSDADLDKKTGEFLKGQLEKNLDGLTVNV
CEEECCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEE
KPQPRKQQVSLLLKGDYEIGIDGWSPDFADPITFLELFTTNNPYNLDHYSNKEFDETIEK
CCCCCHHEEEEEEECCEEEECCCCCCCCCCHHEEEEEEECCCCCCCCCCCCCCHHHHHHH
VKTTLAGDEKARWEALLASEKILFKDSVIAPLYQKGESYLERSYVKGIVQVDFAGQLNFK
HHHHHCCCCHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEE
WAQIEK
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]