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The map label for this gene is oppA [H]

Identifier: 222093961

GI number: 222093961

Start: 195106

End: 196746

Strand: Reverse

Name: oppA [H]

Synonym: BCQ_0217

Alternate gene names: 222093961

Gene position: 196746-195106 (Counterclockwise)

Preceding gene: 222093962

Following gene: 222093957

Centisome position: 3.77

GC content: 36.99

Gene sequence:

>1641_bases
ATGAAGAAAAAAGTTGTACCTGTTGTTGCATCTGTTTTAGGGGCAAGTCTATTACTAACTGCTTGCGGGGGAAATAAAGA
TAACGCAAGCGGAGCGAAAGCAAATGATAAAGCACCTGATAAACAAGCAATTAACTTATCATTTGCTTCAGAAATTCCAA
CAATGGATGTTGCAAAAGCAACGGATGGGGAATCCATGAACGTAATGCGTAATGTTTTTGAAGGGTTATATGCAATGGGA
GAAGATAATAAACCGATTCCTGGTGTCGCTGAGTCGGTTGACGTTAGTCCCGATAAAACGAAATATACATTCCATCTGCG
TGATTCAAAATGGTCAAACGGTACTCCTGTTACAGCAAAAGACTTTGTCTTCGCTTGGCAACGTGCCGTAAACCCTGATA
CAGCAGCTGAATATGCATTCTTATTCTTCGATATAAAGAATGCGAAACAAATAAACCAAAAACAATTGCCGATTGATCAA
CTAGGGGTCAAAGCTATTGATGACAAAACATTAGAAGTACAACTAGATCGTCCTGTTCCCTACTTCTTAAGTTTAACGAC
ATTCTCAACATTCTTACCAATTAATGAAGAGTACTTAAAGTCTCAAGGCGATAAATACGGTTTAGAAACAAATCATTTAA
TTTACAACGGTGCCTTCACATTAGATAACTGGAAACACGAGCAAAGCTTCCAACTGAAGAAGAACCCTAACTATTGGGAT
GCTAAAACGGTGAAATTAGAAGAAATCAACTTCAATGTCGTTAAAGATAAGTCTACAGAAGTTAATTTATATGATTCAGG
ACAAATTGATCGCGTTGCTTTAACTGCGGAGTTTGTTGATAAATATAAGGGCGATCCAAACTTCAAAGAACGCGCTGAAG
TTGGCATTCAATTTTTACGACTCAATCAAAAAAATGAAACATTAAAAAATCAACATGCACGCCTTGCTATTAACGGAGCA
ATGAATAAGAAAGCATACGTAGAAACAATTTTAAATAACGGCGCTGTTGCAGCTGAAGGAATGGTTCCTGCGAAATTTGC
AAAAAGTCCTGATGGCAACGACTTCCGTAAAGAAAATGGAAATCTAGTGAAAGATGATGTGAAAACAGCGAAAGAAAACT
GGAAAAAAGCGAAGCAAGAACTGGGTACTGACAAAGTAACAATTGAACTCTTAACAAGTGATAATGCTTTAGCTAAAAAG
ACTGGTGAATATTTAAAAGGTGAACTAGAAAAGAACTTAGATGGATTAACAGTAAATTTAAAACCACAACCACGTAAACA
ACAGTTAAAACTACTGTTAAGTGGTGACTATGAAATCGGTATTGATGGCTGGGGCCCTGACTTCGCTGATCCAATTACAT
TCTTAGATTTATTTACAACAGATAGTGCTTACAACTTCGATAAATACTCTAACAAAGAGTACGATGAGCTAATTCATAAA
GTAAAAACAGATTTAGCTGGTGATGAAAAAGCTCGCTGGGAAGCAATGAAGCAAGCTGAAAAAATTCTATTACAAGACGG
TGCTGTCGCTCCTTTATACCAACAAGGTCGTTCTTACTTACAACGCTCTTTCATTAAAGGACTTGTAACAACTGACTTCG
GTGGTGAGTTTAACTACAAGTGGACAGAAGTTGCAAAATAA

Upstream 100 bases:

>100_bases
ATGTACATATTTATAATAGGTTTTCTCTTCTTCTATAGATACACTTCAAAACATGGACATTATGACGTTACGAAACAACA
CATTTAGGGAGGAACGAACA

Downstream 100 bases:

>100_bases
TAAGTGCTAATAAAAAGGTATCCCACCGAGGATACCTTTTTATTTTATGCTTCTTTAACTACAGAGCTTCTTCTTCCCTT
TACCATTCCTTTTATGGCTA

Product: extracellular solute-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MKKKVVPVVASVLGASLLLTACGGNKDNASGAKANDKAPDKQAINLSFASEIPTMDVAKATDGESMNVMRNVFEGLYAMG
EDNKPIPGVAESVDVSPDKTKYTFHLRDSKWSNGTPVTAKDFVFAWQRAVNPDTAAEYAFLFFDIKNAKQINQKQLPIDQ
LGVKAIDDKTLEVQLDRPVPYFLSLTTFSTFLPINEEYLKSQGDKYGLETNHLIYNGAFTLDNWKHEQSFQLKKNPNYWD
AKTVKLEEINFNVVKDKSTEVNLYDSGQIDRVALTAEFVDKYKGDPNFKERAEVGIQFLRLNQKNETLKNQHARLAINGA
MNKKAYVETILNNGAVAAEGMVPAKFAKSPDGNDFRKENGNLVKDDVKTAKENWKKAKQELGTDKVTIELLTSDNALAKK
TGEYLKGELEKNLDGLTVNLKPQPRKQQLKLLLSGDYEIGIDGWGPDFADPITFLDLFTTDSAYNFDKYSNKEYDELIHK
VKTDLAGDEKARWEAMKQAEKILLQDGAVAPLYQQGRSYLQRSFIKGLVTTDFGGEFNYKWTEVAK

Sequences:

>Translated_546_residues
MKKKVVPVVASVLGASLLLTACGGNKDNASGAKANDKAPDKQAINLSFASEIPTMDVAKATDGESMNVMRNVFEGLYAMG
EDNKPIPGVAESVDVSPDKTKYTFHLRDSKWSNGTPVTAKDFVFAWQRAVNPDTAAEYAFLFFDIKNAKQINQKQLPIDQ
LGVKAIDDKTLEVQLDRPVPYFLSLTTFSTFLPINEEYLKSQGDKYGLETNHLIYNGAFTLDNWKHEQSFQLKKNPNYWD
AKTVKLEEINFNVVKDKSTEVNLYDSGQIDRVALTAEFVDKYKGDPNFKERAEVGIQFLRLNQKNETLKNQHARLAINGA
MNKKAYVETILNNGAVAAEGMVPAKFAKSPDGNDFRKENGNLVKDDVKTAKENWKKAKQELGTDKVTIELLTSDNALAKK
TGEYLKGELEKNLDGLTVNLKPQPRKQQLKLLLSGDYEIGIDGWGPDFADPITFLDLFTTDSAYNFDKYSNKEYDELIHK
VKTDLAGDEKARWEAMKQAEKILLQDGAVAPLYQQGRSYLQRSFIKGLVTTDFGGEFNYKWTEVAK
>Mature_546_residues
MKKKVVPVVASVLGASLLLTACGGNKDNASGAKANDKAPDKQAINLSFASEIPTMDVAKATDGESMNVMRNVFEGLYAMG
EDNKPIPGVAESVDVSPDKTKYTFHLRDSKWSNGTPVTAKDFVFAWQRAVNPDTAAEYAFLFFDIKNAKQINQKQLPIDQ
LGVKAIDDKTLEVQLDRPVPYFLSLTTFSTFLPINEEYLKSQGDKYGLETNHLIYNGAFTLDNWKHEQSFQLKKNPNYWD
AKTVKLEEINFNVVKDKSTEVNLYDSGQIDRVALTAEFVDKYKGDPNFKERAEVGIQFLRLNQKNETLKNQHARLAINGA
MNKKAYVETILNNGAVAAEGMVPAKFAKSPDGNDFRKENGNLVKDDVKTAKENWKKAKQELGTDKVTIELLTSDNALAKK
TGEYLKGELEKNLDGLTVNLKPQPRKQQLKLLLSGDYEIGIDGWGPDFADPITFLDLFTTDSAYNFDKYSNKEYDELIHK
VKTDLAGDEKARWEAMKQAEKILLQDGAVAPLYQQGRSYLQRSFIKGLVTTDFGGEFNYKWTEVAK

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=544, Percent_Identity=31.25, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI87081878, Length=516, Percent_Identity=30.2325581395349, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1789397, Length=521, Percent_Identity=27.2552783109405, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1787762, Length=453, Percent_Identity=25.60706401766, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1789966, Length=470, Percent_Identity=23.8297872340426, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1787551, Length=500, Percent_Identity=22.4, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787052, Length=489, Percent_Identity=23.5173824130879, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789887, Length=482, Percent_Identity=25.103734439834, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61177; Mature: 61177

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKVVPVVASVLGASLLLTACGGNKDNASGAKANDKAPDKQAINLSFASEIPTMDVAKA
CCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEECC
TDGESMNVMRNVFEGLYAMGEDNKPIPGVAESVDVSPDKTKYTFHLRDSKWSNGTPVTAK
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHH
DFVFAWQRAVNPDTAAEYAFLFFDIKNAKQINQKQLPIDQLGVKAIDDKTLEVQLDRPVP
HHHHHHHHCCCCCCHHCEEEEEEEECCHHHCCHHCCCHHHHCCEEECCCEEEEEECCCCC
YFLSLTTFSTFLPINEEYLKSQGDKYGLETNHLIYNGAFTLDNWKHEQSFQLKKNPNYWD
CEEEHHHHHHHCCCCHHHHHCCCCCCCCCCCEEEEECEEEECCCCCCCCEEECCCCCCCC
AKTVKLEEINFNVVKDKSTEVNLYDSGQIDRVALTAEFVDKYKGDPNFKERAEVGIQFLR
CEEEEEEECCEEEEECCCCEEEEECCCCCCEEEEEHHHHHHHCCCCCHHHHHHHCEEEEE
LNQKNETLKNQHARLAINGAMNKKAYVETILNNGAVAAEGMVPAKFAKSPDGNDFRKENG
ECCCCHHHHCCCCEEEEECCCCCHHHHHHHHCCCCEEECCCCCHHHCCCCCCCCHHHCCC
NLVKDDVKTAKENWKKAKQELGTDKVTIELLTSDNALAKKTGEYLKGELEKNLDGLTVNL
CEEHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEE
KPQPRKQQLKLLLSGDYEIGIDGWGPDFADPITFLDLFTTDSAYNFDKYSNKEYDELIHK
CCCCHHHHEEEEEECCEEEECCCCCCCCCCHHHHHHEEECCCCCCCCCCCCCHHHHHHHH
VKTDLAGDEKARWEAMKQAEKILLQDGAVAPLYQQGRSYLQRSFIKGLVTTDFGGEFNYK
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCE
WTEVAK
EHCCCC
>Mature Secondary Structure
MKKKVVPVVASVLGASLLLTACGGNKDNASGAKANDKAPDKQAINLSFASEIPTMDVAKA
CCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEECC
TDGESMNVMRNVFEGLYAMGEDNKPIPGVAESVDVSPDKTKYTFHLRDSKWSNGTPVTAK
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHH
DFVFAWQRAVNPDTAAEYAFLFFDIKNAKQINQKQLPIDQLGVKAIDDKTLEVQLDRPVP
HHHHHHHHCCCCCCHHCEEEEEEEECCHHHCCHHCCCHHHHCCEEECCCEEEEEECCCCC
YFLSLTTFSTFLPINEEYLKSQGDKYGLETNHLIYNGAFTLDNWKHEQSFQLKKNPNYWD
CEEEHHHHHHHCCCCHHHHHCCCCCCCCCCCEEEEECEEEECCCCCCCCEEECCCCCCCC
AKTVKLEEINFNVVKDKSTEVNLYDSGQIDRVALTAEFVDKYKGDPNFKERAEVGIQFLR
CEEEEEEECCEEEEECCCCEEEEECCCCCCEEEEEHHHHHHHCCCCCHHHHHHHCEEEEE
LNQKNETLKNQHARLAINGAMNKKAYVETILNNGAVAAEGMVPAKFAKSPDGNDFRKENG
ECCCCHHHHCCCCEEEEECCCCCHHHHHHHHCCCCEEECCCCCHHHCCCCCCCCHHHCCC
NLVKDDVKTAKENWKKAKQELGTDKVTIELLTSDNALAKKTGEYLKGELEKNLDGLTVNL
CEEHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEE
KPQPRKQQLKLLLSGDYEIGIDGWGPDFADPITFLDLFTTDSAYNFDKYSNKEYDELIHK
CCCCHHHHEEEEEECCEEEECCCCCCCCCCHHHHHHEEECCCCCCCCCCCCCHHHHHHHH
VKTDLAGDEKARWEAMKQAEKILLQDGAVAPLYQQGRSYLQRSFIKGLVTTDFGGEFNYK
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCE
WTEVAK
EHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]