Click here to switch to the map view.

The map label for this gene is mesJ [H]

Identifier: 222093833

GI number: 222093833

Start: 67333

End: 68763

Strand: Direct

Name: mesJ [H]

Synonym: BCQ_0072

Alternate gene names: 222093833

Gene position: 67333-68763 (Clockwise)

Preceding gene: 222093832

Following gene: 222093834

Centisome position: 1.29

GC content: 36.2

Gene sequence:

>1431_bases
TTGAAAGATACATTTGTTGAAAAGGTAGATGACTTTGTAAAGCAGCATGATGTATTAAAGGAACGTTCAACAATTGTTGT
AGGGGTTTCTGGTGGTCCTGACTCTTTGGCTCTTCTATATTATTTGTTAGAAAAAAGAGCAGCAAAACAGTTTGAAATTG
TAGTGGCGCATGTGGATCACATGTTTAGAGGTGATGAATCTCATGAGGACCTACAGTTTGTGCAGGACCTTTGTAAAGGA
CTGGAAGTTATTTGCGAAACGATAAGGATTAATGTGTCGCAATATCAACAGCAATACGGGATGAATGCACAGGTTGCTGC
TAGAGAATGCAGGTATGCATTTTTGGAAAGAATAATGAAGAAATATGATGCGAGATATGTAGCCCTTGGACATCATGGTG
ATGACCAAGTAGAGACGATTTTAATGCGTCTTGTACGAGGAAGTACTCCGAAAGGATATGCAGGAATTGCAGTGAAGCGT
CCTTTTCATAATGGATATTTAATTAGGCCGTTACTTGGAGTAACAAAGGAAGAAATTGTTGATTACTGTAATAAGCTGAA
AATAATTCCTCGTATAGATCCAAGTAATAAAAAGGAAGTATATACAAGGAATCGATTACGTAAATATGTTCTTCCTCATT
TGAAAGAAGAAAACCCACAAGTGCATGAGAAATTCCAAAAATTTAGCATGCAGATGCAAGAGGATGAGGCGTATTTGCAG
GAATTAGCTTTTGAGAAAATGAATAAAGTAATTACAAAAAAAAGTGATAAACAAATTAGCTTATCAATTCCTGCCTTTGA
ATCCATGTCTATGCCTTTACAAAGAAGAGGGATTCAACTAATATTAAACTATCTTTATGAATATAAGATTCCATCTTCTC
TTTCCTCTATACATATTGACAAAGTGATTGAGTTTTTTAAGCGGACACAACCTTCGGGTTCACTTGATTTCCCAGGTGAT
TTGAAAATTGTTCGCGCATATGAGGAGTGTAGCTTTGAATTTAAACAAGAAATTGTTTCTCCTTTCTTACAAGATTTATC
AGTTCCCGGAACAATTACGCTATCGAACGGGGATAAACTTGTAACAGAGGTGAGCGAAGATATACCAAGTGACATGAATG
AAACAGTATTTGTTGCTAAGTATAATGATATATCATATCCACTTCGTATTCGGTCTAGAGAAAATGGAGATCGCATGTCA
ATACAAGGTATGAATGGCACGAAAAAGATAAAAGCTATTTTTATCGAAGCAAAAGTACCAAGAGAAAAAAGAGAAGAATG
GCCGGTCGTTTGTGATGCAAGTGGGAATGTTATTTGGTTACCCTTGTTGAAGCGATCTGCATTTGCTATCTCGAAAGAGA
CAGCAAAGAAGGATAAATATATGATTATTCACTACAAAAGCAAGGAGTCTTCCGGGAGGATAATGAAATGA

Upstream 100 bases:

>100_bases
TTTGTATATCTTTTTTTTGCACAATGTTTGTAAAAGTTGTACCATTATAAACAAGGTAACTAACGTTGCTGTTCTTGTTT
AATGAAAGGTGATTACGAAG

Downstream 100 bases:

>100_bases
TGAATCAAGATATCGAAAAAGTATTAATTTCTGAAGAGCAAATACAAGAAAAGGTGCTCGAATTAGGCGCAATTATTGCA
GAGGATTATAAAAACACAGT

Product: cell cycle protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKDTFVEKVDDFVKQHDVLKERSTIVVGVSGGPDSLALLYYLLEKRAAKQFEIVVAHVDHMFRGDESHEDLQFVQDLCKG
LEVICETIRINVSQYQQQYGMNAQVAARECRYAFLERIMKKYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKR
PFHNGYLIRPLLGVTKEEIVDYCNKLKIIPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQVHEKFQKFSMQMQEDEAYLQ
ELAFEKMNKVITKKSDKQISLSIPAFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHIDKVIEFFKRTQPSGSLDFPGD
LKIVRAYEECSFEFKQEIVSPFLQDLSVPGTITLSNGDKLVTEVSEDIPSDMNETVFVAKYNDISYPLRIRSRENGDRMS
IQGMNGTKKIKAIFIEAKVPREKREEWPVVCDASGNVIWLPLLKRSAFAISKETAKKDKYMIIHYKSKESSGRIMK

Sequences:

>Translated_476_residues
MKDTFVEKVDDFVKQHDVLKERSTIVVGVSGGPDSLALLYYLLEKRAAKQFEIVVAHVDHMFRGDESHEDLQFVQDLCKG
LEVICETIRINVSQYQQQYGMNAQVAARECRYAFLERIMKKYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKR
PFHNGYLIRPLLGVTKEEIVDYCNKLKIIPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQVHEKFQKFSMQMQEDEAYLQ
ELAFEKMNKVITKKSDKQISLSIPAFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHIDKVIEFFKRTQPSGSLDFPGD
LKIVRAYEECSFEFKQEIVSPFLQDLSVPGTITLSNGDKLVTEVSEDIPSDMNETVFVAKYNDISYPLRIRSRENGDRMS
IQGMNGTKKIKAIFIEAKVPREKREEWPVVCDASGNVIWLPLLKRSAFAISKETAKKDKYMIIHYKSKESSGRIMK
>Mature_476_residues
MKDTFVEKVDDFVKQHDVLKERSTIVVGVSGGPDSLALLYYLLEKRAAKQFEIVVAHVDHMFRGDESHEDLQFVQDLCKG
LEVICETIRINVSQYQQQYGMNAQVAARECRYAFLERIMKKYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKR
PFHNGYLIRPLLGVTKEEIVDYCNKLKIIPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQVHEKFQKFSMQMQEDEAYLQ
ELAFEKMNKVITKKSDKQISLSIPAFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHIDKVIEFFKRTQPSGSLDFPGD
LKIVRAYEECSFEFKQEIVSPFLQDLSVPGTITLSNGDKLVTEVSEDIPSDMNETVFVAKYNDISYPLRIRSRENGDRMS
IQGMNGTKKIKAIFIEAKVPREKREEWPVVCDASGNVIWLPLLKRSAFAISKETAKKDKYMIIHYKSKESSGRIMK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=421, Percent_Identity=27.3159144893112, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 54964; Mature: 54964

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDTFVEKVDDFVKQHDVLKERSTIVVGVSGGPDSLALLYYLLEKRAAKQFEIVVAHVDH
CCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MFRGDESHEDLQFVQDLCKGLEVICETIRINVSQYQQQYGMNAQVAARECRYAFLERIMK
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKRPFHNGYLIRPLLGVTKEEIV
HHCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEHHHHCCCHHHHH
DYCNKLKIIPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQVHEKFQKFSMQMQEDEAYLQ
HHHHHEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ELAFEKMNKVITKKSDKQISLSIPAFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHID
HHHHHHHHHHHHCCCCCEEEEECCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
KVIEFFKRTQPSGSLDFPGDLKIVRAYEECSFEFKQEIVSPFLQDLSVPGTITLSNGDKL
HHHHHHHHCCCCCCCCCCCCHHEEEHHHHHCHHHHHHHHHHHHHHCCCCCEEEECCCCHH
VTEVSEDIPSDMNETVFVAKYNDISYPLRIRSRENGDRMSIQGMNGTKKIKAIFIEAKVP
HHHHHHHCCCCCCCEEEEEEECCCCCCEEEECCCCCCEEEEECCCCCCEEEEEEEEECCC
REKREEWPVVCDASGNVIWLPLLKRSAFAISKETAKKDKYMIIHYKSKESSGRIMK
HHHHCCCCEEEECCCCEEEEEEHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MKDTFVEKVDDFVKQHDVLKERSTIVVGVSGGPDSLALLYYLLEKRAAKQFEIVVAHVDH
CCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MFRGDESHEDLQFVQDLCKGLEVICETIRINVSQYQQQYGMNAQVAARECRYAFLERIMK
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KYDARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKRPFHNGYLIRPLLGVTKEEIV
HHCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEHHHHCCCHHHHH
DYCNKLKIIPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQVHEKFQKFSMQMQEDEAYLQ
HHHHHEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ELAFEKMNKVITKKSDKQISLSIPAFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHID
HHHHHHHHHHHHCCCCCEEEEECCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
KVIEFFKRTQPSGSLDFPGDLKIVRAYEECSFEFKQEIVSPFLQDLSVPGTITLSNGDKL
HHHHHHHHCCCCCCCCCCCCHHEEEHHHHHCHHHHHHHHHHHHHHCCCCCEEEECCCCHH
VTEVSEDIPSDMNETVFVAKYNDISYPLRIRSRENGDRMSIQGMNGTKKIKAIFIEAKVP
HHHHHHHCCCCCCCEEEEEEECCCCCCEEEECCCCCCEEEEECCCCCCEEEEEEEEECCC
REKREEWPVVCDASGNVIWLPLLKRSAFAISKETAKKDKYMIIHYKSKESSGRIMK
HHHHCCCCEEEECCCCEEEEEEHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]